J Physiol Biochem DOI 10.1007/s13105-015-0441-9
ORIGINAL PAPER
Glucomannan and glucomannan plus spirulina added to pork significantly block dietary cholesterol effects on lipoproteinemia, arylesterase activity, and CYP7A1 expression in Zucker fa/fa rats Laura González-Torres & Miguel Vázquez-Velasco & Raúl Olivero-David & Sara Bastida & Juana Benedí & Rafaela Raposo González & Mª José González-Muñoz & Francisco J. Sánchez-Muniz
Received: 9 September 2015 / Accepted: 6 October 2015 # University of Navarra 2015
Abstract Zucker fa/fa rats easily develop dyslipidemia and obesity. Restructured pork (RP) is a suitable matrix for including functional ingredients. The effects of glucomannan- RP or glucomannan plus spirulinaenriched RP on plasma lipid/lipoprotein levels, cytochrome P450 7A1 (CYP7A1) expression, and arylesterase activity in growing fa/fa rats fed high-energy, high-fat cholesterol-enriched diets were tested. Groups of six rats each received diet containing 15 % control-RP (C), 15 % glucomannan-RP diet (G), 15 % Laura González-Torres and Miguel Vázquez-Velasco contributed equally to this work. L. González-Torres : M. Vázquez-Velasco : R. Olivero-David : S. Bastida : F. J. Sánchez-Muniz (*) Departamento de Nutrición y Bromatología I (Nutrición). Facultad de Farmacia, Universidad Complutense de Madrid, Plaza Ramón y Cajal s/n., 28040 Madrid, Spain e-mail:
[email protected] J. Benedí Departamento de Farmacología. Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain R. R. González Centro de Análisis Sanitarios Clínicos de la UCM. Facultad de Farmacia, Universidad Complutense de Madrid, 28040 Madrid, Spain M. J. González-Muñoz Departamento de Nutrición, Bromatología y Toxicología. Facultad de Farmacia, Universidad de Alcalá, 28871 Alcalá de Henares, Madrid, Spain
glucomannan + spirulina-RP diet (GS), and same diets enriched with 2.4 % cholesterol and 0.49 % cholic acid (cholesterol-enriched control (HC), cholesterol-enriched glucomannan (HG), and cholesterol-enriched glucomannan + spirulina (HGS) diets) over a 7-week period. C diet induced obesity, severe hyperglycemia, moderate hypercholesterolemia, and hypertriglyceridemia. Those facts were not significantly modified by G or GS diets. G diet increased CYP7A1 expression but decreased the total cholesterol/high density lipoproteins (HDL)-cholesterol ratio (p
