Genome-wide expression patterns associated with ... - CiteSeerX
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Genome-wide expression patterns associated with ... - CiteSeerX
Dec 12, 2009 - Abstract. Background: The molecular mechanisms of CC (cholangiocarcinoma) oncogenesis and progression are poorly understood.
Seol et al. BMC Cancer 2011, 11:78 http://www.biomedcentral.com/1471-2407/11/78
RESEARCH ARTICLE
Open Access
Genome-wide expression patterns associated with oncogenesis and sarcomatous transdifferentation of cholangiocarcinoma Min-A Seol1†, In-Sun Chu3†, Mi-Jin Lee1, Goung-Ran Yu1, Xiang-Dan Cui1, Baik-Hwan Cho2, Eun-Kyung Ahn4, Sun-Hee Leem4, In-Hee Kim, Dae-Ghon Kim1*
Abstract Background: The molecular mechanisms of CC (cholangiocarcinoma) oncogenesis and progression are poorly understood. This study aimed to determine the genome-wide expression of genes related to CC oncogenesis and sarcomatous transdifferentiation. Methods: Genes that were differentially expressed between CC cell lines or tissues and cultured normal biliary epithelial (NBE) cells were identified using DNA microarray technology. Expressions were validated in human CC tissues and cells. Results: Using unsupervised hierarchical clustering analysis of the cell line and tissue samples, we identified a set of 342 commonly regulated (>2-fold change) genes. Of these, 53, including tumor-related genes, were upregulated, and 289, including tumor suppressor genes, were downregulated (4fold change), and 267 differentially downregulated genes (four-fold change), and 267 downregulated genes (
