R&D Systems Tools for Cell Biology Research™
IHC Products & Protocol Guide
IHC & ICC cover images using R&D Systems primary antibodies. Delta-like 1 (DLL1) in Embryonic Rat Stomach
Wnt-2 in Human Stomach Cancer
Tie-2 in Embryonic Zebrafish
Catalog # AF3970
Catalog # AF3464
Catalog # AF928
Vanilloid R1 in Rat Dorsal Root Ganglion
Olig2 and Oligodendrocyte Marker O4 in Rat Cortical Stem Cells (RCSC)
IL-2 Receptor a in Mouse Spleen
Catalog # AF3066
Catalog # AF2418/MAB1326
Catalog # AF2438
Notch-2 in Rat Choroid Plexis
STAT6 in Human Peripheral Blood Mononuclear Cells (PBMCs)
Glutathione Peroxidase 1 (GPX1) in Rat Liver
Catalog # AF1190
Catalog # MAB2167
Catalog # AF3798
MESDC2 in Adult Mouse Testis
Meprin b Subunit in Mouse Intestine
GFAP in Rat Astrocytes
Catalog # AF4545
Catalog # AF3300
Catalog # AF2594
To view our continuously updated IHC/ICC image gallery, please visit our website at www.RnDSystems.com/go/IHCimages
TABLE OF CONTENTS This guide provides an introduction to the techniques, protocols, and troubleshooting methods that lead to a successful immunohistochemistry (IHC) or immunocytochemistry (ICC) experiment. Contributors include scientists from R&D Systems IHC/ICC core facility who have experience qualifying thousands of antibodies for these applications, and R&D Systems expert technical service team who have guided researchers through a range of IHC/ICC-related issues.
INTRODUCTION. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-10
IHC/ICC PROTOCOLS. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11-20
Designing a Successful IHC/ICC Experiment. . . . . . . . . . . . . . . . . . . . . . . . . . . 2
A. Making a 4% Formaldehyde Solution in PBS . . . . . . . . . . . . . . . . . . . . . 12
Sample Preparation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Paraffin-embedded & Frozen Tissues Cell Samples
B. Preparation of Gelatin-coated Slides for Histological Tissue Sections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Fixation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Formaldehyde, Alcohols, & Acetone Fixation of Tissues Fixation of Cultured Cells Preventing Non-specific Staining. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Preventing Non-specific Hydrophobic Interactions Preventing Non-specific Ionic Interactions Endogenous Enzyme Interference Endogenous Biotin Interference Primary Antibody Selection & Optimization. . . . . . . . . . . . . . . . . . . . . . . . . . 6 Selecting a Primary Antibody Supplier Selecting a Monoclonal or Polyclonal Antibody Affinity Purification of Polyclonal Antibodies Optimization of Primary Antibody Incubation Detection & Visualization. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7-8 Direct or Indirect Detection, & Amplification Fluorescence Detection Chromogenic Detection
C. Chromogenic Staining of Tissue Sections Using HRP-DAB Detection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14-15 Slide Preparation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Chromogenic Staining Using HRP-DAB Detection . . . . . . . . . . . . . . . . . 15 D. IHC Staining of Tissue Sections Using Fluorescence Microscopy. . . . . . 16 E. Antigen Retrieval Using Heat-induced Epitope Retrieval (HIER) . . . . . 17 F. Preparation of Coverslips & Cell Fixation for ICC. . . . . . . . . . . . . . . . . . . 18 G. Fluorescent ICC Staining of Cultured Cells. . . . . . . . . . . . . . . . . . . . . . . . 19 H. Preparation of a Cell Smear for Non-adherent Cell ICC. . . . . . . . . . . . . . 20 TROUBLESHOOTING GUIDE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21 ALPHABETICAL LISTING OF R&D SYSTEMS IHC/ICC ANTIBODIES. . . . . . . . . . . . . . . . . . . . . . . . 22-108 SUPPLEMENTAL IHC/ICC REAGENTS. . . . . . . . . . . . . . . . . . . . . . . . . . 109-112
IHC/ICC Controls. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9 Endogenous Tissue Background Control No Primary Antibody Control Isotype Control Absorption Control Tissue Type Control Limitations of Western Blot Comparisons Antigen Retrieval . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10 Antigen Retrieval Techniques Protease-induced Epitope Retrieval (PIER) Heat-induced Epitope Retrieval (HIER) Optimization & Limitations
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INTRODUCTION Designing a Successful IHC/ICC Experiment Immunohistochemistry (IHC) and immunocytochemistry (ICC) are techniques employed to localize antigen expression and are dependent on specific epitopeantibody interactions. IHC refers to the use of tissue sections, whereas ICC describes the use of cultured cells or cell suspensions. In both methods, positive staining is visualized using a molecular label, which can be fluorescent or chromogenic. Briefly, samples are fixed to preserve cellular integrity, and then subjected to incubation with blocking reagents to prevent non-specific binding of the antibodies. Samples are subsequently incubated with primary and secondary antibodies, and the signal is visualized for microscopic analysis. Technically, IHC/ICC are relatively simple and straightforward experimental methods. However, there are many variables which must be recognized and optimized for each individual IHC/ICC study. The most challenging aspect of these techniques is determining the experimental conditions necessary to generate a strong and specific signal for each antigen of interest. For example, detection of an abundant protein in cultured cells may require a short fixation period, minimal blocking, and may be compatible with direct visualization using a flurochrome-conjugated primary antibody. In contrast, detection of a phosphorylation-dependent epitope in a section of frozen tissue may require antigen retrieval and be dependent on amplified chromogenic visualization. This guide provides information on the IHC/ICC variables listed below and is intended to assist in the design, optimization, and troubleshooting of IHC/ICC experiments.
VARIABLES FOR EXPERIMENTAL DESIGN & OPTIMIZATION VARIABLE
FACTORS
Antigen
Species, expression levels, sample types, & subcellular location
Epitope
Dependence on conformation or post-translation modification
Sample Type
Tissue or cells
Sample Preparation
Cells: adhered cell cultures or cell suspensions No primary antibody, isotype control, absorption control, tissue type control
Fixation Method
Perfusion or immersion (with or without freezing)
Fixative*
Formaldehyde, alcohols, or acetone
Blocking Reagent*
Normal serum, bovine serum albumin (BSA), or non-fat milk
Antigen Retrieval*
Protease-induced Epitope Retrieval (PIER) or Heat-induced Epitope Retrieval (HIER)
Detection Method
Direct or indirect (with or without amplification)
Primary Antibody*
Monoclonal or polyclonal
Secondary Antibody*
Species and label
Labeling Method
Fluorescence or chromogenic
Counterstain* Mounting Reagent Visualization & Analysis
Examples of Fluorescence Detection by IHC & ICC. A. Fluorescence detection by IHC was used to visualize V-set and Ig domain-containing protein 1 (VSIG1) in a frozen section of human testis using anti-human VSIG1 affinity-purified polyclonal antibody (Catalog # AF4818). Tissue was stained using NorthernLights-557 anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue). B. Fluorescence detection was used to measure Glial Fibrillary Acidic Protein (GFAP) expression in differentiating cultures of rat cortical stem cells using anti-human GFAP affinity-purified polyclonal antibody (Catalog # AF2594). The cells were stained using anti-sheep NorthernLights-557 secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
A.
Fluorochromes and spectral properties
B.
Chromogens: 3,3' Diaminobenzidine (DAB), 3-amino-9-ethylcarbazole (AEC) Fluorescence: 4,6'-diamidino-2-phenylindole (DAPI) Chromogenic: hematoxylin Fluorescence: anti-fade mounting medium Chromogenic: aqueous mounting medium Fluorescence microscope or standard light microscope
* These variables require optimization of additional factors including concentration, pH, temperature, incubation time, and diluent. This table is not intended to be comprehensive but represents the most commonly applied IHC/ICC methods at R&D Systems.
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B.
Tissue: embedded or frozen
Appropriate Controls
Label
A.
Examples of Chromogenic Detection by IHC & ICC. A. Chromogenic detection of Interferon Regulator Transcription Factor 8 (IRF8) expression in paraffin-embedded human lymphoma tissue section using anti-human IRF8 affinity-purified polyclonal antibody (Catalog # AF5117). The tissue was stained using the anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue). B. Chromogenic detection of IFN-g expression in human peripheral blood mononuclear cells, stimulated with calcium ionomycin (0.5 mg/mL) and PMA (50 ng/mL), using anti-human IFN-g affinity-purified polyclonal antibody (Catalog # AF285). The cells were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
For research use only. Not for use in diagnostic procedures.
INTRODUCTION Sample Preparation Tissue and cell samples must be appropriately harvested and prepared for each IHC/ICC study. To facilitate the required incubation steps, whole tissues must be cut into ultra thin (5-10 mm) sections or cut into smaller pieces for whole mount IHC. For ICC experiments, cells must be attached to a microscope slide or coverslip before commencing the staining procedure. Sample preparation is also intimately linked to the method of fixation, which in turn is influenced by the desired detection technique (fluorescence versus chromogenic). In most circumstances, one experimental variable will determine the most appropriate method of sample preparation. For example, tissue that is immersion-fixed in formaldehyde must be paraffin-embedded and cut using a microtome. Alternatively, to detect a phosphorylationdependent epitope, tissue may need to be snap frozen, which requires a cryostat for sample sectioning followed by alcohol fixation.
Paraffin-embedded Tissue
IHC SAMPLE PREPARATION COMPARISON
Paraffin embedding offers the best option for long-term preservation of tissue samples. Tissue must be fixed before being embedded in paraffin. Fixation can be achieved by perfusion or immersion immediately following dissection, and typically requires 4-24 hours. It is not recommended to fix tissue for more than 24 hours because overfixation can cause masking of the antigen (see page 10). If necessary, tissues can be transferred to alcohol after fixation until there is time for the embedding process. Because paraffin is immiscible with water, tissue must be dehydrated be fore adding molten paraffin wax. Dehydration is achieved by immersion in increasing concentrations of alcohol. This approach allows for a gradual change in hydropho bicity and minimizes cell damage. Following dehydration, the tissue is incubated with xylene to clear any remaining ethanol. Paraffin is typi cally heated to 60 ˚C for embedding and is subsequently allowed to harden overnight. The tissue is subsequently cut with a sharp blade into ultra thin slices using a microtome. Sections are then dried onto microscope slides and can be stored at room temperature for extended periods of time. Tissue sections must be rehydrated before commencing the IHC/ICC protocol (see page 14). Paraffin is the cheapest and most commonly used substance for tissue embedding. However, tissue can also be embedded in plastic, which sets harder and allows thinner tissue sections (1.5 mm versus 5 mm).
Frozen Tissue One of the benefits of frozen tissue samples is the time saved by omitting the initial fixation step required for paraffin-embedded tissue. Snap freezing can be particularly beneficial when detecting post-translational modifications such as phosphorylation. Frozen tissue can be prepared by immersing the tissue in liquid nitrogen or isopentane, or by burying it in dry ice. For frozen samples, a short fixation is done after freezing and sectioning. Frozen tissue sections are most often fixed using alcohol, which avoids the possible requirement to retrieve epitopes masked by formaldehyde cross-linking. Frozen tissue is cut on a cryostat and sections can be stored at - 80 ˚C for up to 1 year.
TISSUE FIXATION
Paraffin-embedded
Frozen
Pre-embedding
Post-sectioning
Microtome
Cryostat
Multiple years at room temperature
1 year at - 80 ˚C
ADVANTAGES
Preserves tissue morphology
Preserves enzyme and antigen function
LIMITATIONS
Overfixation can mask the epitope
Formation of ice crystals may negatively affect tissue structure
SECTIONING STORAGE
Cell Samples To perform ICC studies, the cells must first be attached to a solid support, such as a microscope slide or coverslip. This is simple with adherent cells, which can be grown directly on high-grade, sterilized coverslips placed on the bottom of a 6- or 24-well plate. Cell adhesion can be enhanced by precoating the coverslip with a charged polymer like Poly-L-Lysine and/or an extracellular matrix protein such as Laminin, Fibronectin, or Collagen. All steps of the staining process can be performed with the coverslip in the microplate by adding the appropriate solution to each well and removing solutions by aspiration. Cells in suspension can be adhered to a slide by Cytospin® centrifugation or by a 10 minute incubation of the cells on slides that have been coated with Poly-L-Lysine. Note: It is important to be able to recognize artifacts of poorly prepared samples in order to properly interpret results. For example, folds or tears in the tissue, or bubbles between the tissue/cells and the slide, can collect staining reagents non-specifically and obscure positive staining or lead to false-positive signals.
Frozen tissue sections can be processed in a shorter amount of time than paraffin-embedded sections. However, freezing is not adequate for longterm preservation of the tissues and the formation of ice crystals within the cells may negatively affect subcellular detail. In addition, frozen sections are often thicker than paraffin sections. This can increase the potential for lower microscopic resolution and poor images of tissue morphology. Because frozen tissue often retains enzymatic activities, blocking the functions of endogenous enzymes that may affect the IHC detection method is also particularly important (see page 5).
Cytospin is a registered trademark of Thermo Electron Corporation.
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INTRODUCTION Fixation All samples used in IHC/ICC experiments must be fixed to preserve tissue morphology and retain the antigenicity of the target molecules. Fixation alters the chemical composition of tissues and often requires a compromise between preserving tissue structure and preserving the epitope. Incomplete fixation (underfixation) of cells or tissues may allow rapid proteolytic degradation of target proteins within the tissue and can reduce specific immunoreactivity. However, excessive fixation (overfixation) may result in masking of the epitope or in strong non-specific background staining that can obscure specific labeling. As discussed in the previous section, the method and timing of fixation must be considered in terms of sample preparation. In addition, duration, temperature, and pH will all affect the degree of fixation.
Formaldehyde
Acetone
Formaldehyde is the most common fixative used to preserve protein targets within tissues and cells. Formaldehyde-mediated tissue fixation is thought to be dependent on the formation of protein-protein and proteinnucleic acid cross-links involving methylene bridges (-CH2-). Formaldehyde can chemically link NH2 (amino) and CONH (peptide) groups, NH2 and NH, or NH2 and NH2 groups.
Acetone is a strong dehydrating agent, which can cause irreversible pre cipitation of tissue proteins. It is usually used on sections of unfixed, snapfrozen tissues, followed by fixation with alcohols or formaldehyde.
Formaldehyde is a good choice for most IHC/ICC applications but is not a universal fixative. Overfixation with formaldehyde can modify the amino acids that are part of an epitope and can block an antibody from binding to it. However, in most cases the epitope can be unmasked to restore antibody bind ing using antigen retrieval techniques (see page 10). Formaldehyde has been shown to induce intracellular translocation of phosphorylation-dependent epitopes from the membrane to the cytoplasm. In situations like this, ice cold absolute methanol or absolute ethanol are appropriate alternative fixatives. Note: Although there are many different fixatives available, formaldehyde is the most commonly used, and is an appropriate starting point for most IHC/ICC experiments. Formaldehyde-based solutions should be aliquoted and frozen, or stored at 4-8 ˚C for no longer than one month. See page 12 for a protocol for making a typical 4% formaldehyde solution.
Alcohols The most popular alcohols used for cell and tissue fixation are methanol and ethanol. The molecular structures of methanol and ethanol closely resemble that of water. Therefore, they compete with water for protein hydrogen bonds, replacing water molecules in tissues. This causes pro teins to precipitate at their isoelectric points by reducing their dielectric constant, and can block antibody-epitope binding due to changes in conformation. While alcohols affect the tertiary structure of proteins by breaking hydrophobic bonds, it appears that they can stabilize the secondary structure of proteins. However, it is generally thought that alcohols do not preserve tissue morphology as well as formaldehyde-based fixatives. Alcohols do not penetrate as well as formaldehyde and are primarily used to fix frozen tissue sections and cells. Consequently, alcohol fixation is more suitable for membrane surface antigens. Antigen retrieval is not recommended following alcohol fixation as it is generally considered too harsh and will likely compromise the integrity of the tissue sections or cells.
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Fixation of Tissues Whole animal perfusion fixation is often the best way to preserve antigens when studying intact tissues of small animals such as mice, rats, and guinea pigs. It involves replacing the animal’s systemic blood with a fixative solution. However, whole animal perfusion may not be sufficient to fix the tissues of interest. In cases such as these, dissected tissues can be immersed in the fixative. For example, 4% formaldehyde in PBS is a commonly used solution for perfusion and immersion fixation of tissues. To enhance penetration of the fixative during immersion fixation, it is recommended that tissues be no thicker than 10 mm. For complete fixation, the volume of the fixative should be 50-100 times larger than the volume of the tissue. Fixation is usually achieved at room temperature for 4-24 hours. It is very important to optimize fixation conditions since underfixation or overfixation may reduce or abolish tissue immunoreactivity.
Fixation of Cultured Cells In contrast to tissue samples, cultured cells can be fixed for a shorter period of time with a less concentrated fixative solution. For example, fix ation with a 2% formaldehyde solution for 20 minutes at room tempera ture is often sufficient to preserve both cellular morphology and antigenicity. Fixation of cultured cells is usually achieved by simply discarding the culture medium and adding the fixative solution. However, the change in surface tension following removal of the culture medium can damage some cell types. If this is the case, the fixative can be added directly into the culture medium. For example, adding the same volume of 4% form aldehyde as the volume of culture medium will result in a 2% formalde hyde solution, which is strong enough to pre-fix the cells. After 2 minutes, the pre-fixation culture medium should be replaced with a fresh volume of 2% fixative. The pre-fixation step makes the cells more rigid so they can withstand any potential deleterious effects created by changes in surface tension. Note: Fixation can result in hydrophobic cross-linking of tissue proteins. The time, temperature, pH, and fixative used will determine the degree of cross-linking. Once the fixation protocol has been optimized, the same procedure should be used consistently.
For research use only. Not for use in diagnostic procedures.
INTRODUCTION Preventing Non-specific Staining Once the tissue or cell samples have been appropriately prepared and fixed, the samples are ready to be stained. All IHC/ICC studies are dependent on specific antibody-epitope binding, which is governed by hydrophobic interactions, ionic interactions, hydrogen bonding, and other intermolecular forces. However, the same attractive forces can also result in non-specific staining, i.e. binding of the primary antibody to amino acids other than those within the desired epitope of the antigen. This is a common problem that occurs in IHC/ICC experiments. The challenge is to reduce non-specific interactions without impairing antibody-epitope binding. Causes of non-specific staining include interactions of the primary and secondary antibodies with serum proteins, ionic interactions between antibodies and tissues, and interactions with endogenous molecules capable of affecting the IHC detection system used. These issues can result in high background and an inability to visualize the antigen of interest in its appropriate cellular location. Staining problems of this type can be addressed by blocking non-specific interactions using a blocking reagent. These steps are performed prior to incubation of the sample with the primary antibody.
Preventing Non-specific Hydrophobic Interactions Although hydrophobic interactions play an essential role in epitopeantibody binding, these forces can also promote non-specific binding. Most proteins have some degree of hydrophobicity due to the neutral side chains of several amino acids. Tissue incubation with heat-inactivated normal serum or bovine serum albumin (BSA) is a common procedure used to reduce non-specific hydrophobic binding. Selection of the type of normal serum is important to prevent interactions with the primary or secondary antibodies, or with the tissues/cells being stained. For example, goat serum would not be advised as a blocking reagent for use with a goat-derived primary antibody. Instead, a serum identical to the host animal of the secondary antibody or from an unrelated species is recommended. BSA and non-fat dry milk are also frequently used as blocking reagents. One of these reagents is typically included in the diluents for the primary and secondary antibodies. The addition of non-ionic detergents including 0.3% Triton X-100™ or Tween 20™ can also reduce nonspecific hydrophobic interactions.
Chromogenic detection methods often use a conjugated enzyme to vis ualize epitope-antibody interactions. When using this method of detection, the endogenous activity of the same enzyme must be blocked. For example, protocols that include horseradish peroxidase (HRP) or alkaline phospha tase (AP) may require reagents to prevent non-specific signals. Tissues such as kidney, liver, or vascular areas with red blood cells, contain endogenous peroxidase activity. Peroxidase blocking reagents formulated with 3-10% H2O2 can be used to prevent endogenous peroxidase from cleaving the substrate. Endogenous AP found in intestine, kidney, lymphoid and other tissue can be blocked with 1 mM Levamisole. The intestinal form of AP is unaffected by Levamisole but can be blocked by using 1% acetic acid.
A.
A.
B.
Blocking Non-specific Binding with Serum. A. CD14 was detected in paraffin-embedded human tonsil tissue using anti-human CD14 biotinylated affinity-purified polyclonal antibody (Catalog # BAF383). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using high sensitivity streptavidin conjugated to HRP (HSS-HRP) and DAB, and counterstained with hematoxylin (blue). B. Non-specific background staining is markedly reduced in a parallel experiment which included a blocking step using animal serum for 15 minutes at room temperature prior to incubation with the primary antibody. HSS-HRP and animal blocking serums are included in all R&D Systems Cell and Tissue Staining Kits (see page 112 for available kits).
Preventing Non-specific Ionic Interactions If the antibody employed and the target tissue have net opposite charge, ionic interactions can cause non-specific background staining. For ex ample, non-specific staining may be due to attraction between opposing carboxyl and amino groups. Van der Waals forces, weak electrostatic interactions between dipolar molecules, can also contribute. Increasing the ionic strength of the fixative and/or the antibody diluent buffer can reduce ionic interactions. However, epitope-antibody binding is common ly dependent on ionic forces, so this approach also has the potential to
negatively impact staining specificity. Due to their single epitope speci ficity, increasing ionic strength is more likely to impair monoclonal than polyclonal antibody performance.
Endogenous Enzyme Interference
B.
Quenching Endogenous Peroxidase Activity. A. Failing to quench endogenous peroxidase prior to staining produced a false positive signal in sections of human kidney. Tissue was stained using the anti-goat HRP-DAB Cell & Tissue Staining Kit (Catalog # CTS008; brown). B. Endogenous peroxidase activity was quenched in the same tissue using 3% H2O2 in methanol for 15 minutes at room temperature prior to staining.
Endogenous Biotin Interference Streptavidin binding to a biotinylated primary or secondary antibody is another common detection system that is utilized for IHC/ICC experiments (see page 7). Therefore endogenous biotin must be blocked before strepta vidin incubation. Endogenous biotin is found in many tissues including liver, kidney, heart, brain, and lung. Pre-incubation of the sample with avidin is routinely used to block endogenous biotin. This must be followed by a sub sequent incubation with biotin to block additional biotin binding sites on the avidin molecule. Following these biotin/avidin blocking steps, the sample can be incubated with the primary antibody.
Triton X-100 is a trademark of Dow Chemical Corporation. Tween is a trademark of ICI Americas.
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INTRODUCTION Primary Antibody Selection & Optimization The most important factor when designing an IHC/ICC experiment is selection of the primary antibody. In turn, the critical feature of a primary antibody is specificity for the epitope. All steps of an IHC/ICC experiment must be optimized to visualize specific staining and minimize non-specific background signals. This includes performing initial studies to determine the appropriate incubation conditions for each primary antibody. The working dilution for an antigen affinity-purified polyclonal antibody is generally lower than that of a monoclonal antibody but these values must be determined empirically. To achieve a robust and specific signal, a high quality antibody that exhibits minimal cross-reactivity should be employed.
Selecting a Primary Antibody Supplier When searching for a primary antibody, it is likely that products will be available from several different commercial sources that may offer varying degrees of antibody quality. This initial choice can make the difference between a successful experiment and a missed opportunity. A first step is to look for independent verification. This includes checking for prior successful use of the antibody in the literature. Many commercial suppliers, including R&D Systems, can provide assistance to researchers looking for this information. Another potential question to ask is whether the antibody is actually manufactured by the supplier or is it purchased and resold? Purchasing direct ensures the commercial source has had complete control of manufacturing, quality control processes, and shipping and storage conditions. In addition, the manufacturer may be in the best position to provide quality technical service should questions arise about the use of the antibody.
Selecting a Monoclonal or Polyclonal Antibody The inherent features of monoclonal and polyclonal antibodies are as sociated with advantages and limitations when applied to IHC/ICC. Mono clonal antibodies, produced from a single B cell clone, represent a homo geneous population that bind with high affinity and specificity to a single epitope. This can be particularly useful when detecting a single member of a protein family that shares a high percentage of amino acid identity. Antibody binding is often dependent on the target protein maintaining its native conformational state. Access to a desired epitope can be com promised by interactions with other proteins, post-translational modifica tions, temperature, pH, fixation, and salt concentration. Because polyclonal antibodies are heterogeneous and can recognize multiple epitopes, they are less likely to be affected by changes in protein conformation. In general, polyclonal antibodies are also more stable than monoclonal antibodies over a range of pH and salt concentration. For these reasons, polyclonal anti bodies are more frequently used for IHC/ICC experiments than monoclonal antibodies.
Affinity Purification of Polyclonal Antibodies Polyclonal antiserum contains a mixture of antibodies produced by a large number of B cell clones. Antibodies comprising polyclonal antiserum bind to target epitopes with different specificities and affinities, as well as crossreact with irrelevant target molecules (non-specific interactions). To enrich for antibodies with the highest specificity and affinity toward the antigen of interest, R&D Systems polyclonal antibodies are antigen affinity purified. During this process polyclonal antiserum is passed through an affinity column containing immobilized antigen molecules. Specific antibodies are retained by the immobilized antigen, whereas non-specific antibodies pass through the column and are discarded. The retained antigen affinity purified antibodies are subsequently eluted from the column. Antigen af finity purified antibodies interact predominantly with their target antigen,
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reduce background staining, and produce more consistent results com pared to non-purified antibodies. Monoclonal Antibody Binding
Polyclonal Antibody Binding
Monoclonal and Polyclonal Antibody Binding. Monoclonal antibodies bind only a single epitope, while polyclonal antibodies bind different epitopes on the same protein,
Optimization of Primary Antibody Incubation Primary antibody concentration, diluent, incubation time, and temperature all impact the quality of staining. These variables need to be optimized for each antibody and sample to achieve specific staining and low background. Often optimization is approached by maintaining a constant incubation time and temperature, while varying the antibody concentration to deter mine when an optimal signal is achieved with low background noise. For example, if a high affinity antibody is used, then the antibody can be used at a relatively high concentration for a shorter incubation time. Alternatively, a high affinity antibody at lower antibody concentration may be used with a longer incubation time. Longer incubation durations are often employed to ensure penetration throughout tissue sections used for stereological techniques. To promote specific staining, longer incubation periods are often conducted at lower temperatures (i.e. 4 ˚C versus room temperature). Optimization of R&D Systems staining protocols for tissue sections typi cally begin with an overnight incubation with the primary antibody at 4 ˚C. For staining cells, a 1 hour incubation with the primary antibody at room temperature is a common starting point. The working dilution of an anti gen affinity purified polyclonal antibody is generally lower (from 1.7-15 mg/mL) than that of a monoclonal antibody (5-25 mg/mL). When comparing samples stained with different concentrations of the same antibody, it is important to use a consistent time and temperature for the incubation step. When working with a new antibody for the first time, it is recom mended to conduct preliminary studies testing a broad range of antibody concentrations.
