Hepatitis E Epidemic, Biratnagar, Nepal, 2014 - CiteSeerX

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false-positive results or asymptomatic infections. However, our findings of elevated levels of subtype H7N9 antibody among 6.7% of contacts during this epidemic in China offer evidence that human-to-human transmission of H7N9 virus may occur among contacts of infected persons. Acknowledgments We thank all subjects for participating in the study, the staff of the Wuxi Center for Disease Control and Prevention for collecting samples, and Yuelong Shu and Tian Bai for providing influenza subtype H7N9 and H5N1 virus samples. This work was supported by the grants from the Program of International Science and Technology Cooperation (2013DFA30800) of the Ministry of Science and Technology of China; the National Natural Science Foundation of China (no. 81402730); the Key Project of Jiangsu Provincial Department of Health (H201448); and the Major Project of Wuxi Bureau of Health (G201201, Z201404). References

1. Gao R, Cao B, Hu Y, Feng Z, Wang D, Hu W, et al. Human infection with a novel avian-origin influenza A (H7N9) virus. N Engl J Med. 2013;368:1888–97. http://dx.doi.org/10.1056/ NEJMoa1304459 2. Li Q, Zhou L, Zhou M, Chen Z, Li F, Wu H, et al. Epidemiology of human infections with avian influenza A(H7N9) virus in China. N Engl J Med. 2014;370:520–32. http://dx.doi.org/10.1056/ NEJMoa1304617 3. Qi X, Qian YH, Bao CJ, Guo XL, Cui LB, Tang FY, et al. Probable person to person transmission of novel avian influenza A (H7N9) virus in Eastern China, 2013: epidemiological investigation. BMJ. 2013;347:f4752. http://dx.doi.org/10.1136/bmj.f4752 4. Shanghai Health and Family Planning Commission of China. A health care worker was infected with H7N9 avian influenza virus [in Chinese] [cited 2014 Jan 20]. http://www.wsjsw.gov.cn/wsj/ n422/n422/u1ai132455.html 5. China National Health and Family Planning Commission. Chinese guideline for prevention and control for human infection with A(H7N9) avian influenza; 2014 ed. [in Chinese] [cited 2014 Jan 27]. http://www.moh.gov.cn/jkj/s3577/201401/8c1828375a7949cd8 5454a76bb84f23a.shtml 6. Editorial Team Journal of Thoracic Disease. Guideline on prevention and control of H7N9 avian influenza human infection. J Thorac Dis. 2013;Suppl 2:S168–72. 7. World Health Organization. Real-time RT-PCR protocol for the detection of avian influenza A(H7N9) virus [cited 2013 Apr 15]. http://www.who.int/influenza/gisrs_laboratory/cnic_realtime_rt_ pcr_protocol_a_h7n9.pdf 8. World Health Organization. Serological detection of avian influenza A(H7N9) virus infections by modified horse red blood cells haemagglutination-inhibition assay [cited 2013 Dec 20]. http://www.who.int/influenza/gisrs_laboratory/cnic_serological_ diagnosis_hai_a_h7n9_20131220.pdf 9. Hsieh SM, Huang YS, Chang SY, Lin PH, Chang SC. Serological survey in close contacts with a confirmed case of H7N9 influenza in Taiwan. J Infect. 2013;67:494–5. http://dx.doi.org/10.1016/j. jinf.2013.08.003 10. Qiu C, Yuan S, Tian D, Yang Y, Zhang A, Chen Q, et al. Epidemiologic report and serologic findings for household contacts of three cases of influenza A (H7N9) virus infection. J Clin Virol. 2014;59:129–31. http://dx.doi.org/10.1016/j.jcv.2013.12.004

Address for correspondence: Wu-Chun Cao, State Key Laboratory of Pathogen and Biosecurity, Beijing Institute of Microbiology and Epidemiology, 20 Dong-Da St, Fengtai District, Beijing 100071, China; email: [email protected]

