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Articles in PresS. Am J Physiol Regul Integr Comp Physiol (November 11, 2015). doi:10.1152/ajpregu.00395.2015

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Hyperthermia, dehydration and osmotic stress: unconventional sources of exercise-induced reactive oxygen species

5 6 7 Michelle A. King1, Thomas L. Clanton1, Orlando Laitano1,2

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University of Florida, Applied Physiology and Kinesiology, USA

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Universidade Federal do Vale do São Francisco, Colegiado de Educação Física, Brazil

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Corresponding Author: Orlando Laitano University of Florida Department of Applied Physiology and Kinesiology College of Health and Human Performance 100FLG Box 118205 Gainesville, FL, 32611 Fax: 352-392-0316 Phone: 352-392-0584 e-mail: [email protected]

Copyright © 2015 by the American Physiological Society.

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Abstract

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Evidence of increased reactive oxygen species (ROS) production is observed in the circulation

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during exercise in humans. This is exacerbated at elevated body temperatures and attenuated

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when normal exercise-induced body temperature elevations are suppressed. Why ROS

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production during exercise is temperature dependent is entirely unknown. This review covers

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the human exercise studies to date that provide evidence that oxidant and antioxidant changes

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observed in the blood during exercise are dependent upon temperature and fluid balance. We

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then address possible mechanisms linking exercise with these variables that include shear

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stress, effects of hemoconcentration, and signaling pathways involving muscle osmoregulation.

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Since pathways of muscle osmoregulation are rarely discussed in this context, we provide a

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brief review of what is currently known and unknown about muscle osmoregulation and how it

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may be linked to oxidant production in exercise and hyperthermia. Both the circulation and the

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exercising muscle fibers become concentrated with osmolytes during exercise in the heat,

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resulting in a competition for available water across the muscle sarcolemma and other tissues.

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We conclude that though multiple mechanisms may be responsible for the changes in

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oxidant/antioxidant balance in the blood during exercise, a strong case can be made that a

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significant component of ROS produced during some forms of exercise reflect requirements of

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adapting to osmotic challenges, hyperthermia challenges and loss of circulating fluid volume.

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Key-words: heat stress, skeletal muscle, oxidative stress, lipoxygenase, taurine

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Introduction Reactive oxygen species (ROS) produced during exercise has been a topic of much

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interest since its introduction over 30 years ago (16, 18). It is well established that skeletal

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muscle serves as a source of ROS during contraction (38, 70, 71) but the functional significance

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of exercise-induced ROS remains unclear and paradoxical. For example, while large amounts

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of ROS produced during intense contraction can potentially damage cells (70), low to moderate

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levels of ROS can promote the expression of an oxidative phenotype in muscle (65, 73) and can

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have important regulatory effects on muscle blood flow (19, 26). Also, a number of

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environmental and metabolic factors associated with exercise can induce ROS, such as

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hyperthermia, dehydration, and osmotic stress (34, 35, 42, 43, 51, 59, 67, 93). However, the

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relative importance of these additional environmental factors in exercise is poorly defined. The

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aim of this review is to broaden the framework of discussion regarding the sources of ROS

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production during exercise, specifically focusing on the potential influences of hyperthermia,

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dehydration and osmotic stress on this phenomenon.

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Hyperthermia-induced reactive oxygen: insights from cells, animal models, and human

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experiments

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As an individual exercises, core temperatures increase as a function of exercise intensity

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and duration, occurring to a greater extent in warm and humid environments. Exercising muscle

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temperatures can run 0.7-1°C above core temperature during exercise at ~75% of VO2max with

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duration ranging from 30 to 90 min (63); therefore, during prolonged (~60 min or above)

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moderate to high intensity (>70% VO2max) exercise in warm environments, muscles can

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experience temperatures surpassing 41°C. Likewise, passive heating increases ROS

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production in skeletal muscle. For example, rat diaphragm generates elevated intracellular and

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extracellular superoxide (O2−) within a few minutes of exposure to 42°C (93). Although ROS

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can affect muscle force production, skeletal muscle, fortunately, has a relatively high heat

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tolerance. This tolerance is specific to muscle type, as intact rat diaphragm is resistant to

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temperature elevations up to 41°C (59); although mouse soleus has a ≈20% decay in force after

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1 hour exposure at this temperature (85). Smaller elevations in temperature may affect long-

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term muscle function. For example, exposure of mouse extensor digitorum longus fibers to

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40°C results in a leaky sarcoplasmic reticulum (SR) Ca+2 pump (82). This effect can be blocked

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by O2− scavengers and reversed by reducing agents that act on oxidized sulfhydryls (82).

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Mechanisms for heat-induced ROS formation in muscle remain elusive. Both

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mitochondrial and non-mitochondrial sources have been proposed. One popular hypothesis is

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based on the observation that muscle mitochondria uncouple in a temperature-dependent

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manner during hyperthermia of >40°C (8). Uncoupling was historically thought to lead to

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production of mitochondrial O2- (75). However this concept has been challenged on both

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theoretical and experimental grounds (66). For example, recent work demonstrates that

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hyperthermia elevates mitochondrial membrane potential, a state that directly leads to

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mitochondrial ROS formation (40). This has been shown to be chiefly responsible for elevations

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in ROS formed from isolated muscle mitochondria when exposed to hyperthermia (41). Whether

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mitochondria are responsible for ROS, in vivo, is still unknown, and attempts to apply

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conventional approaches to block mitochondrial ROS have been unsuccessful (94). ROS

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formation generated in muscle during exercise may not be related to the responses seen in

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hyperthermia. Growing evidence reveals that mitochondria produce more ROS during state 4

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(basal) respiration (typical of a resting state) compared with state 3 (maximal ADP-stimulated

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respiration) (4, 17, 54). This is important since during aerobic contractile activity skeletal muscle

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mitochondria are in state 3, thus limiting generation of ROS during contractions (33). What this

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means during exercise in hyperthermia is unknown, but it is possible that exercise-induced

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shifts to state 3 respiration suppress the mitochondrial ROS formation associated with

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hyperthermia in resting muscle, due to the shift of mitochondria to state 3-ADP-dependent

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respiration.

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Other ROS generating pathways may be equally important. For example, heat exposure

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in vitro activates phospholipases (PLA), causing release of arachidonic acid and other

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metabolites (12). Some forms of PLA exist in the cytosol, on plasma, nuclear membranes, and

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embedded in mitochondrial membranes (69). In general, PLA activity supports ROS formation in

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muscle (27, 55), and pharmacological inhibition of all phospholipases suppresses skeletal

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muscle extracellular ROS formation during heat exposure (92). PLAs produce arachidonic acid,

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which serves as a substrate for generation of prostaglandins, leukotrienes, and the other

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eicosanoids that have many signaling functions. Blocking lipoxygenase during heat almost

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completely blocks extracellular O2− formation in muscle, whereas blocking cyclooxygenases

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increases O2− formation, suggesting a critical role of specific eicosanoid metabolic pathways in

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management of extracellular oxidants in heat (92). Furthermore, these pathways are linked to

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function. By blocking ROS signaling using pharmacological inhibition of eicosanoid forming

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enzymes, Oliver et al. (59) showed that all of the eicosanoid metabolic pathways studied appear

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to be functionally important in the contractile response to hyperthermia, i.e. pharmacological

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blockade of phospholipases, lipoxygenases or cyclooxygenases, resulted in significant

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reductions in tetanic force and often induced background muscle contracture in heat. In the

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absence of heat, there were no effects of these pharmacological inhibitors. This may, in part,

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reflect disturbances in the cell volume regulatory mechanisms of muscle, which are dependent

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on these pathways, as discussed later in the review.