STARTING CONDITIONS FOR PRIMARY ANTIBODY INCUBATION ANTIBODY
SAMPLE
Monoclonal
Polyclonal
Tissue
5-25 mg/mL, overnight at 4 ˚C
1.7-15 mg/mL, overnight at 4 ˚C
Cells
5-25 mg/mL, 1 hour at room temperature
1.7-15 mg/mL, 1 hour at room temperature
Advantage
Single epitope specificity
Lower concentration required
Limitation
Vulnerable to epitope masking
Heterogeneous population
For research use only. Not for use in diagnostic procedures.
INTRODUCTION Detection & Visualization Following incubation with the primary antibody, antibody binding is visualized using an appropriate detection system. The method of detection can be direct or indirect, and may generate a fluorescent or chromogenic signal. Direct detection involves the use of primary antibodies that are directly conjugated to a label. Indirect detection methods utilize a labeled secondary antibody raised against the primary antibody host species. Indirect methods can also include amplification steps to increase signal intensity. Commonly used labels for the visualization of epitope-antibody interactions include fluorophores and enzymes that convert soluble substrates into insoluble, chromogenic end products. The choice of label is affected by the detection method employed, personal preference, and the type microscope equipment that is available
Direct or Indirect Detection, & Amplification
DETECTION METHODS & SIGNAL INTENSITY
The choice of whether to use direct or indirect detection is often dictated by the level of antigen expression. For example, detection of a highly expressed epitope might be possible using a primary antibody directly conjugated to a label. Advantages of direct detection include the ease of use for multicolor staining and the elimination of concerns regarding nonspecific binding of the secondary antibody. The major disadvantage is that direct detection may lack the sensitivity required to visualize lower expression levels. In rare cases the conjugation process may also have a negative impact on the affinity of the primary antibody.
DIRECT
ADVANTAGE
LIMITATION
Good for multicolor labeling
Lower sensitivity
In contrast, indirect detection methods generally have a higher level of sensitivity and generate a more intense signal. The signal is amplified using the indirect method because of the potential for at least two labeled secondary antibodies to bind to each primary antibody. However, the use of a secondary antibody requires extra blocking steps and additional controls. Further amplification of the signal can be achieved by taking advantage of the strong affinity of avidin and streptavidin to bind biotin. Avidin is a glycoprotein in egg white that combines stoichiometrically with biotin. Streptavidin is purified from the bacterium Streptomyces avidinii, is not glycosylated, and exhibits lower non-specific binding than avidin. Both proteins bind four biotins per molecule. If a biotinylated secondary anti body is employed, the signal can be significantly amplified by subsequent incubation with an avidin-biotin complex (ABC Method), or labeled streptavidin-biotin (LSAB Method). Streptavidin may be conjugated to a detection enzyme (i.e. horseradish peroxidase or alkaline phosphatase) or fluorochrome. Using these amplification methods requires additional steps to prevent non-specific binding (see page 5).
KEY:
INDIRECT
ADVANTAGE
LIMITATION
Higher sensitivity
Potential non-specific binding of secondary antibodies AMPLIFICATION – ABC METHOD
ADVANTAGE
LIMITATION
Increased signal intensity
Requires additional time & controls
Antigen AMPLIFICATION – LSAB METHOD
Primary Antibody Secondary Antibody Label Avidin/Streptavidin Biotin
ADVANTAGE
LIMITATION
Increased signal intensity
Requires additional time & controls
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INTRODUCTION Fluorescence Detection
Multicolor experiments must be designed to limit cross-reactivity between the detection reagents and crossover between the spectral properties of the fluorochromes being used. To limit cross-reactivity between secondary reagents, the primary antibodies should be derived from different species. This allows for the use of species-specific secondary antibodies that recognize only one primary antibody. However, there may be some instances when it is necessary to simultaneously use antibodies from the same species. This can be achieved using a biotinylated form of one of the primary antibodies. When using this technique, tissues must be incubated with the non-biotinylated antibody first, followed by incubation with the fluorophore-conjugated secondary antibody. The tissues are then incubated with the biotinylated primary antibody followed by incubation with a streptavidin-conjugated fluorochrome. This approach will ensure that the streptavidin conjugate will only bind the biotinylated antibody, limiting cross-reactivity.
Emission Filter Dichroic Mirror TYPICAL FLUORESCENCE FILTER SET Excitation Filter Sample
Multicolor Fluorescence Microscopy. A typical immunofluorescence setup that depicts the light source, a suitable filter set for the fluorochrome of interest, and a detection mechanism.
NorthernLights is a trademark of R&D Systems.
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400
450
500
550
600
650
700
Wavelength (nm)
B.
R&D Systems NorthernLights™ Fluorochromes have Non-overlapping Spectral Proper ties that make them Ideal for Multicolor IHC and ICC. A. Emission curves for NorthernLights-493 anti-goat IgG (Catalog # NL003; green) and NorthernLights-557 anti-mouse IgG (Catalog # NL007; red). B. Multicolor detection of Nestin and b-III Tubulin in rat cortical stem cells (Catalog # NSC001) using anti-rat Nestin affinity-purified polyclonal antibody (Catalog # AF2736; green) and anti-neuron specific b-III Tubulin monoclonal (clone TuJ-1) antibody (Catalog # MAB1195; red). Cells were stained with NorthernLights-493 (Catalog # NL003; green) and NorthernLights-557 (Catalog # NL007; red) secondary antibodies and counterstained with DAPI (blue).
Detection
Excitation Light Source
A.
Emission Intensity
Fluorescence detection is based on the use of fluorochromes that emit light when excited by light of a shorter wavelength. The fluorochrome can be conjugated directly to the primary or secondary antibody, or to streptavidin. Immunofluorescence is commonly used for the simultaneous visualization of multiple cellular targets. For example, tissues can be incubated with a mixture of different primary antibodies followed by incubation with secondary reagents conjugated to fluorochromes that emit light at different wavelengths.
Chromogenic Detection To facilitate chromogenic detection, the primary antibody, secondary anti body, or streptavidin is conjugated to an enzyme. When a soluble organic substrate is applied, the enzyme reacts with the substrate to generate an insoluble colored product that is localized to the sites of antigen expression. Commonly used enzymes include horseradish peroxidase (HRP) and alkaline phosphatase, which convert 3,3' diaminobenzidine (DAB) and 3-amino-9-ethylcarbazole (AEC), into brown and red end products, re spectively. DAB is more commonly used than AEC since the latter is soluble in alcohol and is more prone to fade when exposed to excessive light. Aqueous counterstain and mounting media must be used with AEC. Chromogenic detection is considered to be more sensitive than that of immunofluorescence, but is less convenient because it includes more incubation and blocking steps. Like immunofluorescence, chromogenic detection allows for the visualization of multiple antigens, but only if the antigens are confined to different locations in the cell and tissue because overlapping colors may obscure results. An advantage of DAB chromogen ic staining is that the colored precipitate formed during the reaction between HRP and DAB is not sensitive to light and the slides can be stored for many years. Unlike fluorescence microscopy, which requires a specialized light source and filter sets, chromogenic techniques require only a typical light microscope.
For research use only. Not for use in diagnostic procedures.
INTRODUCTION IHC/ICC Controls Appropriate controls are critical for the accurate interpretation of IHC/ICC results. A satisfactory IHC/ICC experimental design produces results that demonstrate that the antigen is localized to the correct specialized tissues, cell types, or subcellular location. Optimization of fixation, blocking, antibody incubation, and antigen retrieval steps will generate a strong and specific signal. However, IHC/ICC experiments must include positive and negative controls to support the validity of staining and identify experimental artifacts. In addition, variances in antibody specificity, experimental conditions, biological conditions between tissue types, and even researchers may generate inconsistent staining and lead to inaccurate conclusions. To facilitate consistent performance, detailed record keeping is an important factor for IHC/ICC studies. Here, we describe several established controls that can be used to support the specificity of your IHC/ICC results.
Endogenous Tissue Background Control Certain cells and tissues may have inherent biological properties resulting in background staining that could lead to a misinterpretation of the re sults. Before applying primary antibodies, cells and tissues should be in spected under the microscope using either fluorescence (for fluorescent labels) or bright-field (for chromogenic labels) illumination to ensure there is no signal inherent to the tissue itself. For instance, lipofuscin is an endogenous autofluorescent pigment that can be confused with positive staining.
A.
B.
C.
The antigen to antibody mixture should be made at a working dilution of 10:1 (molar ratio) and be pre-incubated overnight at 4 ˚C. The pre-absorbed antibody can then be incubated with tissue in place of the primary antibody alone. The staining pattern produced by the primary antibody can be compared to that produced by the pre-absorbed antibody. Absorption controls work better if the immunogens are peptides. However, if antibodies were raised against the whole protein, addition of the mixture of antibody plus protein may result in higher non-specific staining. Although the mechanism is unclear, the antigen used for pre-absorption may itself bind to the tissue and result in non-specific staining. Thus, it is important to note that an absorption control using whole protein may not always confirm the specificity of an antibody for the protein in the tissue.
A.
B.
Lipofuscin Background in Nervous System Tissue. Lipofuscin is a pigment that accumulates with age in many tissue types. It also has autofluorescent properties that overlap with the excitation and emission spectra of commonly used fluorochromes. Circled in the micrographs above are lipofuscin-containing neurons that may appear labeled using either bright-field microscopy (A) or fluorescence microscopy in the green (B) and red spectrums (C).
No Primary Antibody Control A control in which the tissue is incubated with antibody diluent, without the primary antibody included, is always necessary. This is followed by incubation with secondary antibodies and detection reagents. Staining with detection reagents alone should be negligible to the point that it does not obscure specific staining or resemble the specific staining pattern.
Isotype Control This control can be utilized when working with monoclonal primary antibodies. The sample is incubated with antibody diluent, supplemented with a non-immune immunoglobulin of the same isotype (for example, IgG1, IgG2A, IgG2B, IgM) and concentration as the primary monoclonal anti body. The sample is then incubated with the secondary antibody and de tection reagents. These steps will help ensure that what appears to be specific staining was not caused by non-specific interactions of immuno globulin molecules with the sample. Background staining should be neg ligible and not resemble specific staining.
Absorption Control To demonstrate that an antibody is binding specifically to the antigen of interest, it is first pre-incubated with the immunogen. This should inactivate the antibody and the tissue should show little or no staining.
Absorption Control in Rat Dorsal Root Ganglion. A. A cryostat section of rat dorsal root ganglion stained for phospho-MSK1 (S212) using anti-human Phospho-MSK1 (S212) affinity purified polyclonal antibody (Catalog # AF1036). B. Nuclear staining (indicated by arrows) is abolished if the antibody is first pre-absorbed with the S212 phosphorylated immunogen.
Tissue Type Control Additional controls for IHC/ICC experiments include using tissue samples that are known to express (or not express) the epitope of interest. This strategy can provide a useful reference and may also be utilized during initial optimization studies. Tissue from transgenic animals that overexpress or do not express the antigen can be particularly useful. In addition, tissue samples from different species can be included to support the speciesspecificity of an antibody.
Limitations of Western Blot Comparisons Western blot experiments are often conducted to complement and support IHC/ICC studies. However, changes in protein conformation during denaturation can result in potentially misleading results. For a given antibody, inconsistent results between Western blot and IHC/ICC studies may simply reflect differences in the experimental conditions employed. Since multiple epitopes are recognized, polyclonal antibodies are less vulnerable to such experimental artifacts.
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INTRODUCTION Antigen Retrieval Although fixation is essential for the preservation of tissue morphology, this process can also have a negative impact on IHC/ICC detection. Fixation can alter protein biochemistry such that the epitope of interest is masked and can no longer bind to the primary antibody. Masking of the epitope can be caused by cross-linking of amino acids within the epitope, cross-linking unrelated peptides at or near an epitope, altering the conformation of an epitope, or altering the electrostatic charge of the antigen. Antigen retrieval refers to any technique in which the masking of an epitope is reversed and epitope-antibody binding is restored.
Antigen Retrieval Techniques The need for antigen retrieval depends on multiple variables, including but not limited to, the target antigen, the antibody used, the type of tissue, and the method and duration of fixation. Because they recognize multiple epitopes, polyclonal antibodies are more likely than monoclonal antibod ies to detect a given antigen without the use of antigen retrieval techniques. There are multiple techniques to restore the immunoreactivity of an epitope. Methods as simple as changing the pH or the cation concentration of the antibody diluent can influence the affinity of an antibody for its epitope. For partially masked epitopes it may be appropriate to first try increasing the primary antibody incubation conditions before commen cing antigen retrieval. However, this step will also require further op timization in terms of appropriate antibody concentration, incubation time, and temperature. When discussing antigen retrieval methods, tech niques generally fall into two main categories, protease-induced epitope retrieval (PIER) and heat-induced epitope retrieval (HIER). Once optimized, the effects of antigen retrieval can be pronounced.
Protease-induced Epitope Retrieval (PIER) In the PIER method, enzymes including Proteinase K, Trypsin, and Pepsin have been used successfully to restore the binding of an antibody to its epitope. The mechanism of action is thought to be the cleavage of pep tides that may be masking the epitope. The disadvantages of PIER are the low success rate for restoring immunoreactivity and the potential for destroying both tissue morphology and the antigen of interest.
Heat-induced Epitope Retrieval (HIER)
artifactual staining should always be considered when using any antigen retrieval methodology. The use of controls to demonstrate specific anti body binding should be included since staining is influenced by multiple variables in any given experiment. TIME
ANTIGEN RETRIEVAL SOLUTION pH Acidic
Neutral
Basic
1 minute
Slide #1
Slide #2
Slide #3
5 minutes
Slide #4
Slide #5
Slide #6
10 minutes
Slide #7
Slide #8
Slide #9
Optimization of HIER. A typical experimental set-up to determine optimum HIER incubation time and pH. Results should be compared to a tenth slide, with no HIER. A similar approach can be employed to optimize incubation temperature.
R&D Systems offers reagents to improve tissue antigen detection and enhance immunoreactivity. During initial optimization, investigators can compare samples treated with neutral pH 7.0 Universal Antigen Retrieval Solution (Catalog # CTS015) with a slide of the same tissue without antigen retrieval. Subsequent tests using acidic or basic antigen retrieval solutions may be necessary depending on the tissue. At R&D Systems, we find that treatment with Basic Antigen Retrieval reagent (Catalog # CTS013) is frequently successful. We also offer Acidic (Catalog # CTS014) and Sampler (Catalog # CTS016) Antigen Retrieval Reagents. Acidic and basic antigen retrieval solutions are more likely to affect tissue morphology than a neutral solution.
No HIER
Acidic HIER
Neutral HIER
Basic HIER
HIER is believed to reverse some cross-links and allows for restoration of secondary or tertiary structure of the epitope. The protocol must be opti mized for each tissue, fixation method, and antigen to be studied. In gen eral, HIER has a much higher success rate than PIER. HIER is performed using microwave ovens, pressure cookers, vegetable steamers, autoclaves, or water baths. Microwaves are an increasingly pop ular appliance for HIER. These protocols tend to involve 5 minute periods of heat followed by replacement of the buffer. For all HIER methods, slides must be cooled before commencing IHC/ICC incubations. HIER is especially time-, temperature-, buffer-, and pH-sensitive, and the best method must be determined empirically.
Optimization & Limitations Antigen retrieval may require increased adherence of the sample to the slide or coverslip. In addition, the technique is often too harsh for cryostat tissue sections and alcohol-fixed tissue. The time, temperature, and pH must also be optimized for each appliance (i.e. 5-10 minutes at 92-95 °C in a water bath, versus 1-5 minutes at 120 °C in a pressure cooker). To optimize antigen retrieval preliminary studies must be conducted using a matrix of time, temperature, and pH combinations. The possibility of
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Antigen Retrieval Improves Detection of p27. IHC images show the detection of p27 in paraffinembedded human prostate cancer sections following incubation of tissue for 10 minutes at 95 ˚C in the specified antigen retrieval solution. Compared to no HIER treatment, p27 detection was enhanced following incubation in neutral (pH 7.0) and basic (pH 9.5) (Catalog # CTS013) but not acidic (pH 5.0) (Catalog # CTS014) antigen retrieval solution. p27 was detected using antihuman/mouse/rat p27 monoclonal antibody (Catalog # MAB22561; brown).
For research use only. Not for use in diagnostic procedures.
IHC/ICC PROTOCOLS
IHC/ICC PROTOCOLS Immunohistochemistry
Immunocytochemistry
BLOCK PRIMARY ANTIBODIES SECONDARY ANTIBODIES LABEL COUNTERSTAIN COVERSLIP
RAGE in Alzheimer’s Brain.
Meteorin in Rat Cortical Stem Cells.
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IHC/ICC PROTOCOLS A. Making a 4% Formaldehyde Solution in PBS The vast majority of IHC/ICC procedures employ fixation of tissues and cells using formaldehyde-based fixatives. The protocol below describes the technique for generating a 4% formaldehyde solution in PBS. The most effective fixative must be determined experimentally. Caution: Formaldehyde is toxic, read the MSDS before working with this chemical. Gloves and safety glasses should be worn, and solutions made inside a fume hood. Please read the protocol in its entirety before starting.
SUPPLIES REQUIRED REAGENTS Deionized H2O HCl (Dilute) NaOH (1 N) Paraformaldehyde powder PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4 , pH 7.4
40 g Paraformaldehyde Powder 1 N NaOH UNTIL POWDER DISSOLVES
MATERIALS Filter units Glassware and stir bar (Dedicated for formaldehyde solution) Gloves and eye protection Hot plate with magnetic stirrer Thermometer Ventilated hood
800 mL 1x PBS AT ≈ 60 ˚C
HEAT WHILE STIRRING
PROCEDURE 1. For 1 L of 4% Formaldehyde add 800 mL of 1x PBS to a glass beaker on a stir plate in a ventilated hood. Heat while stirring to approximately 60 ˚C. Take care that the solution does not boil. 2. Add 40 g of paraformaldehyde powder to the heated PBS solution.
COOL & FILTER WITH FILTER PAPER
3. The powder will not immediately dissolve into solution. Slowly raise the pH by adding 1 N NaOH dropwise from a pipet until the solution clears.
ADJUST THE VOLUME TO 1 L WITH 1x PBS
4. Once the paraformaldehyde is dissolved the solution should be cooled and filtered. 5. Adjust the volume of the solution to 1 L with 1x PBS. 6. Recheck the pH and adjust it with small amounts of dilute HCl to approximately 6.9. 7. The solution can be aliquoted and frozen, or stored at 4 ˚C for no longer than one month.
H C
O
H Formaldehyde
H2O
H
OH C
H
OH Methylene Glycol
Formaldehyde Structure. In an aqueous solution, formaldehyde exists primarily as methylene glycol.
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ADJUST THE pH TO 6.9 WITH DILUTE HCl ALIQUOT & FREEZE
The difference between paraformaldehyde, formaldehyde, & formalin. Paraformaldehyde (chemical name is polyoxymethylene) is a powder of polymerized formaldehyde that by itself cannot fix tissues. To be usable as a tissue fixative, paraformaldehyde has to be dissolved in hot water to become a formaldehyde solution. Formalin is a saturated formaldehyde solution in water (37% by weight, 40% by volume) containing 10-15% methanol. Methanol is added to slow down the polymerization of formaldehyde, which reduces the fixing power of formalin. Formalin can also be made in an alcohol-free form from powdered paraformaldehyde.
For research use only. Not for use in diagnostic procedures.
IHC/ICC PROTOCOLS B. Preparation of Gelatin-coated Slides for Histological Tissue Sections In order for tissue sections to be retained on histological slides during staining and washing steps, slides need to be coated with adhesive compounds. Although there are a variety of such compounds, gelatin is the most frequently used for histological purposes. Please read the protocol in its entirety before starting.
SUPPLIES REQUIRED
5 g Gelatin AFTER THE GELATIN DISSOLVES ADD 0.5 g CrK(SO4)2
REAGENTS Gelatin-coating solution: 1 L deionized H2O, 5 g gelatin, 0.5 g chromium potassium sulfate CrK(SO4)2 MATERIALS Filter units Histological slides Hot plate with magnetic stirrer Slide racks Staining dish Thermometer
HEAT AT 45 ˚C WHILE STIRRING
1 L Deionized H20
PROCEDURE 1. Prepare the gelatin-coating solution by dissolving 5 g of gelatin in 1 L of heated, deionized H2O (temperature should not exceed 45 ˚C). 2. After the gelatin has dissolved, add 0.5 g of chromium potassium sulfate. Chromium potassium sulfate will positively charge the slides allowing them to attract negatively charged tissue sections. 3. Filter this solution and store at 2-8 ˚C until use. It is recommended that this solution be filtered again immediately before use (adjust to room temperature before filtration).
COOL & FILTER AT ROOM TEMPERATURE
ADD Gelatin-coating Solution
4. Place the histological slides into metal racks.
DIP RACKS WITH SLIDES 3 to 5 TIMES, 5 SECONDS EACH
Note: The slides should be cleaned by washing them in soapy water and rinsing them thoroughly, first in tap water and finally in deionized water. 5. Dip the racks containing the slides 3 to 5 times (~5 seconds each) into the gelatin-coating solution. 6. Remove the racks containing the slides and let them drain. Blot excess solution from the racks onto filter paper (gently tap the racks against the filter paper for better drainage).
Staining Dish
Histological Slides
7. Place the racks containing the slides on the lab bench and cover them with paper towels to protect them from dust. 8. Dry at room temperature for 48 hours. 9. Dried slides can be put back into the boxes that they arrived in and stored at room temperature until use. Slides intended for cryostat sections can be stored at -20 ˚C.
DRY AT ROOM TEMPERATURE FOR 48 HOURS
STORE AT ROOM TEMPERATURE OR AT -20 ˚C IF INTENDED FOR CRYOSTAT SECTIONS
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IHC/ICC PROTOCOLS C. Chromogenic Staining of Tissue Sections Using HRP-DAB Detection The following protocol has been developed and optimized by R&D Systems IHC/ICC laboratory using the HRP-DAB Cell and Tissue Staining Kits (see page 112). Protocols may need to be modified depending on the type of tissue used. Each investigator should determine the optimal working dilution of an antibody. If using R&D Systems primary antibodies, refer to the product data sheets to obtain approximate working dilutions. For all other reagents follow the manufacturer’s instructions. Caution: DAB is hazardous. Gloves and safety glasses should be worn, and all steps performed inside a fume hood. Please read the protocol in its entirety before starting.
SUPPLIES REQUIRED REAGENTS Aqueous Mounting Medium (Catalog # CTS011, or equivalent) Primary Antibodies Cell and Tissue Staining Kit: (See page 112) Anti-goat (Catalog # CTS008), Anti-mouse (Catalog # CTS002), Anti-rabbit (Catalog # CTS005), Anti-rat (Catalog # CTS017) Kit contents: Peroxidase Blocking Reagent, Avidin Blocking Reagent, Biotin Blocking Reagent, Biotinylated Secondary Antibodies, High Sensitivity Streptavidin-HRP Conjugate (HSS-HRP), DAB Chromogen Solution, Serum Blocking Reagents Deionized H20 Dilution buffer: PBS (1x), 1% bovine serum albumin, 1% normal donkey serum, 0.3% Triton™ X-100, and 0.01% sodium azide PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4 , pH 7.4 Wash buffer: PBS (1x) For Paraffin-embedded tissue sections : Xylenes (mixed isomers) 100% denatured alcohol 95% denatured alcohol 70% denatured alcohol 50% denatured alcohol OPTIONAL REAGENTS Antigen retrieval reagents (see page 17) DAB Enhancer (Catalog # CTS010)
If necessary, perform antigen retrieval (see page 17 for protocol). If endogenous peroxidase is present in the tissue 3. Incubate the sample with 1-3 drops peroxidase blocking reagent for 5 minutes Note: This procedure may affect the morphology of unfixed frozen tissue sections. Reduction in the incubation time may be required. 4. Rinse the sample, then gently wash in wash buffer for 5 minutes. 5. Surround the tissue with a hydrophobic barrier using a barrier pen. Proceed to the staining protocol on page 15.
CRYOSTAT SECTIONS THAW SLIDES AT ROOM TEMPERATURE 10-20 MINUTES
MATERIALS Coverslips Hydrophobic barrier pen Slides with paraffin-embedded or cryostat tissue sections
PROCEDURE
PARAFFIN-EMBEDDED TISSUE SECTIONS 95% Alcohol
70% Alcohol
50% Alcohol
2 times 2 times 1 time 10 minutes 10 minutes 5 minutes
1 time 5 minutes
1 time 5 minutes
Xylene
100% Alcohol
REHYDRATE THE SLIDES WITH Wash Buffer FOR 5 MINUTES
1. Slide preparation for Cryostat Sections
RINSE WITH Deionized H20
a. When staining cryostat sections stored in a freezer, thaw the slides at room temperature for 10-20 minutes. Slide preparation for Paraffin-embedded Tissue Sections a. Immerse the slides in xylene (mixed isomers) 2 times for 10 minutes each. b. Immerse the slides in 100% alcohol 2 times for 10 minutes each. c. Immerse the slides in 95% alcohol for 5 minutes. d. Immerse the slides in 70% alcohol for 5 minutes. e. Immerse the slides in 50% alcohol for 5 minutes. f. Rinse the slides with deionized H20. 2. Rehydrate the slides with wash buffer for 10 minutes. Drain the excess wash buffer. If proceeding directly to one of the staining protocols on pages 15 or 16, surround the tissue with a hydrophobic barrier using a barrier pen.
14
For research use only. Not for use in diagnostic procedures.
