Glucose-induced post-translational activation of

Supplementary table 2 List of plasmids used in this study Source/refere Plasmid

Characteristics/expression product nce

pYX142 (no insert)

CEN LEU2 TPI promoter

pYX142 (HA-Pph21)

CEN LEU2 TPI promoter, HA-tagged Pph21

L369A

pYX142 (HA-Pph21

R&D Systems [3]

L369A

)

CEN LEU2 TPI promoter, HA-tagged Pph21

This study*

pNC160 (HA-Glc7)

CEN TRP1 CUP1 promoter, HA-tagged Glc7

[9]

pKD89/pRS316 (HA- Reg1)

CEN URA3 REG1 promoter, HA-tagged Reg1

[10]

pKD104/pRS316 (HA- Reg1∆8)

CEN URA3 REG1 promoter, HA-tagged Reg1∆8

pKD114/pRS316 (HA- Reg1 pRS316 (Snf1) pRS316 (Snf1

I466M F468A

)

CEN URA3 REG1 promoter, HA-tagged Reg1

I466M F468A

CEN URA3 SNF1 promoter, HA-tagged Snf1

T210A

)

CEN URA3 SNF1 promoter, HA-tagged Snf1

[10] [10] [11]

T210A

[11]

* Mutagenesis (GeneEditor in vitro Site-Directed Mutagenesis System Kit, Promega) on pHA-Pph21 was performed using the following primers: Fw 5’-GCAGAAAGACGCCAGATTACTTTGCATGAGTATGTATACATATATGCATC-3’ Rev 5’-GATGCATATATGTATACATACTCATGCAAAGTAATCTGGCGTCTTTCTGC-3’ Substituted residues leading to Leu369Ala mutation are indicated in bold.