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Skeletal muscle is not the only source of ROS during hyperthermia. For instance, the

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immune system also plays a pivotal role in oxidative balance following heat exposure. The

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maximal core temperature reached during exercise is a key factor of protein oxidative damage

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in lymphocytes. In fact, the ratio of oxidants to antioxidants in lymphocytes and neutrophils is

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affected by high intensity exercise (79). When subjects ran for 45 min at 75-80% VO2max in the

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heat (32˚C, ~75% relative humidity), reaching maximal core temperatures of 39.8°C,

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lymphocyte protein oxidative damage was increased. Conversely, when exercise was

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performed in a cold, temperate environment (10˚C, 45% RH) subjects’ core temperature only

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reached 38°C and this effect was not observed (52). Additionally, antioxidant systems were

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upregulated in the heat at high core temperatures as evidenced by increases in catalase and

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glutathione reductase enzyme activities (52). Other evidence that the immune system can be an

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important source of circulatory oxidants during heat stress comes from an in vitro study showing

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that human leukocytes display an increase in oxidative damage after incubation at 40°C,

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following a 2h-treadmill exercise session, where participants jogged at 4-6 km/h for 30-45 min

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and walked up a 2-10% grade for 75-90 min (74).

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Changes in splanchnic blood flow that occur during hyperthermia might be another

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important source of ROS. After exposure to several days of low-level heat stress, rat intestine

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shows strong evidence of extensive oxidative damage, and isolated intestinal segments

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demonstrate oxidation of proteins and elevations in permeability at 41°C that can be blocked

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with antioxidant treatments (58). This is no doubt a more chronic response. In more acute

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settings, heat-induced increases in intestinal permeability are in part a consequence of

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increased ischemia/reperfusion due to local changes in splanchnic blood flow (58). For instance,

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Hall et al. (30) demonstrated that when core temperature in rats was passively elevated to

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41.5°C, splanchnic blood flow was decreased by 40%, leading to the production of

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ceruloplasmin, semiquinone, penta-coordinate iron (II), and nitrosyl heme. This suggests that

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reduced splanchnic blood flow is likely a mechanism for increased in ROS generation, which

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then may exacerbate intestinal and circulatory dysfunction (30). These responses may be

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amplified during exercise in the heat, as fluid loss in the form of sweat further reduces blood

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volume and splanchnic blood flow (25). In support of this hypothesis, Hall et al. demonstrated

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that ROS formation was locally stimulated in the splanchnic circulation following passive whole

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body heating in rats (29). Altogether, these data indicate that hyperthermia affects a variety of

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tissues besides skeletal muscle, which could potentially influence the redox status of the

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plasma. Additional factors, indirectly related to either exercise or hyperthermia, may also

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contribute. For example, vascular fluid loss in the form of sweat or muscle swelling can lead to

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dehydration. As will be discussed later in the review, dehydration may enhance ROS

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production because of its influence on blood viscosity, vascular shear stress and osmotic

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gradients (21, 28).

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In tissues from more chronic animal studies, oxidant production also leads to an

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increased activity of antioxidants in order to protect redox homeostasis and build a defense

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against future insults. Some studies have shown increases in antioxidant enzymes such as

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superoxide dismutase (SOD) with heat stress alone (31, 47, 53). Similarly, it has been

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demonstrated that when antioxidants are chronically lowered, particularly SOD, the ability to

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withstand hyperthermia decreases (60). Collectively, these data support the notion that

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adaptations to hyperthermia and antioxidant defense mechanisms are upregulated to protect

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cells or tissues from excessive ROS formation induced by heat or other associated stress-

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signaling events.

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Hyperthermia and ROS formation in resting and exercising humans Human experiments suggest that many of the observations in isolated muscles or other

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tissues may directly translate to humans, as summarized in Table 1. In one of the first human

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experiments with passive heat exposure studying biomarkers of ROS-induced damage,

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Ohtsuka et al. (57) passively submerged subjects in 42°C water for 10 min. Significant

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decreases in the antioxidant substrate, glutathione (GSH), were observed in red blood cells.

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Circulating lipid peroxides (a measure of ongoing oxidative damage) were also elevated. In

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contrast, immersion in 25°C water increased GSH in red blood cells, with no change in markers

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of lipid peroxidation in the blood. The authors concluded that oxidative stress is produced by

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short-term (10 min) passive exposure to a warm environment, while exposure to a cooler

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environment stimulates blood antioxidant defenses (57).

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McAnulty et al. (51) employed treadmill running for ~50 min at 50% VO2max in humans

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in a hyperthermic environment (35°C, 75% relative humidity (RH)), compared to a normothermic

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environment (25°C, 40% RH). F2-isoprostanes (an arachidonic acid-derived indicator of lipid

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peroxidation) were significantly elevated in hyperthermia, above those measured in

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normothermia, providing evidence that increases in core temperature boost ROS production in

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tissue compartments having access to the circulation. Of note, participants in the hyperthermic

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group were dehydrated by ~3% of initial body weight. Similar findings were reported by Pilch et

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al. (64) who determined changes in blood oxidant/anti-oxidant balance when core temperature

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was elevated by 1.2°C via a passive heat source (sauna) or when core temperature was

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elevated to the same extent by cycling in a hot environment (33°C/70% RH). Both hyperthermia

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exposures caused reductions in the antioxidant capacity of blood and elevations in lipid

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peroxidation, though the reduction in antioxidant capacity was more evident in passive heat

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exposure than in exercise-induced heat in untrained subjects. Resembling the study of

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McAnulty et al. (51) the subjects exhibited large reductions in plasma volume during the

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experiment, with average values ranging from 7-10% between groups (64). Therefore, in both of

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these studies it is difficult to attribute the elevations in ROS to increased core temperatures

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alone because of vascular dehydration.

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Sureda et al. (80) and Mestre-Alfaro et al. (52) demonstrated the importance of core

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temperature in augmenting the antioxidant and anti-inflammatory status. In both studies

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subjects exercised for 45 min at 75-80% of VO2 max and were exposed to either a hot

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environmental condition of 30-32°C (75-78% relative humidity) or a cooler condition of 10-12°C

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(40-55% relative humidity), where maximal core temperatures were ≈39.4-39.8°C. It was found

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that both circulatory markers of oxidative damage and activity of antioxidant enzymes were

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significantly increased following exercise in the heat (80).

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To address the possibility that dehydration could be a confounder to the effects of

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exercise or exercise in hyperthermia, Laitano et al. (43) tested the effects of hyperthermic

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exercise while compensating for net dehydration. Participants were subjected to hyperthermia

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via a water-perfused suit and performed one–legged knee extensor exercise at 50% of pre-

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established peak power output, compared to resting conditions. Blood samples were taken

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before exercise and at the fifth minute of one-legged knee extensor exercise. Subjects

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recovered for 15 min and then were passively subjected to an increase in core temperature by

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1.2°C and a skin temperature of +6°C. Once the target temperature was reached, a blood

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sample was taken, followed by a repeated knee extensor exercise in the heated condition. A

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subsequent blood sample was taken after the fifth minute of heated single-legged exercise.

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Importantly, subjects were kept euhydrated by ingesting 1.5 L of warm water throughout the

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heat stress protocol, matching net water loss. At rest, ~75 min of passive heat increased blood

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oxidized glutathione (GSSG), but there was no change in GSH, which led to a decrease in the

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GSH/GSSG ratio, an indicator of oxidative stress. However, when heat stress was combined

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with one legged-knee extensor exercise, GSH and GSSG were both increased, resulting in no

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change in the GSH/GSSG ratio. This suggests passive heat exposure results in increased

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oxidant production at rest, but when combined with moderate intensity resistance exercise there

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are compensatory responses that may keep blood redox state in relative equilibrium. The

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preservation in GSH/GSSG ratio in the face of rising GSSG reflects exercise-induced GSH

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release into the circulation, confirmed via direct measurements of net release of GSH across the

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exercising limb vascular bed (42). Though rarely discussed, GSH can be actively secreted from

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skeletal muscle fibers in response to stress stimuli present in exercise or in heat (15, 42) and

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this could theoretically provide antioxidant support to the blood and to other organ systems

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during exercise in stressful environments.