DRAIN EXCESS Wash Buffer AND PERFORM ANTIGEN RETRIEVAL IF NECESSARY
IHC/ICC PROTOCOLS Chromogenic Staining Using HRP-DAB Detection
PROCEDURE 1. Block the slides with 1-3 drops of serum blocking reagent for 15 minutes. Drain the slides and wipe away any excess blocking reagent before proceeding to the next step. Do not rinse.
BLOCKING (FOLLOW STEPS 1-3)
2. Incubate the sample with 1-3 drops of avidin blocking reagent for 15 minutes. Rinse the sample with wash buffer, drain slides, and wipe away any excess wash buffer. 3. Incubate the sample with 1-3 drops of biotin blocking reagent for 15 minutes. Rinse with wash buffer, drain the slides, and wipe away any excess wash buffer. 4. Incubate the sample with primary antibodies in dilution buffer. Follow manufacturer’s recommendations regarding working dilution, incubation time, and temperature. These variables may need to be modified for your system.
RINSE WITH Wash Buffer, DRAIN SLIDES ADD Primary Antibodies INCUBATE THE SAMPLE ACCORDING TO THE MANUFACTURER’S RECOMMENDATIONS
5. Rinse the sample with wash buffer and then wash 3 times in wash buffer for 5 minutes each and drain the slides. 6. Incubate the sample with 1-3 drops of biotinylated secondary antibodies for 30-60 minutes, adjusting the incubation time depending on the thickness of the section (approximately 30 minutes for 5-10 μm thick sections and 60 minutes for 10-20 μm thick sections).
RINSE 3 TIMES FOR 5 MINUTES EACH WITH Wash Buffer, AND DRAIN ADD Biotinylated Secondary Antibodies
INCUBATE FOR 30-60 MINUTES
7. Rinse the sample with wash buffer then wash 3 times in wash buffer for 15 minutes each and drain the slides.
RINSE 3 TIMES FOR 15 MINUTES EACH WITH Wash Buffer, AND DRAIN
8. Incubate the sample with 1-3 drops of HSS-HRP for 30 minutes. 9. Rinse the sample with wash buffer then wash 3 times in wash buffer for 2 minutes each. 10. Calculate the working volume of DAB Chromogen Solution given that 100-200 μL is required to cover the entire tissue section on a slide. Add 1-5 drops of DAB Chromogen Solution to cover the entire tissue section and incubate for 3-20 minutes. Monitor the intensity of the tissue staining under a microscope. Note: DAB Enhancer can be used to intensify the DAB Chromogen solution. DAB is hazardous. Refer to the MSDS for safe deactivation. 11. Rinse the sample with wash buffer 3 times for 10 minutes each.
ADD HSS-HRP
INCUBATE FOR 30 MINUTES
RINSE AND WASH 3 TIMES IN Wash Buffer FOR 2 MINUTES EACH ADD DAB Chromogen Solution
INCUBATE 3-20 MINUTES MONITORING INTENSITY OF TISSUE STAINING UNDER A MICROSCOPE
12. Rinse in deionized H20 and drain the slides. 13. Stained tissue can be mounted either without counterstaining or counterstained with hematoxylin for better visualization of the tissue morphology. 14. Cover stained tissue with a coverslip of an appropriate size. Place slides vertically on a filter paper or towel to drain excess mounting medium and allow them to dry.
RINSE 3 TIMES FOR 10 MINUTES EACH WITH Wash Buffer & 1 TIME WITH Deionized H2O COUNTERSTAIN IF REQUIRED
15. Visualize tissues under a light microscope.
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MOUNT WITH Aqueous Mounting Medium
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IHC/ICC PROTOCOLS D. IHC Staining of Tissue Sections for Fluorescence Microscopy The following protocol has been developed and optimized by R&D Systems IHC/ICC laboratory using the NorthernLights fluorescent secondary reagents (see page 110). Protocols may need to be modified depending on the type of tissue used. Each investigator should determine the optimal working dilution of an antibody. If using R&D Systems primary antibodies, refer to the product data sheets to obtain approximate working dilutions. For all other reagents follow the manufacturer’s instructions. Caution: DAB is hazardous. Gloves and safety glasses should be worn, and all steps performed inside a fume hood. Please read the protocol in its entirety before starting.
ADDITIONAL SUPPLIES FOR FLUORESCENT IHC STAINING REAGENTS Primary Antibodies Anti-fade Mounting Medium (i-BRITE Plus, Catalog # SF40000, Neuromics, Inc., or equivalent) Blocking buffer: 10% normal donkey serum, 0.3% Triton X-100 Dilution buffer: PBS (1x), 1% bovine serum albumin, 1% normal donkey serum, 0.3% Triton™ X-100, and 0.01% sodium azide NorthernLights Fluorescent Secondary Antibodies/Streptavidin Conjugates (or equivalent) PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4 , pH 7.4 Wash buffer: PBS (1x)
5. Incubate for 1 hour at room temperature with a NorthernLightsconjugated secondary antibody (or equivalent) in dilution buffer designed to recognize the host species of the primary antibody. Alternatively, a NorthernLights-streptavidin conjugate (or equivalent) can be used if the primary antibody is biotinylated.
MATERIALS Coverslips Slides with paraffin-embedded or cryostat tissue sections
7. Mount with an anti-fade mounting medium.
Note: Some tissues may bind non-specifically to biotin-avidin labeling components resulting in high background staining. (See page 5) 6. Wash the slides 3 times for 15 minutes each in wash buffer.
BLOCK THE SLIDE WITH Blocking Buffer (STEP 2)
IHC Staining Using Fluorescence Microscopy
PROCEDURE
INCUBATE FOR 15 MINUTES AT ROOM TEMPERATURE
1. Slide preparation (See Step 1 on page 14) 2. Block the slides using blocking buffer for 15 minutes at room temperature and rinse with wash buffer. 3. Make working dilutions of the primary antibodies in dilution buffer according to manufacturer’s instructions. Add small volumes of these dilutions to the slides and incubate at 4 ˚C overnight, or for 30-60 minutes at room temperature.
RINSE WITH Wash Buffer ADD Primary Antibodies INCUBATE SAMPLE AT 4 ˚C OVERNIGHT OR 1 HOUR AT ROOM TEMPERATURE
Note: Multiple primary antibodies to different antigens can be added in step 5 as long as care is taken to avoid the use of secondary reagents with overlapping spectra. If possible, primary antibodies should also be raised in different species. Please see page 8 for more details. Note: A negative control and/or an isotype-matched control should be included to identify non-specific binding from the secondary antibody. Please see page 5 for more details about IHC/ICC controls.
RINSE 3 TIMES IN Wash Buffer FOR 15 MINUTES ADD Secondary Antibodies
4. Wash the slides 3 times for 15 minutes each in wash buffer.
INCUBATE FOR 1 HOUR AT ROOM TEMPERATURE
RINSE 3 TIMES IN Wash Buffer FOR 15 MINUTES MOUNT WITH Anti-fade Medium Triton X-100 is a trademark of Dow Chemical Corporation.
16
For research use only. Not for use in diagnostic procedures.
IHC/ICC PROTOCOLS E. Antigen Retrieval Using Heat-induced Epitope Retrieval (HIER) Antigen retrieval can reveal epitopes masked during the preparation of tissues for staining. The protocol below describes a technique used by R&D Systems and can be used on both cryostat and paraffin-embedded sections. The protocol can be used with R&D Systems Acidic, Basic, or Universal Antigen Retrieval Reagents. Pretreatment with these solutions may induce a dramatic enhancement of immunoreactivity. However, they also have the potential to affect tissue morphology. The optimal method of antigen retrieval must be determined experimentally. For examples of IHC studies using antigen retrieval, see page 10. Please read the protocol in its entirety before starting.
SUPPLIES REQUIRED REAGENTS Antigen Retrieval Solution (10x): Antigen Retrieval Reagent-Basic (Catalog # CTS013) or Antigen Retrieval Reagent-Acidic (Catalog # CTS014) or Antigen Retrieval Reagent-Universal (Catalog # CTS015) Deionized H2O PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4 , pH 7.4 EQUIPMENT Polypropylene Coplin staining jar (or equivalent) Water bath at 92-95 ˚C MATERIALS Slides with paraffin-embedded or cryostat tissue sections
PROCEDURE 1. Make working dilutions by mixing 1 part of 10x Antigen Retrieval concentrate with 9 parts of deionized water.
HEAT SLIDES IN Antigen Retrieval Solution FOR 2-10 MINUTES 92-95 ˚C
Water bath ALLOW TO COOL AT ROOM TEMPERATURE RINSE WITH Deionized H20 AND THEN WITH PBS
2. Preheat retrieval solution to 92-95 ˚C. This may be achieved by placing a polypropylene Coplin staining jar filled with retrieval solution into a water bath.
AVOID VIGOROUS RINSING
Note: Heating may cause cracking of glass staining dishes. 3. Immerse slides into preheated retrieval solution for 2-10 minutes. Note: Since the effect of antigen retrieval reagents depends on their temperature (90-100 ˚C) and incubation time (up to 30 minutes), optimal conditions should be determined by the individual investigator.
Coplin Staining Jar
Note: Cryostat sections are more sensitive to damage by retrieval solution than paraffin-embedded tissues. To avoid tissue damage, it may be necessary to shorten the incubation time to 2-5 minutes. 4. After the incubation is finished, remove the Coplin jar with retrieval solution and slides from the water bath, and let it cool to room temperature.
BEGIN STAINING PROCEDURE
5. Gently rinse the slides with deionized water and then with PBS. Note: Because tissues may be loosened after the retrieval procedure, avoid vigorous rinsing to prevent detachment from the slides. 6. Tissues are now ready for blocking and primary antibody incubation.
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17
IHC/ICC PROTOCOLS F. Preparation of Coverslips & Cell Fixation for ICC The fundamental principles of IHC experiments using tissue sections also apply to ICC experiments. However, these protocols must be specially adapted for use with cultured cells or cell suspensions. The following protocols describe how to prepare gelatin-coated coverslips and fix cultured cells for ICC experiments. Please read the protocols in its entirety before starting.
Coverslip Preparation Using Gelatin
Preparation & Fixation of Cells on Coverslips
Many cultured cell types do not adhere well to glass coverslips. There are several techniques used to enhance cell adhesion including the use of polyamino acids such as Polyornithine or Poly-L-Lysine, and/or extracellular matrix proteins such as Laminin or Fibronectin. Adding a thin layer of gelatin to a coverslip is also often used to enhance the adhesion of cultured cells to glass.
SUPPLIES REQUIRED REAGENTS Fixative: 2-4% formaldehyde in PBS Wash buffer: 0.1% BSA in 1x PBS Cell culture medium PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4, pH 7.4
SUPPLIES REQUIRED
MATERIALS
REAGENTS Gelatin-coating solution: 0.1% gelatin in deionized H2O
Gelatin-coated coverslips in a 24-well plate
PROCEDURE
MATERIALS Coverslips (Sterilized) Cell culture plate (6- or 24-well)
1. Culture cells by adding 0.5 mL of culture media containing approximately 5,000 cells to the wells of a cell culture plate containing gelatin-coated coverslips.
PROCEDURE 1. Sterilize coverslips and place them into the wells of a 24-well plate. 2. Add 400 µL of gelatin-coating solution and incubate the coverslips for 10 minutes at room temperature. 3. Remove the gelatin-coating solution and air dry the coverslips for 15 minutes. 4. The dried coverslips can now be stored at room temperature until use.
PLATE CELLS ON GELATIN-COATED COVERSLIPS
CULTURE TO DESIRED DENSITY/AGE
2. When cells have reached the desired density/age, remove the culture media from each well and wash twice with PBS. 3. Add 300-400 µL of 2-4% formaldehyde to each well and incubate for 20 minutes at room temperature. 4. Wash the wells twice with PBS and then cover with 400 µL of wash buffer. The coverslips can be stored at 2-8 ˚C for up to 3 months or they may be stained immediately. Note: Fixation can result in hydrophobic cross-linking of tissue proteins. The time, temperature, pH, and fixative used will determine the degree of cross-linking. Once the fixation protocol has been optimized, the same procedure should be used consistently.
ADD 4% Formaldehyde FOR 20 MINUTES AT ROOM TEMPERATURE
WASH 2 TIMES WITH PBS
18
For research use only. Not for use in diagnostic procedures.
ADD 400 µL of Wash Buffer
WASH 2 TIMES WITH PBS
STAIN OR STORE AT 2-8 °C FOR UP TO 3 MONTHS
IHC/ICC PROTOCOLS G. Fluorescent ICC Staining of Cultured Cells The following protocol has been developed and optimized using R&D Systems primary antibodies and NorthernLights fluorescent secondary antibodies (see page 8). This protocol may need to be optimized for each specific ICC experiment. Please read the protocol in its entirety before starting.
SUPPLIES REQUIRED REAGENTS Primary Antibodies Blocking buffer: 10% normal donkey serum, 0.3% Triton X-100 DAPI (4',6-diamidino-2-phenylindole) solution: add 1 μL of 14.3 mM stock for every 5 mL of PBS. Store any unused DAPI at 2-8 ˚C, wrapped in aluminum foil Deionized H2O Dilution buffer: PBS (1x), 1% bovine serum albumin, 1% normal donkey serum, 0.3% Triton™ X-100, and 0.01% sodium azide Anti-fade mounting medium NorthernLights-conjugated secondary reagents (see page 110), or equivalent PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4, pH 7.4 Wash and antibody dilution buffer: 0.1% BSA in PBS (1x)
10. Carefully remove the coverslips from the wells and blot to remove any excess water. Dispense 1 drop of Anti-fade mounting medium onto the microscope slide per coverslip. Mount the coverslip with the cells facing towards the slide. 11. Visualize using a fluorescence microscope and filter sets appropriate for the label used. Slides can also be stored in a slide box at < -20 ˚C for later examination.
BLOCK
MATERIALS Cell-covered coverslips in a 6- or 24-well plate Fine tweezers
RINSE FIXED CELLS WITH Wash Buffer
PROCEDURE Note: This protocol is optimized for cells grown on coverslips in a 6- or 24-well plate but can be adapted accordingly.
INCUBATE SAMPLE 45 MINUTES AT ROOM TEMPERATURE ADD Primary Antibodies
1. Wash the coverslips or slides containing the fixed cells two times in 400 µL of wash buffer
INCUBATE OVERNIGHT AT 2-8 ˚C OR FOR 1 HOUR AT ROOM TEMPERATURE
2. Add 400 µL of blocking buffer and block for 45 minutes at room temperature. 3. Remove blocking buffer. No rinsing is necessary.
ADD Secondary Antibodies
4. Dilute the unconjugated primary antibody (or fluorescenceconjugated primary) in dilution buffer according to the manufacturer’s instructions. Incubate overnight at 2-8 ˚C, or alternatively, incubate at room temperature for 1 hour. Note: Experimentation with both antibody concentration and incubation time may be required to achieve optimal labeling.
NO RINSING IS NECESSARY
RINSE 2 TIMES WITH Wash Buffer
INCUBATE FOR 1 HOUR ADD DAPI Counterstain
5. Wash two times in 400 µL of wash buffer. If using a primary antibody with a direct fluorescent conjugate, go to step 8. 6. Dilute the secondary antibody in dilution buffer according to the manufacturer’s instructions. Add 400 µL to the wells and incubate at room temperature for 1 hour in the dark. From this step forward samples should be protected from light. 7. Wash two times in 400 µL of wash buffer. 8. Add 300 µl of the diluted DAPI solution to each well and incubate 2-5 minutes at room temperature. DAPI binds to DNA and is a convenient nuclear counterstain. It has an absorption maximum at 358 nm and fluoresces blue at an emission maximum of 461 nm.
RINSE 2 TIMES WITH Wash Buffer
INCUBATE 2-5 MINUTES AT ROOM TEMPERATURE RINSE 1 TIME WITH PBS RINSE 1 TIME WITH Deionized H2O REMOVE THE COVERSLIP WITH A FINE TWEEZERS MOUNT WITH THE CELLS FACING THE SLIDE
9. Wash once with PBS and once with water.
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19
IHC/ICC PROTOCOLS H. Preparation of a Cell Smear for Non-adherent Cell ICC One method of preparing non-adherent cells for ICC involves smearing the sample across a gelatincoated slide. This technique forms a monolayer of cells that can be easily visualized. Please read the protocol in its entirety before starting.
SUPPLIES REQUIRED REAGENTS Deionized H2O Fixative: 4% formaldehyde in PBS (1x) PBS (1x): 0.137 M NaCl, 0.05 M NaH2PO4, pH 7.4
FIX CELLS FOR 20 MINUTES AT ROOM TEMPERATURE
SPIN DOWN CELLS FOR 30 SECONDS
RESUSPEND IN 1 mL Deionized H2O
MATERIALS Microfuge tube (1.5 mL) Gelatin-coated microscope slides Hot plate Hydrophobic barrier pen Microfuge
PROCEDURE
SPIN DOWN CELLS FOR 30 SECONDS
1. Fix the cells with 4% formaldehyde for 20 minutes at room temperature by adding formaldehyde directly to the culture media (see page 4) and adjust to approximately 1 x 10 6 cells/mL. 2. Add 1 mL of the cell solution to a 1.5 mL microfuge tube. 3. Spin down the cells for 30 seconds in a microfuge. 4. Pour off the supernatant and resuspend the cell pellet in 1 mL of deionized H2O. 5. Spin down the cells for 30 seconds in the microfuge.
ADD 5 µL Cell Suspension TO GELATIN-COATED SLIDE
6. Pour off the supernatant and resuspend the cell pellet in 200 µL of deionized H2O. 7. Add 5 µL of the cell suspension to a gelatin-coated slide (3 spots per slide) and smear with the side of a pipette tip. 8. Place the slide on a hot plate (low heat setting) and allow the liquid to evaporate. 9. Check the slide under a microscope and make sure that there are no salt crystals. If salt crystals are observed, wash the slide with water.
SMEAR WITH A PIPETTE TIP
10. Surround the cell spot with a hydrophobic barrier using a barrier pen and air dry. 11. The slides can be stored at 2-8 ˚C for up to 3 months or they may be stained immediately.
HEAT TO EVAPORATE THE LIQUID
SURROUND CELLS WITH A Hydrophobic Barrier
20
For research use only. Not for use in diagnostic procedures.
RESUSPEND IN 200 µL Deionized H2O
TROUBLESHOOTING GUIDE Problem: Lack of Staining Possible Source
Test or Action
Lack of antigen.
Check protein expression by in situ hybridization (in some rare cases translation may be blocked even though mRNA is detected).
Antibodies do not work due to improper storage.
Follow storage instructions on the datasheet. In general, aliquot antibodies into smaller volumes sufficient to make a working solution for a single experiment. Store aliquots in a manual defrost freezer (-20 to -70 ˚C) and avoid repeated freeze-thaw cycles.
Inactive primary or secondary antibodies.
Test reporter system independently to assess reagent viability.
Inadequate tissue fixation.
Try increasing the fixation time or try a different fixative.
Tissue overfixation.
Reduce the duration of the immersion or post-fixation steps. If immersion fixation cannot be avoided (for example, collection of postmortem tissues or biopsies in pathology lab), antigens may be unmasked by treatment with antigen retrieval reagents.
Incompatible secondary and primary antibodies.
Use a secondary antibody that will interact with the primary antibody. For example, if the primary antibody was raised in rabbits, use an anti-rabbit secondary antibody.
Antigen was destroyed before incubation with the primary antibody.
If quenching of endogenous peroxidase was done prior to the addition of primary antibodies, block peroxidase after incubation with the primary antibody.
Epitope altered during fixation or embedding procedure.
Try restoring immunoreactivity through various antigen retrieval techniques. Embed tissue at 58 ˚C or below.
Antigen retrieval was ineffective.
Increase the time of treatment or change the treatment solution.
Reagents omitted or used in wrong order.
Repeat staining and confirm that correct reagents are used and are added in the correct order.
Problem: High Background Possible Source
Test or Action
High concentration of primary and or secondary antibodies.
Titer antibody to determine optimal concentration needed to promote a specific reaction of the primary and the secondary antibodies.
Hydrophobic interactions of the antibody and proteins in the tissue.
Lower the ionic strength of the antibody diluent (particularly monoclonal antibodies respond well to reducing the salt concentration).
Non-specific binding of primary and/or secondary reagents to tissues.
Use blocking step just prior to primary antibody incubation (we use 1% bovine serum albumin with 10% normal donkey serum, or non-fat dry milk is another option).
Non-specific binding of secondary antibody.
Use an antibody that has cross-reactive IgG species removed (absorbed against sample species).
Tissue dried out.
Avoid letting the tissue dry during the staining procedure.
Reagents sticking to old or poorly prepared slides.
Start over with freshly prepared or purchased slides.
Background due to ionic interactions.
Increase the ionic strength of the diluent buffer.
Problem: Cell/Tissue Morphology Destroyed Possible Source
Test or Action
Antigen retrieval methods are too harsh.
Empirically determine the conditions that preserve tissue morphology while restoring the immunoreactivity of the antigen.
Tissue sections falling off slide. (more common with frozen sections)
Increase fixation time. Empirically determine an additional or alternative fixative. Use freshly prepared, adequately charged slides.
Tissue section appears torn or folded. Air bubbles under section.
Re-cut sections using a sharp blade, or ignore damaged areas when analyzing the results.
Poor resolution of tissue morphology
Cut thinner tissue sections. Ice crystals may have destroyed morphology of frozen sections.
Underfixation has physically damaged the tissue or cells during the staining process
Increase fixation time and/or add a post-fixation step. Increase the fixative/tissue ratio. Cut smaller pieces of tissue for more thorough immersion fixation.
Autolysis of tissue leading to staining of necrotic debris.
Increase the fixation time, ratio. Consider using cross-linking fixative.
Problem: Staining is Inappropriate
Possible Source
Test or Action
Fixation method is inappropriate for the antigen.
Try a different fixative or increase the fixation time.
Antigen retrieval may be inappropriate for this antigen or tissue.
Try different antigen retrieval conditions.
Electrostatic charge of the antigen has been altered.
Try adjusting the pH or cation concentration of the antibody diluent.
Delay in fixation caused diffusion of the antigen.
Fix tissue promptly. Try a cross-linking fixative rather than organic (alchohol) fixative.
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21
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
2B4/CD244/SLAMF4
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Human
None
Polyclonal
Goat IgG
AF1039
100 µg
$355
None
Polyclonal
Goat IgG
AF1050
100 µg
$355
Mouse
SIZE
PRICE
Biotin
Polyclonal
Goat IgG
BAF1050
50 µg
$380
Human
None
Polyclonal
Goat IgG
AF838
100 µg
$355
Human
Biotin
Polyclonal
Goat IgG
BAF838
50 µg
$380
4-Hydroxynonenal
Multi-species
None
198960
Mouse IgG2B
MAB3249
100 µg
$240
5HT1B
Human
None
499325
Mouse IgG1
MAB5858
100 µg
$240
None
Polyclonal
Sheep IgG
AF4975
100 µg
$355
None
524731
Mouse IgG1
MAB4975
100 µg
$240
None
Polyclonal
Sheep IgG
AF5049
100 µg
$355
None
2E2
Mouse IgG2B
4354-MC-050
50 µL
$415
None
105
Mouse IgM
MAB1416
100 µg
$295
4-1BB/TNFRSF9/CD137
5T4
Human Mouse
5T4 in Human Placenta. 5T4 was detected in paraffin-embedded human placenta sections using antihuman 5T4 affinity-purified polyclonal antibody (Catalog # AF4975). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
8-oxo-dG A2B5
Human/Mouse/Rat/Chicken
A2B5 in Human differentiating NTera2 Cells. NTera2 neuronal carcinoma cells were differentiated with retinoic acid for 10 days. A2B5 was detected using anti-human/mouse/rat/chicken A2B5 monoclonal antibody (Catalog # MAB1416). The cells were stained using NorthernLights-557 anti-mouse IgG secondary antibody (Catalog # NL007; red) and the nuclei counterstained with DAPI (blue).
a1-Acid Glycoprotein a-2-Macroglobulin
a2u-Globulin
A33
Human Human
Rat
Human Mouse
ABCG2
ACE/CD143
Human
Human
Mouse
None
386131
Mouse IgG2A
MAB3694
100 µg
$240
None
Polyclonal
Goat IgG
AF1938
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1938
50 µg
$380
None
Polyclonal
Goat IgG
AF586
100 µg
$355
Biotin
129736
Mouse IgG1
BAM586
250 µg
$380
None
129736
Mouse IgG1
MAB586
500 µg
$325
None
Polyclonal
Goat IgG
AF3080
100 µg
$355
None
402104
Rat IgG2A
MAB3080
100 µg
$240
None
Polyclonal
Goat IgG
AF2756
100 µg
$355
Biotin
5D3
Mouse IgG2B
BAM995
250 µg
$380
None
5D3
Mouse IgG2B
MAB995
500 µg
$325
None
Polyclonal
Goat IgG
AF929
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF929
50 µg
$380
None
Polyclonal
Goat IgG
AF1513
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1513
50 µg
$380
ACE in Human Kidney. Angiotensin Converting Enzyme (ACE) was detected in paraffin-embedded human kidney tissue sections using anti-human ACE polyclonal antibody (Catalog # AF929). Tissues were stained using the Goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
22
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
ACE-2
SPECIES
Human
Mouse
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF933
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF933
50 µg
$380
None
171606
Mouse IgG2A
MAB933
500 µg
$325
None
Polyclonal
Goat IgG
AF3437
100 µg
$355
Polyclonal
Sheep IgG
AF4000
100 µg
$355
ACE-2 in Mouse Kidney. Angiotensin Converting Enzyme-2 (ACE-2) was detected in a cryostat section of mouse kidney using anti-mouse affinity-purified ACE-2 polyclonal antibody (Catalog # AF3437; red). The tissue was counterstained green.
