Figure S4

Fv Ff Fo Fg Fl Nh Tv Ac Th

Fv Ff Fo Fg Fl Nh Tv Ac Th

Fv Ff Fo Fg Fl Nh Tv Ac Th

Fv Ff Fo Fg Fl Nh Tv Ac Th

Fv Ff Fo Fg Fl Nh Tv

EWG40990.1 KLP03525.1 EWZ36701.1 ESU17197.1 KPA39364.1 XP_003053980.1 XP_013952680.1 KFH42786.1 KKO99655.1

10 20 30 40 50 ....|....|....|....|....|....|....|....|....|....| MCRETITIAQCAPEVSPVLAFYCAKLHLVAKDRIICDATKGKCICFFGSC 50 MCSETITVAQCAPKVSPVLAFYCAKLHLVAKERIVCEAAKGKCICFFGSC 50 MCRETITIAQCAPEVAPVLAFYCAKLHLVAKDRTVCDAARGKCICFFGSC 50 MCVETITIALCAPQSAPRLSFTCGNLHLVAENHVVCDSAVGNCICYFGTC 50 MCVETITIALCAPESSPHLSFTCGKLHLVAGRRTVCDKASGRCVCYFGTC 50 MCHEVITIALCAPTKAPSLSFTCGKLHMIAQQRHVCDRARGSCVCFFGTC 50 MCKEFLTIAICAPQKAPSMSFTCGQLHLVAERRQVCDKATGACLCFVGTC 50 MCSEDIAVALCAPVERRALSYTCSKLHLIASNRHVCFRAKGNCVCFFGTC 50 MCKEYTTIAICAPQKAPSMSFTCGQLHQVVDRRQVCDKATGSCICFVGTC 50

EWG40990.1 KLP03525.1 EWZ36701.1 ESU17197.1 KPA39364.1 XP_003053980.1 XP_013952680.1 KFH42786.1 KKO99655.1

60 70 80 90 100 ....|....|....|....|....|....|....|....|....|....| GTIEREIPTKGINLGEVVKARCAECTPREHRVRD-IPAQEILESVLLR-S 98 GAIDREIPTKGINLSDIVKARCAECTPREHRVGNGIPAQQILESVLLR-S 99 GTIDREIPTKGINLGDVVKVRCAECTPREHRVGNGLKAQQILESVLLR-S 99 GKVIREVPTGGIDFSAISKVRCTTCTAREDEVGDARKDREILESVLLGRP 100 GNAGRAALTDGIDFSAISKVRCTSCTAREDAVGDRRKNQEVLESVLLDPS 100 GTVEKNASTSGIDLSAIEKIRCAECTTREDNVGDRRDAKQIIESPLLQRI 100 GVLESVPSTQGLRMSSVKSIRCAACTSREEEVGDRRTNEELIESPLLTRT 100 GTVERTFDS-VFDLTDLPKVRCSVCTVREDDVGHSSAAREILESPVPTRK 99 GVLETIPSTLAIPIASLKSIRCVACTTREEEIGDRRSNEELIDSPLLTRA 100

EWG40990.1 KLP03525.1 EWZ36701.1 ESU17197.1 KPA39364.1 XP_003053980.1 XP_013952680.1 KFH42786.1 KKO99655.1

110 120 130 140 150 ....|....|....|....|....|....|....|....|....|....| TDGQNG---WEERSKLLSELWLNKTACPYHVDTSAEP---VAPVINITES 142 ADAQNG---WEERSKLVSELWLNKTACPYHVDTSAEPATPVAEVINITEH 146 VDARNG---WEERSKLISELWLNKPACPYHVETSTEPAGPITGAINITQH 146 ATNDAD---WDQTSKRLQQLWQQKSACPYHVQGSVRK---AKATVNKPAK 144 AVHYVD---VDRKVKLLSQLWHQKSDCPYHAEG----------------- 130 PIEPTDDFVNKKHSAMLHELWGGARTCPFHLQPDRPR------------- 137 AIKSSEQ--KIKFDEIIKDLWNGKEDCPYHVLSTVRE------------- 135 AIPEEG---LERYMRTLKSMWGSMDHCPYHVTEHENQ--------EMAAH 138 AVKSTED--MQHFKAIIKSLWNGEDECPYHTMLTTRE------------- 135

EWG40990.1 KLP03525.1 EWZ36701.1 ESU17197.1 KPA39364.1 XP_003053980.1 XP_013952680.1 KFH42786.1 KKO99655.1

160 170 180 190 200 ....|....|....|....|....|....|....|....|....|....| IHLDTEVGHYGTGLSAESAGPTDSTPESAL-------ELKPAFEATVGKD 185 IHLDTEVGHTGTGLSAESVVPTDPASESP--------ELKPGFEAAVQKD 188 VHFDADAGRSGVGLTAELAGPTKPTHEHAFDPTTESAELKPGFEAAVEKN 196 EQVQAQVDIKSAVPSAPSKASFGKVVIDKD-----------DLHQSATTD 183 ----------PVIDSASAEASFDEVSIEKQ-----------GPVQSATTD 159 ---QPSASTVNVQTKRFDERPVTPQTNDES----------VTKSHSMSRD 174 -----GSNSFPVTSKNLSDMEMDKEEDKDG------------VEKSLTAS 168 HQGDAETNENESEFYDQHDNGSRQPNDDGD--------VDTKPRNDNENE 180 -----TSEDTPVIPKLVSVADMNTNESIS--------------KEVPIAD 166

