levigated alumina (Norton Co., Worcester, Mass.). The supernatant liquid obtained by centrif- ugation at 100,000 X g for 120 min was extracted with phenol.
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VOL. 55, 1966
Venable, J. H., and R. Coggeshall, J. Cell Biol., 25, 407 (1965). Caro, L. G., and R. P. van Tuibergeii, J. Cell Biol., 15, 173 (1962). 10 Other possible sources are the cell membrane and clusters of small vesicles occasionally found among the RE'R cisternae. 1 Gireene, L. J., C. H. W. Hirs, and C:. E. Palade, J. Biol. Chem., 238, 2054 (1963). 12 Siekevitz, P., and G. E. Palade, J. Biochem. Biophys. Cytol., 4, 557 (1958). 13 Transfer of secretory proteins into the cisternal space has been recently demonstrated by Redman et al.2 14 Warshawsky, H., C. P. Leblond, and B. Droz, J. Cell Biol., 16, 1 (1963). '5van Heyningen, H. E., Anat. Rec., 148, 485 (1964). 16 Laird, A. K., and A. D. Barton, Biochim. Biophys. Acta, 27, 12 (1958). 17 Morris, A. J., and S. R. Dickman, J. Biol. Chem., 235, 1404 (1960). 18 Redman, C. M., and L. E. Hokin, J. Biophys. Biochem. Cytol., 6, 207 (1959). 19 Sj6strand, F. S., in Ciba Foundation Symposium on the Exocrine Pancreas, ed. A. V. S. de Reuck, and M. P. Cameron (London: Churchill, 1962), p. 1. 20 Palade, G. E., and R. R. Bruns, in Small Blood Vessel Involvement in Diabetes Mellitus, ed. M. D. Siperstein, A. R. Colwell, Sr., and K. Meyer (Washington, D. C.: American Inst. Biol. Sciences, 1964), p. 39. 21 Karnovsky, M. J., J. Cell Biol., 27, 49A (1965). 8
9
LINCOMYCIN, AN INHIBITOR OF AMINOACYL SRNA BINDING TO RIBOSOMES* BY F. N. CHANG, C. J. SIH, AND B. WEISBLUM DEPARTMENT OF PHARMACOLOGY, SCHOOL OF MEDICINE, AND SCHOOL OF PHARMACY, UNIVERSITY OF WISCONSIN, MADISON
Communicated by Henry Lardy, December 3, 1965
Lincomycin is an antibiotic produced by Streptomyces lincolnensis and it has recently been shown to possess the following chemical structure.' CH3
CH3 /
N| \ ~~HO-CH
C \ CH2CH2CH3 / ~~C- NH\|
CH
0OH/H
S-CH3
