hydrocortisone in patients with chronic lymphocytic ...

From bloodjournal.hematologylibrary.org at CAPES CONSORTIUM on July 9, 2011. For personal use only.

1977 49: 719-728

Variations in lymphocyte counts four hours after administration of hydrocortisone in patients with chronic lymphocytic leukemia G Dighiero, G Vaugier, D Charron, P d'Athis and JL Binet

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From bloodjournal.hematologylibrary.org at CAPES CONSORTIUM on July 9, 2011. For personal use only.

Variations After in Patients

in Lymphocyte Administration With Chronic

By Guillermo

Dighiero, Philippe

Counts Four Hours of Hydrocortisone Lymphocytic Leukemia

Georges d’Athis,

Vaugier,

and

HE EFFECT

ofcorticosteroids

gators days tion

following whatever

shown

4-6

that

the

hr after

i.v.

been

our

of

administration,

aim

to compare

CLL,

the

and

test.

differential

white

Apparatus. D,3’4

cytochemical

obtained

and

response

From

the

Address ©

Blood,

automatic

obtained

and

differential

and

a cell

Department July for

of Hematology,

2, 1976; reprint

accepted requests:

Professor

Vol.

49,

No.

& Stratton,

5 (May),

1977

injection prior

have a peak

These

studied

were

of 400

4 hr after performed

were classifies

Lymphocytes

used (peroxidase,

Hopitalier

mg

to and

that

J.L.

Dale2

results,

protocols:

in normal

counts

10.

OHC).

different

the

variable.

subjects

as those used anatomical-clinical

white

Groupe

mg

time

analyzer histogram.

January

Piti#{233}-Salp#{234}tri#{232}re, 91 Boulevard

1977 by Grune

size

reactions

and reaches

by

It

and Fauci data

in and and

METHODS iv.

immediately

multichannel

observed no variaafter i.v. injection

Fauci

using

observed

AND a single

count in paMost investiduring the few

lymphocytes

doses of OHC with age, sex,

received

to the three cytochemical

Submitted pitalier

were

counts

an properties

response

subjects

count

Blood

Hemalog

All

subjects, (400

were

MATERIALS

Hydrocortisone

et al.’ have a few hours

peripheral

response

using

the same and to correlate the results membrane markers.

Dale,2 surface

Shaw count

of hydrocortisone authors,

lymphocyte is variable. count

(CLL) lymphocyte

In normal

number

administration of

with

patients.

in the

by various

method

patients

in four

drop

substantiated doses,

leukemia peripheral

i.v. injection of prednisone. in the peripheral lymphocyte

of 100 mg prednisone

has

on the peripheral

tients with chronic lymphocytic have reported a rise in the

Charron,

Binet

phocytic leukemia (CLL), the peripheral lymphocyte count showed a variable response: decrease, no change, or increase. A correlation was shown to exist between a decrease in peripheral lymphocyte counts and anatomical-clinical staging: patients with involvement restricted to blood and bone marrow very often exhibited a drop in their peripheral lymphocyte count ( p 4000/cu the

Ages high

that

patients

The

other 1.18

as compared

As

the

study

to

of the

129

patients

and

did

again

l).

cases

of CLL

by

to

three (2)

this

study from

of

10 g/lOO




injection

were

that

groups

in the III;

basis

to influence the conclusions. this anatomical-clinical staging

distinct up

stage

time were

this

differ

the

infiltration

There

that

demonstrate

do not appear Rai et al.,6

followed II;

in from

in not

on

compared

to Videbaek, was

Indeed,

I, and

as

differ

it did

work

IV.

23 patients

interest

not

made

at the

4 stage

0 patients

1.4 according

of this

(Fig.

(I)stagesO,

lymphocyte

distribution

once

purpose

was

a particular

others, these differences in the publication

prognosis pital:

took

present

of CLL marrow

CLL III,

of stage

us

but

43

with

mm).

39 to 88 yr, and

male-female

authors,

Since and

of

bone

10 stage from

percentage

in the

tients.7

II,

ranged

one

diagnosis with

of the

1, 3 stage

patients.

initial

lymphocytosis

100,000/cu