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Quindry et al. (67) also assessed the effects of environmental temperature on markers of

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oxidative stress during exercise while subjects remained euhydrated as measured by body

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weight. Subjects performed cycle ergometry at a moderate intensity (60% of maximal power

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output) for 60 min while exposed to 7, 20, and 33°C. Recovery occurred in the same

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environmental temperature as their respective trials. Subjects were hydrated, during both the

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exercise and recovery phases, by ingesting approximately 1.2 L of H2O. Core temperatures

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were elevated with increased environmental temperatures in both the exercise and recovery

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phases. The authors measured a panel of oxidative stress biomarkers, which accounted for

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aqueous and lipid phase antioxidants and several measures of oxidative stress. Results were

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corrected for changes in plasma volume. Exercise and recovery in a warm environment led to

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greater oxidative stress responses compared to exercise and recovery in cooler environments.

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Interestingly, exercise in heated environments resulted in a greater antioxidant capacity in the

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blood.

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The studies reviewed in this section included a variety of heat stress and exercise

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protocols as highlighted in Table 1. For instance, some studies employed the whole-body

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passive heat stress through a water-perfused suit (43), sauna (64) or warm water immersion

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(57) whereas others achieved heat stress by exercising in a warm environment (51, 52, 64, 67,

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80). Likewise a blend of exercise types, duration, and intensities were used such as the short-

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term one-legged knee extensor exercise (43), mid to long-term cycling (64, 67) or running (51).

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Although it is unlikely that all changes herein reported are taking place under all conditions, it is

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possible to summarize that hyperthermia in man, with or without exercise, irrespective of

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hydration status causes the plasma to shift to a more oxidized state (Fig. 1). The response to

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exercise hyperthermia appears to be more influenced by hyperthermia than by exercise, and

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there is accumulating evidence that exercise may superimpose an increase in antioxidant

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capacity of the blood, potentially serving a protective function.

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Effects of dehydration and hemoconcentration on ROS formation during exercise in man

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There has been considerable interest in the effects of dehydration (acute reductions

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greater than 2% of body mass) on exercise performance, and it has been fairly well established

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(not without debate) that higher levels of sweat-induced dehydration can limit exercise

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performance (76, 86). Many mechanisms for the effects of dehydration on performance have

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been proposed, but one potential mechanism is through the effects of dehydration on redox

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status during exercise as suggested by human experiments summarized in Table 2 (35, 42, 62).

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Hillman et al. (35) investigated the effects of exercise-induced dehydration, with and

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without hyperthermia, on oxidative stress. Trained male cyclists completed 90 min of intense

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cycling exercise at 95% lactate threshold (LT). This was followed by a 5-km time trial in a warm

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or a thermoneutral environment. They found that oxidized glutathione (GSSG) increased

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significantly post exercise during a ~3.8% dehydration, while euhydration attenuated these

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increases, regardless of the environmental temperature. In a follow-up experiment, the same

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research group compared the effects of hyperhydration on oxidative stress, thermoregulation,

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and cycle performance (34). Trained males consumed glycerol or water to achieve

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hyperhydration, followed by a 90 min time trial. Total GSH increased post exercise in all trials,

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GSSG and protein carbonyl concentrations were increased post exercise for the control trial

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only. The study concluded that fluid intake attenuated oxidative stress associated with exercise,

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but did not enhance thermoregulation or performance.

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Other investigators have examined the influence of rehydration on oxidative stress following a period of dehydration (42, 62). One particular study by Paik et al. (62) induced 3%

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dehydration by passive heating in a sauna, followed by an exhaustive bout of treadmill running.

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Although these authors did not monitor core temperature they found dehydration increased

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oxidative lymphocyte DNA damage during treadmill running and this damage was reversed by

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fluid replacement with either water or a sports drink. In another investigation, Laitano et al.

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tested one-legged knee extensor exercise in both a dehydrated and rehydrated state in humans

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(42). Participants underwent graded dehydration by 0, 2, or 3.5 % of body mass, were then

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exercised and subsequently rehydrated. Dehydration in exercise resulted in significant

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elevations in a net release of GSH into the blood from the exercising muscle, but without

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significant elevations in GSSG or indicators of oxidative stress. Venous SOD activity was

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significantly decreased in the dehydrated state compared to the euhydrated state. These

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experiments support the concept that vascular dehydration may contribute to the oxidative state

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of the blood during exercise, particularly when core temperature is elevated.

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Potential mechanisms for increased ROS formation during dehydration

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The total peripheral resistance to blood flow within the vasculature is regulated in part by

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the caliber of the vessels and the viscous characteristics of the blood (21). During exercise, fluid

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shifts from the intravascular to both the interstitial and intracellular space. Sweat-induced

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dehydration results in fluid shifts and increases both blood osmolality and viscosity. This results

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in increases in shear stress as the blood interacts with the vascular wall. Hyperthermia and

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exercise can induce a state of hemoconcentration (9) due to the effects of sweat loss, filtration

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through capillary leakage and uptake of water in exercising muscles (83). Hemoconcentration,

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increases in viscosity, and changes in shear stress have been shown to occur in both maximal

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and submaximal exercise (45, 88). Hemoconcentration has been demonstrated repeatedly in

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exercise, even in the absence of sweat loss (56). This is in accordance with other studies that

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have demonstrated increases in plasma viscosity and hematocrit, regardless of whether fluid

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losses are replaced or not (83). Whereas, dehydration of 2% or less has minimal effects on

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whole blood viscosity (1) losses equal to 4-5%, which are common during exercise in warm

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conditions (77), have significant effects (1). Fluid shifts, water loss, water trapping in muscle,

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along with the aggregability and rigidity of red blood cells during hemoconcentration are all

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potential mechanisms for acute increases in blood viscosity in exercise (2, 9, 20).

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Maximal and submaximal exercise protocols of at least 20 minutes in duration have

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been shown to increase whole blood and plasma viscosity with corresponding increases in

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hematocrit and total protein concentrations (10, 11, 22, 48). Increases in plasma proteins such

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as fibrinogen and globulins, as well as water loss promote the increased plasma viscosity (14).

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These changes influence shear stress on the wall of the vessel and signal the release of nitric

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oxide and (14) and ROS release from the vessel wall (44). This occurs by activation of signaling

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pathways involving phosphatidylinositol-3-kinase, mitogen activated protein kinase 7, and nitric

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oxide (44). The subsequent oxidant production, if severe enough, can impair the rheological

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properties of blood (72) by increasing red blood cell rigidity and decreasing red blood cell

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deformability (3, 72). It is of note that endurance training appears to protect the red blood cell

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from oxidative damage (14).

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Shear stress not only induces ROS production but also is thought to be an important

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physical factor for hemolysis (46). Hemolysis can itself induce ROS formation via iron redox

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chemistry with ascorbate and O2. The degree of hemolysis, and presumably the amount of

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oxidative damage, is a result of the amplitude and exposure time to shear stress (7) and other

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factors such as the extent of dehydration (5). Thus, changes in blood rheology and shear stress

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emerge as two strong mechanistic factors that could account for some ROS production during

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dehydration associated with exercise.

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Osmotic stress in skeletal muscle fibers.

