Function Vip-F Vip-R Vip-flag-F Vip-flag-R Vip-RFP-Up-F V - PLOS

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SRi2-Up-F. SRi2-Up-R. SRi2-Do-F. SRi2-Do-R. pAc -SR-C-F. pAc -SR-C-R. pAc-Sf-S2-F. pAc-Sf-S2-R. SR-C-N-F. SR-C-N-R. SR-F-1-F. SR-F-1-R. SR-F-2-F.
S1 Table: Primers used in this study Primer name Sequence(5→3) Vip-F TACCATGGTAATGAACAAGAATAATACTAAATTAAGCAC Vip-R TTCTCGAGCTTAATAGAGACATCGTAAAAATGTAC Vip-flag-F ACTTTAAGAAGGAGATATACCATGGTAATGAACAAGAATAATACT AAATTAAGC Vip-flag-R GTGGTGGTGGTGGTGGTGCTTATCGTCGTCATCCTTGTAATCCTTA ATAGAGACATCGTAAAAATGTAC Vip-RFP-Up-F ACTTTAAGAAGGAGATATACATGAACAAGAATAATACTAAATTAAGC Vip-RFP-Up-R AGGCCTGCATCTTAATAGAGACATCGTAAAAATG Vip-RFP-Do-F CTCTATTAAGATGCAGGCCTCTGCAGTCGACGGGCC Vip-RFP-Do-R GTGGTGGTGGTGGTGGTGCACACTAGTCTTGTACAGCTCGTCCATGC RFP-F CAAGAGCTCATGCAGGCCTCTGCAGTCGACGGGC RFP-R CGAAAGCTTCTAGATCCAATGACTCTAGATTCG Sf-CCP-F ACACCATGGCATACCCGGCTTCAACATGTCCGAT Sf-CCP-R ACACTCGAGGCAGCCCGATTTGACACAAACGGGT Dm-CCP-F GCGCCATGGCACGATGTGAAAGATCTATAGATTTGG Dm-CCP-R ACACTCGAGGGGCCTCGCACATCCCCTACTG pIZT-SR-C-F TCGAATTTAAAGCTTGGTACAATGGTAATGTTTATTAAGTGTTTATTATT pIZT-SR-C-R AGGCTTACCTTCGAACCGCGGTTCATCATCAACATCGGACTTGA SRi1-Up-F TCGAATTTAAAGCTTGGTACATGGGTAGTGTTTTTCTGTTTACCC SRi1-Up-R CACATGTATGGAAGGTGGTCCCCCCACTGCTCAAATAGG SRi1-Do-F CAGTGGGGGGACCACCTTCCATACATGTGGTAGTAAAAG SRi1-Do-R AATGGTGATGGTGATGATGAATGGGTAGTGTTTTTCTGTTTACCC SRi2-Up-F TCGAATTTAAAGCTTGGTACTGCTGTCCAGACTTCCTGGACG SRi2-Up-R CTAAACCTACACCTATACTGCAGACACTTCAATGGGCAT SRi2-Do-F AAGTGTCTGCAGTATAGGTGTAGGTTTAGGAGTAGGCTT SRi2-Do-R AATGGTGATGGTGATGATGATGCTGTCCAGACTTCCTGGACGTCT pAc -SR-C-F AGACCCCGGATCGGGGTACAATGGTAATGTTTATTAAGTGTTTATTATT pAc -SR-C-R AGGCTTACCTTCGAACCGCGGTTCATCATCAACATCGGACTTGA pAc-Sf-S2-F AGACCCCGGATCGGGGTACAATGGCGGACGCAGCTCCAGCCGG pAc-Sf-S2-R AGGCTTACCTTCGAACCGCGGAGCCTTGAACTCTGAGTAAGGGGAC SR-C-N-F CTGTTCCAGGGGCCCCTGGGATACTACCCGGCTTCAACATGTCCG SR-C-N-R TCAGTCACGATGCGGCCGCTCCTATCCACTCAGACCTCCAGATTTCTTC SR-F-1-F CTGTTCCAGGGGCCCCTGGGATACCCGGCTTCAACATGTCCG SR-F-1-R TCAGTCACGATGCGGCCGCTCCTATGACACTTTTGCTGAACTATCACG SR-F-2-F CTGTTCCAGGGGCCCCTGGGAACGGAATGTGACTTCGAGCTGCCGG SR-F-2-R TCAGTCACGATGCGGCCGCTCCTAGAGCATGGCTTTGGTGCAGTTCTCC SR-F-3-F CTGTTCCAGGGGCCCCTGGGAGAAGCCACCACGCCACCAGCTGTT SR-F-3-R TCAGTCACGATGCGGCCGCTCCTATAATGTAGCTCCACTCAGACCTCC Sf-actin-RT-F TCCTCCGTCTGGACTTGGC Sf-actin-RT-R CTTCTCCTTGATGTCACGAACG Sf-SR-RT-F ATGCTCGAAGCCACCACG Sf-SR-RT-R CGACGCAGACAATGCTACAAC

