Hemoparasite and hematological parameters in Nile tilapia
Karen Giuliana Lourenço, Gustavo Silva Claudiano, Silas Fernandes Eto, Jefferson Yunis Aguinaga, Paulo Fernandes Marcusso, et al. Comparative Clinical Pathology ISSN 1618-5641 Comp Clin Pathol DOI 10.1007/s00580-012-1638-8
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Author's personal copy Comp Clin Pathol DOI 10.1007/s00580-012-1638-8
ORIGINAL ARTICLE
Hemoparasite and hematological parameters in Nile tilapia Karen Giuliana Lourenço & Gustavo Silva Claudiano & Silas Fernandes Eto & Jefferson Yunis Aguinaga & Paulo Fernandes Marcusso & Rogério Salvador & Julieta Rodini Engrácia de Moraes & Flávio Ruas de Moraes
Received: 22 August 2012 / Accepted: 16 October 2012 # Springer-Verlag London 2012
Abstract The accelerated growth of finfish aquaculture has resulted in a series of health problems, including blood disorders by hemoparasites; there are scarce studies about these agents and their impact in actual intensive farming. The aim of this study was to identify and quantify the hemoparasites present in monocytes and erythrocytes in the blood of tilapia and their correlation with the hematological profile. Blood samples were collected from caudal vessels of 15 cage-reared Nile tilapia (Oreochromis niloticus), with 70±10 g weight on average, from Itambaracá Municipality, Parana State, Brazil. The total red blood cells, mean corpuscular volume, hematocrit, total and differential white blood cells counts, and the number of thrombocytes were determined in blood smears stained with MayGrünwald–Giemsa–Wright and Quick Panoptic. The results showed the presence of pleomorphic cytoplasmic inclusions with corrugated appearance and basophilic staining, mainly in monocytes, suggesting Anaplasmataceae parasitemia and inclusions with the same morphological characteristics in erythrocytes of one Nile tilapia. The hematological analysis K. G. Lourenço : S. F. Eto : P. F. Marcusso : R. Salvador Department of Animal Pathology, State University of Northern Paraná-Uenp, Campus Luiz Meneghel, Bandeirantes, Paraná, Brazil G. S. Claudiano : J. R. E. de Moraes : F. R. de Moraes (*) Department of Animal Pathology, College of Agricultural Sciences and Veterinary, UNESP University Estadual Paulista, Jaboticabal, São Paulo, Brazil e-mail:
[email protected] J. Y. Aguinaga Aquaculture Center of UNESP, Campus Jaboticabal, Jaboticabal, São Paulo, Brazil
showed no significant difference (P
