Human Leukemic Cells and Normal Hematopoietic ...

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Comparative Cytotoxicity of Various Drug Combinations for Human Leukemic. Cells and .... of media (10% PCS in RPMI) from which the cell count and viability ..... Sharkis, S. J., Sanios, G. W., and Colvin, M. !•Mulinatimi of acute myeloge.
Comparative Cytotoxicity of Various Drug Combinations for Human Leukemic Cells and Normal Hematopoietic Precursors Tai-Tsung Chang, Subhash Gulati, Ting-Chao Chou, et al. Cancer Res 1987;47:119-122. Published online January 1, 1987.

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[CANCER RESEARCH 47, 119-122, January l, 1987)

Comparative Cytotoxicity of Various Drug Combinations for Human Leukemic Cells and Normal Hematopoietic Precursors1 Tai-Tsung Chang,2 Subhash Gulati,3 Ting-Chao Chou, Michael Colvin, and Bayard Clarkson Hematology / Lymphoma Service, Department of Medicine ¡T.T. C., S. C. G., B. C.J and Pharmacology- Laboratory ¡T-C.C.J, Memorial Sloan- Kettering Cancer Center, New York, Netv York 10021, and the Department of Pharmacology, Johns Hopkins University Oncology Center, Baltimore, Maryland 21205 [M. C.J

ABSTRACT The development of suitable methods for purging the malignant cells contaminating the bone marrow of patients with cancer may offer a better chance of success for autologous bone marrow transplantation. In this paper, we further describe our efforts at purging acute myelogenous leukemia cells. 111.-6(1,a promyelocytic leukemia cell line, was used as a model. 4-Hydroperoxycyclophosphamide (4-HC), VP-16-213 (VP-16), and Adriamycin were used alone or in combination to develop the best method to purge HL-60 cells. The cytotoxicity of 29.2 »ig/ml(100 UM) of 4-HC was 99.8 ±0.12% (SD) on HL-60 cells and 82.5% on colony forming units-granulocyte, macrophage. Ninety-nine % of HL-60 cells and 72.7% of colony forming units-granulocyte, macrophage were inhib ited by VP-16 at a concentration of 25 ¿ig/ml(42.5