Hepatitis E Epidemic, Biratnagar, Nepal, 2014 Ananta Shrestha, Thupten K. Lama, Sneha Karki, Deepak R. Sigdel, Utsav Rai, Shyam K. Rauniyar, Mamun Al-Mahtab, Kazuaki Takahashi, Masahiro Arai, Sheikh M.F. Akbar, Shunji Mishiro Author affiliations: Liver Foundation Nepal, Kathmandu, Nepal (A. Shrestha); Civil Service Hospital, Kathmandu (T.K. Lama, S. Karki); Koshi Zonal Hospital, Biratnagar, Nepal (D.R. Sigdel, U. Rai, S.K. Rauniyar); Bangbandhu Sheikh Mujib Medical University, Dhaka, Bangladesh (M. Al-Mahtab); Toshiba General Hospital, Tokyo, Japan (K. Takahashi, M. Arai, S.M.F. Akbar, S. Mishiro) DOI: http://dx.doi.org/10.3201/eid2104.141512

To the Editor: We report a recent epidemic of hepatitis E in Biratnagar, Nepal. During the third week of April 2014, a total of 11 patients with acute jaundice came to hospitals in Biratnagar. IgM against hepatitis E virus (HEV) was detected in serum samples from all 11 patients. During the next 7 weeks, 1,861 patients with acute jaundice came to the outpatient departments of 2 of 5 large hospitals in Biratnagar; 123 patients were admitted to these 2 hospitals. Registries at these 2 hospitals indicated that 2 patients with acute jaundice came to these hospitals on April 14. On April 28; May 5, 12, 19, and 26; and June 2, 9, 16, 23, and 30, the number of patients with acute jaundice who came to these 2 hospitals were 42, 67, 58, 69, 48, 21, 5, 3, 1, and 0, respectively. Registries showed that this increased frequency of acute jaundice lasted until the end of May 2014, when it began to decrease and reached near zero by the first week of July. In addition, unusually large numbers of patients with acute jaundice came to 25 smaller private health care facilities in Biratnagar during April–May 2014. The Private and Boarding Schools’ Organization of Nepal closed 80 schools in Biratnagar and surrounding areas during the second week of May 2014 because of risk for disease transmission (1). The Biratnagar Zonal Health Authority and National Health Authority of Nepal issued special alerts by mass media regarding jaundice after the third week of April and advised using boiled water for consumption (2). Registries of major hospitals, smaller health clinics, and private physicians indicated that ≈7,000 patients were

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Figure. Phylogenetic analysis (UPGMA) of hepatitis E virus (HEV) sequences from the epidemic in Biratnagar, Nepal, 2014, on the basis of 412 nt within open reading frame 2 (DDBJ/EMBL/GenBank accession nos. AB98608–AB986107). All 40 HEV isolates from epidemic in Biratnagar segregated into a single, new cluster within genotype 1a. Values along branches indicate genotypes. Scale bar indicates nucleotide substitutions per site. 712

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affected during this epidemic. Fourteen patients died; these deaths occurred in Kathmandu, the capital of Nepal, or in different cities in India after these patients were transferred there for better treatment. Fifty pregnant women had acute jaundice, but none of these women died. The epidemic was presumed to be caused by consumption of contaminated water (3). In February and March 2014, water and sewerage pipelines were damaged in different areas of Biratnagar during construction and repair of roads. A survey conducted by the Department of Community Medicine, B.P. Koirala Institute of Health (Dharan, Nepal), found high levels of coliform bacteria in water supplies from different areas in Biratnagar during the epidemic. Tap water also looked cloudy and visibly contaminated (3). To obtain more information about the epidemic, the incidence of acute jaundice was determined for 656 prisoners and 75 security personnel at the Biratnagar Jail. The study protocol was approved by the Liver Foundation Nepal. Informed consent was not obtained because identity of patient samples remained anonymous. Acute jaundice was detected among 30 (4.6%) prisoners and 4 (5.3%) security personnel. The same source of consumable water was used by the general population, inmates, and security personnel in Biratnagar. To identify the causative agent of this epidemic, serum samples from 48 patients were obtained at Koshi Zonal Hospital, the largest government hospital in this zone. Hepatitis A virus RNA and IgM against hepatitis B virus core antigen was not detected in the 48 serum samples. Conversely, IgG, IgM, and IgA against HEV were detected in 47 (97.9%), 45 (94%), and 45 (94%) serum samples, respectively, and HEV RNA was detected in 42 (87.5%) of 48 serum samples, which indicated that the epidemic was caused by HEV. A partial 412-nt sequence from open reading frame 2 corresponding to nt 5944–6355 of the HEV B1 genome (4) was obtained as reported (5). We obtained 40 HEV isolates from the 42 samples and sequenced partial 412-nt segments. All 40 HEV sequences from the epidemic in Biratnagar segregated into a cluster within genotype 1a (Figure). These sequences showed 99.8% nt identity with each other but only 90.8%–95.4% nt identity with other HEV isolates from Nepal and those from India, Bangladesh, Pakistan, and China. Compared with previous HEV epidemics in Nepal (6) and other parts of the Indian subcontinent, the local government of Biratnagar and central government of Nepal took steps to contain the reported epidemic. Activities of public and private sectors in Biratnagar ended the epidemic in ≈12 weeks, and no new cases of acute jaundice have been reported in Biratnagar. Persons in Biratnagar were given information regarding epidemics and ways to contain them. They were