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Skeletal muscle fibers are exposed to a number of shifts in intracellular and extracellular

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solute content during exercise and during heat exposure that can result in osmotic stress. In

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general, intensely exercising muscles swell by surprising amounts, i.e. as much as 10-35% in

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fatiguing contractions, as reviewed in (81). In low load resistance exercise to exhaustion in

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humans (20% of maximum contraction), muscles swell by ≈20%, and remain swollen beyond 60

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min (90). Historically this was thought to be due to increases in interstitial or vascular fluid

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volume in the intact muscle tissue, but a number of exquisitely designed studies have brought a

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consensus that most of the swelling can be accounted for by osmotic gradients created across

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the muscle fibers, reviewed in (81). In theory, the swelling in exercising muscles could account

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for some of the loss of plasma volume and hemoconcentration seen in exercise, even during

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whole body euhydration (43). Importantly, metabolic water production must also be taken into

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account as ultimately all macronutrients undergo reactions with oxygen to produce carbon

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dioxide and water as a consequence of skeletal muscle metabolism during contraction. The total

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volume of water produced in the muscle is dependent upon the amount of substrate

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metabolized, but it is estimated that 0.6 ml of water is formed per gram of oxidized carbohydrate

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whereas 1.1 ml of water is generated per gram of oxidized fat (50). While this metabolic water

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production is usually neglected when hydration status is estimated from changes in body mass

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(50), it may account for some skeletal muscle fiber swelling during contraction.

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Osmotic changes are also responsible for the acute swelling in exercising muscle and

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include the splitting of phosphocreatine (PCr), elevations in lactate and H+ when arising from

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muscle glycogen, and shifts in ion gradients due to reductions in membrane potential (37, 81).

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Skeletal muscle PCr levels can be as high as 30 mM at rest. During volitional exercise to

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exhaustion, PCr can decrease by 70% (39). Therefore, just through PCr splitting alone, intense

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exercise can easily result in a >20 mOsm increase in intracellular solute concentration, which

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could account for ≈7% elevation in intracellular volume as water moves down its concentration

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gradient.

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Most cell types exposed to acute heat stress also undergo a period of rapid swelling (up

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to 40%) over an hour or more, but then exhibit a loss of cell volume over several hours (24, 32).

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Presumably, acute swelling occurs in skeletal muscle fibers during heat exposure, but to our

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knowledge this has not been directly studied. Cell or fiber volume increases in exercise or

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hyperthermia are normally countered to some extent by reductions in plasma volume of 3-9%

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(42). Conceivably, when the plasma is hypertonic in exercise, non-exercising muscles are

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exposed to opposing forces that tend to induce outward fluid movement, eliciting an entirely

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different set of stress signaling responses not discussed here, see (37).

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The ability of skeletal muscle to compensate for osmotic challenges due to swelling in

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exercise is dependent, in part, on localized ROS formation. Immediately following exposure to

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hypotonic extracellular fluid, experimentally causing muscle fibers to swell, there is a rapid and

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very localized production of ROS near the muscle membrane (49, 61), Fig. 2A. The resulting

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ROS signal has been linked to localized elevations in intracellular Ca2+ in mouse skeletal

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muscle, in the form of Ca2+ sparks (49). Both the ROS signal and the Ca2+ sparks can be

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inhibited by NADPH oxidase (NOX) inhibitors (49). ROS-activated pathways are important

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mediators of the regulation of muscle volume control because antioxidant treatments or NOX

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inhibitors block the Ca2+ signal (49) and impair compensatory osmolyte transport mechanisms

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discussed below, Fig. 2B (61). Localized ROS formation may not function as a specific mediator

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of osmotic stress adaptation, but rather it may operate by creating a locally oxidizing

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environment (pink region in Figure 1A) that induces generalized phosphatase inhibition and

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kinase facilitation, thus prolonging the half-life of phosphorylation events that drive the signaling

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responses to stress exposure (89).

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The current thinking regarding the physiology of the compensatory regulation to muscle cell

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swelling in heat or exercise and its relationship to ROS formation is summarized as follows and

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is outlined in Figure 2A:

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1. The mechanism by which muscle cells detect increases in fiber volume is unknown but

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the most likely mechanism involves activation of transient receptor potential channels

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(TRP), such as TRPV4 (84), which are present on muscle fibers and are sensitive to both

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mechanical stretch and eicosanoids derived from PLA2 activity (23, 37). In response to

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stimulation they elevate cation permeability, most importantly, the permeability to

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extracellular Ca2+. Interestingly, TPRV2 is required for normal skeletal muscle cell

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responses to reductions in cell volume (91) and TPR channels play important roles in

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thermal sensitivity throughout the body, as reviewed in (36).

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2. The elevation in subsarcolemmal Ca2+ may be responsible for supporting assembly and

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activation of Rac-activated NADPH oxidase, which is believed to be the primary source of

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ROS during osmotic stress (49). In addition, it may contribute to Ca2+-induced Ca2+

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release from the sarcoplasmic reticulum (SR). The combination of elevations in local

386

Ca2+, the local ROS formation, and in the distortions of the coupling of the SR to the cell

387

membrane or T tubules due to mechanical effects of swelling, are believed to contribute

388

to the formation of Ca2+ sparks at the SR in skeletal muscle (49).

389

3. The elevation in intracellular Ca2+ can also support activation of Ca2+-dependent

390

phospholipases (e.g. PLA2), which produce arachidonic acid and other eicosanoid

391

substrates. The phospholipases are also activated by heat (12) and in many ways may

392

be responsible for many overlapping pathways involved with cellular responses to cell

393

swelling, hyperthermia and exercise. Many different eicosanoid products have specific

394

and sometimes opposing effects on cellular osmoregulation (37), but in skeletal muscle

395

the most specific response identified so far are related to products from lipoxygenase-5

396

enzyme, LOX-5 (61). LOX-5 produces leukotriene B4 (LKB4) and many other biologically

397

active eicosanoids. Another important product of PLA2 is lysophosphatidyl choline

398

(LPC), which can lead to osmolyte release during osmotic stress, possibly by activation of

399

protein kinase C (PKC) (38, 61).

400

4. One of the questions regarding the mechanism for ROS production in heat has been why

401

lipoxygenase blockade results in such potent suppression of muscle ROS formation (92).

402

The answer may lie in the discovery that receptors for LOX5 products, such as the

403

leukotriene-B4 (LKB4) receptor (Fig. 2A) induce activation of the GTPase, Rac, which is

404

essential for assembly of NADPH oxidase (13, 87). Therefore, ROS production may

405

depend or be sustained by an autocrine/paracrine signaling pathway of eicosanoids

406

released during osmotic or heat stress (Fig. 2A). Understanding this pathway is complex

407

because each component of the system described in Fig. 2A augments the response of

408

upstream elements. Therefore, there is much uncertainty in defining where the cellular

409

response to osmotic stress starts, how the signaling elements are linked together and

410

how the response is terminated.

411

5. There are three primary response elements by which cells respond to swelling that

412

together are called the “regulatory volume decrease” (RVD) response (37). The

413

underlying strategy that all cells use is to pass substantial osmolytes out of the cell;

414

causing water to leave down its concentration gradient, and returning the cell volume to

415

near its normal set point. The primary osmolytes released are K+, anions (largely Cl- and

416

HCO3-) and the amino acid, taurine (37, 61, 81), Fig. 2B. Each pathway responds to a

417

number of signals generated by the osmotic sensing pathways described in Fig. 2A;

418

ROS, eicosanoids and Ca2+ are among the most important activators of these

419

transporters or channels and when blocked, suppress the ability of the fiber to regulate

420

volume.

421

6. Note that free taurine in skeletal muscle is very high; e.g. in human vastus lateralis

422

muscle it is 50 mM (6), about 1000 times that of plasma, and skeletal muscle abundantly

423

expresses taurine transporters (68). In response to exercise, ischemia and other

424

perturbations that cause muscles to swell, taurine is released very rapidly, thus returning

425

the muscle fiber to osmotic balance (63, 78). It may be one of the most important

426

mechanisms of maintaining volume control in skeletal muscle fibers. The release of

427

taurine is also dependent on activation of phospholipases as well as other downstream

428

pathways of arachidonic acid metabolism driven by PLA2 and lipoxygenase (61, 78).

429 430

These cellular responses to osmotic stress that are predicted to occur in both exercise and

431

hyperthermia are designed to prevent muscle cells from being damaged and are dependent on

432

the formation of localized ROS. Their existence provides insight into at another possible

433

mechanism by which exercise induces elevations in circulating ROS.

