Figure S4. Phylogenetic tree of selected PAR1-like ...

SbPAR1d SbPAR1c ZmPAR1b SbPAR1b

SiPAR1a SbPAR1a ZmPAR1a PsPAR1a PsPAR1b PsPAR1c

SmPAR1a SmPAR1b SmPAR1c

Clade B SlPAR1d SlPAR1c

PpPAR1a

SlPAR1e

SlPAR1h

NtPAR1d

GmPAR1b GmPAR1c

SlPAR1f GmPAR1d GmPAR1e AtPAR1b SlPAR1g

AtPAR1a NtPAR1b

GmPAR1a NtPAR1c SlPAR1b SlPAR1a NtPAR1a

10

Clade A

Figure S4. Phylogenetic tree of selected PAR1-like protein sequences in plants. Alignment was performed with the signal peptides removed. The names correspond to sequence IDs listed in Table S4. Table S4. Naming and properties of PAR1-like genes: Nicotiana tabacum (tobacco), Arabidopsis thaliana (Arabidopsis), Glycine max (soybean), Solanum lycopersicum (tomato), Zea mays (maize), Sorghum bicolor (sorghum), Setaria italica (foxtail millet), Picea sitchensis (Sitka spruce), Selaginella moellendorffii (club moss) and Physcomitrella patens (moss).

Gene Name NtPAR1a NtPAR1b NtPAR1c NtPAR1d AtPAR1a AtPAR1b GmPAR1a GmPAR1b GmPAR1c GmPAR1d GmPAR1e SlPAR1a SlPAR1b SlPAR1c SlPAR1d SlPAR1e SlPAR1f SlPAR1g SlPAR1h ZmPAR1a ZmPAR1a SbPAR1a SbPAR1b SbPAR1c SbPAR1d SiPAR1a PsPAR1a PsPAR1b PsPAR1c SmPAR1a SmPAR1b SmPAR1c PpPAR1a 1 2

Accession/ID X83853 X83851 X83852 AB041515 AT5G52390 AT3G54040 Glyma09g25420.1 Glyma10g37940.1 Glyma20g29860.1 Glyma01g26840.2 Glyma03g15130.1 Solyc03g025670.2.1 Solyc03g025680.2.1 Solyc09g011790.2.1 Solyc09g011800.2.1 Solyc10g085010.1.1 Solyc10g086710.1.1 Solyc06g005920.1.1 Solyc01g099920.2.1 AFW77700 AFW78200 XM_002439588 XM_002439851 XM_002439852 XM_002439853 XP_004962356 ABK22647 ABK26953 ABK26944 XP_002971544 XP_002971753 XP_002993093 Pp1s185_4V6

Mol Wt no SP 1 (kDa) 17.4 17.5 17.1 16.2 18.6 16.7 18.9 17.8 18.0 16.7 17.0 17.4 17.5 16.3 16.2 17.2 16.2 18.3 22.6 15.8 15.2 16.2 17.8 15.3 15.3 16.8 18.1 19.7 19.8 19.3 18.4 18.4 30.3

Percent aa Similarity 2 no SP 1 100 94 89 62 73 60 75 83 81 68 68 87 87 60 59 61 62 60 46 60 52 58 38 50 49 59 59 63 62 51 52 52 36

no SP – signal peptide identified by SignalP 4.0 is removed. Percent amino acid similarity using MacVector 12.7, ClustalW.