Hypermethylation of brain natriuretic peptide (BNP)

BIOSCIENCE REPORTS

ACCEPTED MANUSCRIPT Hypermethylation of brain natriuretic peptide (BNP) gene is associated  with the risk of rheumatic heart disease    Ni Li, Dawei Zheng, Lebo Sun, Huoshun Shi, Xiuying Zhu, Guodong Xu , Qinning Wang,  Caimin Zhu, Guofeng Shao  To investigate the contribution of BNP promoter DNA methylation to the risk of  rheumatic heart disease (RHD) and the influence of warfarin anticoagulant therapy on  BNP methylation levels for RHD patients after surgery.BNP methylation levels were  determined by bisulfite pyrosequencing from plasma samples of RHD patients compared  with healthy controls. Several factors influencing the RHD patients were included age,  smoking, and cholesterol levels. A fragment of 5 CG sites (CpG1‐5) in the promoter region  of BNP gene was measured. BNP gene hypermethylation was found in CpG4 and CpG5 in  RHD patients compared with non‐RHD controls. A significant difference was also  observed between RHD patients with long‐term administration of warfarin and RHD  patients who had recently undergone an operation. Moreover, single CpG4 and CpG5  analysis revealed a significant increase in methylation levels in men. BNP gene‐body  hypermethylation is associated with the risk of RHD, and also influenced by the warfarin  anticoagulant therapy of RHD patients after surgery, which could represent novel and  promising targets for therapeutic development. 

Cite as Bioscience Reports (2016) DOI: 10.1042/BSR20160408

Copyright 2016 The Author(s). This is an Accepted Manuscript; not the final Version of Record. You are encouraged to use the final Version of Record that, when published, will replace this manuscript and be freely available under a Creative Commons licence. All other rights reserved.

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