434 435 436

Summary and translational Implications The origin and importance of ROS in exercise remains a controversial topic. The general

437

discussion in the field of exercise physiology is most often restricted to whether ROS,

438

antioxidants or fluid replacement are “good or bad” for performance or for adaptations to

439

training. Considering the complexity of ROS actions throughout the body, it is not surprising that

440

experiments that try to block ROS during exercise often have very mixed and sometimes

441

unpredictable outcomes. Here we attempt to extend the framework of this discussion to

442

environmental factors that, based on solid experimental evidence, clearly are important in ROS

443

formation in exercise. The observations made here regarding the potential influence of these

444

environmental factors come from many sources and many kinds of exercise stimuli. This leads

445

to questions regarding how the nature of the exercise stimulus (e.g. resistance vs. endurance

446

exercise) and the duration of the exercise affect the unfolding of oxidant/antioxidant responses

447

in the circulation and how these link to the timing of alterations in osmotic stress, dehydration

448

and temperature in various tissues. Nevertheless, given the limitations of our understanding,

449

the schema in Fig 3 provides a visual summary of the pathways and mechanisms involved,

450

which may serve as a hypothesis generating tool for future studies, both for identifying possible

451

ROS forming mechanisms and for identifying candidates for intervention. Consider the scenario

452

of trying to avoid ROS produced in exercise with a cocktail of antioxidants. Clearly some ROS

453

pathways are necessary for normal muscle fluid volume homeostasis. Blocking this may result

454

in a greater potential for muscle injury. Likewise, some normal hemoconcentration may be

455

necessary to assist water movement out of exercising muscle in order to maintain cross bridge

456

lattice structure. On the other hand, strategies (e.g. hydration) to prevent excessive shear stress

457

or protecting red blood cells from hemolysis could result in positive consequences.

458

459

Acknowledgements:

460 461 462 463

TLC was supported in part by the BK and Betty Stevens Endowed Professorship at the University of Florida. OL was supported by a postdoctoral fellowship from the Brazilian Council for the Development of Science and Technology (CNPq #249094/2013-4) and by the Federal University of Vale do São Francisco - UNIVASF - Petrolina/PE.

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References

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1. Ahmadizad S, El-Sayed MS, and MacLaren DP. Effects of water intake on the responses of haemorheological variables to resistance exercise. Clin Hemorheol Microcirc 35: 317-327, 2006. 2. Ahmadizad S, Moradi A, Nikookheslat S, Ebrahimi H, Rahbaran A, and Connes P. Effects of age on hemorheological responses to acute endurance exercise. Clin Hemorheol Microcirc 49: 165-174, 2011. 3. Ajmani RS, Fleg JL, Demehin AA, Wright JG, O'Connor F, Heim JM, Tarien E, and Rifkind JM. Oxidative stress and hemorheological changes induced by acute treadmill exercise. Clin Hemorheol Microcirc 28: 29-40, 2003. 4. Anderson EJ, and Neufer PD. Type II skeletal myofibers possess unique properties that potentiate mitochondrial H(2)O(2) generation. Am J Physiol Cell Physiol 290: C844-851, 2006. 5. Bennekou P, de Franceschi L, Pedersen O, Lian L, Asakura T, Evans G, Brugnara C, and Christophersen P. Treatment with NS3623, a novel Cl-conductance blocker, ameliorates erythrocyte dehydration in transgenic SAD mice: a possible new therapeutic approach for sickle cell disease. Blood 97: 1451-1457, 2001. 6. Blomstrand E, and Saltin B. Effect of muscle glycogen on glucose, lactate and amino acid metabolism during exercise and recovery in human subjects. J Physiol 514 ( Pt 1): 293-302, 1999. 7. Boehning F, Mejia T, Schmitz-Rode T, and Steinseifer U. Hemolysis in a laminar flowthrough Couette shearing device: an experimental study. Artif Organs 38: 761-765, 2014. 8. Brooks GA, Hittelman KJ, Faulkner JA, and Beyer RE. Temperature, skeletal muscle mitochondrial functions, and oxygen debt. Am J Physiol 220: 1053-1059, 1971. 9. Brun JF, Khaled S, Raynaud E, Bouix D, Micallef JP, and Orsetti A. The triphasic effects of exercise on blood rheology: which relevance to physiology and pathophysiology? Clin Hemorheol Microcirc 19: 89-104, 1998. 10. Brun JF, Micallef JP, and Orsetti A. Hemorheologic Effects of Light Prolonged Exercise. Clin Hemorheol 14: 807-818, 1994. 11. Brun JF, Micallef JP, Supparo I, and Orsetti A. Effects of a Standardized Breakfast Compared to Fasting on the Hemorheologic Responses to Submaximal Exercise. Clin Hemorheol 15: 213-220, 1995. 12. Calderwood SK, Bornstein B, Farnum EK, and Stevenson MA. Heat shock stimulates the release of arachidonic acid and the synthesis of prostaglandins and leukotriene B4 in mammalian cells. J Cell Physiol 141: 325-333, 1989. 13. Cho KJ, Seo JM, and Kim JH. Bioactive lipoxygenase metabolites stimulation of NADPH oxidases and reactive oxygen species. Mol Cells 32: 1-5, 2011. 14. Connes P, Simmonds MJ, Brun JF, and Baskurt OK. Exercise hemorheology: classical data, recent findings and unresolved issues. Clin Hemorheol Microcirc 53: 187-199, 2013. 15. Cotgreave IA, Goldschmidt L, Tonkonogi M, and Svensson M. Differentiation-specific alterations to glutathione synthesis in and hormonally stimulated release from human skeletal muscle cells. FASEB J 16: 435-437, 2002. 16. Davies KJ, Quintanilha AT, Brooks GA, and Packer L. Free radicals and tissue damage produced by exercise. Biochem Biophys Res Commun 107: 1198-1205, 1982. 17. Di Meo S, and Venditti P. Mitochondria in exercise-induced oxidative stress. Biol Signals Recept 10: 125-140, 2001. 18. Dillard CJ, Litov RE, Savin WM, Dumelin EE, and Tappel AL. Effects of exercise, vitamin E, and ozone on pulmonary function and lipid peroxidation. J Appl Physiol Respir Environ Exerc Physiol 45: 927-932, 1978.

511 512 513 514 515 516 517 518 519 520 521 522 523 524 525 526 527 528 529 530 531 532 533 534 535 536 537 538 539 540 541 542 543 544 545 546 547 548 549 550 551 552 553 554 555 556 557 558