Function Vip cloning Vip cloning Vip-flag cloning Vip-flag cloning Vip-RFP cloning Vip-RFP cloning Vip-RFP cloning Vip-RFP cloning RFP cloning RFP cloning Sf-CCP cloning Sf-CCP cloning Dm-CCP cloning Dm-CCP cloning Sf-SR-C cloning Sf-SR-C cloning SR-C RNAi SR-C RNAi SR-C RNAi SR-C RNAi SR-C RNAi SR-C RNAi SR-C RNAi SR-C RNAi SR-C cloning SR-C cloning Sf-S2 cloning Sf-S2 cloning SR-C-N cloning SR-C-N cloning SR-F-1 cloning SR-F-1 cloning SR-F-2 cloning SR-F-2 cloning SR-F-3 cloning SR-F-3 cloning Actin qRT-PCR Actin qRT-PCR Sf-SR-C qRT-PCR Sf-SR-C qRT-PCR

pET-SRi-Up-F pET-SRi-Up-R pET-SRi-Do-F pET-SRi-Do-R Hypi-Up-F Hypi-Up-R Hypi-Do-F Hypi-Do-R Se-actin-RT-F Se-actin-RT-R Se-SR-RT-F Se-SR-RT-R pJF-Sf-SR-C-F pJF-Sf-SR-C-R

AGCGGATAACAATTCCCCTCCTCGTTATACCCTGGTTGG CAGATCGTAAATAGAGGTTGCAGCGTCCAACAC CAACCTCTATTTACGATCTGGAAATTATCACCG AGTGGTGGTGGTGGTGGTGCCTCCTCGTTATACCCTGGTTGG AGCGGATAACAATTCCCCTAAGGTGCTCGGAGAAGGAGAG CACAGCAACGAGGAGCACGCCGAACGACCAG GCGTGCTCCTCGTTGCTGTGGACATCCTTAGCC AGTGGTGGTGGTGGTGGTGCAAGGTGCTCGGAGAAGGAGAG ATCCTCCGTCTGGACTTGGC CTTCTCCTTGATGTCACGCACG AACCTATAACAGTGTCCGTTGAAG AATGTTGCTCCACTCAGTCCTC ATCCTTTACTTCAGGCGGCCGCGGCTCGAGCAAAATGTTTATTAA GTGTTTATTATTTTTATTTG TCCTTCACAAAGATCCTCTAGATTATTCATCATCAACATCGGAC

Se-SR-C RNAi Se-SR-C RNAi Se-SR-C RNAi Se-SR-C RNAi pET-Hypi pET-Hypi pET-Hypi pET-Hypi Actin qRT-PCR Actin qRT-PCR Se-SR-C qRT-PCR Se-SR-C qRT-PCR Sf-SR-C cloning Sf-SR-C cloning