Actin
Human/Mouse/Rat
None
Activin A bA subunit
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF338
100 µg
$355
Activin C bC subunit
Human
None
Polyclonal
Goat IgG
AF1629
100 µg
$355
Activin RIA/ALK-2
Human
None
Polyclonal
Goat IgG
AF637
100 µg
$355
None
71412
Mouse IgG1
MAB637
500 µg
$325
Activin RIB/ALK-4
Mouse
None
Polyclonal
Goat IgG
AF1477
100 µg
$355
Activin RIIA
Human
None
Polyclonal
Goat IgG
AF340
100 µg
$355
Activin RIIB
Human
None
Polyclonal
Goat IgG
AF339
100 µg
$355
ADAM8 Ectodomain
Human
None
Polyclonal
Goat IgG
AF1031
100 µg
$355
ADAM9 Ectodomain ADAM10 Ectodomain
ADAM15 Ectodomain
Mouse
None
Polyclonal
Goat IgG
AF949
100 µg
$355
Human
None
Polyclonal
Total Goat IgG
AB936
1 mg
$355
Mouse
None
Polyclonal
Total Goat IgG
AB946
1 mg
$355
None
Polyclonal
Goat IgG
AF935
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF935
50 µg
$380
None
23G9
Mouse IgG1
MAB935
500 µg
$325
None
Polyclonal
Goat IgG
AF945
100 µg
$355
504425
Mouse IgG2A
MAB4974
100 µg
$240
539220
Mouse IgG1
MAB5380
100 µg
$240
Human
Mouse
ADAM15 Expression in Mouse Cerebellum. A dis integin and mettalloprotease domain 15 (ADAM15) was detected in cryostat sections of mouse cerebellar Purkinje cells using anti-mouse ADAM15 polyclonal antibody (Catalog # AF945; red). The tissue was counterstained green.
ADAM23 aa 60-585
Human
None
ADAM23 in Human Spleen. A disintegrin and metalloprotease domain 23 (ADAM23) was detected in paraffin-embedded human spleen sections using anti-human ADAM23 monoclonal antibody (Catalog # MAB4974). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
ADAM32 aa 17-476
Human
None
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23
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE ADAMTS1 Propeptide
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human
None
Polyclonal
Goat IgG
AF3079
100 µg
$355
Polyclonal
Goat IgG
AF1119
100 µg
$355
ADAMTS1 in Human Placenta. A disintegrin-like and mettaloprotease with thrombospondin motifs (ADAMTS1) was detected in a paraffin-embedded human placenta section using anti-human ADAMTS1 affinity-purified polyclonal antibody (Catalog # AF3079). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Adiponectin/Acrp30 Adiponutrin/ADPN
Mouse
None
Rat
None
Polyclonal
Goat IgG
AF3100
100 µg
$355
Human
None
Polyclonal
Sheep IgG
AF5208
100 µg
$355
Polyclonal
Chicken IgY
AF1369
100 µg
$355 $240
ADPN in Human Adipocytes. Adiponutrin (ADPN) was detected in human adipocytes using anti-human ADPN affinity-purified polyclonal antibody (Catalog # AF5208). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and the nuclei counterstained with DAPI (blue).
None
Human a-Fetoprotein/AFP
Aggrecan G1-IGD-G2 Domains
AgRP/ART
None
189506
Mouse IgG1
MAB1369
100 µg
Human/Mouse
None
189502
Mouse IgG1
MAB1368
100 µg
$240
Mouse
None
Polyclonal
Goat IgG
AF5369
100 µg
$355
None
Polyclonal
Goat IgG
AF1220
100 µg
$355
None
179509
Mouse IgG2B
MAB1220
500 µg
$325
Human
None
Polyclonal
Goat IgG
AF704
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF634
100 µg
$355
None
Polyclonal
Rabbit IgG
AF1457
100 µg
$285
None
Polyclonal
Sheep IgG
AF5824
100 µg
$355 $240
Human
AgRP in Mouse Thalamus. Agouti-Related Protein (AgRP) was detected in a cryostat section of mouse thalamus using anti-mouse AgRP monoclonal antibody (MAB634; red). The tissue was counterstained green.
AIF
Human/Mouse/Rat
a-Internexin
Human
None
529724
Mouse IgG1
MAB4844
100 µg
Akt Pan Specific
Human/Mouse/Rat
None
281046
Mouse IgG2B
MAB2055
100 µg
$240
Phospho-Akt (S473) Pan Specific
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF887
100 µg
$285
Akt1
Human
None
302407
Mouse IgG1
MAB17751
100 µg
$240
Human
None
302501
Mouse IgG1
MAB23152
100 µg
$240
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF23151
100 µg
$355
Human/Mouse
None
188835
Mouse IgG2A
MAB1455
100 µg
$240
None
Polyclonal
Goat IgG
AF1172
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1172
50 µg
$380
None
Polyclonal
Goat IgG
AF577
100 µg
$355
Biotin
118311
Mouse IgG2A
BAM5771
100 µg
$240
Akt2 Serum Albumin ALCAM/CD166
Mouse
ALK-7
Rat
24
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Alkaline Phosphatase/ALPL
SPECIES Human/Mouse/Rat
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Biotin
B4-78
Mouse IgG1
BAM1448
100 µg
$240
None
B4-78
Mouse IgG1
MAB1448
100 µg
$240
Alkaline Phosphatase and Oct-3/4 in Human BG01V Embryonic Stem Cells. Alkaline Phosphatase was detected in the human BG01V embryonic stem cell line using anti-human/mouse/rat Alkaline Phosphatase monoclonal antibody (Catalog # MAB1448). Oct-3/4 was detected using anti-human Oct-3/4 polyclonal antibody (Catalog # AF1759). The cells were stained for Alkaline Phosphatase using NorthernLights-557 anti-mouse secondary antibody (Catalog # NL007; pseudocolored green) and stained for Oct-3/4 using NorthernLights-637 anti-goat secondary antibody (Catalog # NL002; red).
AMIGO2
Human
None
273610
Mouse IgG3
MAB20801
100 µg
$240
AMIGO3
Mouse
None
371301
Rat IgG2A
MAB2375
100 µg
$240
AMIGO3 in Mouse Cerebellum. Amphoterin-induced Gene and ORF 3 (AMIG03) was detected in Purkinje neuron cell bodies in a cryostat section of mouse cerebellum using anti-mouse AMIG03 monoclonal antibody (Catalog # MAB2375).
Aminopeptidase N/ANPEP
Human
None
498001
Mouse IgG2A
MAB3815
100 µg
$240
Mouse
None
Polyclonal
Goat IgG
AF2335
100 µg
$355
AF4647
100 µg
$355
Aminopeptidase N/CD13 in Human Liver Cancer. Aminopeptidase N (APN) was detected in paraffinembedded human liver cancer sections using antihuman APN monoclonal antibody (Catalog # MAB3815). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Cata log # CTS002; brown) and counterstained with hematoxylin (blue).
Aminopeptidase O/ONPEP
Human
None
Polyclonal
Goat IgG
Aminopeptidase P1/XPNPEP1
Human
None
409124
Mouse IgG1
MAB29701
100 µg
$240
None
Polyclonal
Total Goat IgG
AB-262-NA
1 mg
$355
None
Polyclonal
Goat IgG
AF262
100 µg
$355
Amphiregulin
AMPKa1 aa 340-550
Human Mouse
None
Polyclonal
Goat IgG
AF989
100 µg
$355
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF3197
100 µg
$355
AMPKa2 aa 340-552
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF2850
100 µg
$355
AMPKb1
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF2854
100 µg
$355
Androgen R/NR3C4
Human
None
H7507
Mouse IgG2A
PP-H7507-00
100 µg
$380
Angiogenin
Human
None
Polyclonal
Goat IgG
AF265
100 µg
$355
Angiopoietin-1
Human
None
Polyclonal
Goat IgG
AF923
100 µg
$355
None
171718
Mouse IgG2B
MAB923
500 µg
$325
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25
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Angiopoietin-2
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human
None
Polyclonal
Goat IgG
AF623
100 µg
$355
Polyclonal
Goat IgG
AF738
100 µg
$355
Angiopoietin-2 in Human Gastrointestinal Cancer. Angiopoietin-2 was detected in paraffin-embedded human gastrointestinal cancer sections using anti-human Angiopoietin-2 affinity-purified polyclonal antibody (Catalog # AF623). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Angiopoietin-3
Mouse
None
Angiopoietin-4
Human
None
Polyclonal
Goat IgG
AF964
100 µg
$355
Angiopoietin-like 1
Human
None
Polyclonal
Goat IgG
AF2204
100 µg
$355
Angiopoietin-like 2
Mouse
None
Polyclonal
Goat IgG
AF1444
100 µg
$355
Angiopoietin-like 3
Mouse
None
Polyclonal
Goat IgG
AF136
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF136
50 µg
$380
ANP
Human
None
361101
Rat IgG2B
MAB3366
100 µg
$240
AP-2g
Human
None
Polyclonal
Goat IgG
AF5059
100 µg
$355
APC C-Terminus
Human
None
Polyclonal
Goat IgG
AF3695
100 µg
$355
APJ
Human
None
72133
Mouse IgG3
MAB856
500 µg
$325
APLP-1
Human
None
Polyclonal
Goat IgG
AF3129
100 µg
$355
None
354008
Mouse IgG2B
MAB3129
100 µg
$240
None
Polyclonal
Goat IgG
AF3556
100 µg
$355
None
369717
Mouse IgG2B
MAB4124
100 µg
$240
Apolipoprotein B/ApoB
Human
APP Pan Specific
Human
None
Polyclonal
Goat IgG
AF1168
100 µg
$355
Phospho-APP (T668)
Human/Mouse
None
304904
Mouse IgG1
MAB2508
100 µg
$240
Phospho-Amyloid Precursor Protein (668T) in Human Alzheimer’s Disease Brain. Amyloid precursor protein (APP) phosphorylated at T668 was detected in paraffin-embedded human Alzheimer’s disease brain sections using anti-human phospho-APP monoclonal antibody (Catalog # MAB2508). Tissue was stained using anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
ARC aa 176-195
Human/Mouse/Rat
b-Arrestin 1
Human
b-Arrestin 2
Human Human
Artemin
Mouse
None
Polyclonal
Rabbit IgG
AF636
100 µg
$285
None
Polyclonal
Goat IgG
AF4560
100 µg
$355
None
425817
Rat IgG2A
MAB45601
100 µg
$240
None
Polyclonal
Goat IgG
AF4564
100 µg
$355
None
Polyclonal
Goat IgG
AF2589
100 µg
$355
None
Polyclonal
Goat IgG
AF1085
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1085
50 µg
$380
None
185234
Rat IgG2A
MAB10851
500 µg
$325
Arylsulfatase A/ARSA
Human
None
Polyclonal
Goat IgG
AF2485
100 µg
$355
ASK1 aa 1011-1196
Human
None
Polyclonal
Sheep IgG
AF3575
100 µg
$355
Phospho-ATF2 (S490/S498)
Human/Rat
None
Polyclonal
Rabbit IgG
PPS064
100 µL
$355
Axl
Human
None
Polyclonal
Goat IgG
AF154
100 µg
$355
26
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Phospho-Axl (Y779)
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human
None
Polyclonal
Rabbit IgG
AF2228
100 µg
$285
RA3-6B2
Rat IgG2A
BAM1217
250 µg
$380
Phospho-Axl (Y779) in Human Stomach. PhosphoAxl was detected in paraffin-embedded human stomach sections using anti-human/mouse phospho-Axl affinity-purified polyclonal antibody (Catalog # AF2228). Tissue was stained using anti-rabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue).
B220/CD45R
Mouse
Human B7-1/CD80
Mouse Rat Human
B7-2/CD86
Rat
Biotin None
RA3-6B2
Rat IgG2A
MAB1217
500 µg
$325
None
Polyclonal
Goat IgG
AF140
100 µg
$355
None
37711
Mouse IgG1
MAB140
500 µg
$325
None
Polyclonal
Goat IgG
AF740
100 µg
$355
None
Polyclonal
Goat IgG
AF1214
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1214
50 µg
$380
None
Polyclonal
Goat IgG
AF-141-NA
200 µg
$230
None
Polyclonal
Goat IgG
AF1340
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1340
50 µg
$380
B7-H1/PD-L1
Mouse
None
Polyclonal
Goat IgG
AF1019
100 µg
$355
B7-H2
Mouse
None
Polyclonal
Goat IgG
AF158
100 µg
$355
B7-H3
Human
None
Polyclonal
Goat IgG
AF1027
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1027
50 µg
$380
BACE-1 Ectodomain
Human/Mouse
None
137612
Mouse IgG1
MAB931
500 µg
$325
BACE-2 Ectodomain
Human
None
391017
Rat IgG2A
MAB4097
100 µg
$240
Human
None
Polyclonal
Goat IgG
AF124
100 µg
$355
BAFF/BLyS/TNFSF13B
Mouse
Biotin
Polyclonal
Goat IgG
BAF2106
50 µg
$380
BAI1
Human
None
542515
Mouse IgG1
MAB4969
100 µg
$240
BAI3
Human
None
409633
Mouse IgG1
MAB39651
100 µg
$240
BAMBI/NMA
Human
None
Polyclonal
Goat IgG
AF921
100 µg
$355
Bax aa 12-33
Human/Mouse
None
Polyclonal
Rabbit IgG
AF820
30 µg
$285
Bax Minus C-Terminus
Human
None
Polyclonal
Total Goat IgG
AB820
400 µg
$395
Bcl-2 aa 1-208
Human/Mouse
None
Polyclonal
Goat IgG
AF810
100 µg
$355
Bcl-w aa 1-172
Human/Mouse
None
Polyclonal
Goat IgG
AF8241
100 µg
$355
Bcl-xL aa 3-14
Human/Mouse/Rat
None
YTH-2H12
Mouse IgG2A
2300-MC-100
100 µg
$285
None
Polyclonal
Chicken IgY
AF248
100 µg
$355
BDNF
Human
Biotin
Polyclonal
Chicken IgY
BAF248
50 µg
$380
None
35928
Mouse IgG1
MAB248
500 µg
$325
BDNF in Rat Spinal Cord and Dorsal Root Ganglia. Brain-derived neurotrophic factor (BDNF) was detected in rat dorsal root ganglion using anti-human BDNF affinity-purified polyclonal antibody (Catalog # AF248; red) and counterstained green.
Human Betacellulin/BTC
bIG-H3
Mouse Mouse
None
Polyclonal
Goat IgG
AF-261-NA
100 µg
$355
None
Polyclonal
Goat IgG
AF1025
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1025
50 µg
$380
None
Polyclonal
Sheep IgG
AF2559
100 µg
$355
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
27
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Biglycan
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human
None
Polyclonal
Goat IgG
AF2667
100 µg
$355
Polyclonal
Sheep IgG
AF5474
100 µg
$355
Biglycan in Human Skin. Biglycan was detected in paraffin-embedded human skin sections using antihuman Biglycan affinity-purified polyclonal antibody (Catalog # AF2667). Tissue was stained using antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
BIK
Human
None
BMI-1
Human
None
Polyclonal
Goat IgG
AF3334
100 µg
$355
BMP-1/PCP
Human
None
Polyclonal
Goat IgG
AF1927
100 µg
$355
Pro BMP-2
Human
None
253717
Mouse IgG2A
MAB2260
500 µg
$325
BMP-2/BMP-4
Human
None
Polyclonal
Goat IgG
AF355
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF355
50 µg
$380
BMP-3
Human
None
Polyclonal
Goat IgG
AF113
100 µg
$355
BMP-3b/GDF-10
Human
BMP-5
Human
BMP-6
Human
None
Polyclonal
Goat IgG
AF1543
100 µg
$355
None
Polyclonal
Goat IgG
AF615
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF615
50 µg
$380
None
Polyclonal
Goat IgG
AF507
100 µg
$355
None
164311
Mouse IgG2B
MAB3541
500 µg
$325
None
164313
Mouse IgG1
MAB3542
500 µg
$325
BMP-7
Human
BMP-8
Human
None
Polyclonal
Goat IgG
AF1073
100 µg
$355
Human
None
317809
Rat IgG2A
MAB2925
100 µg
$240
MAB24061
100 µg
$240
BMP-15/GDF-9B aa 268-392
BMP-15/GDF-9b in Mouse Ovary. Bone Morphogenetic Protein-15 (BMP-15) was detected in a frozen section of adult mouse ovary using anti-human BMP15 monoclonal antibody (Catalog # MAB2925). Cells were stained using NorthernLights-557 goat anti-rat IgG secondary antibody (red; Catalog # NL013) and counterstained with DAPI (blue).
BMPR-IA/ALK-3
Human
None
550607
Mouse IgG2B
BMPR-IB/ALK-6
Human
None
477914
Mouse IgG2B
MAB5051
100 µg
$240
None
Polyclonal
Goat IgG
AF811
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF811
50 µg
$380
BMPR-II
Human
BMPR-II in Mouse Embryo. BMPR-II was detected in a frozen section of E15 mouse ribs using anti-human BMPR-II affinity-purified polyclonal antibody (Catalog # AF811; green).
BNP Precursor
Human
None
370124
Rat IgG2B
MAB36041
100 µg
$240
BOC
Mouse
None
Polyclonal
Goat IgG
AF2385
100 µg
$355
28
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Brachyury
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Human
None
Polyclonal
Goat IgG
AF2085
Brachyury in Differentiated Human Embryonic Stem cells. Brachyury was detected in differentiated human Embryonic Stem cells using anti-human Brachyury affinity-purified polyclonal antibody (Catalog # AF2085). Cells were stained using an anti-goat secondary antibody (green) and counterstained with DAPI (blue). Courtesy of Dr. Frank Solder from the National Institute of Neurological Disorders and Stroke & Stem Cell Unit at NIH.
Brachyury NorthernLights-conjugated
Human
NL557
SIZE
PRICE
100 µg
$355
Brachyury in Embryonic Mouse Notochord. Brachyury was detected in E9.5 mouse notochord using anti-human Brachyury affinity-purified polyclonal antibody (Catalog # AF2085). The cells were stained using NorthernLights-557 secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
Polyclonal
Goat IgG
NL2085R
500 µL
$350 $355
Human
None
Polyclonal
Goat IgG
AF2210
100 µg
Human/Mouse/Rat
None
265402
Mouse IgG2A
MAB2210
100 µg
$240
Phospho-BRCA1 (S1423)
Human
None
Polyclonal
Rabbit IgG
AF1386
100 µg
$285
BRCA2
Human
None
234403
Mouse IgG1
MAB2476
100 µg
$240
None
Polyclonal
Sheep IgG
AF4009
100 µg
$355
Biotin
Polyclonal
Sheep IgG
BAF4009
50 µg
$380
BRCA1
Brevican
Human
Brk aa 1-70
Human
None
Polyclonal
Goat IgG
AF3966
100 µg
$355
BSRP-A
Mouse
None
529805
Rat IgG2A
MAB4916
100 µg
$240
AF2379
100 µg
$355
BSRP-A in Mouse Hippocampus. Brain-specific receptor-like protein A (BSRP-A) was detected in a cryostat section of mouse hippocampus using anti mouse BSRP-A monoclonal antibody (Catalog # MAB4916). The tissue was stained using the anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).
C1q R1/CD93 C4.4A/LYPD3
Cadherin-6/KCAD
Human
None
Polyclonal
Goat IgG
Mouse
None
223437
Rat IgG2B
MAB1696
100 µg
$240
Mouse
None
Polyclonal
Goat IgG
AF5567
100 µg
$355
None
Polyclonal
Sheep IgG
AF2715
100 µg
$355
None
427909
Mouse IgG1
MAB2715
100 µg
$240
None
427903
Mouse IgG3
MAB27151
100 µg
$240
Human
K-Cadherin in Human Liver Cancer. K-Cadherin was detected in paraffin-embedded human liver cancer sections using anti-human K-cadherin monoclonal antibody (Catalog # MAB27151). The tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
Cadherin-11
Human
None
Polyclonal
Goat IgG
AF1790
100 µg
$355
Cadherin-12
Human
None
343621
Rat IgG1
MAB2240
100 µg
$240
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
29
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Cadherin-13
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human
None
Polyclonal
Goat IgG
AF3264
100 µg
$355
None
Polyclonal
Goat IgG
AF1032
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1032
50 µg
$380
None
141713
Mouse IgG1
MAB1032
500 µg
$325
None
Polyclonal
Goat IgG
AF648
100 µg
$355
None
180215
Mouse IgG2B
MAB1838
500 µg
$325
None
180224
Mouse IgG2B
MAB18381
100 µg
$240
None
Polyclonal
Goat IgG
AF748
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF748
50 µg
$380
None
114420
Rat IgG2A
MAB7481
100 µg
$240
Polyclonal
Sheep IgG
AF1388
100 µg
$355
Cadherin-13 in Human Lung Cancer Cells. Cadherin-13 was detected in NCI-H460 human lung cancer cell line using anti-human Cadherin-13 affinitypurified polyclonal antibody (Catalog # AF3264). Cells were stained using NorthernLights-557 antigoat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
Cadherin-17
Human
Human E-Cadherin Mouse
E-Cadherin and SOX2 in the BG01V Human Embryonic Stem Cell Line. E-Cadherin and SOX2 were detected in human BG01V cells using antihuman E-Cadherin affinity-purified polyclonal antibody (Catalog # AF648; red) and anti-human/ mouse SOX2 monoclonal antibody (Catalog # MAB2018; green). Cells were stained using anti-goat NorthernLights-557 (Catalog # NL001) and antimouse NorthernLights-493 (Catalog # NL009). Cells were counterstained with DAPI (blue).
N-Cadherin
Human
None
N-Cadherin in Human Hippocampus. N-Cadherin was detected in a paraffin-embedded section of human hippocampus using anti-human N-Cadherin affinity-purified polyclonal antibody (Catalog # AF1388). The tissue was stained using anti-sheep HRP-DAB detection reagents (brown) and counterstained with hematoxylin (blue).
Human P-Cadherin
VE-Cadherin
Calbindin D
Mouse
Human
Human
None
104805
Mouse IgG1
MAB861
500 µg
$325
None
Polyclonal
Goat IgG
AF761
100 µg
$355
None
106020
Rat IgG2A
MAB761
500 µg
$325
None
Polyclonal
Goat IgG
AF938
100 µg
$355
None
123413
Mouse IgG2B
MAB9381
100 µg
$240
None
Polyclonal
Goat IgG
AF3320
100 µg
$355
None
401025
Mouse IgG2B
MAB3320
100 µg
$240
Calbindin D in Rat Hypothalamus. Calbindin D was detected in a frozen rat hypothalamus section using anti-human calbindin D monoclonal antibody (Catalog # MAB3320). Tissue was stained using anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
30
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Calretinin
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF5065
100 µg
$355
AF5244
100 µg
$355
Calretinin/Calbindin-2 in Human Brain Cortex. Calretinin was detected in paraffin-embedded human brain cortex sections using anti-human/mouse/ rat Calretinin affinity-purified polyclonal antibody (Catalog # AF5065). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Calsyntenin-3
Human
None
Polyclonal
Sheep IgG
CaM Kinase IIa
Human
None
525501
Mouse IgG2B
MAB5584
100 µg
$240
CaM Kinase IId aa 402-478
Human
None
Polyclonal
Sheep IgG
AF4176
100 µg
$355
CaMKIIδ in Human Cerebral Cortex. Calcium/ Calmodulin-dependent protein kinase type II delta (CaMKIId) was detected in paraffin-embedded hum a n cerebral cortex tissue sections using anti-human CaMKIId affinity-purified polyclonal antibody (Catalog # AF4176). Tissues were stained using antisheep HRP-DAB detection (brown) and counterstained with hematoxylin (blue).
