medium containing M-CSF (25 ng/ml). RANKL (50 ng/ ml) every 3â4 d. On d 11â14, osteoclasts were treated with. ANGPTL4 (1â100 ng/ml), OPG (500 ng/ml), ...
The FASEB Journal • Research Communication
Hypoxia-inducible factor regulates osteoclast-mediated bone resorption: role of angiopoietin-like 4 Helen J. Knowles,*,1 Anne-Marie Cleton-Jansen,† Eberhard Korsching,‡ and Nicholas A. Athanasou§ *Botnar Research Centre and §Department of Pathology, Nuffield Department of Orthopaedics, Rheumatology, and Musculoskeletal Sciences, University of Oxford, Nuffield Orthopaedic Centre, Oxford, UK; †Department of Pathology, Leiden University Medical Center, Leiden, The Netherlands; and ‡Institute of Pathology, University of Muenster, Muenster, Germany Hypoxia and the hypoxia-inducible factor (HIF) transcription factor regulate angiogenic-osteogenic coupling and osteoclast-mediated bone resorption. To determine how HIF might coordinate osteoclast and osteoblast function, we studied angiopoietin-like 4 (ANGPTL4), the top HIF target gene in an Illumina HumanWG-6 v3.0 48k array of normoxic vs. hypoxic osteoclasts differentiated from human CD14ⴙ monocytes (14.3-fold induction, P
