Genetic control of maize shoot apical meristem architecture Addie M. Thompson*, James Crants*, Patrick S. Schnable§, Jianming Yu†, Marja C. P. Timmermans‡, Nathan M. Springer**, Michael J. Scanlon§§, Gary J. Muehlbauer*, ** *Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55108 §Department of Genetics, Development and Cell Biology, and Agronomy, Iowa State University, Ames, Iowa 50011 †Department of Agronomy, Iowa State University, Ames, Iowa 50011. ‡Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724. **Department of Plant Biology, University of Minnesota, Saint Paul, Minnesota 55108. §§Department of Plant Biology, Cornell University, Ithaca, New York 14853.
Corresponding author: Gary J. Muehlbauer Plant Biology Room 250 Biological Sciences 1445 Gortner Avenue Saint Paul, MN 55108 612‐624‐2755
[email protected]
DOI: 10.1534/g3.114.011940
0 1 2 3 4
B
10 12 14 16 18
M
B
120 140 160 180 200 220
0 .01 .02 .03 .04 .05
Density 0 .01 .02 .03
Midpoint Width
BM 90 100 110 120 130
Volume
M
B
1e+6 2e+6 3e+6 4e+6 5e+6
0.6 0.8 1.0 1.2 1.4
Arc Length
M
B
0 .02 .04 .06 .08
Density 0 .01 .02 .03
M
0 2e‐7 4e‐7 6e‐7 8e‐7
Height:Width Ratio
Arc Cell Count
P2‐P1 Internode Length
M B 50 60 70 80 90
Figure S1 Density distributions of remaining SAM traits in the IBMRIL population. B73 and Mo17 are indicated as B and M, respectively.
2 SI
A. M. Thompson et al.
A
Gene GRMZM2G341089 GRMZM2G003640 GRMZM2G053027 GRMZM2G164413 GRMZM2G179662 GRMZM2G150471 GRMZM2G042548 GRMZM2G045404 GRMZM2G045366 GRMZM2G469142 GRMZM2G051894 GRMZM2G088427 GRMZM2G055898 GRMZM2G180435 GRMZM2G352695 GRMZM2G123277 GRMZM2G318635 GRMZM2G177929 GRMZM2G459110 GRMZM2G056303 GRMZM2G164405 GRMZM2G177970 GRMZM2G399921 GRMZM2G175728 GRMZM2G178996 GRMZM2G021704 GRMZM2G176699 GRMZM2G024211 GRMZM2G179658 GRMZM2G127431 GRMZM2G164428 GRMZM2G042099 GRMZM2G128688 GRMZM2G106881 GRMZM2G382341 GRMZM2G163761 GRMZM2G531719 GRMZM2G158568 GRMZM2G069594 GRMZM2G018103 GRMZM2G323622
B
Gene GRMZM2G386643 GRMZM2G118098 GRMZM2G119906 GRMZM2G150098 GRMZM2G154437 GRMZM2G165383 GRMZM2G165461 GRMZM2G166345 GRMZM2G060167 GRMZM2G130425 GRMZM2G002548 GRMZM2G064212 GRMZM2G382104 GRMZM2G049536 GRMZM2G073826 GRMZM2G111529 GRMZM2G061876 GRMZM2G140582 GRMZM2G159849 GRMZM2G018403 GRMZM2G165535 GRMZM2G009785 GRMZM2G416216 GRMZM2G057753 GRMZM2G095082 GRMZM2G457621 GRMZM2G006565 GRMZM2G311919 GRMZM2G119725 GRMZM2G062199 GRMZM2G119950 GRMZM2G114675 GRMZM2G148404
C
Gene GRMZM2G 118385 GRMZM2G 077079 GRMZM2G460078 GRMZM2G011479 GRMZM2G049888 GRMZM2G149788 GRMZM2G377589 GRMZM2G042412 GRMZM2G134857
0 100 200 RPM Value
Figure S2 Patterns of expression in candidate genes. Gene expression across SAM ontogeny for genes expressed in the B73 SAM and IBMRIL apices located under QTL with expression significantly correlated with SAM architecture (see Figure 5) for SAM height (A), width (B), and PIL (C). PS=Proembryo stage SAM, TS=Transition stage SAM, CS=Coleoptile stage SAM, L1=L1 SAM of 14‐day seedling, SAM=SAM of 14‐day seedling, LM=Lateral Meristem of 14‐day seedling. Annotations, locations, and RPM values of genes are listed in Table S4.
A. M. Thompson et al.
3 SI
Tables S1‐S7 Available for download at http://www.g3journal.org/lookup/suppl/doi:10.1534/g3.114.011940/‐/DC1 Table S1 Genotypes used for IBM subset and NILs. Table S2 SAM measurements for time course experiment Table S3 Correlation values for SAM traits. Table S4 SAM measurements for IBM RILs examined Table S5 All QTL for SAM traits. Table S6 SAM data for NILs examined Table S7 Annotations, locations, and RPM values of genes in Figure S2.
4 SI
A. M. Thompson et al.