cAMP
Multi-species
None
250532
Mouse IgG1
MAB2146
500 µg
$325
Cannabinoid R2/CB2/CNR2
Human
None
352114
Mouse IgG2A
MAB3655
100 µg
$240
CAR/NR1I3
Human
None
N4111
Mouse IgG1
PP-N4111-00
100 µg
$380
Carbonic Anhydrase II
Human
None
Polyclonal
Sheep IgG
AF2184
100 µg
$355
None
Polyclonal
Goat IgG
AF2186
100 µg
$355
None
310413
Mouse IgG2B
MAB2186
100 µg
$240
None
Polyclonal
Goat IgG
AF2414
100 µg
$355
Carbonic Anhydrase IV
Human Mouse
Carbonic Anhydrase IX
Carnosine Dipeptidase 1/CNDP1
Human
None
Polyclonal
Goat IgG
AF2188
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF2344
100 µg
$355
None
Polyclonal
Goat IgG
AF2489
100 µg
$355
None
352216
Mouse IgG1
MAB2489
100 µg
$240
None
Polyclonal
Goat IgG
AF163
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF163
50 µg
$380
Human
CART
Human
CART aa 28-116
Human
None
113612
Mouse IgG2B
MAB163
500 µg
$325
Active Caspase-3 aa 163-175
Human/Mouse
None
Polyclonal
Rabbit IgG
AF835
50 µg
$285
Cleaved Caspase-3 (Asp175)
Human/Mouse
None
269518
Rabbit IgG
MAB835
50 µg
$270
Caspase-9 aa 1-134
Human
None
LAP6
Mouse IgG1
MAB8301
100 µg
$240
Caspase-9 aa 139-330
Human
None
Polyclonal
Goat IgG
AF8301
100 µg
$355
Human
None
Polyclonal
Sheep IgG
AF5145
100 µg
$355
Caspr2
Caspr2 in Mouse Neurons. Caspr2 was detected in a frozen section of adult mouse brain using anti-human Caspr2 affinity-purified polyclonal antibody (Catalog # AF5145). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
31
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES Human
b-Catenin
Human/Mouse/Rat
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
196618
Mouse IgG3
MAB13291-050
50 µg
$150
None
196618
Mouse IgG3
MAB13291-100
100 µg
$240
None
Polyclonal
Goat IgG
AF1329
100 µg
$355
None
Polyclonal
Goat IgG
AF1049
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1049
50 µg
$380
None
191424
Rat IgG2B
MAB11231
500 µg
$325
None
Polyclonal
Goat IgG
AF953
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF953
50 µg
$380
None
Polyclonal
Goat IgG
AF965
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF965
50 µg
$380
None
Polyclonal
Goat IgG
AF1071
100 µg
$355
None
Polyclonal
Goat IgG
AF1034
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1034
50 µg
$380
None
Polyclonal
Goat IgG
AF1014
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1014
50 µg
$380
None
Polyclonal
Goat IgG
AF1029
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1029
50 µg
$380
None
Polyclonal
Goat IgG
AF1294
100 µg
$355
b-Catenin in Human Kidney Cancer. b-Catenin was detected in paraffin-embedded human kidney cancer sections using anti-human/mouse/rat b-Catenin affinity-purified polyclonal antibody (Catalog # AF1329). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Cathepsin A/Lysosomal Carboxypeptidase A
Human Mouse Human
Cathepsin B Mouse Human Cathepsin C/DPPI
Mouse
Human Cathepsin D Mouse
Human Cathepsin E Mouse
Cathepsin F
Human Mouse
Biotin
Polyclonal
Goat IgG
BAF1294
50 µg
$380
None
Polyclonal
Goat IgG
AF1130
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1130
50 µg
$380
None
Polyclonal
Goat IgG
AF2075
100 µg
$355
None
237208
Mouse IgG1
MAB2075
500 µg
$325
None
Polyclonal
Goat IgG
AF2544
100 µg
$355
Polyclonal
Goat IgG
AF1013
100 µg
$355
Cathepsin F in Human Heart. Cathepsin F was detected in paraffin-embedded human heart tissue sections using anti-human Cathepsin F monoclonal antibody (Catalog # MAB2075). Tissues were stained using anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
Cathepsin H
32
Mouse
None
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF952
100 µg
$355
None
204106
Rat IgG2B
MAB952
500 µg
$325
Human/Mouse
None
204101
Rat IgG2A
MAB9521
500 µg
$325
Mouse
None
Polyclonal
Goat IgG
AF1515
100 µg
$355
None
Polyclonal
Goat IgG
AF1174
100 µg
$355
None
Polyclonal
Goat IgG
AF1183
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1183
50 µg
$380
None
Polyclonal
Goat IgG
AF1080
100 µg
$355
None
182016
Mouse IgG1
MAB1080
500 µg
$325
None
Polyclonal
Goat IgG
AF934
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF934
50 µg
$380
None
Polyclonal
Goat IgG
AF1033
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1033
50 µg
$380
Human Cathepsin L
Cathepsin L in Mouse Ovary. Cathepsin L was detected in frozen sections of mouse ovary using anti-mouse Cathepsin L affinity-purified polyclonal antibody (Catalog # AF1515). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Cathepsin O
Human
Cathepsin S
Human
Cathepsin V
Human
Human Cathepsin X/Z/P Mouse CBP
Human/Mouse/Rat
None
257003
Mouse IgG2B
MAB2676
100 µg
$240
Ccd1/DIXDC1
Mouse
None
Polyclonal
Goat IgG
AF5599
100 µg
$355
Human/Rat
None
525222
Mouse IgG1
MAB4499
100 µg
$240
None
Polyclonal
Rabbit IgG
AF2680
50 µg
$285
None
Polyclonal
Goat IgG
AF1774
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1774
50 µg
$380
None
280702
Mouse IgG1
MAB28171
500 µg
$325
None
23002
Mouse IgG2B
MAB2791
500 µg
$325
Biotin
Polyclonal
Goat IgG
BAF270
50 µg
$380
None
93321
Mouse IgG1
MAB270
500 µg
$325
None
Polyclonal
Goat IgG
AF-450-NA
100 µg
$355
None
Polyclonal
Goat IgG
AF-271-NA
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF271
None
Polyclonal
Goat IgG
AF-451-NA
CCK4 aa 31-199
CCK4 in Human Colon Cancer. Colon carcinoma kinase 4 (CCK4) was detected in a paraffin-emedded section of human colon cancer using anti-human CCK4 monoclonal antibody (Catalog # MAB4499). The tissue was stained using the anti-mouse HRPDAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
CCK-A R aa 256-267
CCL2/JE/MCP-1
Human/Mouse/Rat
Canine
Human
CCL3/MIP-1a
Human Mouse Human
CCL4/MIP-1b Mouse Human CCL5/RANTES
Mouse
50 µg
$380
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF451
50 µg
$380
None
Polyclonal
Goat IgG
AF-278-NA
100 µg
$355
None
Polyclonal
Goat IgG
AF478
100 µg
$355
None
53405
Rat IgG2A
MAB478
500 µg
$325
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
33
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES Human
CCL11/Eotaxin Mouse
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF-320-NA
100 µg
$355
None
43911
Mouse IgG1
MAB320
500 µg
$325
None
Polyclonal
Goat IgG
AF-420-NA
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF420
50 µg
$380
Biotin
42285
Rat IgG2A
BAM420
250 µg
$380
None
42285
Rat IgG2A
MAB420
500 µg
$325
None
Polyclonal
Goat IgG
AF802
100 µg
$355
CCL16/HCC-4
Human
None
70218
Mouse IgG1
MAB328
500 µg
$325
CCL17/TARC
Human
None
Polyclonal
Goat IgG
AF364
100 µg
$355
Human
None
54909
Mouse IgG2B
MAB361
500 µg
$325
Mouse
None
Polyclonal
Goat IgG
AF880
100 µg
$355
None
Polyclonal
Goat IgG
AF360
100 µg
$355
CCL19/MIP-3b
CCL20/MIP-3a
Human
Biotin
Polyclonal
Goat IgG
BAF360
50 µg
$380
CCL21/6Ckine
Human
None
Polyclonal
Goat IgG
AF366
100 µg
$355
CCL24/Eotaxin-2/MPIF-2
Human
None
Polyclonal
Goat IgG
AF343
100 µg
$355 $355
CCL25/TECK
Mouse
None
Polyclonal
Goat IgG
AF-481-NA
100 µg
CCL25/TECK
Mouse
None
89827
Rat IgG2A
MAB4811
500 µg
$325
CCL26/Eotaxin-3
Human
None
Polyclonal
Goat IgG
AF653
100 µg
$355
None
Polyclonal
Goat IgG
AF376
100 µg
$355
None
124308
Mouse IgG1
MAB376
500 µg
$325
None
124302
Mouse IgG2A
MAB3761
500 µg
$325
None
Polyclonal
Goat IgG
AF725
100 µg
$355
Biotin
68706
Rat IgG2A
BAM7251
250 µg
$380
None
68706
Rat IgG2A
MAB7251
500 µg
$325
Human CCL27/CTACK Mouse
CCL27/CTACK in Normal Human Skin. CCL27/ CTACK was detected in a frozen tissue section of normal human skin using anti-human CCL27/CTACK affinity-purified polyclonal antibody (Catalog # AF376). Tissues were stained using the anti-goat HRP-AEC Cell and Tissue Staining Kit (Catalog # CTS009; red) and counterstained with hematoxylin (blue).
CCR1
Human
None
53504
Mouse IgG2B
MAB145
500 µg
$325
CCR3
Human
None
61828
Rat IgG2A
MAB155
500 µg
$325
CCR5
Human
None
45523
Mouse IgG2B
MAB181
500 µg
$325 $325
CCR6
CCR7
None
45549
Mouse IgG2B
MAB183
500 µg
Human
None
53103
Mouse IgG2B
MAB195
500 µg
$325
Mouse
None
140706
Rat IgG2A
MAB590
500 µg
$325
Human
None
150503
Mouse IgG2A
MAB197
500 µg
$325
Mouse
None
4B12
Rat IgG2A
MAB3477
100 µg
$240
None
112509
Mouse IgG2A
MAB179
500 µg
$325
None
248621
Mouse IgG2A
MAB1791
500 µg
$325
CCR7 in Human Peripheral Blood Mononuclear Cells. CCR7 was detected in human PBMCs using anti-human CCR7 monoclonal antibody (Catalog # MAB197; red). Cells were counterstained green.
CCR9
34
Human
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE CD2 CD3
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
Human
None
299812
Mouse IgG1
MAB18561
100 µg
$240
Biotin
17A2
Rat IgG2B
BAM4841
250 µg
$380
None
17A2
Rat IgG2B
MAB4841
500 µg
$325
UCHT1
Mouse IgG1
MAB100
500 µg
$325
None
Polyclonal
Goat IgG
AF2410
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2410
50 µg
$380
None
296712
Mouse IgG2B
MAB2410
100 µg
$240
None
Polyclonal
Goat IgG
AF2597
100 µg
$355
Mouse
PRICE
CD3-expressing Cells in Mouse Brain (perivascular cuffing). Cells expressing CD3 were detected in inflamed mouse brain using anti-mouse CD3 affinitypurified (Catalog # MAB4841; green). The nuclei were counterstained with DAPI (blue).
CD3e
Human
None
CD3ε in Human Peripheral Blood Mononuclear Cells (PBMCs). CD3ε was detected in human PBMCs using anti-human CD3ε monoclonal antibody (Catalog # MAB100). Cells were stained using NorthernLights-557 anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
Canine
CD4
Feline
Human
Mouse Human CD5
Mouse Feline
CD8a
372711
Mouse IgG2A
MAB25971
100 µg
$240
Polyclonal
Goat IgG
AF-379-NA
100 µg
$355
None
34930
Mouse IgG1
MAB379
500 µg
$325
Biotin
GK1.5
Rat IgG2B
BAM554
250 µg
$380
None
GK1.5
Rat IgG2B
MAB554
500 µg
$325
None
Polyclonal
Goat IgG
AF1636
100 µg
$355
Biotin
53-7.3
Rat IgG2A
BAM115
250 µg
$380
None
53-7.3
Rat IgG2A
MAB115
500 µg
$325
None
Polyclonal
Goat IgG
AF2598
100 µg
$355
Biotin
53-6.7
Rat IgG2A
BAM116
250 µg
$380
None
53-6.7
Rat IgG2A
MAB116
500 µg
$325
100 µg
$240
CD9
Mouse
None
479608
Rat IgG2A
MAB5218
CD14
Human
Biotin
Polyclonal
Sheep IgG
BAF383
50 µg
$380 $355
CD23/Fce RII
Human
None
Polyclonal
Goat IgG
AF123
100 µg
CD24
Human
None
528807
Mouse IgG2B
MAB5248
100 µg
$240
CD27/TNFRSF7
Human
Biotin
Polyclonal
Goat IgG
BAF382
50 µg
$380
CD27 Ligand/TNFSF7
Human
None
301731
Mouse IgG2B
MAB2738
100 µg
$240
CD28
Human
None
Polyclonal
Goat IgG
AF-342-PB
200 µg
$355
CD30/TNFRSF8
Mouse
None
Polyclonal
Goat IgG
AF852
100 µg
$355
CD31/PECAM-1
Human
None
Polyclonal
Sheep IgG
AF806
100 µg
$355
None
9G11
Mouse IgG1
BBA7
100 µg
$230
CD34
Rat
None
Polyclonal
Goat IgG
AF4117
100 µg
$355
CD38
Human
None
240742
Mouse IgG2A
MAB2404
500 µg
$325
Human
None
82111
Mouse IgG2B
MAB6321
500 µg
$325
Mouse
None
Polyclonal
Goat IgG
AF440
100 µg
$355
CD40/TNFRSF5
Mouse
None None
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35
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES Human
CD40 Ligand/TNFSF5 Mouse
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF617
100 µg
$355
None
40804
Mouse IgG2B
MAB617
500 µg
$325
None
Polyclonal
Goat IgG
AF1163
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1163
50 µg
$380
CD43
Human
None
290111
Mouse IgG2B
MAB2038
100 µg
$240
CD44 H
Human
None
2C5
Mouse IgG2A
BBA10
200 µg
$315
CD44 v3
Human
None
3G5
Mouse IgG2B
BBA11
200 µg
$315
CD44 v4/5
Human
None
3D2
Mouse IgG1
BBA25
200 µg
$315
CD44 v6
Human
None
2F10
Mouse IgG1
BBA13
200 µg
$315
Biotin
2D1
Mouse IgG1
BAM1430
100 µg
$295
None
2D1
Mouse IgG1
MAB1430
100 µg
$240
None
Polyclonal
Goat IgG
AF114
100 µg
$355
Biotin
30-F11
Rat IgG2B
BAM114
250 µg
$380
None
30-F11
Rat IgG2B
MAB114
500 µg
$325
None
Polyclonal
Goat IgG
AF2005
100 µg
$355
Human CD45 Mouse
CD46
Human
CD46 in Human Skin. CD46 was detected in paraffin-embedded human skin tissue sections using antihuman CD46 affinity-purified polyclonal antibody (Catalog # AF2005). Tissues were stained using antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
CD47 CD47 N-terminal IgV-like Extracellular Domain
CD48/SLAMF2
Human
None
Polyclonal
Sheep IgG
AF4670
100 µg
$355
None
Polyclonal
Goat IgG
AF1866
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1866
50 µg
$380
Human
None
394620
Mouse IgG2B
MAB36441
100 µg
$240
Mouse
None
Polyclonal
Goat IgG
AF3327
100 µg
$355
None
Polyclonal
Goat IgG
AF2009
100 µg
$355
Mouse
CD55/DAF
Human
CD58/LFA-3
Human
CD59
Human
Biotin
CD63
Mouse
None
CD68/SR-D1
Human
None
CD69
Human
Mouse CD72
CD83
36
Mouse
Biotin
Polyclonal
Goat IgG
BAF2009
50 µg
$380
None
Polyclonal
Goat IgG
AF1689
100 µg
$355
None
Polyclonal
Goat IgG
AF1987
100 µg
$355
Polyclonal
Goat IgG
BAF1987
50 µg
$380
446703
Rat IgG1
MAB5417
100 µg
$240
298807
Mouse IgG2B
MAB20401
100 µg
$240
None
Polyclonal
Goat IgG
AF2359
100 µg
$355
None
298633
Mouse IgG2A
MAB2359
100 µg
$240
None
298614
Mouse IgG2A
MAB23591
100 µg
$240
None
310116
Rat IgG1
MAB23861
100 µg
$240
None
Polyclonal
Goat IgG
AF1279
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1279
50 µg
$380
Human
None
HB15e
Mouse IgG1
MAB1774
500 µg
$325
Mouse
None
Polyclonal
Goat IgG
AF1437
100 µg
$355
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE CD109
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human
None
Polyclonal
Sheep IgG
AF4385
100 µg
$355
None
455807
Rat IgG2A
MAB4609
100 µg
$240
None
Polyclonal
Goat IgG
AF2530
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2530
50 µg
$380
None
300903
Mouse IgG1
MAB2530
100 µg
$240
CD109 in Human Squamous Cell Carcinoma. CD109 was detected in paraffin-embedded human squamous cell carcinoma sections using anti-human CD109 affinity-purified polyclonal antibody (Catalog # AF4385). Tissue was stained using the anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
CD151
CD155/PVR
CD200
CD200 R1
Mouse
Human
None
300907
Mouse IgG1
MAB25301
100 µg
$240
Human
None
Polyclonal
Goat IgG
AF2724
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF3355
100 µg
$355
Human
None
380529
Mouse IgG2B
MAB34141
100 µg
$240
None
Polyclonal
Goat IgG
AF2554
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2554
50 µg
$380 $285
Mouse
Phospho-CDC2 (Y15)
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF888
100 µg
CDC25A
Human/Mouse/Rat
None
336445
Mouse IgG2B
MAB1648
100 µg
$240
CDC25B aa 377-566
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF1649
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF2666
100 µg
$355
CDCP1 CDNF
Mouse
None
Polyclonal
Sheep IgG
AF4515
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF5097
100 µg
$355
CDNF in Human Cortex. Conserved Dopamine Neurotrophic Factor (CDNF) was detected in paraffinembedded human cortex sections using anti-human CDNF affinity-purified polyclonal antibody (Catalog # AF5097). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
CDO
Human
None
Polyclonal
Sheep IgG
AF4384
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF2429
100 µg
$355
None
Polyclonal
Goat IgG
AF3665
100 µg
$355
None
Polyclonal
Goat IgG
AF2807
100 µg
$355
None
322007
Mouse IgG2B
MAB2807
100 µg
$240
None
Polyclonal
Goat IgG
AF2244
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2244
50 µg
$380
None
283324
Mouse IgG1
MAB22441
500 µg
$325
CDO Expression in Embryonic Mouse Retina. CAMrelated/Down-regulated by Oncogenes (CDO) was detected in a cryostat tissue section of embryonic mouse retina (13 d.p.c.) using anti-mouse CDO polyclonal antibody (Catalog # AF2429) and anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown). Tissues were counterstained with hematoxylin (blue).
CDX2
Human
CDX4
Human
CEACAM-1/CD66a
Human
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37
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Sheep IgG
AF4128
100 µg
$355
None
487609
Mouse IgG2A
MAB41281
100 µg
$240
CEACAM-5/CD66e
Human
Cerberus 1
Human
None
162836
Mouse IgG2B
MAB1075
500 µg
$325
CFTR C-Terminus
Human
None
24-1
Mouse IgG2A
MAB25031
50 µg
$215
CFTR R Domain
Human
None
13-1
Mouse IgG1
MAB1660
50 µg
$215
Chitinase 3-like 1
Human
None
321806
Rat IgG2B
MAB2599
100 µg
$240
Chk2 aa 1-234
Mouse
None
235303
Rat IgG2A
MAB22851
100 µg
$240
Phospho-Chk2 (T68)
Human
None
Polyclonal
Rabbit IgG
AF1626
100 µg
$285
Human
None
316223
Rat IgG1
MAB2126
100 µg
$240
CHL-1/L1CAM-2
Mouse
None
Polyclonal
Goat IgG
AF2147
100 µg
$355
Choline Acetyltransferase/ChAT Isoform R
Human
None
Polyclonal
Goat IgG
AF3447
100 µg
$355
Chondrolectin
Human
None
396305
Mouse IgG2B
MAB2576
100 µg
$240
Chordin
Mouse
Chordin-Like 2/CHRDL2
Human
None
Polyclonal
Goat IgG
AF758
100 µg
$355
None
Polyclonal
Goat IgG
AF2448
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2448
50 µg
$380 $240
Mouse
None
328905
Rat IgG2A
MAB2520
100 µg
Chorionic Gonadotropin, a Chain (a HCG)
Human
None
381012
Mouse IgG2A
MAB4169
100 µg
$240
CIS-1
Human
None
Polyclonal
Goat IgG
AF3194
100 µg
$355
Claspin
Human
None
Polyclonal
Goat IgG
AF3310
100 µg
$355
Claudin-3
Human
None
385021
Mouse IgG2A
MAB4620
100 µg
$240
Claudin-12
Human
None
393314
Mouse IgG2A
MAB4284
100 µg
$240
Claudin-12 in Murine NS0 Cells. Claudin-12 was detected in a Claudin-12-transfected murine NS0 myeloma cell line using anti-human Claudin-12 monoclonal antibody (Catalog # MAB4284). Cells were stained using NorthernLights-557 donkey antimouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
CL-P1/COLEC12
Human
None
Polyclonal
Goat IgG
AF2690
100 µg
$355
Clusterin
Mouse
None
Polyclonal
Goat IgG
AF2747
100 µg
$355
Clusterin Isoform 1
Human
None
Polyclonal
Goat IgG
AF2937
100 µg
$355
CNP
Human
None
Polyclonal
Goat IgG
AF3127
100 µg
$355
CNP Propeptide
Human
None
347625
Rat IgG2A
MAB3739
100 µg
$240
None
Polyclonal
Total Goat IgG
AB-557-NA
1 mg
$355
None
Polyclonal
Goat IgG
AF-557-NA
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF557
None
Polyclonal
Goat IgG
AF-559-NA
Biotin
Polyclonal
Goat IgG
Polyclonal
Goat IgG
CNTF
CNTF Ra
Rat
Rat
50 µg
$380
100 µg
$355
BAF559
50 µg
$380
AF2339
100 µg
$355
CNTF Ra in Rat Brain. Ciliary Neurotrophic Factor Receptor a (CNTF Ra) was detected in a cryostat tissue section of rat brain using biotinylated anti-rat CNTF Ra polyclonal antibody (Catalog # BAF559). Tissues were stained using the anti-goat HRP-AEC Cell and Tissue Staining Kit (Catalog # CTS009; red) and counterstained with hematoxylin (blue). Antigen retrieval using Antigen retrieval-Basic Reagent is recommended (Catalog # CTS013).
Coagulation Factor III/Tissue Factor
38
Human
None
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
Coagulation Factor VII
SPECIES
Human
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF2338
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2338
50 µg
$380
None
321621
Mouse IgG1
MAB2338
100 µg
$240
Coagulation Factor VII in Human PBMCs. Coagulation Factor VII was detected in PHA-stimulated human peripheral blood mononuclear cells (PBMCs) using anti-human coagulation factor VII monoclonal antibody (Catalog # MAB2338; red). Cells were counterstained (green).
Collagen II
Mouse
None
Polyclonal
Sheep IgG
AF3615
100 µg
$355
Collectrin
Mouse
None
Polyclonal
Sheep IgG
AF4965
100 µg
$355
Complement Component C1qC aa 115-245
Human
None
Polyclonal
Goat IgG
AF3337
100 µg
$355
Complement Component C3d
Mouse
None
Polyclonal
Goat IgG
AF2655
100 µg
$355
Contactin-1
Human
None
Polyclonal
Goat IgG
AF904
100 µg
$355
Contactin-2/TAG1
Human
None
Polyclonal
Goat IgG
AF1714
100 µg
$355
None
372944
Mouse IgG2B
MAB1714
100 µg
$240
Corneodesmosin
Human
None
Polyclonal
Sheep IgG
AF5725
100 µg
$355
COUP-TF I/NR2F1
Human
None
H8132
Mouse IgG2A
PP-H8132-00
100 µg
$380
None
H8132
Mouse IgG2A
PP-H8132-10
100 µg
$380
None
H7147
Mouse IgG2A
PP-H7147-00
100 µg
$380
None
H7147
Mouse IgG2A
PP-H7147-10
100 µg
$380
COUP-TF II/NR2F2
Human
COX-2 aa 18-604
Human/Mouse Human
C-Reactive Protein/CRP
None
Polyclonal
Goat IgG
AF4198
100 µg
$355
None
Polyclonal
Sheep IgG
AF1707
100 µg
$355
Biotin
Polyclonal
Sheep IgG
BAF1707
50 µg
$380
Mouse
None
Polyclonal
Goat IgG
AF1829
100 µg
$355
Rat
None
Polyclonal
Goat IgG
AF1744
100 µg
$355
Polyclonal
Goat IgG
AF2989
100 µg
$355
C-Reactive Protein in Human Kidney Cancer. CReactive Protein (CRP) was detected in paraffinembedded human kidney cancer tissue sections using anti-human CRP affinity-purified polyclonal antibody (Catalog # AF1707). Tissues were stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
Human
None
321120
Mouse IgG1
MAB2989
100 µg
$240
CRELD1
Mouse
None
Polyclonal
Goat IgG
AF4116
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF2796
100 µg
$355 $355
CRHBP CRHR-1
Cripto
None
CREB aa 136-341
Mouse
None
Polyclonal
Goat IgG
AF3087
100 µg
Human
None
343919
Mouse IgG2A
MAB3930
100 µg
$240
None
Polyclonal
Sheep IgG
AF1538
100 µg
$355
Biotin
Polyclonal
Sheep IgG
BAF1538
50 µg
$380
None
237603
Rat IgG2B
MAB1538
150 µg
$240
MAB2771
100 µg
$240
Mouse
Cripto 1
Human
None
89646
Mouse IgG1
CRISP-3
Human
None
295203
Mouse IgG1
MAB23971
100 µg
$240
CRY1
Human/Mouse
None
Polyclonal
Goat IgG
AF3764
100 µg
$355
Cryptic
Mouse
None
221428
Rat IgG2B
MAB18401
100 µg
$240
CSL
Human
None
Polyclonal
Goat IgG
AF4079
100 µg
$355
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
39
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE CTGF/CCN2 C-Terminus
SPECIES Human
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF660
100 µg
$355
None
88430
Mouse IgG1
MAB660
500 µg
$325
CTLA-4
Human
None
Polyclonal
Goat IgG
AF-386-PB
100 µg
$355
Cubilin
Human
None
Polyclonal
Sheep IgG
AF3700
100 µg
$355
None
Polyclonal
Goat IgG
AF365
100 µg
$355
None
81513
Mouse IgG1
MAB3651
500 µg
$325
None
Polyclonal
Goat IgG
AF537
100 µg
$355
CX3CL1/Fractalkine Chemokine Domain
Human Rat
CXADR
Mouse
None
Polyclonal
Goat IgG
AF2654
100 µg
$355
CXCL1/GROa/KC/CINC-1
Rat
None
Polyclonal
Goat IgG
AF-515-NA
100 µg
$355
CXCL2/GROb/MIP-2/CINC-3
Mouse
None
Polyclonal
Goat IgG
AF-452-NA
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF452
50 µg
$380
None
Polyclonal
Goat IgG
AF254
100 µg
$355
None
33160
Mouse IgG1
MAB254
500 µg
$325
None
Polyclonal
Goat IgG
AF793
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF793
50 µg
$380
CXCL5/ENA-78
CXCL7/Thymus Chemokine-1
Human
Mouse Rat
Canine
CXCL8/IL-8
Feline
Human
CXCL8/IL-8 NorthernLights-conjugated CXCL9/MIG
Human Human Human
CXCL10/IP-10/CRG-2
CXCL12/SDF-1 CXCL13/BLC/BCA-1
Mouse
None
Polyclonal
Goat IgG
AF1116
100 µg
$355
None
Polyclonal
Goat IgG
AF1608
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1608
50 µg
$380
None
258911
Mouse IgG1
MAB1608
100 µg
$240
None
Polyclonal
Goat IgG
AF2277
100 µg
$355
None
Polyclonal
Goat IgG
AF-208-NA
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF208
50 µg
$380
None
6217
Mouse IgG1
MAB208
500 µg
$325
NL637
6217
Mouse IgG1
NL208V
500 µL
$345
None
Polyclonal
Goat IgG
AF392
100 µg
$355
None
49106
Mouse IgG1
MAB392
500 µg
$325
None
Polyclonal
Goat IgG
AF-266-NA
100 µg
$355
None
Polyclonal
Goat IgG
AF-466-NA
100 µg
$355 $380
Biotin
Polyclonal
Goat IgG
BAF466
50 µg
Human/Mouse
None
79018
Mouse IgG1
MAB350
500 µg
$325
Human
None
Polyclonal
Goat IgG
AF801
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF470
100 µg
$355
None
Polyclonal
Goat IgG
AF976
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF976
50 µg
$380 $240
CXCL16
Human
CXCR4
Feline
None
374606
Mouse IgG2A
MAB4287
100 µg
Human
None
44716
Mouse IgG2B
MAB172
500 µg
$325
Mouse
None
247506
Rat IgG2B
MAB21651
100 µg
$325
CXCR5
Human
None
51505
Mouse IgG2B
MAB190
500 µg
$325
CXCR6
Human
None
56811
Mouse IgG2B
MAB699
500 µg
$325
None
358426
Mouse IgG2A
MAB4227
100 µg
$240
None
11G8
Mouse IgG1
MAB42273
100 µg
$240
None
328303
Mouse IgG1
MAB4314
100 µg
$240
CXCR4
CXCR7/RDC-1
Human
Cyclin D1
Human
40
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Cyr61/CCN1
SPECIES Mouse
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Sheep IgG
AF4055
100 µg
$355
None
466305
Rat IgG2B
MAB4864
100 µg
$240
Cyr61 in Developing Embryonic Mouse Rib. Cyr61 was detected in cryostat sections of E13 mouse embryo using anti-mouse Cry61 affinity-purified polyclonal antibody (Catalog # AF4055). Tissues were stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
Cystatin A Cystatin B
Human
None
Polyclonal
Goat IgG
AF1407
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF1408
100 µg
$355
Mouse
None
227807
Rat IgG2A
MAB14091
500 µg
$325
Cystatin B in Mouse Cerebellum. Cystatin B was detected in a frozen section of mouse cerebellum using anti-mouse Cystatin B monoclonal antibody (Catalog # MAB14091). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).