EWG40990.1 KLP03525.1 EWZ36701.1 ESU17197.1 KPA39364.1 XP_003053980.1 XP_013952680.1

210 220 230 240 250 ....|....|....|....|....|....|....|....|....|....| VGEFSSSDNAAMKDASSSSVGTVFTEPIFTDSLVAEPASTLADEWTANAG 235 FGDFSSSDNAAMKDASSSLVG-----PVFTDSFVAEPASSLVDEWT---- 229 FSGFSSSEHAALKDASSSPVE-----PIFTDSLVAEPASSPADEWT---- 237 ASEHSEADSASAVDPWT-------GAPVIAEPVTAEPAGFDSPSVK---- 222 VFEHNEADSASAVDAWT-------SAPVSVNTDITEIADFDSPSDK---- 198 SVDKGWTSQAATNNSSKS-------------KLSRKSSASPTGIAS---- 207 SGEDAISDNVSEASSNAT----------------HGSNGSLNSNAS---- 198

Ac KFH42786.1 Th KKO99655.1

TSEPPKEESPRTYDPVKG--------ELRTDDENKASETTNHNKCP---- 218 VHEKVADDNVSEASSNAT----------------HGSNGSLASNLS---- 196

Fv Ff Fo Fg Fl Nh Tv Ac Th

EWG40990.1 KLP03525.1 EWZ36701.1 ESU17197.1 KPA39364.1 XP_003053980.1 XP_013952680.1 KFH42786.1 KKO99655.1

260 270 280 290 300 ....|....|....|....|....|....|....|....|....|....| TVNTADEWNTNTAPTGEQDGNANNHASDSGAKNILDPDEVGISTSRWAPG 285 ---------TNTAPGGGHGDNTENHSSDSGVKDILDPEEVGISTSRWAPG 270 ---------TNTAPTGEQRDNADNHGFDSGVKNILDPDEVGISTSRWAPG 278 ---------DDVTTVIAND--------NDGGTSVKTGSDAGKGGHNGLEG 255 ---------DHASIVGDGNDDVTTGFINDGDASVKAGSDTDKGVQNGLEG 239 ---------VDSAVGLGSSRWAPKNLEEAAARSVKNQSPPTQKRPSPLQK 248 ---------QESKKKDTID--LGRKVGLQASIWANAPDKSPKGIKHTSSP 237 ---------QNNNVVNGDDGIEDASDEVEAASGAEDSPKTSDSASRPMTG 259 ---------QESSKKKNEDSGLGRKVGLQASIWANAPAKTPERKKHNGSP 237

EWG40990.1 KLP03525.1 EWZ36701.1 ESU17197.1 KPA39364.1 XP_003053980.1 XP_013952680.1 KFH42786.1 KKO99655.1

310 320 330 340 350 ....|....|....|....|....|....|....|....|....|....| NSPT-ASKPTNPAVDFKEPESGEEHNQAQEQAQTPVVAPSRRAVNFSYNP 334 NSPTNSTKPTKPAVDFKDNESGEER----EKVQTPVVAVSRRAVNFSYNP 316 NSPSNSSQPAELAFEVRDNEKGQEQ------EQTPVVAVSRRAVNFSYDP 322 DVDTKLGHDAPKSID-------------------GATPVSRRAVNFAFSA 286 ----------------------------------GTTLVTRRAVNFSYSA 255 QSSP----------------------------PSQKRIVPAAAPVFVADP 270 QVQS-----------------------------PVKRQKAATPQG----- 253 IEASQWAPRNEAPNAD---------------LHVQAPADSTPPSPAPKAI 294 KSQS-----------------------------PVKRQKPVVPQGRKPAV 258

EWG40990.1 KLP03525.1 EWZ36701.1 ESU17197.1 KPA39364.1 XP_003053980.1 XP_013952680.1 KFH42786.1 KKO99655.1

360 370 ....|....|....|....|....|. SNAEKLMETAEIFANLKKAFLMGKADNAEKLLETAEMFANVKKAFLMGKANNAEKLLETAEKFANVK-AFLMAKAEAADKLRDTTAKFASLKSSFLMGKPI EAADKLRDATSKFASLKSGFLMGKAT GNASKVRHATKKMANMKAFLSGTPMF ---PKTRMNLPQIAAAKNFLREGSAAASRPPTLTAEKIASMKEFLRMN--ALVKKTKFNLPQIAAAKSFLKATMV-

Fv Ff Fo Fg Fl Nh Tv Ac Th

Fv Ff Fo Fg Fl Nh Tv Ac Th

359 341 346 312 281 296 275 317 283

Figure S4 Sequence alignment of putative Fveg_03194 homologs A BLASTP search of NCBI’s non-redundant protein database was performed with the sequence of F. verticillioides Fveg_03194. The sequences of eight putative homologs were downloaded, imported into Bioedit 7.2.5, and aligned with Clustal W. Columns were shaded according to the BLOSUM62 similarity matrix. Each sequence is specified by its accession number: EWG40990.1 (Fveg_03194), F. verticillioides; KLP03525.1, F. fujikuroi; EWZ36701.1, F. oxysporum; ESU17197.1, F. graminearum; KPA39364.1, F. langsethiae; XP_003053980.1, Nectria haematococca; XP_013952680.1, Trichoderma virens; KFH42786.1, Acremonium chrysogenum; KKO99655.1, Trichoderma harzianum.