Feb 6, 2004 - any treatment (day 0); for group 2, the mice were inoculated ...... L. Issel-Tarver, A. Kasarskis, S. Lewis, J. C. Matese, J. E. Richardson, M.
JOURNAL OF VIROLOGY, Oct. 2004, p. 11051–11060 0022-538X/04/$08.00⫹0 DOI: 10.1128/JVI.78.20.11051–11060.2004 Copyright © 2004, American Society for Microbiology. All Rights Reserved.
Vol. 78, No. 20
Identification of Differentially Expressed Genes in Scrapie-Infected Mouse Brains by Using Global Gene Expression Technology Wei Xiang,1 Otto Windl,1† Gerda Wu ¨nsch,1 Martin Dugas,2 Alexander Kohlmann,3 1 Nicola Dierkes, Ingo M. Westner,1 and Hans A. Kretzschmar1* Institute of Neuropathology,1 Department of Medical Informatics, Biometrics, and Epidemiology,2 and Laboratory for Leukemia Diagnostics, Department of Internal Medicine III, University Hospital Grosshadern,3 Ludwig-Maximilians-University Munich, Munich, Germany Received 6 February 2004/Accepted 14 June 2004
The pathogenesis of prion diseases, a class of transmissible fatal neurodegenerative diseases in humans and animals, is still unclear. The aim of this study was to identify the differentially regulated genes that correlate with the development of prion diseases for a better understanding of their pathological mechanisms. We employed Affymetrix Mouse Expression Arrays 430A containing >22,000 transcripts and compared the global gene expression profiles from brains of mice who were intracerebrally inoculated with scrapie strains ME7 and RML with those from brains of uninfected and mock-infected mice. The microarray data were analyzed by Significance Analysis of Microarrays, revealing 121 genes whose expression increased at least twofold in both ME7- and RML-infected mouse brains, with an estimated false discovery rate of
