FSH-induced ovulation in intact and ... - Reproduction
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FSH-induced ovulation in intact and mice V.
Montgomery,
D.
Loutradis,
D.
hypophysectomized
Tulchinsky
and A.
Kiessling
t Department of Obstetrics, Gynecology, and Reproductive Biology, and the %Laboratory of Human Reproduction and Reproductive Biology, Harvard Medical School, Brigham & Women's Hospital, Boston, MA 02115, U.S.A.
Summary. A single, ovulatory dose of 25 \g=m\gof a highly purified preparation of ovine FSH caused ovulation in 89% of hypophysectomized and 91% of intact female mice primed 48 h earlier with PMSG; the number of oocytes recovered (29\m=.\4\m=+-\4\m=.\7and 22 \m=+-\2\m=.\/mouse ovulating, respectively) compared favourably with the 20\m=.\0\m=+-\2\m=.\9 oocytes per ovulating female recovered from animals that received PMSG + hCG. After oFSH injection, 82% of oocytes released were fertilized and developed to blastocysts. That the trace contamination (