Cystatin C
Cystatin E/M
Human
None
Polyclonal
Goat IgG
AF1196
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF1238
100 µg
$355
None
Polyclonal
Goat IgG
AF1286
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1286
50 µg
$380
None
211515
Mouse IgG2B
MAB1286
500 µg
$325
Human
Cytochrome c
Human/Equine
None
2B5F8
Mouse IgG2A
MAB898
100 µg
$240
Cytokeratin 8
Human
None
LP3K
Mouse IgG1
MAB3165
100 µg
$240
Cytokeratin 14
Human
None
LL001
Mouse IgG2A
MAB3164
100 µg
$240
Cytokeratin 19
Human
None
BA17
Mouse IgG1
MAB3506
100 µg
$240
DAN
Human
None
Polyclonal
Goat IgG
AF955
100 µg
$355
DANCE
Human
None
293904
Mouse IgG1
MAB3095
100 µg
$240
DARC
Human
None
358307
Mouse IgG2A
MAB4139
100 µg
$240
DARPP-32 aa 51-204
Mouse/Rat
375604
Rat IgG2A
MAB4230
100 µg
$240
Polyclonal
Goat IgG
AF142
100 µg
$355
DcR3/TNFRSF6B
Human
Biotin
Polyclonal
Goat IgG
BAF142
50 µg
$380
DC-SIGN
Human
None
120507
Mouse IgG2B
MAB161
500 µg
$325
DC-SIGN+DC-SIGNR
Human
None
120612
Mouse IgG2A
MAB1621
500 µg
$325
None
Polyclonal
Goat IgG
AF2378
100 µg
$355
270012
Rat IgG2A
BAM2378
100 µg
$380
DcTRAIL R1/TNFRSF23
Mouse
Biotin None
270012
Rat IgG2A
MAB2378
100 µg
$240
DDR1
Human
None
Polyclonal
Goat IgG
AF2396
100 µg
$355
DDR2
Human
None
Polyclonal
Goat IgG
AF2538
100 µg
$355
DDR2
Human
None
290814
Mouse IgG2B
MAB2538
100 µg
$240
None
290804
Mouse IgG2B
MAB25381
100 µg
$240
DEC-205/CD205
Human
None
Polyclonal
Goat IgG
AF2047
100 µg
$355
None
Polyclonal
Goat IgG
AF143
100 µg
$355
None
115402
Mouse IgG1
MAB143
500 µg
$325
None
Polyclonal
Goat IgG
AF1060
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1060
50 µg
$380
Human Decorin Mouse
None None
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
41
R&D SYSTEMS IHC/ICC ANTIBODIES SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Dectin-1/CLEC7A
MOLECULE
Human
None
259931
Mouse IgG2B
MAB1859
100 µg
$240
b-Defensin 2/BD-2
Human
None
Polyclonal
Goat IgG
AF2758
100 µg
$355
Desert Hedgehog C-Terminus
Mouse
Desert Hedgehog N-Terminus Desmin
Mouse Human
SIZE
PRICE
None
Polyclonal
Goat IgG
AF196
100 µg
$355
None
Polyclonal
Goat IgG
AF733
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF733
50 µg
$380
None
Polyclonal
Goat IgG
AF3844
100 µg
$355
None
Polyclonal
Goat IgG
AF944
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF944
50 µg
$380
None
Polyclonal
Goat IgG
AF1720
100 µg
$355
None
216519
Mouse IgG2B
MAB1720
500 µg
$325
None
Polyclonal
Goat IgG
AF3316
100 µg
$355
Desmin in Human Heart. Desmin was detected in paraffin-embedded human heart sections using antihuman desmin affinity-purified polyclonal antibody (Catalog # AF3844). Tissue was stained using the antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Desmoglein-1 Propeptide
Human
Desmoglein-3
Human
Dishevelled-1
Human
Dishevelled-1 in Human Skeletal Muscle. Dishevelled-1 was detected in a paraffin-embedded human skeletal muscle section using anti-human dishevelled-1 affinity-purified polyclonal antibody (Catalog # AF3316). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Dishevelled-3
Human
None
Polyclonal
Sheep IgG
AF3754
100 µg
$355
None
Polyclonal
Goat IgG
AF1765
100 µg
$355
259631
Rat IgG2B
BAM1765
100 µg
$380
Dkk-1
Mouse
Biotin None
259631
Rat IgG2B
MAB1765
100 µg
$240
Dkk-2
Mouse
None
Polyclonal
Goat IgG
AF2435
100 µg
$355
Dkk-3
Human
None
Polyclonal
Goat IgG
AF1118
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1118
50 µg
$380
DLEC/CLEC4C/BDCA2
Human
None
Polyclonal
Goat IgG
AF1376
100 µg
$355
Human
None
251123
Mouse IgG2B
MAB18181
100 µg
$240
Mouse/Rat
None
Polyclonal
Sheep IgG
AF5026
100 µg
$355
Rat
None
Polyclonal
Sheep IgG
AF3970
100 µg
$355
422208
Mouse IgG2B
MAB4207
100 µg
$240
DLL1
DLL1 Expression in Mouse Embyronic Stomach. Delta-like 1 (DLL1) was detected in a cryostat tissue section of mouse E13.5 embryonic stomach using anti-rat DLL1 affinity-purified polyclonal antibody (Catalog # AF3970). Tissues were stained using NorthernLights-557-conjugated anti-sheep secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
DMC/VCC-1
42
Human
None
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE DMP-1
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Mouse
None
Polyclonal
Sheep IgG
AF4386
100 µg
$355
Polyclonal
Goat IgG
AF3646
100 µg
$355
DMP-1 in Developing Mouse Teeth. Dentin Matrix Protein 1 (DMP-1) was detected in a cryostat section of developing embryonic (E15.5) mouse teeth using anti-mouse DMP-1 affinity-purified polyclonal antibody (Catalog # AF4386). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
Human DNER
DOK4
None Biotin
Polyclonal
Goat IgG
BAF3646
50 µg
$380
None
Polyclonal
Goat IgG
AF2254
100 µg
$355
Biotin
298306
Rat IgG1
BAM2254
100 µg
$380
Mouse
None
298306
Rat IgG1
MAB2254
100 µg
$240
Human
None
Polyclonal
Sheep IgG
AF4826
100 µg
$355
Polyclonal
Goat IgG
AF3564
100 µg
$355
Mouse
DOK4 in Human Kidney. Downstream of kinase 4 (DOK4) was detected in a paraffin-embedded section of human kidney using anti-human DOK4 affinitypurified polyclonal antibody (Catalog # AF4826). Tissues were stained using anti-sheep HRP-DAB detection reagents (brown) and counterstained with hematoxylin (blue).
Dopa Decarboxylase/DDC
Human
None
Dopamine Transporter C-Terminus
Human/Mouse/Primate
None
Polyclonal
Rabbit IgG
PPS068
100 µL
$355
Dopamine Transporter Extracellular Loop 2
Human/Primate
None
Polyclonal
Rabbit IgG
PPS069
100 µL
$355
DPP6
Human
None
Polyclonal
Goat IgG
AF2360
100 µg
$355
DPPA4
DPPA5/ESG1
Human
None
Polyclonal
Sheep IgG
AF3674
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF3730
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF3125
100 µg
$355
None
Polyclonal
Goat IgG
AF3984
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF3984
50 µg
$380
None
Polyclonal
Goat IgG
AF1180
100 µg
$355
None
Polyclonal
Goat IgG
AF954
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF954
50 µg
$380
None
Polyclonal
Goat IgG
AF2437
100 µg
$355
None
Polyclonal
Goat IgG
AF3666
100 µg
$355
None
399212
Mouse IgG2B
MAB36661
100 µg
$240
329707
Mouse IgG1
MAB3110
100 µg
$240
Mouse Human
DPPIV/CD26
Mouse
DR3/TNFRSF25
Mouse
DSCAM Long Isoform
Human
DSCAM in Human A172 Cells. Down Syndrome Cell Adhesion Molecule (DSCAM) was detected in human A172 glioblastoma cell line using anti-human DSCAM affinity-purified polyclonal antibody (Catalog # AF3666). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
DSPG3
Human
None
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43
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Human
None
96201
Mouse IgG1
MAB859
500 µg
$325
None
Polyclonal
Goat IgG
AF759
100 µg
$355
Biotin
109646
Rat IgG1
BAM759
100 µg
$380
None
109646
Rat IgG1
MAB759
500 µg
$325
Phospho-Dynamin (S774)
Human/Mouse/Rat/Bovine/ Canine/Chicken/Primate
None
Polyclonal
Sheep IgG
PPS004
100 µL
$355
DYRK1A aa 438-571
Human/Rat
None
Polyclonal
Sheep IgG
AF5407
100 µg
$355 $355
Dtk
Mouse
SIZE
PRICE
E2F-1
Human
None
Polyclonal
Sheep IgG
AF4825
100 µg
EAR2/NR2F6
Human
None
N2025
Mouse IgG2A
PP-N2025-00
100 µg
$380
ECE-1
Human
None
Polyclonal
Goat IgG
AF1784
100 µg
$355
ECE-2
Human
None
233914
Rat IgG2A
MAB16451
100 µg
$240
ECM-1
Human
None
Polyclonal
Sheep IgG
AF3937
100 µg
$355
None
Polyclonal
Goat IgG
AF236
100 µg
$355
None
10825
Mouse IgG1
MAB236
500 µg
$325
None
10827
Mouse IgG1
MAB636
500 µg
$325
None
Polyclonal
Goat IgG
AF2028
100 µg
$355
ECM-1 in Human Colon Cancer. Extracellular matrix protein-1 (ECM-1) was detected in paraffin-embedded sections of human colon cancer using antihuman ECM-1 affinity-purified polyclonal antibody (Catalog # AF3937). Tissue was stained using antisheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
Human EGF Mouse
EGF R/ErbB1
Mouse
Phospho-EGF R/ErbB1 (Y1173)
Human
Phospho-EGF R/ErbB1 (Y845)
Human
EGR1
Human
None
463004
Rat IgG2A
MAB5256
100 µg
$240
None
Polyclonal
Goat IgG
AF1280
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1280
50 µg
$380
None
Polyclonal
Rabbit IgG
AF1095
50 µg
$285
None
Polyclonal
Rabbit IgG
AF3394
50 µg
$285
None
Polyclonal
Goat IgG
AF2818
100 µg
$355
None
406913
Rat IgG2A
MAB2818
100 µg
$240
EGR1 in Human Prostate Cancer. Early growth response 1 (EGR1) was detected in a paraffin-embedded human prostate cancer tissue section using antihuman EGR1 monoclonal antibody (Catalog # MAB2818). Tissues were stained using the anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).
eIF4E
Human/Mouse/Rat
None
299910
Mouse IgG2B
MAB3228
100 µg
$240
ELF3 aa 375-552
Human/Mouse
None
Polyclonal
Goat IgG
AF5787
100 µg
$355
EMAP-II
Human
None
Polyclonal
Goat IgG
AF1910
100 µg
$355
EMAP-II in Human Colon Cancer. Endothelial Monocyte-activation Polypeptide II (EMAP-II) was detected in a paraffin-embedded human colon cancer section using anti-human EMAP-II affinity-purified polyclonal antibody (Catalog # AF1910). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
44
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
EMMPRIN
MOLECULE
Human
None
Polyclonal
Goat IgG
AF972
100 µg
$355
EMR1
Mouse
None
521204
Rat IgG2A
MAB5580
100 µg
$240
None
Polyclonal
Goat IgG
AF1097
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1097
50 µg
$380
None
Polyclonal
Goat IgG
AF1320
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1320
50 µg
$380
None
209701
Rat IgG2A
MAB1320
100 µg
$240
None
Polyclonal
Goat IgG
AF1524
100 µg
$355
None
211816
Mouse IgG2A
MAB1524
100 µg
$240
None
Polyclonal
Goat IgG
AF3534
100 µg
$355
Polyclonal
Goat IgG
AF4666
100 µg
$355
Human Endoglin/CD105 Mouse
Endoglycan/PODLX2
Human Mouse
Endoglycan in Mouse Testis. Endoglycan was detected in a cryostat section of mouse testis using antimouse Endoglycan affinity-purified polyclonal antibody (Catalog # AF3534). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Endomucin
Mouse
Endorepellin/Perlecan
Human
Endostatin
Human Mouse
EDNRB/Endothelin R Type B
None None
Polyclonal
Goat IgG
AF2364
100 µg
$355
None
Polyclonal
Goat IgG
AF1098
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1098
50 µg
$380
None
Polyclonal
Goat IgG
AF570
100 µg
$355
Human
None
Polyclonal
Sheep IgG
AF4496
100 µg
$355
Endothelin-1 C-Terminus, aa 53-73 (EDN1) and 15-22 (Mature)
Human
None
3G10
Rat IgG2B
MAB3440
100 µg
$240
Engrailed-2
Human
None
279122
Mouse IgG2B
MAB2600
100 µg
$240
Enolase 2
Human/Mouse
None
Polyclonal
Sheep IgG
AF5169
100 µg
$355
ENPP-7/Alk-SMase
Mouse
EN-RAGE/S100A12
Human
EpCAM/TROP-1
Human
Human EPCR Mouse
None
Polyclonal
Sheep IgG
AF5160
100 µg
$355
None
Polyclonal
Goat IgG
AF1052
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1052
50 µg
$380
None
158210
Mouse IgG2A
MAB960
500 µg
$325
None
Polyclonal
Goat IgG
AF2245
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2245
50 µg
$380
None
304519
Mouse IgG2B
MAB22451
100 µg
$240
None
Polyclonal
Goat IgG
AF2749
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2749
50 µg
$380
EPCR in Normal Human Skin. Endothelial Protein C Receptor (EPCR) was detected in a paraffin-embedded human skin section using anti-human EPCR monoclonal antibody (Catalog # MAB22451). The tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
Human EphA1
Mouse
None
Polyclonal
Goat IgG
AF638
100 µg
$355
None
Polyclonal
Goat IgG
AF3034
100 µg
$355
None
404517
Rat IgG2A
MAB30341
100 µg
$240
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
45
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES Human
EphA2 Mouse EphA3
Mouse
EphA4
Mouse
EphA5
Rat
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF3035
100 µg
$355
None
371805
Mouse IgG2A
MAB3035
100 µg
$240
None
Polyclonal
Goat IgG
AF639
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF639
50 µg
$380
None
Polyclonal
Goat IgG
AF640
100 µg
$355
None
Polyclonal
Goat IgG
AF641
100 µg
$355
None
Polyclonal
Goat IgG
AF541
100 µg
$355
None
86731
Mouse IgG1
MAB541
500 µg
$325
None
Polyclonal
Goat IgG
AF542
100 µg
$355
None
88512
Mouse IgG1
MAB5421
100 µg
$240
None
Polyclonal
Goat IgG
AF467
100 µg
$355
None
512012
Rat IgG2A
MAB467
100 µg
$240
None
512001
Rat IgG2A
MAB4671
100 µg
$240
Polyclonal
Goat IgG
AF432
100 µg
$355
EphA5 in Human U-118 MG Cells. EphA5 was detected in human U-118 MG glioblastoma cell line using anti-rat EphA5 monoclonal antibody (Catalog # MAB541). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
EphB1
EphB2
Rat
Mouse
EphB2 in Embryonic Mouse Brain. EphB2 was detected in a frozen section of E15 mouse brain using anti-mouse EphB2 affinity-purified polyclonal antibody (Catalog # AF467). Tissues were stained using NorthernLights-557 secondary antibody (Catalog # NL001; red) and counterstained (green).
EphB3
Mouse Human
EphB4
Ephrin-A2
Mouse Mouse
None None
Polyclonal
Goat IgG
AF3038
100 µg
$355
None
Polyclonal
Goat IgG
AF446
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF446
50 µg
$380
None
Polyclonal
Goat IgG
AF603
100 µg
$355
Ephrin-A2 in Rat Embryonic Neurons. Ephrin-A2 was detected in rat embryonic hippocampal neurons using anti-mouse Ephrin-A2 affinity-purified polyclonal antibody (Catalog # AF603; green). Astrocytes were stained using an anti-GFAP antibody (red).
Ephrin-A5
Human
None
Polyclonal
Goat IgG
AF3743
100 µg
$355
Ephrin-B1
Mouse
None
Polyclonal
Goat IgG
AF473
100 µg
$355
Ephrin-B2
Mouse
None
Polyclonal
Goat IgG
AF496
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF496
50 µg
$380
Ephrin-B3
Human
None
Polyclonal
Goat IgG
AF395
100 µg
$355
Epigen
Mouse
None
Polyclonal
Goat IgG
AF1127
100 µg
$355
46
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Epimorphin/Syntaxin 2
SPECIES Mouse
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF2568
100 µg
$355
None
338930
Rat IgG2A
MAB2568
100 µg
$240
Epimorphin/Syntaxin 2 in Mouse Eye. Epimorphin was detected in frozen embryonic mouse eye cup sections using anti-mouse Epimorphin monoclonal antibody (Catalog # MAB2568). Tissue was stained using the anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).
Epiregulin
ErbB2/Her2
Human
Human
None
Polyclonal
Goat IgG
AF1195
100 µg
$355
None
Polyclonal
Goat IgG
AF1129
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1129
50 µg
$380
None
191924
Mouse IgG2B
MAB1129
500 µg
$325
None
416711
Mouse IgG2B
MAB11291
100 µg
$240
Polyclonal
Rabbit IgG
AF4438
100 µg
$285
Phospho-ErbB2 in Human Breast Cancer. Phospho-ErbB2 was detected in paraffin-embedded human breast cancer tissue sections using anti-human phospho-ErbB2 affinity-purified polyclonal antibody (Catalog # AF1768). Tissues were stained using antirabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue)
Phospho-ErbB2/Her2 (Y1196)
Human
Phospho-ErbB2/Her2 (Y1248)
Human
ErbB3/Her3
ERK1/ERK2
Human
None None
Polyclonal
Rabbit IgG
AF1768
50 µg
$285
None
66219
Mouse IgG1
MAB348
500 µg
$325
None
526806
Mouse IgG2B
MAB3483
100 µg
$240
Mouse
None
Polyclonal
Sheep IgG
AF4518
100 µg
$355
Human/Mouse/Rat
None
216703
Mouse IgG1
MAB1576
100 µg
$240
Polyclonal
Rabbit IgG
AF1018
100 µg
$285
50 µg
$285
Phosphorylated ERK1/ERK2 Expression in Rat Brain Cortex. Phosphorylated ERK1/ERK2 was detected in cryostat tissue sections of rat brain cortex using anti-human/mouse/rat phospho-ERK1/ERK2 affinity-purified polyclonal antibody (Catalog # AF1018). Tissue was stained using the anti-rabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue).
Phospho-ERK1/ERK2 (ERK1 T202/Y204, ERK2 T185/Y187)
Human/Mouse/Rat
None
ERK1
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF1575
ERK1 aa 1-28
Human
None
250603
Mouse IgG2A
MAB1940
100 µg
$240
None
Polyclonal
Rabbit IgG
AF1230
100 µg
$285
None
Polyclonal
Goat IgG
AF12301
100 µg
$355 $240
ERK2
Human/Mouse/Rat
None
191801
Mouse IgG2A
MAB1230
100 µg
ERK3 aa 344-721
Human
None
335102
Mouse IgG2B
MAB3196
100 µg
$240
ERK4 aa 1-126
Human/Mouse/Rat
None
355810
Rat IgG2A
MAB3914
100 µg
$240
ERK5/BMK1 aa 1-432
Human/Mouse
None
Polyclonal
Goat IgG
AF2848
100 µg
$355
None
H5844
Mouse IgG2A
PP-H5844-00
100 µg
$380
None
H5844
Mouse IgG2A
PP-H5844-10
100 µg
$380
ERRa/NR3B1
Human
ERRb/NR3B2
Human
None
H6705
Mouse IgG2A
PP-H6705-00
100 µg
$380
ERRg/NR3B3
Human
None
H6812
Mouse IgG2A
PP-H6812-00
100 µg
$380
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
47
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Erythropoietin Erythropoietin R
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
Human
None
Polyclonal
Total Rabbit IgG
AB-286-NA
1 mg
$355
None
Polyclonal
Goat IgG
AF1390
100 µg
$355
Mouse
Human ESAM Mouse
PRICE
Biotin
Polyclonal
Goat IgG
BAF1390
50 µg
$380
None
Polyclonal
Goat IgG
AF2688
100 µg
$355
None
408519
Mouse IgG2B
MAB4204
100 µg
$240
None
408522
Mouse IgG2B
MAB42041
100 µg
$240
None
Polyclonal
Goat IgG
AF2827
100 µg
$355
None
340236
Rat IgG2A
MAB28271
100 µg
$240
ESAM in Human Kidney. Endothelial Cell Adhesion Molecule (ESAM) was detected in paraffin-embedded human kidney sections using anti-human ESAM monoclonal antibody (Catalog # MAB42041). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown). Tissue was counterstained with hematoxylin (blue).
ESGP
Mouse
ERa/NR3A1
Human
EZH2
Human/Mouse Human
FABP1 Rat
FABP2
FABP3
Polyclonal
Sheep IgG
AF4580
100 µg
$355
Polyclonal
Sheep IgG
AF5715
100 µg
$355
None
H4624
Mouse IgG2A
PP-H4624-00
100 µg
$380
None
Polyclonal
Goat IgG
AF4767
100 µg
$355
None
Polyclonal
Goat IgG
AF2964
100 µg
$355
None
328607
Mouse IgG2A
MAB2964
100 µg
$240
None
Polyclonal
Goat IgG
AF1565
100 µg
$355
None
220119
Mouse IgG2B
MAB1565
100 µg
$240
Human
None
Polyclonal
Goat IgG
AF3078
100 µg
$355
Rat
None
Polyclonal
Goat IgG
AF1486
100 µg
$355
None
Polyclonal
Sheep IgG
AF1678
100 µg
$355
Biotin
Polyclonal
Sheep IgG
BAF1678
50 µg
$380
None
242305
Rat IgG2A
MAB1678
100 µg
$240
Human
Human FABP4
None None
Mouse
None
Polyclonal
Goat IgG
AF3150
100 µg
$355
None
Polyclonal
Goat IgG
AF1443
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1443
50 µg
$380
None
258417
Rat IgG2A
MAB1443
100 µg
$240
None
Polyclonal
Goat IgG
AF3077
100 µg
$355
None
311215
Rat IgG2A
MAB3077
100 µg
$240
FABP4 in Mouse Mesenchymal Stem Cells. Fatty Acid Binding Protein 4 (FABP4) was detected in mouse mesenchymal stem cells using anti-mouse FABP4 affinity-purified polyclonal antibody (Catalog # AF1443). Cells were stained using anti-goat NorthernLights-557 (Catalog # NL001; red) and counterstained with DAPI (blue).
