GENOTYPES OF JC VIRUS IN RENAL TRANSPLANT RECIPIENTS IN ISFAHAN, IRAN Sayyedeh Rahmaneh Atyabi1 , Majid Bouzari2 1- Master of Microbiology, Isfahan University, Isfahan, Iran
[email protected] 2- Professor of Virology. Dept. Biology, Faculty of Science, Isfahan University, Isfahan, Iran INTRODUCTION JC virus (JCV) is an opportunistic infection in childhood and remains latent in bone marrow, renal epithelial cells and other tissues
asymptomatically and then in immunosuppressed individuals such as rheumatoid arthritis patients, transplant recipients that undergo immunosuppressive treatment, hematological malignancies and patients infected with human immunodeficiency virus can lead to a clinical disorder named as progressive multifocal leukoencephalopathy (PML) (Figure 1). This disorder causes demyelination of the white matter of brain. Relationship between JCV and human brain tumors and cancers such as medulloblastomas, neuroectodermal, astrocytomas, glioblastoma and neuroblastomas has already been reported. More than ten genotypes have been detected for the virus and seven types are more important. In immunosuppressed individuals an aggressive form of the virus specially genotype 2B is shed from
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METHODS The presence of JCV DNA in urine of 100 patients with kidney transplant and 100 healthy individuals were tested. PCR with specific primers was performed on urine pellet for detection of VP1 gene (Figure 2). To determine the genotypes of the virus, sixteen and seven positive PCR products from transplant recipients and healthy individuals were sequenced, respectively. Fisher's exact test was used for statistical analyses. Phylogenetic tree was constructed by MEGA 6 (Figure 3).
Results JCV DNA was detected in 43.82%, and 7% of kidney transplant recipients and healthy individuals respectively. Genotypes 1 and 3 were found in 1(6.25%) and 15(93.75%) kidney transplant recipients respectively, while Genotype 1 was found in 2(28.57%) and genotype 3 was found in 5(71.42%) of the healthy individuals. The frequency of the infection was significant higher in kidney transplant recipients (P