19. Durand MJ, Dharmashankar K, Bian JT, Das E, Vidovich M, Gutterman DD, and Phillips SA. Acute exertion elicits a H2O2-dependent vasodilator mechanism in the microvasculature of exercise-trained but not sedentary adults. Hypertension 65: 140-145, 2015. 20. El-Sayed MS. Effects of exercise and training on blood rheology. Sports Med 26: 281-292, 1998. 21. El-Sayed MS, Ali N, and El-Sayed Ali Z. Haemorheology in exercise and training. Sports Med 35: 649-670, 2005. 22. Ernst E, Daburger L, and Saradeth T. The Kinetics of Blood Rheology during and after Prolonged Standardized Exercise. Clin Hemorheol 11: 429-439, 1991. 23. Gailly P. TRP channels in normal and dystrophic skeletal muscle. Curr Opin Pharmacol 12: 326-334, 2012. 24. Gervais P, Martı́nez de Marañón I, Evrard C, Ferret E, and Moundanga S. Cell volume changes during rapid temperature shifts. Journal of Biotechnology 102: 269-279, 2003. 25. Gil SM, Yazaki E, and Evans DF. Aetiology of running-related gastrointestinal dysfunction. How far is the finishing line? Sports Med 26: 365-378, 1998. 26. Golub AS, and Pittman RN. Bang-bang model for regulation of local blood flow. Microcirculation 20: 455-483, 2013. 27. Gong MC, Arbogast S, Guo Z, Mathenia J, Su W, and Reid MB. Calcium-independent phospholipase A2 modulates cytosolic oxidant activity and contractile function in murine skeletal muscle cells. J Appl Physiol (1985) 100: 399-405, 2006. 28. Gonzalez-Alonso J, Crandall CG, and Johnson JM. The cardiovascular challenge of exercising in the heat. J Physiol 586: 45-53, 2008. 29. Hall DM, Buettner GR, Matthes RD, and Gisolfi CV. Hyperthermia stimulates nitric oxide formation: electron paramagnetic resonance detection of .NO-heme in blood. J Appl Physiol (1985) 77: 548-553, 1994. 30. Hall DM, Buettner GR, Oberley LW, Xu L, Matthes RD, and Gisolfi CV. Mechanisms of circulatory and intestinal barrier dysfunction during whole body hyperthermia. Am J Physiol Heart Circ Physiol 280: H509-521, 2001. 31. Hass MA, and Massaro D. Regulation of the synthesis of superoxide dismutases in rat lungs during oxidant and hyperthermic stresses. J Biol Chem 263: 776-781, 1988. 32. Herman TS, Gerner EW, Magun BE, Stickney D, Sweets CC, and White DM. Rate of heating as a determinant of hyperthermic cytotoxicity. Cancer Res 41: 3519-3523, 1981. 33. Herrero A, and Barja G. ADP-regulation of mitochondrial free radical production is different with complex I- or complex II-linked substrates: implications for the exercise paradox and brain hypermetabolism. J Bioenerg Biomembr 29: 241-249, 1997. 34. Hillman AR, Turner MC, Peart DJ, Bray JW, Taylor L, McNaughton LR, and Siegler JC. A comparison of hyperhydration versus ad libitum fluid intake strategies on measures of oxidative stress, thermoregulation, and performance. Res Sports Med 21: 305-317, 2013. 35. Hillman AR, Vince RV, Taylor L, McNaughton L, Mitchell N, and Siegler J. Exerciseinduced dehydration with and without environmental heat stress results in increased oxidative stress. Appl Physiol Nutr Metab 36: 698-706, 2011. 36. Hilton JK, Rath P, Helsell CV, Beckstein O, and Van Horn WD. Understanding thermosensitive transient receptor potential channels as versatile polymodal cellular sensors. Biochemistry 54: 2401-2413, 2015. 37. Hoffmann EK, Lambert IH, and Pedersen SF. Physiology of cell volume regulation in vertebrates. Physiol Rev 89: 193-277, 2009. 38. Jackson MJ, Edwards RH, and Symons MC. Electron spin resonance studies of intact mammalian skeletal muscle. Biochim Biophys Acta 847: 185-190, 1985.

559 560 561 562 563 564 565 566 567 568 569 570 571 572 573 574 575 576 577 578 579 580 581 582 583 584 585 586 587 588 589 590 591 592 593 594 595 596 597 598 599 600 601 602 603 604 605 606 607 608

39. Jones AM, Wilkerson DP, Berger NJ, and Fulford J. Influence of endurance training on muscle [PCr] kinetics during high-intensity exercise. Am J Physiol Regul Integr Comp Physiol 293: R392-401, 2007. 40. Kadenbach B, Ramzan R, Wen L, and Vogt S. New extension of the Mitchell Theory for oxidative phosphorylation in mitochondria of living organisms. Biochim Biophys Acta 1800: 205212, 2010. 41. Kikusato M, and Toyomizu M. Crucial role of membrane potential in heat stress-induced overproduction of reactive oxygen species in avian skeletal muscle mitochondria. PLoS One 8: e64412, 2013. 42. Laitano O, Kalsi KK, Pearson J, Lotlikar M, Reischak-Oliveira A, and Gonzalez-Alonso J. Effects of graded exercise-induced dehydration and rehydration on circulatory markers of oxidative stress across the resting and exercising human leg. European Journal of Applied Physiology 112: 1937-1944, 2012. 43. Laitano O, Kalsi KK, Pook M, Oliveira AR, and Gonzalez-Alonso J. Separate and combined effects of heat stress and exercise on circulatory markers of oxidative stress in euhydrated humans. European Journal of Applied Physiology 110: 953-960, 2010. 44. Lehoux S. Redox signalling in vascular responses to shear and stretch. Cardiovasc Res 71: 269-279, 2006. 45. Letcher RL, Pickering TG, Chien S, and Laragh JH. Effects of Exercise on Plasma Viscosity in Athletes and Sedentary Normal Subjects. Clin Cardiol 4: 172-179, 1981. 46. Leverett LB, Hellums JD, Alfrey CP, and Lynch EC. Red blood cell damage by shear stress. Biophys J 12: 257-273, 1972. 47. Loven DP, Leeper DB, and Oberley LW. Superoxide dismutase levels in Chinese hamster ovary cells and ovarian carcinoma cells after hyperthermia or exposure to cycloheximide. Cancer Res 45: 3029-3033, 1985. 48. Martin DG, Ferguson EW, Wigutoff S, Gawne T, and Schoomaker EB. Blood viscosity responses to maximal exercise in endurance-trained and sedentary female subjects. J Appl Physiol (1985) 59: 348-353, 1985. 49. Martins AS, Shkryl VM, Nowycky MC, and Shirokova N. Reactive oxygen species contribute to Ca2+ signals produced by osmotic stress in mouse skeletal muscle fibres. J Physiol 586: 197-210, 2008. 50. Maughan RJ, Shirreffs SM, and Leiper JB. Errors in the estimation of hydration status from changes in body mass. J Sports Sci 25: 797-804, 2007. 51. McAnulty SR, McAnulty L, Pascoe DD, Gropper SS, Keith RE, Morrow JD, and Gladden LB. Hyperthermia increases exercise-induced oxidative stress. Int J Sports Med 26: 188-192, 2005. 52. Mestre-Alfaro A, Ferrer MD, Banquells M, Riera J, Drobnic F, Sureda A, Tur JA, and Pons A. Body temperature modulates the antioxidant and acute immune responses to exercise. Free Radic Res 46: 799-808, 2012. 53. Montilla SIR, Johnson TP, Pearce SC, Gardan-Salmon D, Gabler NK, Ross JW, Rhoads RP, Baumgard LH, Lonergan SM, and Selsby JT. Heat stress causes oxidative stress but not inflammatory signaling in porcine skeletal muscle. Temperature 1: 42-50, 2014. 54. Murphy MP. How mitochondria produce reactive oxygen species. Biochemical Journal 417: 1-13, 2009. 55. Nethery D, Stofan D, Callahan L, DiMarco A, and Supinski G. Formation of reactive oxygen species by the contracting diaphragm is PLA(2) dependent. J Appl Physiol (1985) 87: 792800, 1999. 56. Ohira Y, Ito A, and Ikawa S. Hemoconcentration during isotonic handgrip exercise. J Appl Physiol Respir Environ Exerc Physiol 42: 744-745, 1977. 57. Ohtsuka Y, Yabunaka N, Fujisawa H, Watanabe I, and Agishi Y. Effect of thermal stress on glutathione metabolism in human erythrocytes. Eur J Appl Physiol Occup Physiol 68: 87-91, 1994.

