Identification of regulatory targets of tissue-specific transcription factors ...
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Identification of regulatory targets of tissue-specific transcription factors ...
cThe genes selected for experimental validation are in bold font. dThis gene was not selected as positive control in our analysis, but has been found to be CRX ...
Figure S1. Polyadenylation sites in the 3’UTR of RPB2. Shown is the DNA sequence of the 3’UTR of RPB2, starting from the stop codon of the gene. The two polyadenylation sites are determined by RACE assays and noted. Figure S2. Peroxide and MMS do not induce the long RPB2 mRNA. A. Northern blot images showing the RPB2 mRNA in wild type cells (MVY150) after the cells are treated with 0mM, 1mM, 5mM, and 10mM H2O2 for 30 minutes. B. Similar to A except that MMS was used to treat the cells.
Figure S1. RPB2 3’UTR: 1
GAAATAATG T TGTA ACTCTA AGAAA GTAAA ATAATA AAAA
41 81
ACATTTCAT T TGTG CTGATC TTGCC AAAAA AAGGGT AAAC Poly(A) site 1 AAGTAGGCA A TAGT AATAAA ATAAG AATGA AGCATT TCTT
121
TTAATAAGC A GAGA GAACTT TCAAG CATTC AAAAAA CACA
161
AAAATAATG A TGAA TAAAAT GGGAC TCACA GTGTTT ATTT
201
ATAGTCCTT G TTTC GCATTT TACCA AATTA AAATTT AATT
241
TTATCAAGA C ATAT TAATGA ACTTT AGCGG AAAACC TTAA
281
ATATCCAAG T CATA AATAAT GACTT ATGTA AAAAGT TATA
321
AGAAAAATA T ACGG TGTTCA TTTTG GAACA A TGTTG CTGT Poly(A) site 2
