... and abscission (Hamilton et al., 1990; Oeller et al., 1991; John et al., 1995). As a ..... John I, Drake R, Farrell A, Cooper W, Lee P, Horton P,. Grierson D. 1995.
nase has been cloned (Smith et al., 1993) and its physiolog- ..... John. Wiley & Sons, New York, pp 4.3.14.3.4. Baysdorfer C, Kremer DF, Sicher RC (1989) Partial purification .... icantly enhances the yield of high-quality, RNA from cotton.
PCR cDNA synthesis kit (CLONTECH Laboratories, Inc., Palo Alto, CA) to produce two .... tems) linked to a MacIntosh G4 (Apple Computer Inc., PE Applied.
Jun 2, 2010 - The authors propose a new test, the 'half Student's t-test', specif- .... Assuming normality, tp is a Student's t distribution ..... In light of these,.
Oct 9, 2017 - Bagby, R. M., Parker, J. D. A., and Taylor, G. J. (1994a). ... G. J. Taylor, R. M. Bagby, and J. D. A. Parker (Cambridge: Cambridge University.
Monday, 20 July 2009; 7:30 am - 10:00 am Poster Session B
Glutaredoxin Pathway Genes Are Differentially Expressed in Mature Porcine Oocytes with Varying Developmental Potentials. Ye Yuan 1 and Rebecca L. Krisher 2 1 University of Illinois, Urbana, IL, USA; 2 $
ABSTRACT 370 Oocyte competence is a key factor limiting female fertility.Poor oocyte competence contributes to failed fertilization,compromised embryonic and fetal development, and even affectsoffspring health. In pigs, oocyte competence is progressivelyobtained as females approach puberty and undergo their firstseveral estrus cycles. The poor quality of oocytes derived fromprepubertal females, compared to adults, suggests that essentialprocesses of cytoplasmic maturation have not been fulfilled.The glutaredoxin pathway plays an important role in cellularredox regulation and defends the cell against oxidative stressand apoptosis. The objective of this study was to examine therole of glutaredoxin pathway related genes in oocyte competence,by comparing gene expression in metaphase II oocytes derivedfrom prepubertal (poor developmental competence) and adult (improveddevelopmental competence) pigs. Cumulus-oocyte complexes collectedfrom 2-6mm follicles from prepubertal or adult porcine ovarieswere matured in vitro in defined Purdue Porcine Medium (containing2mM glucose, 6mM lactate, 0.5mM cysteamine, 50ng/ml EGF, 0.01units/mlLH, 0.01units/ml FSH and supplemented with 1% recombumin (Vitrolife,Kungsbacka, Sweden) and 0.2% fetuin) in 5% CO 2 in air at 39°C for 44h. Oocytes with a visible polar body were frozenat -80 °C in pools of 20. mRNA from 3 pools of oocytes inboth age groups (prepubertal and adult) were used to quantifythe relative expression of 16 glutaredoxin pathway related genesby reverse transcription followed by linear amplification usingthe MEGAscript High Yield Transcription Kit (Ambion), and real-timePCR was performed in duplicate. Relative quantification of thetranscripts was analyzed by REST 2005 version 1.9.12 software(Corbett Research); GAPDH expression was used as a reference.Analysis of gene expression showed that glutaredoxin 2 (GLRX2), protein disulfide isomerase 6 (PDIA6 ), thioredoxin reductase1(TRXR1 ) and tumor necrosis factor-alpha (TNF-alpha ) weremore highly expressed in prepubertal derived oocytes (P