609 610 611 612 613 614 615 616 617 618 619 620 621 622 623 624 625 626 627 628 629 630 631 632 633 634 635 636 637 638 639 640 641 642 643 644 645 646 647 648 649 650 651 652 653 654 655 656 657

58. Oliver SR, Phillips NA, Novosad VL, Bakos MP, Talbert EE, and Clanton TL. Hyperthermia induces injury to the intestinal mucosa in the mouse: evidence for an oxidative stress mechanism. Am J Physiol Regul Integr Comp Physiol 302: R845-853, 2012. 59. Oliver SR, Wright VP, Parinandi N, and Clanton TL. Thermal tolerance of contractile function in oxidative skeletal muscle: no protection by antioxidants and reduced tolerance with eicosanoid enzyme inhibition. Am J Physiol Regul Integr Comp Physiol 295: R1695-1705, 2008. 60. Omar RA, Yano S, and Kikkawa Y. Antioxidant enzymes and survival of normal and simian virus 40-transformed mouse embryo cells after hyperthermia. Cancer Res 47: 3473-3476, 1987. 61. Ortenblad N, Young JF, Oksbjerg N, Nielsen JH, and Lambert IH. Reactive oxygen species are important mediators of taurine release from skeletal muscle cells. Am J Physiol Cell Physiol 284: C1362-1373, 2003. 62. Paik IY, Jeong MH, Jin HE, Kim YI, Suh AR, Cho SY, Roh HT, Jin CH, and Suh SH. Fluid replacement following dehydration reduces oxidative stress during recovery. Biochem Biophys Res Commun 383: 103-107, 2009. 63. Parkin JM, Carey MF, Zhao S, and Febbraio MA. Effect of ambient temperature on human skeletal muscle metabolism during fatiguing submaximal exercise. J Appl Physiol (1985) 86: 902908, 1999. 64. Pilch W, Szygula Z, Tyka AK, Palka T, Tyka A, Cison T, Pilch P, and Teleglow A. Disturbances in pro-oxidant-antioxidant balance after passive body overheating and after exercise in elevated ambient temperatures in athletes and untrained men. PLoS One 9: e85320, 2014. 65. Powers SK, Talbert EE, and Adhihetty PJ. Reactive oxygen and nitrogen species as intracellular signals in skeletal muscle. J Physiol 589: 2129-2138, 2011. 66. Quarrie R, Lee DS, Reyes L, Erdahl W, Pfeiffer DR, Zweier JL, and Crestanello JA. Mitochondrial uncoupling does not decrease reactive oxygen species production after ischemiareperfusion. Am J Physiol Heart Circ Physiol 307: H996-H1004, 2014. 67. Quindry J, Miller L, McGinnis G, Kliszczewiscz B, Slivka D, Dumke C, Cuddy J, and Ruby B. Environmental temperature and exercise-induced blood oxidative stress. Int J Sport Nutr Exerc Metab 23: 128-136, 2013. 68. Ramamoorthy S, Leibach FH, Mahesh VB, Han H, Yang-Feng T, Blakely RD, and Ganapathy V. Functional characterization and chromosomal localization of a cloned taurine transporter from human placenta. Biochem J 300 ( Pt 3): 893-900, 1994. 69. Ramanadham S, Ali T, Ashley JW, Bone RN, Hancock WD, and Lei X. CalciumIndependent Phospholipases A2 (iPLA2s) and their Roles in Biological Processes and Diseases. J Lipid Res 2015. 70. Reid MB, Haack KE, Franchek KM, Valberg PA, Kobzik L, and West MS. Reactive oxygen in skeletal muscle. I. Intracellular oxidant kinetics and fatigue in vitro. J Appl Physiol (1985) 73: 1797-1804, 1992. 71. Reid MB, Shoji T, Moody MR, and Entman ML. Reactive oxygen in skeletal muscle. II. Extracellular release of free radicals. J Appl Physiol (1985) 73: 1805-1809, 1992. 72. Rifkind JM, Ajmani RS, and Heim J. Impaired hemorheology in the aged associated with oxidative stress. Adv Exp Med Biol 428: 7-13, 1997. 73. Ristow M, Zarse K, Oberbach A, Kloting N, Birringer M, Kiehntopf M, Stumvoll M, Kahn CR, and Bluher M. Antioxidants prevent health-promoting effects of physical exercise in humans. Proc Natl Acad Sci U S A 106: 8665-8670, 2009. 74. Ryan AJ, Gisolfi CV, and Moseley PL. Synthesis of 70K stress protein by human leukocytes: effect of exercise in the heat. J Appl Physiol (1985) 70: 466-471, 1991. 75. Salo DC, Donovan CM, and Davies KJ. HSP70 and other possible heat shock or oxidative stress proteins are induced in skeletal muscle, heart, and liver during exercise. Free Radic Biol Med 11: 239-246, 1991.

658 659 660 661 662 663 664 665 666 667 668 669 670 671 672 673 674 675 676 677 678 679 680 681 682 683 684 685 686 687 688 689 690 691 692 693 694 695 696 697 698 699 700 701 702 703 704 705 706 707

76. Sawka MN, and Noakes TD. Does dehydration impair exercise performance? Med Sci Sports Exerc 39: 1209-1217, 2007. 77. Senay LC, Jr., and Pivarnik JM. Fluid shifts during exercise. Exerc Sport Sci Rev 13: 335387, 1985. 78. Stutzin A, Torres R, Oporto M, Pacheco P, Eguiguren AL, Cid LP, and Sepulveda FV. Separate taurine and chloride efflux pathways activated during regulatory volume decrease. Am J Physiol 277: C392-402, 1999. 79. Sureda A, Ferrer MD, Tauler P, Romaguera D, Drobnic F, Pujol P, Tur JA, and Pons A. Effects of exercise intensity on lymphocyte H2O2 production and antioxidant defences in soccer players. Brit J Sport Med 43: 186-190, 2009. 80. Sureda A, Mestre-Alfaro A, Banquells M, Riera J, Drobnic F, Camps J, Joven J, Tur JA, and Pons A. Exercise in a hot environment influences plasma anti-inflammatory and antioxidant status in well-trained athletes. J Therm Biol 47: 91-98, 2015. 81. Usher-Smith JA, Huang CL, and Fraser JA. Control of cell volume in skeletal muscle. Biol Rev Camb Philos Soc 84: 143-159, 2009. 82. van der Poel C, and Stephenson DG. Effects of elevated physiological temperatures on sarcoplasmic reticulum function in mechanically skinned muscle fibers of the rat. Am J Physiol Cell Physiol 293: C133-141, 2007. 83. Vandewalle H, Lacombe C, Lelievre JC, and Poirot C. Blood viscosity after a 1-h submaximal exercise with and without drinking. Int J Sports Med 9: 104-107, 1988. 84. Vriens J, Watanabe H, Janssens A, Droogmans G, Voets T, and Nilius B. Cell swelling, heat, and chemical agonists use distinct pathways for the activation of the cation channel TRPV4. Proc Natl Acad Sci U S A 101: 396-401, 2004. 85. Welc SS, Phillips NA, Oca-Cossio J, Wallet SM, Chen DL, and Clanton TL. Hyperthermia increases interleukin-6 in mouse skeletal muscle. Am J Physiol Cell Physiol 303: C455-466, 2012. 86. Wendt D, van Loon LJ, and Lichtenbelt WD. Thermoregulation during exercise in the heat: strategies for maintaining health and performance. Sports Med 37: 669-682, 2007. 87. Woo CH, You HJ, Cho SH, Eom YW, Chun JS, Yoo YJ, and Kim JH. Leukotriene B(4) stimulates Rac-ERK cascade to generate reactive oxygen species that mediates chemotaxis. J Biol Chem 277: 8572-8578, 2002. 88. Wood SC, Doyle MP, and Appenzeller O. Effects of endurance training and long distance running on blood viscosity. Med Sci Sports Exerc 23: 1265-1269, 1991. 89. Wright VP, Reiser PJ, and Clanton TL. Redox modulation of global phosphatase activity and protein phosphorylation in intact skeletal muscle. J Physiol 587: 5767-5781, 2009. 90. Yasuda T, Fukumura K, Iida H, and Nakajima T. Effect of low-load resistance exercise with and without blood flow restriction to volitional fatigue on muscle swelling. Eur J Appl Physiol 115: 919-926, 2015. 91. Zanou N, Mondin L, Fuster C, Seghers F, Dufour I, de Clippele M, Schakman O, Tajeddine N, Iwata Y, Wakabayashi S, Voets T, Allard B, and Gailly P. Osmosensation in TRPV2 dominant negative expressing skeletal muscle fibres. J Physiol 593: 3849-3863, 2015. 92. Zuo L, Christofi FL, Wright VP, Bao S, and Clanton TL. Lipoxygenase-dependent superoxide release in skeletal muscle. J Appl Physiol (1985) 97: 661-668, 2004. 93. Zuo L, Christofi FL, Wright VP, Liu CY, Merola AJ, Berliner LJ, and Clanton TL. Intra- and extracellular measurement of reactive oxygen species produced during heat stress in diaphragm muscle. Am J Physiol Cell Physiol 279: C1058-1066, 2000. 94. Zuo L, Pasniciuc S, Wright VP, Merola AJ, and Clanton TL. Sources for superoxide release: lessons from blockade of electron transport, NADPH oxidase, and anion channels in diaphragm. Antioxid Redox Signal 5: 667-675, 2003.

