Comparative Cytotoxicity of Various Drug Combinations for Human Leukemic Cells and Normal Hematopoietic Precursors Tai-Tsung Chang, Subhash Gulati, Ting-Chao Chou, et al. Cancer Res 1987;47:119-122. Published online January 1, 1987.
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[CANCER RESEARCH 47, 119-122, January l, 1987)
Comparative Cytotoxicity of Various Drug Combinations for Human Leukemic Cells and Normal Hematopoietic Precursors1 Tai-Tsung Chang,2 Subhash Gulati,3 Ting-Chao Chou, Michael Colvin, and Bayard Clarkson Hematology / Lymphoma Service, Department of Medicine ¡T.T. C., S. C. G., B. C.J and Pharmacology- Laboratory ¡T-C.C.J, Memorial Sloan- Kettering Cancer Center, New York, Netv York 10021, and the Department of Pharmacology, Johns Hopkins University Oncology Center, Baltimore, Maryland 21205 [M. C.J
ABSTRACT The development of suitable methods for purging the malignant cells contaminating the bone marrow of patients with cancer may offer a better chance of success for autologous bone marrow transplantation. In this paper, we further describe our efforts at purging acute myelogenous leukemia cells. 111.-6(1,a promyelocytic leukemia cell line, was used as a model. 4-Hydroperoxycyclophosphamide (4-HC), VP-16-213 (VP-16), and Adriamycin were used alone or in combination to develop the best method to purge HL-60 cells. The cytotoxicity of 29.2 »ig/ml(100 UM) of 4-HC was 99.8 ±0.12% (SD) on HL-60 cells and 82.5% on colony forming units-granulocyte, macrophage. Ninety-nine % of HL-60 cells and 72.7% of colony forming units-granulocyte, macrophage were inhib ited by VP-16 at a concentration of 25 ¿ig/ml(42.5
