Recombinant interleukin 2 (a gift from Chiron Corporation, Emeryville,. Calif.) at a concentration of 30 IU/ml was added after 14 days of in vitro stimulation.
INFECTION AND IMMUNITY, Jan. 1996, p. 176–181 0019-9567/96/$04.0010 Copyright q 1996, American Society for Microbiology
Vol. 64, No. 1
Human CD41 and CD81 T Lymphocytes Are Both Cytotoxic to Toxoplasma gondii-Infected Cells JOSE G. MONTOYA,1,2,3 KAREN E. LOWE,4 CAROL CLAYBERGER,5 DEWEY MOODY,6 DUC DO,1 JACK S. REMINGTON,1,2 SOHEL TALIB,4 AND CARLOS S. SUBAUSTE1,2* Department of Immunology and Infectious Diseases, Research Institute, Palo Alto Medical Foundation, Palo Alto, California 943011; Division of Infectious Diseases, Department of Medicine,2 and Department of Cardiothoracic Surgery,5 Stanford University School of Medicine, Stanford, California 94305; Veterans Affairs Palo Alto Health Care System, Palo Alto, California 943043; and Department of Molecular Biology4 and Department of Infectious Diseases,6 Applied Immune Sciences, Inc., Santa Clara, California 95054 Received 6 June 1995/Returned for modification 3 July 1995/Accepted 27 October 1995
Studies to determine if Toxoplasma gondii-specific human T cells lyse parasite-infected cells have yielded conflicting results. Furthermore, attempts to obtain human cytotoxic CD81 T lymphocytes have been difficult because of the lack of a reproducible system for their generation. By using paraformaldehyde-fixed, T. gondii-infected peripheral blood mononuclear cells as antigen-presenting cells, we developed a method whereby T. gondii-specific T-cell lines can be reproducibly generated. Six T. gondii-specific T-cell lines were generated from an individual chronically infected with T. gondii. Cytofluorometric analysis of these lines revealed >99% CD31, 85 to 95% CD31 ab T-cell-receptor-positive (TCR1), 5 to 9% CD31 gd TCR1, 50 to 70% CD41, and 20 to 40% CD81 cells when cells were examined during the first 3 weeks of stimulation and >99% CD31, >99% CD31 ab TCR1,