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Figure 1. Summary of the findings in the “acute” plasma responses considering the results of the human studies reported in this review. Note that hyperthermia alone increases ROS formation and decreases antioxidant defense while hyperthermia combined with exercise increases ROS formation and antioxidant defense. Dehydration combined with exercise has a similar response to hyperthermia alone and rehydration can maintain ROS formation while increasing antioxidant defense.

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Figure 2. A. Signaling pathways involved in ROS formation during osmotic stress and/or hyperthermia in skeletal muscle. Stretch or solute concentration are sensed at the cell membrane by a poorly understood sensing system, most likely an isoform of transient receptor potential channels (TRP) that function as stress activated Ca+2 channels. The elevation in Ca+2or other signaling systems can induce ROS formation by stimulation of NADP oxidase activity and by activation of phospholipase (PLA2). Elevations in PLA2 support both protein kinase C (PKC) activation and eicosanoid metabolism such as via lipoxygenase. Lipoxygenase activity supports ROS formation either directly or through autocrine/paracrine signaling to eicosanoid receptors, such as leukotriene B4 receptor, which can activate the GTPase, Rac, the primary signaling pathway for NADPH oxidase. B. Ca+2, ROS, eicosanoid activity, stretch, PKC activity and other signals play integrative roles in opening K+ and anion channels (Cl-) and in activating taurine transport mechanisms. Taurine transport may be of unique importance in skeletal muscle osmotic homeostasis and is dependent of ROS formed during osmotic stress for its activation.

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Figure 3. Working model for sources of ROS during exercise, dehydration or hyperthermia. Both hyperthermia and exercise, alone or in combination, can induce both muscle fiber swelling and whole body evaporative water losses. Both processes contribute to hemoconcentration, which can elevate ROS through changes in blood viscosity, associated sheer stress and hemolysis. Muscle fiber swelling, caused by elevations in solute content in muscle fibers during exercise induces the “regulatory volume decrease” response (RVD), which is dependent on both localized ROS signaling and on eicosanoid metabolism through phospholipase A2 and downstream arachidonic acid (AA) metabolism pathways, eventually leading to ROS formation through NOX. Hyperthermia independently activates PLA2 and produces ROS from either NOX pathways or from mitochondrial sources. Finally, the RVD response functions to return fiber volume to steady state by activation of solute transport systems that push solutes out of the muscle fiber. This includes the taurine transport system.

745

746

Table 1. Studies performed in humans on the effects of hyperthermia on exercise-induced oxidative stress. Author/year of publication

Hyperthermia

Ohtsuka et al. 1994 (57)

Body temperature not reported

Erythrocyte Plasma

Glutathione, glutathione peroxidase, glutathione reductase, lipid peroxydes.

10 min of hot bath immersion

McAnulty et al. 2005 (51)

39.5˚C rectal temperature

Plasma

Isoprostanes, lipid hydroperoxides.

~50 min of walking/running inside a climatic chamber at 50% VO2max

Hyperthermia increases oxidative stress independent of oxygen consumption

Mestre-Alfaro et al. 2012 (52)

39.4°C rectal temperature and 36.9°C skin temperature

Blood

Malondialdehyde, protein carbonyls, glutathione reductase, glutathione peroxidase

45 minutes at 75-80% VO2 max at either 10-12°C or 3032°C

Leukocyte and neutrophil antioxidant enzyme activity increased and carbonyl index decreased following exercise in hot environment

38.3˚C rectal temperature

Whole blood, plasma, erythrocytes

Glutathione, glutathione disulfide, superoxide dismutase, isoprostantes.

~75min of heat protocol with water perfused suit followed by 5 min isolated knee extensor exercise

Resting heat stress induces non-radical oxidative stress, but moderate exercise negates the effects of heat through anti-oxidant compensation

39.2˚C rectal temperature

Blood plasma

Lipid hydroperoxide, protein carbonyls, trolox equivalent, ascorbate equivalent

60 min of exercise at 33°C

Increased oxidative stress in blood following moderate exercise and recovery in a warm environment

1.2˚C increase in rectal temperature

Blood plasma

Antioxidant status, peroxidation products

Cycle ergometer at 50% VO2max until hyperthermia vs passive heating in sauna

Passive heating caused a greater increase in oxidative stress indices when compared to physical exercise in elevated ambient temperatures

39.8 °C rectal temperature

Blood plasma

Malondialdehyde, protein carbonyls, nucleic acid oxidation, enzymatic activity of catalase and superoxide dismutase.

45 minutes at 75-80% VO2 max at either 10-12°C or 3032°C

Hot environmental temperatures induced greater oxidative and cellular damage but also increased post exercise increases in antioxidants

Laitano et al. 2010 (43)

Tissue

Biomarkers

38.7˚C Skin temperature Quindry et al. 2013 (67)

Pilch et al. 2014 (64)

Sureda et al. 2015 (80)

Protocols

Results/Conclusion

Heat stress causes oxidative stress and cold stress increases antioxidant defenses

747 748

Table 2. Studies performed in humans on the effects of dehydration on exercise-induced oxidative stress. Author/year of publication

Dehydration (change in body mass)

Tissue

Biomarkers

Protocols

Results/Conclusion

3%

Blood plasma

Malondialdehyde, total antioxidant status, limphocyte DNA damage

3 h sauna followed by treadmill running to exhaustion at 80% of VO2max

Dehydration increases DNA damage during exercise to exhaustion and fluid replacement decreases DNA damage

Hillman et al. 2011 (35)

3.8%

Whole blood Plasma Monocyte Lymphocyte

Glutathione, glutathione disulfide, TBARS, HSP72, HSP32

90 min of cycling at 95% of LT* followed by 5 km time trial

Exercise induced dehydration increased GSSG concentration and euhydration prevented increases regardless of environment

Laitano et al. 2012 (42)

2% and 3.5%

Whole blood Plasma Erythrocytes

Glutthione, glutathione disulfide, superoxide dismutase, isoprostanes

60 min cycling in the heat followed by one-leg knee extensor exercise

Mild and moderate dehydration decreased femoral venous erythrocyte SOD activity

Paik et al. 2009 (62)

749 750

*LT = lactate threshold, HSP = heat shock protein, SOD = superoxide dismutase, GSSG = glutathione disulfide

A. Signaling pathways In fiber swelling

B. Osmolyte movements in the regulatory response to cell swelling

EXERCISE + HYPERTHERMIA

 Evaporative water loss + Sweating

Muscle fiber swelling

 Hemoconcentration

Increased blood viscosity

 Muscle fiber-”Regulatory Volume Decrease”

 PLA

2

AA

LOX-5

Increased vascular shear stress Hemolysis Fe+2

ROS



 ROS

LKB4…

 NOX ROS Solute transport e.g. (taurine)



 Hyperthermia

Mitochondria