and Gynecology, CHA University, College of Medicine, Seoul, Korea, Repub- ..... K. A. Burns.b aObstetrics and Gynecology, University of. Cincinnati, West ...
in hypercholesterolemia in offspring until adulthood. The lncRNA microarray covering a vast majority of the expressed RefSeq transcripts showed 316 upregulated and 317 downregulated lncRNAs in fetal mouse livers exposure to high E2. We also identified a downregulated lncRNA-Gm5196 in HepG2 cells treated with E2. In addition, knockdown of lncRNA-Gm5196 increased cholesterol synthesis and the expression of osbpl5, a key factor of intracellular cholesterol transport. CONCLUSIONS: High intrauterine E2 exposure could result in dyslipidemia in offspring, which could not be completely recovered in adulthood. The lncRNA expression profiles were significantly altered in fetal mouse livers exposure to high E2 environment and E2 regulated the expression of lncRNAGm5196 in HepG2 cells, suggesting a cholesterol-suppressive role of lncRNAGm5196 by affecting Osbpl5. Thus, the maternal E2 level must be monitored precisely, and lncRNA-Gm5196 could be a potential target to prevent the lipid disorders in offspring induced by maternal high E2 environment. Supported by: This work was supported by the National Natural Science Foundation of China (No.81671519) and the Central Guided Local Development of Science and Technology Special Fund (No.2016080802D114). P-574 Wednesday, November 1, 2017 AMBIENT AIR POLLUTION IS ASSOCIATED WITH SUCCESS OF IN-VITRO FERTILIZATION CYCLES: A RETROSPECTIVE STUDY IN SEOUL, KOREA. S. Choe,a S. Kim,b Y. Jun,c W. Lee,d I. Kang,e M. Koong,f J. Heo,g C. Sim,h Y. Koh,i D. Park,a T. K. Yoon.j aObstetrics and Gynecology, CHA University, College of Medicine, Seoul, Korea, Republic of; bInstitute of Health & Environment, Seoul National University, Seoul, Korea, Republic of; cSeoul National University, Seoul, Korea, Republic of; d Ob/Gyn Dept., Gangnam CHA Hospital, Seoul, Korea, Republic of; eObstetrics and Gynecology, CHA University School of Medicine, Seoul, Korea, Republic of; fCHA University School of Medicine, Seoul, Korea, Republic of; g Fertility Center, CHA Gangnam Medical Center, Seoul, Korea, Republic of; h Gangnam CHA Infertility Center, Gangnam CHA Hospital, Seoul, Korea, Republic of; iFertility Center, CHA Gangnam Medical Center, CHA University, Seoul, Korea, Republic of; jFertility Center, CHA Gangnam Medical Center, College of Medicine, CHA University, Seoul, Korea, Republic of. OBJECTIVE: To explore the relationship between the average level of air pollutants and probabilities of intrauterine pregnancy per cycle in women who underwent one or more in-vitro fertilization (IVF) cycles. DESIGN: A retrospective cohort study. MATERIALS AND METHODS: We analyzed 6,621 cycles of 4,581 patients who had underwent one or more fresh IVF cycles at a single IVF center from January 2006 to December 2014 and lived in Seoul at the time of IVF treatment. Daily concentrations of five major air pollutions including fine particulate matter (PM10), nitrogen dioxide (NO2), carbon monoxide (CO), sulfur dioxide (SO2), and ozone (O3) measured at 40 regulatory monitoring sites in Seoul were averaged for each of the four periods for estimating individual exposure. These periods were: period 1 (from start of controlled ovarian stimulation (COS) to oocyte retrieval), period 2 (from oocyte retrieval (OR) to embryo transfer (ET)), period 3 (from ET to serum pregnancy test), and period 4 (from start of COS to serum pregnancy test). Generalized linear mixed models (GLMM) were used for estimating probability of pregnancy according to interquartile range (IQR) increase of five air pollutants during each period accounting for correlation of treatments within each individual. Covariates were women’s age, body mass index, number of retrieved oocytes, intracytoplasmic injection, blastocyst transfer, season, and the year. RESULTS: Median age of the study population was 35 years. Mean body mass index was 20.9 kg/m2 and mean number of repeated IVF cycles was 1.4. Cumulative pregnancy rate was 51.3% (2,348/4,581). In the GLMM analysis, IQR increases in NO2 and CO during the period 1 were related with decreased probability of intrauterine pregnancy (adjusted odd ratio¼0.89 [95% confidence interval¼0.82, 0.97] and 0.92 [0.84, 1.00]). In addition, we found the inverse association for PM10, NO2, and CO during the period 3 and achievement of intrauterine pregnancy in each cycle (0.87 [0.79, 0.95], 0.84 [0.76, 0.92], and 0.92 [0.85, 1.00], respectively). CONCLUSIONS: Increased concentrations of ambient PM10, NO2, and CO during COS and after embryo transfer were related with decreased probability of intrauterine pregnancy in IVF cycles. Supported by: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2013R1A6A3A04059017). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
FERTILITY & STERILITYÒ
P-575 Wednesday, November 1, 2017 DECLINE IN SEMEN QUALITY OF BRAZILIAN MEN INVESTIGATED FOR INFERTILITY IN THE LAST 27 YEARS. L. F. Baccaro, A. C. Ropelle, J. A. Nascimento, F. A. Fazano, M. Sousa, J. R. Erbolato Gabiatti, L. CostaPaiva. Obstetrics and Gynecology, University of Campinas (UNICAMP), Campinas, Brazil. OBJECTIVE: In the last decades, there have been questions about a possible decline in seminal quality of men around the world and its possible consequences for human species. Our objective was to evaluate seminal parameters of Brazilian men investigated for infertility in the last 27 years. DESIGN: Retrospective analysis of spermograms performed at University of Campinas - Brazil. MATERIALS AND METHODS: The study population was composed of all male partners of couples admitted for infertility between 01/1989 and 12/ 2016. Samples of men known to have some deleterious condition to seminal quality, samples used for in vitro fertilization or intrauterine insemination, and samples produced by voluntary semen donors were excluded. The dependent variables were sperm concentration before (SCB) and after semen processing (SCA), percentage of spermatozoa with progressive motility (PM) and percentage of spermatozoa with normal morphology (NM). The independent variable was the sample’s year of collection categorized into five groups: % 1995; 1996-2000; 2001-2005; 2006-2010; > 2010. The age of man at sample collection and other parameters of the spermogram were considered as control variables. Statistical analysis was carried out using Kruskal-Wallis test and generalized linear models. RESULTS: 18902 samples produced by 9495 men were analyzed. The mean number of samples per subject was 1.99 (0.73). Throughout the study period, the mean age of men was 32.92 (6.54) and the median was 32.30 years (range 18.10-67.42). For 8562 samples, there was also data on seminal processing. The means of the four dependent variables declined during the analyzed period. SCB decreased from 86.42 million/ml in the period % 1995 to 48.32 million/ml in the period > 2010 (p 2010 (p 2010 (p 2010 (p