FABP5FABP5
Human Mouse
None
Polyclonal
Goat IgG
AF1476
100 µg
$355
FABP5
Mouse
Biotin
Polyclonal
Goat IgG
BAF1476
50 µg
$380
FABP6
Human
None
Polyclonal
Goat IgG
AF3880
100 µg
$355
FABP7
Human
FABP9
Mouse
48
Biotin
Polyclonal
Goat IgG
BAF3880
50 µg
$380
None
Polyclonal
Goat IgG
AF3166
100 µg
$355
None
Polyclonal
Goat IgG
AF2750
100 µg
$355
None
324926
Rat IgG2A
MAB2750
100 µg
$240
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE FAK aa 213-412
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Human
None
495919
Mouse IgG2B
MAB4467
100 µg
SIZE
PRICE $240
Human/Mouse/Rat
None
Polyclonal
Sheep IgG
AF4467
100 µg
$355
FAK in Human Hippocampus. Focal adhesion kinase 1 (FAK) was detected in paraffin-embedded human brain hippocampus sections using anti-human/ mouse/rat FAK affinity-purified polyclonal antibody (Catalog # AF4467). The tissue was stained using the anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
Fibroblast Activation Protein a/FAP
Human
None
Polyclonal
Sheep IgG
AF3715
100 µg
$355
None
Polyclonal
Goat IgG
AF2267
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2267
50 µg
$380
Human
None
DX2
Mouse IgG1
MAB142
500 µg
$325
Mouse
None
Polyclonal
Goat IgG
AF435
100 µg
$355
Rat
None
Polyclonal
Goat IgG
AF2159
100 µg
$355
Feline Fas/TNFRSF6/CD95
Human Fas Ligand/TNFSF6
Polyclonal
Goat IgG
AF126
100 µg
$355
Polyclonal
Goat IgG
AF1858
100 µg
$355
None
252029
Mouse IgG2B
MAB1858
500 µg
$325 $240
FATP1
Human
None
308420
Mouse IgG2B
MAB3304
100 µg
Fcg RI/CD64
Human
None
10.1
Mouse IgG1
MAB1257
500 µg
$325
Fcg RII/RIII (CD32/CD16)
Mouse
None
Polyclonal
Goat IgG
AF1460
100 µg
$355
Fcg RIIB/CD32b
Human
None
Polyclonal
Goat IgG
AF1330
100 µg
$355
Fcg RIIIA/B (CD16)
Mouse
FCRL4/FcRH4
Human
Biotin
Polyclonal
Goat IgG
BAF2426
50 µg
$380
Fetuin A/AHSG
Human
None
Polyclonal
Goat IgG
AF1184
100 µg
$355
FGF acidic
Human
None
Polyclonal
Goat IgG
AF232
100 µg
$355
None
Polyclonal
Total Goat IgG
AB-233-NA
1 mg
$355
FGF basic
Human
None
Polyclonal
Goat IgG
AF-233-NA
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF233
50 µg
$380
None
Polyclonal
Goat IgG
AF1206
100 µg
$355
None
254625
Mouse IgG2A
MAB1206
500 µg
$325
None
Polyclonal
Goat IgG
AF235
100 µg
$355
FGF-3
FGF-4
FGF-5
KGF/FGF-7
Rat
None None
Human
Human
Human Canine
Biotin
Polyclonal
Goat IgG
BAF1330
50 µg
$380
None
Polyclonal
Goat IgG
AF1960
100 µg
$355
None
Polyclonal
Goat IgG
AF2426
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF235
50 µg
$380
None
Polyclonal
Goat IgG
AF-237-NA
100 µg
$355
None
221903
Mouse IgG1
MAB2371
500 µg
$325
None
299026
Mouse IgG1
MAB1957
100 µg
$240 $315
Human
None
Polyclonal
Goat IgG
AF-251-NA
250 µg
FGF-8
Human/Mouse
None
47109
Mouse IgG1
MAB323
500 µg
$325
FGF-8b Isoform
Mouse
None
Polyclonal
Goat IgG
AF-423-NA
100 µg
$355
FGF-9
Human
None
Polyclonal
Goat IgG
AF-273-NA
100 µg
$355
FGF-10
Human
None
Polyclonal
Goat IgG
AF345
100 µg
$355
FGF-17
Human
None
121630
Mouse IgG2B
MAB319
500 µg
$325
FGF-19
Human
None
117601
Mouse IgG1
MAB969
500 µg
$325
None
Polyclonal
Goat IgG
AF2547
100 µg
$355
None
272317
Rat IgG2A
MAB2547
100 µg
$240
FGF-20
Human
FGF-21
Human
None
461804
Mouse IgG2B
MAB25372
100 µg
$240
FGF-23
Mouse
None
283507
Rat IgG2A
MAB26291
100 µg
$240
FGF-BP
Rat
None
Polyclonal
Goat IgG
AF1413
100 µg
$355
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
49
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
FGF R2
Human
None
98739
Mouse IgG1
FGF R3 (IIIc)
Human
None
136312
Mouse IgG1
Human
None
240929
None Biotin
FGF R4
Mouse
SIZE
PRICE
MAB6843
500 µg
$325
MAB7662
500 µg
$325
Rat IgG2A
MAB6852
100 µg
$240
Polyclonal
Goat IgG
AF2265
100 µg
$355
Polyclonal
Goat IgG
BAF2265
50 µg
$380
AF1899
100 µg
$355
FGF R5/FGFRL1
Mouse
None
Polyclonal
Goat IgG
Fgr
Human
None
349331
Mouse IgG2A
MAB32071
100 µg
$240
Fgr aa 1-82
Human/Rat
None
Polyclonal
Goat IgG
AF3207
100 µg
$355
None
Polyclonal
Sheep IgG
AF1918
100 µg
$355
None
P1H11
Mouse IgG1
MAB1918
100 µg
$240
None
P1F11
Mouse IgM
MAB19181
100 µg
$240
Fibronectin
Human
Fibronectin in Human WS-1 Cells. Fibronectin was detected in WS-1 human dermal fibroblasts using anti-human Fibronectin monoclonal antibody (Catalog # MAB19181). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
FKBP38 aa 1-326 FKBP51
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF3580
100 µg
$355
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF4094
100 µg
$355
FKBP51 in Human Prostate. FK506 Binding Protein, 51 kDa molecular weight (FKBP51), was detected in paraffin-embedded human prostate sections using anti-human/mouse/rat FKBP51 affinity-purified polyclonal antibody (Catalog # AF4094). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
FKBP52
Human
None
425123
Rat IgG1
MAB4095
100 µg
$240
None
Polyclonal
Goat IgG
AF1255
100 µg
$355 $380
Follistatin-related Gene Protein/FLRG
Mouse
Biotin
Polyclonal
Goat IgG
BAF1255
50 µg
Flt-3/Flk-2
Mouse
None
113308
Rat IgG2A
MAB7681
100 µg
$240
Phospho-Flt-3/Flk-2 (Y591)
Human
None
Polyclonal
Rabbit IgG
AF368
50 µg
$285
Flt-3 Ligand
Feline
Follistatin
Human
None
Polyclonal
Goat IgG
AF2467
100 µg
$355
None
Polyclonal
Goat IgG
AF669
100 µg
$355
None
85918
Mouse IgG2A
MAB669
500 µg
$325
Human
None
Polyclonal
Goat IgG
AF1694
100 µg
$355
Mouse/Rat
None
228208
Rat IgG2B
MAB17381
100 µg
$240
FOLR1
Human
None
548908
Mouse IgG1
MAB5646
100 µg
$240
FosB/G0S3
Human
Follistatin-like 1/FSL1
FoxH1
FoxJ1
50
Human
Human
None
Polyclonal
Goat IgG
AF2214
100 µg
$355
None
Polyclonal
Sheep IgG
AF4248
100 µg
$355
Biotin
Polyclonal
Sheep IgG
BAF4248
50 µg
$380
None
Polyclonal
Goat IgG
AF3619
100 µg
$355
None
407003
Rat IgG2B
MAB3619
100 µg
$240
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE FoxM1
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human
None
Polyclonal
Sheep IgG
AF3975
100 µg
$355
FoxM1 in Human U2OS Cells. FoxM1 was detected in human osteosarcoma U2OS cells using antihuman FoxM1 affinity-purified polyclonal antibody (Catalog # AF3975). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained (green).
FoxP2 FoxP3
Human/Mouse
None
Polyclonal
Sheep IgG
AF5647
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF3240
100 µg
$355
FoxP3 in Human Tonsil. FoxP3 was detected in paraffin-embedded human tonsil sections using antihuman FoxP3 affinity-purified polyclonal antibody (Catalog # AF3240). Tissue was stained using antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Human/Mouse Frizzled-1
Mouse
Biotin
162531
Rat IgG2A
BAM11201
100 µg
$240
None
Polyclonal
Goat IgG
AF1120
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1120
50 µg
$380
None
169310
Rat IgG2A
MAB1001-050
50 µg
$150
None
169310
Rat IgG2A
MAB1001-100
100 µg
$240
None
Polyclonal
Goat IgG
AF1001
100 µg
$355
Biotin
169310
Rat IgG2A
BAM1001
100 µg
$295
Biotin
145901
Rat IgG2A
BAM194
100 µg
$240
None
145901
Rat IgG2A
MAB194-050
50 µg
$150
None
145901
Rat IgG2A
MAB194-100
100 µg
$240
None
Polyclonal
Goat IgG
AF194
100 µg
$355
Frizzled-1 Expression in Mouse Intestine. Friz zled-1 was detected in a cryostat tissue section of embryonic mouse intestine (E13) using anti-mouse Frizzled-1 affinity-purified polyclonal antibody (Catalog # AF1120). Tissues were stained using the antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Human/Mouse Frizzled-3 Mouse
Frizzled-4
Human/Mouse
Mouse Frizzled-6
Frizzled-7
Mouse Human/Mouse
None
Polyclonal
Goat IgG
AF1526
100 µg
$355
None
151143
Rat IgG2A
MAB1981-050
50 µg
$150
None
151143
Rat IgG2A
MAB1981-100
100 µg
$240
Mouse
None
Polyclonal
Goat IgG
AF198
100 µg
$355
Frizzled-8
Mouse
None
Polyclonal
Goat IgG
AF112
100 µg
$355
Frizzled-9
Mouse
None
Polyclonal
Goat IgG
AF2440
100 µg
$355
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
51
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Frk aa 1-107
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Human
None
393812
Rat IgG1
MAB3766
100 µg
SIZE
PRICE $240
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF3766
100 µg
$355
Frk in Human Breast Cancer. Fyn-related kinase (Frk) was detected in paraffin-embedded human breast cancer sections using anti-human Frk monoclonal antibody (Catalog # MAB3766). Tissue was subjected to antigen retrieval using Antigen Retrieval Kit (Catalog # CTS013; brown). Tissue was stained using anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).
FRS2
Human
None
Polyclonal
Sheep IgG
AF4069
100 µg
$355
Fucosyltransferase 8/FUT8
Human
None
Polyclonal
Sheep IgG
AF5768
100 µg
$355
Furin
Human
None
222728
Mouse IgG1
MAB15031
500 µg
$325
FXR/NR1H4
Human
None
A9033A
Mouse IgG2A
PP-A9033A-00
100 µg
$380
Fyn
Human
None
Polyclonal
Goat IgG
AF3574
100 µg
$355
G9a/EHMT2
Human/Mouse
None
A8620A
Mouse IgG2A
PP-A8620A-00
100 µg
$380
GABAA R d N-Terminus
Mouse/Rat
None
Polyclonal
Rabbit IgG
PPS090
100 µL
$355
GABAB R2 N-Terminus
Rat
None
Polyclonal
Goat IgG
AF1188
100 µg
$355
GAD1/GAD67 aa 2-97
Human
None
Polyclonal
Goat IgG
AF2086
100 µg
$355
None
252910
Mouse IgG1
MAB2086
100 µg
$240
GAD2/GAD65 aa 2-150
Human
None
Polyclonal
Goat IgG
AF2247
100 µg
$355
Human
Galectin-1
Mouse
Human Galectin-3 Mouse
Galectin-4
None
Polyclonal
Goat IgG
AF1152
100 µg
$355
None
Polyclonal
Goat IgG
AF1245
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1245
50 µg
$380
Biotin
201002
Rat IgG2B
BAM12451
250 µg
$380
None
201002
Rat IgG2B
MAB12451
500 µg
$325
None
Polyclonal
Goat IgG
AF1154
100 µg
$355
None
194804
Mouse IgG2B
MAB1154
500 µg
$325
None
Polyclonal
Goat IgG
AF1197
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1197
50 µg
$380
Human
None
Polyclonal
Goat IgG
AF1227
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF2128
100 µg
$355
None
Polyclonal
Goat IgG
AF1339
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1339
50 µg
$380
None
Polyclonal
Goat IgG
AF1304
100 µg
$355
None
212923
Rat IgG2A
MAB1304
500 µg
$325
None
Polyclonal
Goat IgG
AF1305
100 µg
$355
None
Polyclonal
Goat IgG
AF2045
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2045
50 µg
$380
Human Galectin-7 Mouse Galectin-8
Human
Galectin-9
Human
Galectin-9 Short Isoform
Mouse
None
Polyclonal
Goat IgG
AF3535
100 µg
$355
Gas1
Mouse
None
Polyclonal
Goat IgG
AF2644
100 µg
$355
Gas6
Human
None
Polyclonal
Goat IgG
AF885
100 µg
$355
GASP-1/WFIKKNRP
Human
None
288518
Mouse IgG2B
MAB20701
100 µg
$240
GASP-2/WFIKKN
Human
None
289802
Mouse IgG2B
MAB21363
100 µg
$240
GATA-1
Human
None
234732
Rat IgG2B
MAB1779
100 µg
$240
GATA-2
Human
None
Polyclonal
Goat IgG
AF2046
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2046
50 µg
$380
GATA-3
Human
None
291106
Mouse IgG1
MAB26051
100 µg
$240
GATA-3 aa 135-258
Human
None
Polyclonal
Goat IgG
AF2605
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2605
50 µg
$380
52
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE GATA-4
SPECIES Human
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF2606
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2606
50 µg
$380
GATA-4 in Differentiated Human Embryonic Stem Cells. GATA-4 was detected in differentiated human Embryonic Stem cells using anti-human GATA-4 polyclonal antibody (Catalog # AF2606; green). The cells were counterstained with DAPI (blue). Courtesy of Dr. Frank Solder from the National Institute of Neurological Disorders and Stroke & Stem Cell Unit at NIH.
GATA-5
Human
None
Polyclonal
Goat IgG
AF2170
100 µg
$355
None
Polyclonal
Goat IgG
AF1700
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1700
50 µg
$380
GATA-6
Human
None
222228
Mouse IgG1
MAB1700
100 µg
$240
GCNF/NR6A1
Human
None
Polyclonal
Goat IgG
AF3180
100 µg
$355
GDF-3
Mouse
None
Polyclonal
Goat IgG
AF958
100 µg
$355
None
Polyclonal
Goat IgG
AF853
100 µg
$355
GDF-5
Mouse
Biotin
Polyclonal
Goat IgG
BAF853
50 µg
$380
Biotin
90509
Rat IgG2A
BAM853
50 µg
$380
None
Polyclonal
Goat IgG
AF788
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF788
50 µg
$380
None
Polyclonal
Goat IgG
AF739
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF739
50 µg
$380
GDF-8/Myostatin
Mouse
GDF-9
Mouse
GDNF
Human/Rat
GFAP
Human
None
Polyclonal
Goat IgG
AF-212-NA
100 µg
$355
None
Polyclonal
Sheep IgG
AF2594
100 µg
$355
Biotin
Polyclonal
Sheep IgG
BAF2594
50 µg
$380
None
273807
Mouse IgG1
MAB2594
100 µg
$240
Polyclonal
Goat IgG
AF3540
100 µg
$355
GFAP in Rat Astrocytes. Glial fibrillary acidic protein (GFAP) was detected in astrocytes in differentia ting cultures of rat cortical stem cells using antihuman GFAP affinity-purified polyclonal antibody (Catalog # AF2594). The cells were stained using NorthernLights-557 anti-sheep secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
GFI-1
Human Human
GFRa-1/GDNF Ra-1
Rat
None None
Polyclonal
Goat IgG
AF714
100 µg
$355
None
Polyclonal
Goat IgG
AF560
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF560
50 µg
$380
None
81401
Mouse IgG2B
MAB560
500 µg
$325
GFRa-1 and GDNF Expression in Rat Dorsal Root Ganglia. GFRa-1 and GDNF were detected in rat dorsal root ganglia using biotinylated anti-rat GFRa-1 affinity-purified polyclonal antibody (Catalog # BAF560; red) and anti-human GDNF affinitypurified polyclonal antibody (Catalog # AF-212-NA; green).
GFRa-2/GDNF Ra-2
Human
None
Polyclonal
Goat IgG
AF613
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF429
100 µg
$355
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
53
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
GFRa-3/GDNF Ra-3
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Human
None
Polyclonal
Goat IgG
AF670
100 µg
$355
None
Polyclonal
Goat IgG
AF2645
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2645
50 µg
$380
Mouse
SIZE
PRICE
GFRa-3 in Mouse Embryonic Dorsal Root Ganglion (DRG). Detection of Glial cell line-derived Factor Receptor alpha 3 (GFRa-3) in a cryostat section of E15 DRG mouse using anti-mouse GFRa-3 affinity-purified polyclonal antibody (Catalog # AF2645). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (brown; Catalog # CTS008 and counterstained with hematoxylin (blue).
None
Polyclonal
Goat IgG
AF1677
100 µg
$355
GFRa-4/GDNF Ra-4
Mouse
Biotin
Polyclonal
Goat IgG
BAF1677
50 µg
$380
None
171217
Rat IgG2A
MAB1677
500 µg
$325
GITR/TNFRSF18
Human
None
Polyclonal
Goat IgG
AF689
100 µg
$355
GLI-1
Human/Mouse
None
388516
Rat IgG2A
MAB3324
100 µg
$240
GLI-1 aa 1-234
Human
None
Polyclonal
Goat IgG
AF3324
100 µg
$355
GLI-1 aa 1-237
Human/Mouse
None
Polyclonal
Goat IgG
AF3455
100 µg
$355
GLI-2
Human
None
Polyclonal
Sheep IgG
AF3526
100 µg
$355
GLI-2 aa 1-416
Mouse
Biotin
Polyclonal
Sheep IgG
BAF3526
50 µg
$380
None
Polyclonal
Goat IgG
AF3635
100 µg
$355
GLI-3 aa 1-479
Human/Mouse
None
Polyclonal
Goat IgG
AF3690
100 µg
$355
GLP/EHMT1
Human/Mouse
None
B0422
Mouse IgG2A
PP-B0422-00
100 µg
$380
GLP-1R
Human
None
419208
Mouse IgG2A
MAB28141
100 µg
$240
GLP-2R
Human
None
413801
Mouse IgG2A
MAB4285
100 µg
$240
Glucagon
Human/Mouse
None
181402
Mouse IgG2A
MAB1249
100 µg
$240
Glucagon in bTC6 Cells. Glucagon expression was detected in bTC6 mouse insulinoma cells using antihuman/mouse Glucagon monoclonal antibody (Catalog # MAB1249). The cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody ( Catalog # NL007; red) and counterstained with DAPI (blue).
Glucagon NorthernLights-conjugated
Human/Mouse
NL557
181402
Mouse IgG2A
NL1249R
500 µL
$345
Glut1
Human
None
202915
Mouse IgG2B
MAB1418
200 µg
$270
Human
None
199017
Mouse IgG2A
MAB1414
200 µg
$270
Biotin
205115
Rat IgG2B
BAM1440
200 µg
$380
None
205115
Rat IgG2B
MAB1440
200 µg
$270
Biotin
202017
Mouse IgG2B
BAM1415
200 µg
$315
Glut2
Glut3
Mouse
Human
Glut4
Rat
Glut5
Human
54
None
202017
Mouse IgG2B
MAB1415
200 µg
$270
Biotin
1F8
Mouse IgG1
BAM1262
250 µg
$380
None
1F8
Mouse IgG1
MAB1262
200 µg
$270
Biotin
195205
Mouse IgG2A
BAM1349
200 µg
$380
None
195205
Mouse IgG2A
MAB1349
200 µg
$270
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Glutathione Peroxidase 1
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF3798
100 µg
$355
Polyclonal
Goat IgG
AF4199
100 µg
$355
GPX1 in Rat Liver. Glutathione Peroxidase 1 (GPX1) was detected in a cryostat section of rat liver using anti-human/mouse/rat GPX1 affinity-purified polyclonal antibody (Catalog # AF3798). The tissue was stained using the anti-goat Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Glutathione Peroxidase 3 aa 74-226
Human/Mouse/Rat
None
GPX3 in Human Kidney. Glutathione Peroxidase 3 (GPX3) was detected in paraffin-embedded human kidney sections using anti-human GPX3 affinity-purified polyclonal antibody (Catalog # AF4199). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Glycine R Pan Specific Glycophorin A Glypican 1
Glypican 2
Rat Human
Polyclonal
Rabbit IgG
PPS009
200 µg
$355
R10
Mouse IgG1
BAM12281
250 µg
$380
None
R10
Mouse IgG1
MAB1228
500 µg
$325
Human
None
Polyclonal
Goat IgG
AF4519
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF2304
100 µg
$355
None
Polyclonal
Goat IgG
AF2355
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2355
50 µg
$380
None
300736
Rat IgG2A
MAB2355
100 µg
$240
None
Polyclonal
Sheep IgG
AF2119
100 µg
$355
Mouse
Glypican 3
Human
Glypican 6
Mouse
GM-CSF
None Biotin
Canine
None
Polyclonal
Goat IgG
AF1053
100 µg
$355
None
Polyclonal
Goat IgG
AF1546
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1546
50 µg
$380
Human
None
3209
Mouse IgG1
MAB215
500 µg
$325
GMF-b
Human
None
Polyclonal
Goat IgG
AF1276
100 µg
$355
Goosecoid
Human
None
Polyclonal
Goat IgG
AF4086
100 µg
$355
gp130
Mouse
Biotin
125623
Rat IgG2A
BAM4681
100 µg
$240
None
125623
Rat IgG2A
MAB4681
100 µg
$240
419859
Mouse IgG2A
MAB4617
100 µg
$240
gp130 in Mouse Splenocytes. gp130 was detected in mouse splenocytes using anti-mouse gp130 monoclonal antibody (Catalog # MAB4681; red). Cells were counterstained with DAPI (blue).
GPR34
Human
None
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
55
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE GPR50
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human
None
461129
Mouse IgG2A
MAB4645
100 µg
$240
None
481906
Mouse IgG2A
MAB5239
100 µg
$240
None
H8004
Mouse IgG2A
PP-H8004-00
100 µg
$380
None
H8031
Mouse IgG2A
PP-H8031-00
100 µg
$380
Biotin
RB6-8C5
Rat IgG2B
BAM1037
250 µg
$380
GPR50 in Human Glioblastoma Cells. G proteincoupled receptor 50 (GPR50) was detected in human A172 glioblastoma cell line using anti-human GPR50 monoclonal antibody (Catalog # MAB4645). Cells were stained using NorthernLights-557 donkey antimouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
GPRC5A
Human
GR/NR3C1
Human
Gr-1/Ly-6G
Mouse
Granzyme A
Human
None
RB6-8C5
Rat IgG2B
MAB1037
500 µg
$325
None
356412
Mouse IgG2B
MAB29051
100 µg
$240
None
351927
Mouse IgG2A
MAB2906
100 µg
$240
None
Polyclonal
Goat IgG
AF1865
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1865
50 µg
$380
None
Polyclonal
Goat IgG
AF1365
100 µg
$355
None
Polyclonal
Goat IgG
AF1377
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1377
50 µg
$380
None
Polyclonal
Goat IgG
AF3846
100 µg
$355
None
Polyclonal
Goat IgG
AF956
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF956
50 µg
$380
None
Polyclonal
Sheep IgG
AF4339
100 µg
$355
None
Polyclonal
Goat IgG
AF1067
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1067
50 µg
$380
None
Polyclonal
Goat IgG
AF1566
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1566
50 µg
$380
None
222517
Mouse IgG2B
MAB15661
500 µg
$325
Granzyme A in Human Peripheral Blood Mononuclear Cells. Granzyme A was detected in human peripheral blood mononuclear cells using antihuman Granzyme A monoclonal antibody (Catalog # MAB29051). Cells were stained using donkey antimouse NorthernLights-557 secondary antibody (Catalog # NL007; red) and counterstained green.
Human Granzyme B
Mouse
Granzyme D
Mouse
Granzyme H
Human
GRB2
Human
GRB2 in Human Breast Cancer. Growth Factor Receptor Bound Protein 2 (GRB2) was detected in paraffin-embedded human breast cancer sections using anti-human/mouse/rat GRB2 affinity-purified polyclonal antibody (Catalog # AF3846). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown). Tissue was counterstained with hematoxylin (blue).
Gremlin
Mouse
GRK2 aa 468-689
Human/Mouse/Rat Human
Growth Hormone Rat
56
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF1210
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1210
50 µg
$380
None
Polyclonal
Goat IgG
AF1360
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1360
50 µg
$380
Rat
None
Polyclonal
Goat IgG
AF1211
100 µg
$355
Human
None
419605
Mouse IgG1
MAB35841
100 µg
$240
Human Growth Hormone R/GHR
GRP75/HSPA9B
Mouse
GRP75 in Human Fibroblastic Meningioma. Glucose Regulated 75 kDa Protein (GRP75) was detected in a paraffin-embedded human fibroblastic meningioma section using anti-human GRP75 monoclonal antibody (Catalog # MAB35841). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
GRP75/HSPA9B aa 542-679
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF3584
100 µg
$355
GSK-3a aa 90-483
Human
None
288901
Mouse IgG2B
MAB2986
100 µg
$240
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF4125
100 µg
$285
Phospho-GSK-3a (S21)
Phosphorylated GSK-3a in Human Thymus. Phosphorylated Glycogen Synthase kinase-3a (GSK-3a) was detected in paraffin-embedded human thymus tissue sections using anti-human/mouse/rat Phospho-GSK-3a (S27) affinity-purified polyclonal antibody (Catalog # AF4125). Tissues were stained using anti-rabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue).
