Treatment of cells by ethidium bromide impairs complexes possessing mtDNA- encoded subunits. Q. Q. Q. I. IV. 1/2O2. H2O. NADH. NAD+. II. Succ. Fum. III. H+.
A
B A15
ϴ° sensitive element HN
O
rosamine O
N
CH3
II
Q III Q III IV Q
I
Fum Succ
MitoThermoYellow
Q
I
Fum Succ
The different targets of the respiratory chain inhibitors
Aa
IV Q
NADH
II
V
H2O 1/2O2
ATP
ADP Pi
Q III Q III IV Q Rot
F
H+
Overexpression of UCP1 results in oligomycin-resistant respiration
H+
H+
Q
Q III IV AOX Q II
H2O
V
H2O NADH 1/2O2 ADP ATP KCN Pi + NAD+ Pyr Pyr cyanohydrin COOH-CO-CH3 COOH-CNOH-CH3
Fum Succ
Overexpression of AOX results in propylgallate sensitive cyanide-resistant respiration
1/2O2
Oligo
I
E
PG
ADP Pi
ATP
D
NAD+
Fum Succ
H2O
NAD+
CH3
Treatment of cells by ethidium bromide impairs complexes possessing mtDNAencoded subunits
II II
V
½O2
NADH
C
Q
H+
NH 2
rhodamine 123
COOCH3
HN
H+
heat
I
H2O
1/2O2
NADH NAD+
III IV
III
V ATP ADP Pi
II Fum Succ
H+
I NADH NAD+
H+
Q
Q
UCP1
N
The mitochondrial respiratory chain concurrently generates ATP and heat
