High-Throughput Quantitative Profiling of Serum N ... - CiteSeerX
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High-Throughput Quantitative Profiling of Serum N ... - CiteSeerX
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Proteomics and Protein Markers
High-Throughput Quantitative Profiling of Serum N-Glycome by MALDI-TOF Mass Spectrometry and N-Glycomic Fingerprint of Liver Fibrosis Richard K.T. Kam,1,2 Terence C.W. Poon,1,2,3* Henry L.Y. Chan,2,3 Nathalie Wong,4 Alex Y. Hui,2 and Joseph J.Y. Sung2,3
Background: The use of MALDI-TOF mass spectrometry (MS) in quantitative glycan profiling has not been reported. In this study, we attempted to establish a high-throughput quantitative assay for profiling serum N-glycome, and we applied the new assay to identifying serum N-glycans for diagnosis of liver fibrosis and cirrhosis. Methods: N-glycans from whole serum proteins in 2 L serum were released by enzymatic digestion, cleaned up by hydrophilic chromatography, and subsequently quantitatively profiled with a linear MALDI-TOF MS system, which was originally designed for quantitative proteomic profiling. Serum N-glycome profiles from 46 patients with chronic hepatitis B infection and with different degrees of liver fibrosis were examined. Results: The intra- and interassay CVs of peak intensities of the standard N-glycans were