instructed by electronic media to use boiled water for consumption. It became clear that additional information regarding about maintaining water and sewerage systems during road construction and repair should also be provided. Because 14 patients died of HEV infection during this epidemic, more preparedness for epidemics of waterborne diseases is required to minimize unnecessary illnesses and deaths. References

1. Kantipur E. com. Jaundice outbreak: Biratnagar schools shut for 5 days [cited 2014 May 12]. http://www.ekantipur.com/2014/05/12/ top-story/jaundice-outbreak-biratnagar-schools-shut-for-5-days/ 389469.html 2. Ghimeri B. Alert issued against jaundice in Biratnagar [cited 2014 Apr 30]. http://www.ekantipur.com/2014/04/30/national/ alert-issued-against-jaundice-in-biratnagar/388967.html 3. Gautam M. Jaundice outbreak: experts blame contaminated water [cited 2014 May 12]. http://www.ekantipur.com/2014/05/12/ top-story/jaundice-outbreak-experts-blame-contaminated-water/ 389436.html 4. Tam AW, Smith MM, Guerra ME, Huang CC, Bradley DW, Fry KE, et al. Hepatitis E virus (HEV): molecular cloning and sequencing of the full-length viral genome. Virology. 1991; 185:120–31. http://dx.doi.org/10.1016/0042-6822(91)90760-9 5. Takahashi K, Okamoto H, Abe N, Kawakami M, Matsuda H, Mochida S, et al. Virulent strain of hepatitis E virus genotype 3, Japan. Emerg Infect Dis. 2009;15:704–9. http://dx.doi.org/ 10.3201/eid1505.081100 6. Shrestha SM. Hepatitis E in Nepal. Kathmandu Univ Med J (KUMJ). 2006;4:530–44.

Address for correspondence: Sheikh M.F. Akbar, Department of Medical Sciences, Toshiba General Hospital, 6-3-22 Higashi Oi, Shinagawa-Ku, Tokyo 140-8522, Japan; email: [email protected]

Human Parvovirus 4 Infection among Mothers and Children in South Africa Philippa C. Matthews, Colin P. Sharp, Amna Malik, William F. Gregory, Emily Adland, Pieter Jooste, Philip J. R. Goulder, Peter Simmonds, Paul Klenerman Author affiliations: University of Oxford, Oxford, UK (P.C. Matthews, A. Malik, E. Adland, P.J.R. Goulder, P. Klenerman); Oxford University Hospitals, Oxford (P.C. Matthews, P. Klenerman); The University of Edinburgh, Midlothian, Scotland, UK (C.P. Sharp, W.F. Gregory, P. Simmonds); University of Free State, Kimberley, South Africa (P. Jooste); NIHR Biomedical Research Centre, Oxford (P. Klenerman) DOI: http://dx.doi.org/10.3201/eid2104.141545

To the Editor: Human parvovirus 4 (PARV4) is a single-stranded DNA virus in the family Parvoviridae (1).

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