Human Genetics

4thHUGOPacificMeeting &

- PacificConferenc€on sthAsia

HumanGenetics

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October 27-30, 2OO2 AmbassadorCity Jomtien, Pattaya,Chonburi,Thailand rsBN 974-05-0173-7

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4thHUGOPacificMeeting &

5thAsia-PacificConferenceon HumanGenetics

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October27-30,2002 Ambassador CityJomtien, Pattaya,Chonburi, Thailand

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Natth Victor Vivian Zhu Richard Jean Nizam Yasufumi Changwon Ryszard Masami Michihiko Mark Edison Lucio Sangkot Victor Ivy Akihiko Sakol Sue Griffin Richard Yoshiyuki Alan Grant Lap-Chee Geft-Janvan Prawase Sir David Robert Huanming Zeng

Bhamarapravati Boulyjenkov Chan Chen Cotton Gonzalez Isa Kaneda Kang Kole Muramatsu Kuwano Lathrop Liu Luzzatto Mazuki McKusick r\l€ Okuyama Panyim Povey Rodgers Samulski Sakaki Schechter Sutherland Tsui Ommen Wasi Weatherall Williamson Yang Yi Tao

Thailand Switzerland Hong Kong,PRC PRC Australia France Malaysia Japan Korea USA Japan Japan France Singapore Italy Indonesia USA Singapore Japan Thailand UK USA USA Japan USA Australia Canada The Netherlands Thailand UK Australia PRC PRC

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Symposium11: Antisensetherapy

Laemchabang Room

Chairpersons: Yi Tao Zeng Duangrurdee Wattanasirichaigoon (RNA|) 1 6 : 0 0 - 1 6 : 3 0Intracellularly activeribozymes and siRNAs 5-035 KazunariTaira,Japan 1 6 : 3 0 - 1 7 : 0 0Develooment for B-thalassemia with 5-036 of treatments oligonucleotides and virallydelivered RNAs antisense RvszardKole,USA gene 1 7 : 0 01-7 : 3 0 Antisense oligonucleotides andthe dystrophin 5-037 : startingto makesomesense StephenWilton,Australia

SymposiumI2z Infection and diseasesusceptibilaty Mabtapud Room Chalrpersons: J.P. Gonzalez Sirirurg Songsivilai 1 6 : 0 0 - 1 6 : 3 0 Geneticanalysesof SSPEpatientsin Japanese Koichi Kusuhara, Japan 1 6 : 3 0 - 1 7 : 0 0Genomescreeningof clinicalmalaria Anavaj Sakuntabhai, France 17:00-17:30 Polymorphism and molecularmarkersin human genomeand susceptibility to diseasesan Indianinitiative Bamezai RameshwalIndia

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MG-05

USE ()F GEI\TETIC TOOI.S IN TEE rIDENTIFICATION OF'ARTHROPOI>BORNE BACTERIA THAT ARE PIOTENTIAL AGENTS OF. EMERGING ZOONOSES STUDY IN TEAILAND.

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Parola P, Wongsrichanalai C, MillerRSI and GonzalezIP Department of Immunology and Medicine, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok, Thailand, Unit? des Rickettsies, CNRS UMR 6020, IFR 48, Facult? de M?decine, Marseille, France, Institut de Recherche pour le D?veloppement UR34, Mahidol UniversitSr at Salaya, Nakhon Pathom, Thailand. In recent years, new molecular methods have enabled the development of useful, affordable and sensitive genetic tools to detect and identi$ pathogens in arthro@s which may act as vectors of emerging diseases in humanPrevious seroepidemiological data suggested possible risks of tick-and flea- borne diseases among rural residents living in the central part of the Thai-Myarrmar border (Sangkhlaburi District Iknchanaburi Province, Thailand). In an efFort to identiô/ potential etiologic arthropod-borne agents afFecting hurnans in this area , we analysed 65O ticks and 89 fleas collected from peridomestic and wild animals in Sangkhlaburi for evidence of bacterial infections. Specific primers ampli$ing genes of bacteria of the order Rickettsiales were used in PCR reactions followed by sequencing methods and phylogenetic studies. For the first time, a total of 13 bacteria of the genera Rickettsia, Anaplasma, Ehrlichia and Bartonella from the area were identified and phylogenetically characterized- These included known human pa.thogens, animal pathogens as well as basteria of unknown pathogenicity. Such findings are of epidemiological importance in an area where pa.tients presenting with fever of unknown origin are coûrrnon.

MG-06

OF THELEUBGENE MOLDCTIU\RCLONINGAND NUCLEOTIDESEQUENCE OF MEIOTHENAUSRUBER. Wan-Chee Tey*,Moo-EngLim#,andChong-Lek Koh*

*lnstitutesof BiologicalSciences (Genetics) andrPostgraduate Studies, Universityof Malaya,50603KualaLumpur,Malaysia. Tlte IeuB geneof Meiothermusruber, a moderatethermophile,wffi clonedby genetic of the leuB mttaÎjronin Escherichiacoli HBl0 I . It encodesthe enzymepcomplementation (p-IPMDH;EC 1.1.1.85) isopropylmalate dehydrogenase that catalyzes theconversion of Bp-ketoisocaproate in the leucine biosynthesispathway. It wiu isopropylmalateto 1,053bp encoding350 aminoresidues. and found to comprise The subsequently sequenced lower than the GC contentsof extreme GC contentof M. ruber IeuB genewas 67.620/o, thermophilicbacteriabut higherthantheGC contentsof mesophilicbacteria.Themolecular weightof the deducedprot€inproductof M. ruber leuB genewir calculated to be 38,889. The deducedaminoacid sequence of M. ruber P-IPMDHshowedthe highestsimilariw (77%) with B-lPMDH of Ther mutther mophiIusl{B,8.