Phospho-GSK-3a/b (S21/S9)
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF1590
100 µg
$285
GSK-3b
Human
None
272535
Rat IgG2B
MAB25061
100 µg
$240
HAI-1 aa 30-433
Mouse
None
Polyclonal
Goat IgG
AF1141
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1141
50 µg
$380
None
Polyclonal
Goat IgG
AF1048
100 µg
$355
HAI-1 Ectodomain
HAI-2 Ectodomain
Mouse
Hairless
Human
HAND1
Human
HAND2
Human/Mouse
Biotin
Polyclonal
Goat IgG
BAF1048
50 µg
$380
None
Polyclonal
Goat IgG
AF1107
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1107
50 µg
$380
None
Polyclonal
Goat IgG
AF5708
100 µg
$355
None
Polyclonal
Goat IgG
AF3168
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF3168
50 µg
$380
None
Polyclonal
Goat IgG
AF3876
100 µg
$355
HAPLN4
Human
None
Polyclonal
Goat IgG
AF4085
100 µg
$355
HB-EGF
Human
None
Polyclonal
Goat IgG
AF-259-NA
250 µg
$355
HCRTR2
Human
None
456738
Mouse IgG2A
MAB5246
100 µg
$240
Hepassocin
Rat
None
456723
Mouse IgG2B
MAB52461
100 µg
$240
None
Polyclonal
Goat IgG
AF1105
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1105
50 µg
$380
HES-1 aa 93-280
Human
None
Polyclonal
Goat IgG
AF3317
100 µg
$355
HES-4 aa 94-221
Human
None
Polyclonal
Sheep IgG
AF3600
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF-294-NA
100 µg
$355
HGF HGF Activator
Human
Mouse
None
Polyclonal
Goat IgG
AF2207
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF1514
100 µg
$355
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
57
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES Human
HGF R/c-MET Mouse Phospho-HGF R/c-MET (Y1003)
Human
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF276
100 µg
$355
None
95308
Mouse IgG1
MAB3583
100 µg
$240
None
Polyclonal
Goat IgG
AF527
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF527
50 µg
$380
None
Polyclonal
Rabbit IgG
AF4059
100 µg
$285
Phospho-HGF R/c-MET (Y1234/Y1235)
Human
None
Polyclonal
Rabbit IgG
AF2480
50 µg
$285
Phospho-HGF R/c-MET (Y1349)
Human
None
Polyclonal
Rabbit IgG
AF3950
50 µg
$285
HIF-1a aa 575-826
Human/Mouse
None
241812
Mouse IgG1
MAB1935
100 µg
$240
HIF-2a aa 542-874
Mouse
None
Polyclonal
Goat IgG
AF2997
100 µg
$355
Phospho-Histone H2AX (S139)
Human
None
Polyclonal
Rabbit IgG
AF2288
50 µg
$285
HMGA2
Human
None
Polyclonal
Goat IgG
AF3184
100 µg
$355
HNF-3b/FoxA2 aa 242-457
Human
None
Polyclonal
Goat IgG
AF2400
100 µg
$355
None
H1415
Mouse IgG2A
PP-H1415-00
100 µg
$380
None
K9218
Mouse IgG2A
PP-K9218-00
100 µg
$380
PP-N3224-00
100 µg
$380
HNF-3b in Human BG01V Cells. Hepatic Nuclear Factor 3 b (HNF-3b) was detected in endoderm differentiated human BG01V embryonic stem cell line using anti-human HNF-3b affinity-purified polyclonal antibody (Catalog # AF2400). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
HNF-4a/NR2A1
Human
HNF-4g/NR2A2
Human
None
N3224
Mouse IgG2A
HNF-4a/NR2A1 Isoform 7-9
Human
None
H6939
Mouse IgG1
PP-H6939-00
100 µg
$380
HOXA1
Human
None
Polyclonal
Goat IgG
AF5014
100 µg
$355
HSP60
Human/Mouse/Rat
None
264233
Mouse IgG2B
MAB1800
100 µg
$240
HSP70
Human/Mouse
None
242707
Mouse IgG2A
MAB1663
100 µg
$240
HSPH1 aa 595-733
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF4029
100 µg
$355
229926
Mouse IgG2B
MAB1458
100 µg
$240 $285
HSPH1 in Rat Cerebellum. HSP (Heat Shock Protein) H1 was detected in a cryostat section of rat cerebellum using anti-human/mouse/rat HSPHI affinitypurified polyclonal antibody (Catalog # AF4029). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
HTRA2/Omi
Human
None
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF1458
100 µg
HVEM/TNFRSF14
Human
None
94804
Mouse IgG1
MAB3561
500 µg
$325
cIAP-1/HIAP-2
Human
None
Polyclonal
Goat IgG
AF8181
100 µg
$355
cIAP-2/HIAP-1
Human
None
Polyclonal
Goat IgG
AF8171
100 µg
$355
None
315304
Mouse IgG1
MAB817
100 µg
$240
ICA1
Human
None
Polyclonal
Goat IgG
AF4084
100 µg
$355
None
Polyclonal
Goat Serum
BBA17
500 µL
$195
None
BBIG-I1
Mouse IgG1
BBA3
100 µg
$230
None
BBIG-I1
Mouse IgG1
BBA4
1 mg
$840
None
Polyclonal
Goat IgG
AF796
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF796
50 µg
$380
None
Polyclonal
Goat IgG
AF583
100 µg
$355
Human ICAM-1/CD54 Mouse Rat
58
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF774
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF774
50 µg
$380 $315
ICAM-2/CD102
Mouse
ICAM-3/CD50
Human
None
ICAM-3.3
Mouse IgG1
BBA15
200 µg
ICAM-5
Human
None
283607
Mouse IgG2A
MAB1950
100 µg
$240
None
Polyclonal
Goat IgG
AF1173
100 µg
$355
ICAM-5
Mouse
ICOS
Human
Biotin
Polyclonal
Goat IgG
BAF1173
None
Polyclonal
Goat IgG
AF169
50 µg
$380
100 µg
$355
ID2
Human
None
Polyclonal
Goat IgG
AF4660
100 µg
$355
IFITM1
Human
None
Polyclonal
Goat IgG
AF4827
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF3377
100 µg
$355
None
MMHA-3
Mouse IgG1
21101-1
50 µg
$195
None
MMHA-1
Mouse IgG1
21105-1
50 µg
$195
None
MMHA-8
Mouse IgG2B
21110-1
50 µg
$195
None
MMHA-11
Mouse IgG1
21112-1
50 µg
$250
Fluorescein
MMHA-11
Mouse IgG1
21112-3
50 Tests
$300
None
MMHA-13
Mouse IgG1
21116-1
50 µg
$195
None
MMHA-17
Mouse IgG1
21118-1
50 µg
$195
None
MMHA-6
Mouse IgG1
21125-1
50 µg
$195
None
MMHA-9
Mouse IgG1
21127-1
50 µg
$195
None
MMHA-14
Mouse IgG1
21129-1
50 µg
$195
None
Polyclonal
Sheep Serum
31100-1
100000 U
$235
None
Polyclonal
Rabbit Serum
31101-1
100000 U
$235
None
Polyclonal
Rabbit Serum
31130-1
20000 U
$215
Fluorescein
RMMA-1
Rat IgG1
22100-3
100 µL
$355
IFITM3/Fragilis
IFITM3/Fragilis in Embryonic Mouse Ovary. Interferon-induced transmembrane protein 3 (IFITM3) was detected in an E13.5 mouse embryonic ovary section using anti-mouse IFITM3 affinity-purified polyclonal antibody (Catalog # AF3377). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
Human
IFN-a
Mouse
Porcine
None
Polyclonal
Rabbit Serum
32100-1
20000 U
$325
None
K9
Mouse IgG1
27100-1
500 µg
$315
None
F17
Mouse IgG1
27105-1
500 µg
$315
IFN-a/b R1
Mouse
Biotin
Polyclonal
Goat IgG
BAF3039
50 µg
$380
Phospho-IFN-a/b R1 (S535/S539)
Human/Mouse/Rat/Bovine/ Canine/Primate/Sheep
None
Polyclonal
Rabbit IgG
PPS074
100 µL
$355
IFN-a/b R2
Human
None
MMHAR-2
Mouse IgG2A
21385-1
50 µg
$330
None
Polyclonal
Rabbit Serum
31385-1
50 µg
$250
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
59
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES
Human IFN-b
Mouse
Bovine
Canine Equine IFN-g Human
Mouse
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Fluorescein
MMHB-3
Mouse IgG1
21400-3
50 Tests
$270
None
MMHB-1
Mouse IgG2A
21405-1
50 µg
$195
None
MMHB-12
Mouse IgG1
21450-1
50 µg
$195
None
MMHB-13
Mouse IgG3
21455-1
50 µg
$195
None
MMHB-14
Mouse IgG2A
21460-1
50 µg
$195
None
MMHB-15
Mouse IgG2B
21465-1
50 µg
$195
None
MMHB-16
Mouse IgG1
21470-1
50 µg
$195
None
Polyclonal
Sheep Serum
31400-1
20000 U
$280
None
Polyclonal
Rabbit Serum
31410-1
20000 U
$225
Fluorescein
RMMB-1
Rat IgG1
22400-3
100 µL
$320
None
Polyclonal
Rabbit Serum
32400-1
20000 U
$315
None
Polyclonal
Goat IgG
AF2300
100 µg
$355
None
345025
Rat IgG2A
MAB2300
100 µg
$240
None
Polyclonal
Goat IgG
AF781
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF781
50 µg
$380
None
Polyclonal
Goat IgG
AF1586
100 µg
$355
None
Polyclonal
Goat IgG
AF-285-NA
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF285
50 µg
$380
None
25718
Mouse IgG2A
MAB285
500 µg
$325
None
25723
Mouse IgG2B
MAB2851
500 µg
$325
None
H22
Hamster IgG
MAB4851
500 µg
$325
Porcine
Biotin
Polyclonal
Goat IgG
BAF985
50 µg
$380
Rat
None
Polyclonal
Goat IgG
AF-585-NA
100 µg
$355
None
Polyclonal
Goat IgG
AF673
100 µg
$355
NL557
Polyclonal
Donkey IgG
NL001
500 µL
$115
NL637
Polyclonal
Donkey IgG
NL002
500 µL
$115
NL493
Polyclonal
Donkey IgG
NL003
500 µL
$115
NL557
Polyclonal
Donkey IgG
NL007
500 µL
$115
NL637
Polyclonal
Donkey IgG
NL008
500 µL
$115
NL493
Polyclonal
Donkey IgG
NL009
500 µL
$115
NL557
Polyclonal
Donkey IgG
NL004
500 µL
$115
NL637
Polyclonal
Donkey IgG
NL005
500 µL
$115
NL493
Polyclonal
Donkey IgG
NL006
500 µL
$115
NL557
Polyclonal
Goat IgG
NL013
500 µL
$115
NL637
Polyclonal
Goat IgG
NL014
500 µL
$115
NL493
Polyclonal
Goat IgG
NL015
500 µL
$115
NL557
Polyclonal
Donkey IgG
NL010
500 µL
$115
NL637
Polyclonal
Donkey IgG
NL011
500 µL
$115
NL493
Polyclonal
Donkey IgG
NL012
500 µL
$115
IFN-g in Human Peripheral Blood Mononuclear Cells (PBMC). Interferon g (IFN-g) was detected in PMA-, ionomyocin-, and monensin-activated human PBMC using anti-human IFN-g affinity-purified polyclonal antibody (Catalog # AF-285-NA). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
IFN-g R1/CD119
Human
Goat
Mouse
IgG NorthernLights-conjugated
Rabbit
Rat
Sheep
IgG2A
Mouse
None
133304
Mouse IgG2A
MAB0031
500 µg
$170
IgG2B
Mouse
None
73009
Mouse IgG2B
MAB0042
500 µg
$170
60
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
NL557
Polyclonal
Goat IgG
NL016
500 µL
$115
Polyclonal
Goat IgG
NL017
500 µL
$115
IgY NorthernLights-conjugated
Chicken
NL637 NL493
Polyclonal
Goat IgG
NL018
500 µL
$115
IGFBP-1
Mouse
None
Polyclonal
Goat IgG
AF1240
100 µg
$355
IGFBP-3
Human
None
Polyclonal
Goat IgG
AF675
100 µg
$355
None
84728
Mouse IgG2B
MAB305
500 µg
$325
None
Polyclonal
Goat IgG
AF804
100 µg
$355
IGFBP-4
IGFBP-5
Human
Human Mouse
Biotin
Polyclonal
Goat IgG
BAF804
50 µg
$380
None
Polyclonal
Goat IgG
AF875
100 µg
$355
None
164503
Mouse IgG2B
MAB875
500 µg
$325
None
Polyclonal
Goat IgG
AF578
100 µg
$355
IGFBP-5 in Mouse Ovary. Insulin-like Growth Factor Binding Protein 5 (IGFBP-5) was detected in a cryostat section of mouse ovary (follicle with oocyte) using anti-mouse IGFBP-5 affinity-purified polyclonal antibody (Catalog # AF578). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
IGFBP-6 IGFBP-L1
IGFBP-rp1/IGFBP-7
IGF-I IGF-I R IGF-II
Mouse Mouse
Human
Polyclonal
Goat IgG
AF776
100 µg
$355
Polyclonal
Goat IgG
AF4130
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF4130
50 µg
$380
None
Polyclonal
Goat IgG
AF1334
100 µg
$355
50 µg
$380
Biotin
Polyclonal
Goat IgG
BAF1334
Mouse
None
292917
Rat IgG1
MAB21201
100 µg
$240
Human
None
Polyclonal
Goat IgG
AF-291-NA
250 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF791
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF-305-NA
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF-292-NA
250 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF792
100 µg
$355
IGF-II R
Human
None
Polyclonal
Goat IgG
AF2447
100 µg
$355
IGSF2/CD101
Mouse
Biotin
307707
Rat IgG2A
BAM3368
100 µg
$380
IGSF4B
Human
None
Polyclonal
Goat IgG
AF3678
100 µg
$355
None
404619
Rat IgG1
MAB4164
100 µg
$240
None
404617
Rat IgG1
MAB41641
100 µg
$240
None
Polyclonal
Goat IgG
AF3117
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF3117
50 µg
$380
IGSF4C
Human
IGSF8
Mouse
Ikaros
Human
None
Polyclonal
Goat IgG
AF4984
100 µg
$355
IKKa aa 375-745
Human/Mouse/Rat
None
Polyclonal
Sheep IgG
AF3768
100 µg
$355
IKKg IKKe
IL-1a/IL-1F1
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF2684
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF3199
100 µg
$355
Human/Mouse/Rat
None
346909
Mouse IgG1
MAB3199
100 µg
$240
None
Polyclonal
Goat IgG
AF-200-NA
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF200
50 µg
$380
None
4414
Mouse IgG2A
MAB200
500 µg
$325
None
Polyclonal
Goat IgG
AF-400-NA
100 µg
$355
Human
Mouse
None None
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
61
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES Feline
Human IL-1b/IL-1F2
Mouse
Rat
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF1796
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1796
50 µg
$380
None
Polyclonal
Total Goat IgG
AB-201-NA
1 mg
$315
None
Polyclonal
Goat IgG
AF-201-NA
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF201
50 µg
$380
None
8516
Mouse IgG1
MAB201
500 µg
$325
None
2805
Mouse IgG1
MAB601
500 µg
$325
None
Polyclonal
Goat IgG
AF-401-NA
100 µg
$355
None
Polyclonal
Goat IgG
AF-501-NA
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF501
50 µg
$380
None
38139
Mouse IgG1
MAB501
500 µg
$325
IL-1b in Human Peripheral Blood Mononuclear Cells (PBMCs). Interleukin-1b (IL-1b) was detected in LPS-stimulated human PBMCs using anti-human IL-1b affinity-purified polyclonal antibody (Catalog # AF-201-NA). The cells were stained using NorthernLights-557 anti-goat secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
IL-1b/IL-1F2 NorthernLights-conjugated
IL-1ra/IL-1F3
IL-1F5/FIL1d
Human
NL557
Polyclonal
Goat IgG
NL201R
500 µL
$350
Equine
None
Polyclonal
Goat IgG
AF2466
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF-280-NA
100 µg
$355
Porcine
None
Polyclonal
Goat IgG
AF780
100 µg
$355
Human
IL-1F6/FIL1e
Human
IL-1F8/FIL1η
Human
IL-1 RI
Mouse
IL-1 RII
IL-1b in Rat Splenocytes. Interleukin 1b (IL-1b) was detected in LPS-stimulated rat splenocytes using anti-rat IL-1b affinity-purified polyclonal antibody (Catalog # AF-501-NA). Cells were stained using NorthernLights-557 anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained (green).
Human Mouse
None
Polyclonal
Goat IgG
AF1275
100 µg
$355
None
Polyclonal
Goat IgG
AF1078
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1078
50 µg
$380
None
Polyclonal
Goat IgG
AF1099
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1099
50 µg
$380
None
Polyclonal
Goat IgG
AF771
100 µg
$355
None
Polyclonal
Goat IgG
AF-263-NA
100 µg
$355
None
32437
Mouse IgG2A
MAB263
500 µg
$325
None
Polyclonal
Goat IgG
AF563
100 µg
$355
Polyclonal
Goat IgG
AF1007
100 µg
$355
IL-1 RII in Human Peripheral Blood Mononuclear Cells (PBMCs). Interleukin-1 receptor II (IL-1 RII) was detected in human PBMCs by indirect immunofluorescent staining using anti-human IL-1 RII affinitypurified polyclonal antibody (Catalog # AF-263-NA) and counterstained (green).
IL-1 RAPL2/IL-1 R9
62
Human
None
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Bovine
None
Polyclonal
Goat IgG
AF2465
100 µg
$355
Canine
Biotin
Polyclonal
Goat IgG
BAF1815
50 µg
$380
None
Polyclonal
Goat IgG
AF1613
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1613
50 µg
$380
None
Polyclonal
Goat IgG
AF1890
100 µg
$355
Equine
Feline IL-2 Human
Porcine Rat
Biotin
Polyclonal
Goat IgG
BAF1890
None
Polyclonal
Goat IgG
AF-202-NA
Biotin
Polyclonal
Goat IgG
None
5334
Mouse IgG1
SIZE
PRICE
50 µg
$380
100 µg
$355
BAF202
50 µg
$380
MAB202
500 µg
$325
None
5355
Mouse IgG2A
MAB602
500 µg
$325
None
Polyclonal
Goat IgG
AF652
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF652
50 µg
$380
None
Polyclonal
Goat IgG
AF-502-NA
100 µg
$355
None
22722
Mouse IgG1
MAB223
500 µg
$325
None
24204
Mouse IgG1
MAB623
500 µg
$325
IL-2 in Human Peripheral Blood Mononuclear Cells (PBMCs). Interleukin-2 (IL-2) was detected in PMA- and ionomycin-stimulated human PBMCs using biotinylated anti-human IL-2 affinity-purified polyclonal antibody (Catalog # BAF202; red) and counterstained green.
Human IL-2 Ra
Mouse
None
Polyclonal
Goat IgG
AF2438
100 µg
$355
Rat
None
Polyclonal
Sheep IgG
AF5156
100 µg
$355
IL-2 Ra in Mouse Spleen. Interleukin-2 Receptor a (IL-2 Ra) was detected in cryostat tissue sections of mouse spleen using anti-mouse IL-2 Ra affinity-purified polyclonal antibody (Catalog # AF2438). Tissues were stained using NorthernLights-557 donkey antigoat IgG secondary antibody (Catalog # NL001; red). Tissues were counterstained green.
IL-2 Rb
Human
Human IL-3 Rat IL-3 Ra
Human
None
27302
Mouse IgG1
MAB224
500 µg
$325
None
Polyclonal
Total Goat IgG
AB-203-NA
1 mg
$355
None
Polyclonal
Goat IgG
AF-203-NA
100 µg
$355
None
4806
Mouse IgG1
MAB203
500 µg
$325
None
Polyclonal
Goat IgG
AF2524
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2524
50 µg
$380
None
32703
Mouse IgG1
MAB301
500 µg
$325
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
63
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
IL-4
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Bovine
None
Polyclonal
Goat IgG
AF2469
100 µg
$355
Equine
None
Polyclonal
Goat IgG
AF1809
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF204
50 µg
$380
None
3007
Mouse IgG1
MAB304
500 µg
$325
Porcine
None
Polyclonal
Goat IgG
AF654
100 µg
$355
Rat
Biotin
Polyclonal
Goat IgG
BAF504
50 µg
$380
Human
SIZE
PRICE
IL-4 in Human Lymph Node. Interleukin-4 (IL-4) was detected in a cryostat tissue section of frozen human lymph node using anti-human IL-4 monoclonal antibody (Catalog # MAB304). Tissues were stained using anti-mouse secondary antibody (brown) and counterstained with hematoxylin (blue).
IL-4 Ra
IL-5
Human
None
25463
Mouse IgG2A
MAB230
500 µg
$325
Canine
None
Polyclonal
Goat IgG
AF1964
100 µg
$355
Equine
None
Polyclonal
Goat IgG
AF2470
100 µg
$355
Feline
None
Polyclonal
Goat IgG
AF1795
100 µg
$355
Human
None
9906
Mouse IgG1
MAB605
500 µg
$325
Mouse
None
Polyclonal
Goat IgG
AF-405-NA
100 µg
$355
Porcine
None
Polyclonal
Goat IgG
AF3137
100 µg
$355
None
Polyclonal
Goat IgG
AF545
100 µg
$355
None
299298
Mouse IgG1
MAB5452
100 µg
$240
None
Polyclonal
Goat IgG
AF1609
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1609
50 µg
$380
None
247002
Mouse IgG2B
MAB16091
500 µg
$325
Rat
Canine
Equine Feline IL-6 Human
None
Polyclonal
Goat IgG
AF1886
100 µg
$355
None
Polyclonal
Goat IgG
AF2305
100 µg
$355
None
341031
Mouse IgG2B
MAB23051
100 µg
$240
None
Polyclonal
Goat IgG
AF-206-NA
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF206
50 µg
$380
None
1936
Mouse IgG2B
MAB2061
500 µg
$325 $355
Mouse
None
Polyclonal
Goat IgG
AF-406-NA
100 µg
Porcine
None
77830
Mouse IgG1
MAB686
500 µg
$325
Rat
None
Polyclonal
Goat IgG
AF506
100 µg
$355
None
Polyclonal
Goat IgG
AF1830
100 µg
$355
Biotin
255821
Rat IgG2A
BAM1830
100 µg
$380
None
255821
Rat IgG2A
MAB1830
500 µg
$325
None
255820
Rat IgG1
MAB18301
100 µg
$240
IL-6 in Porcine Peripheral Blood Mononuclear Cells. Interleukin-6 (IL-6) was detected in porcine PBMCs using anti-porcine IL-6 monoclonal antibody (Catalog # MAB686). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
IL-6 Ra
64
Mouse
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
IL-7
MOLECULE
Human
None
7417
Mouse IgG1
MAB207
500 µg
$325
CXCR1/IL-8 RA
Human
None
42705
Mouse IgG2A
MAB330
500 µg
$325
CXCR2/IL-8 RB
Human
None
48311
Mouse IgG2A
MAB331
500 µg
$325
Equine
None
Polyclonal
Goat IgG
AF1605
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF-217-NA
100 µg
$355
None
Polyclonal
Goat IgG
AF693
100 µg
$355
None
262715
Mouse IgG2B
MAB6932
100 µg
$240
Rat
None
Polyclonal
Goat IgG
AF519
100 µg
$355
IL-10 Ra
Human
None
Polyclonal
Goat IgG
AF-274-NA
100 µg
$355
None
Polyclonal
Goat IgG
AF-219-NA
100 µg
$355
IL-12
Human
Biotin
Polyclonal
Goat IgG
BAF219
100 µg
$380
None
24910
Mouse IgG1
MAB219
500 µg
$325
IL-10
Porcine
IL-12 p70
Human
None
24945
Mouse IgG1
MAB611
500 µg
$325
IL-12/IL-23 p40
Feline
None
Polyclonal
Goat IgG
AF1954
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF-413-NA
100 µg
$355
Rat
Biotin
296202
Mouse IgG2B
BAM1945
100 µg
$380
Human
None
Polyclonal
Goat IgG
AF152
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF146
100 µg
$355
IL-13 IL-13 Ra1 IL-13 Ra2
IL-15
IL-15 Ra
Mouse
None
Polyclonal
Goat IgG
AF539
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF315
100 µg
$355
None
Polyclonal
Goat IgG
AF447
100 µg
$355
Mouse Human
Biotin
Polyclonal
Goat IgG
BAF447
50 µg
$380
None
Polyclonal
Goat IgG
AF247
100 µg
$355
IL-16 C-terminal Peptide
Human
None
Polyclonal
Goat IgG
AF-316-PB
100 µg
$355
IL-17
Human
None
Polyclonal
Goat IgG
AF-317-NA
100 µg
$355
None
Polyclonal
Goat IgG
AF1207
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1207
50 µg
$380
None
Polyclonal
Goat IgG
AF1040
100 µg
$355
IL-17B R
Human Mouse
IL-17C IL-17D
Human Human
None
Polyclonal
Goat IgG
AF1234
100 µg
$355
None
Polyclonal
Goat IgG
AF1504
100 µg
$355
None
246018
Mouse IgG2B
MAB15041
100 µg
$240
IL-17D in Human Heart. Interleukin-17D (IL-17D) was detected in a paraffin-embedded human heart section using anti-human IL-17D monoclonal antibody (Catalog # MAB15041). The tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
IL-17F
Human
None
Polyclonal
Goat IgG
AF1335
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF2057
100 µg
$355
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
65
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
IL-17 RD/SEF
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Human
None
Polyclonal
Goat IgG
AF2275
100 µg
$355
None
Polyclonal
Goat IgG
AF2276
100 µg
$355
None
400210
Rat IgG2A
MAB2276
100 µg
$240
Mouse
IL-17 RD in Mouse Liver. Interleukin-17 Receptor D (IL-17 RD) was detected in frozen mouse liver sections using anti-mouse IL-17 RD monoclonal antibody (Catalog # MAB2276; red) and counterstained green.
Canine IL-18/IL-1F4
IL-18 Ra/IL-1 R5
SIZE
PRICE
IL-17 RD in Human Kidney. Interleukin-17 Receptor D (IL-17 RD) was detected in a paraffin-embedded human kidney sections using anti-human IL-17 RD affinity-purified polyclonal antibody (Catalog # AF2275). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
None
Polyclonal
Goat IgG
AF2924
100 µg
$355
None
314811
Mouse IgG1
MAB29241
100 µg
$240
Porcine
None
Polyclonal
Goat IgG
AF588
100 µg
$355
Rhesus Macaque
None
Polyclonal
Goat IgG
AF2548
100 µg
$355
None
Polyclonal
Goat IgG
AF840
100 µg
$355
None
70625
Mouse IgG1
MAB840
500 µg
$325
Human
IL-18 R in Human Skin. Interleukin-18 Receptor (IL18 R) was detected in frozen sections of human skin using anti-human IL-18 R affinity-purified polyclonal antibody (Catalog # AF840). Tissue was stained using the anti-goat HRP-AEC Cell and Tissue Staining Kit (Catalog # CTS009; red) and counterstained with hematoxylin (blue).
IL-20 Ra
Human
None
173707
Mouse IgG1
MAB11761
500 µg
$325
IL-21
Mouse
None
Polyclonal
Goat IgG
AF594
100 µg
$355
IL-21 in Mouse Splenocytes. Interleukin-21 (IL-21) was detected in mouse splenocytes stimulated with concanavalin A using anti-mouse IL-21 affinity-purified polyclonal antibody (Catalog # AF594). Cells were stained using NorthernLights-557 anti-goat secondary antibody (Catalog # NL001; red) and counterstained green.
IL-21 R Subunit
Mouse
None
Polyclonal
Goat IgG
AF596
100 µg
$355
IL-22
Human
None
Polyclonal
Goat IgG
AF782
100 µg
$355
Human
None
305405
Mouse IgG1
MAB2770
100 µg
$240 $240
IL-22 Ra1
IL-24
Mouse
None
496514
Rat IgG2A
MAB42941
100 µg
Human
None
283123
Mouse IgG2B
MAB19651
100 µg
$240
None
Polyclonal
Goat IgG
AF2786
100 µg
$355
Mouse
Biotin
303308
Rat IgG2A
BAM2786
100 µg
$380
IL-27 p28
Mouse
None
Polyclonal
Goat IgG
AF1834
100 µg
$355
IL-28A/IFN-λ2
Human
None
Polyclonal
Goat IgG
AF1587
100 µg
$355
IL-31
Human
None
Polyclonal
Goat IgG
AF2824
100 µg
$355
None
308202
Mouse IgG1
MAB28241
100 µg
$240
IL-31 RA
Human
None
Polyclonal
Goat IgG
AF2769
100 µg
$355
66
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
IL-33
IL-33 Propeptide
SPECIES Human
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF3625
100 µg
$355
None
390412
Rat IgG2B
MAB3625
100 µg
$240
Mouse
None
Polyclonal
Goat IgG
AF3626
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF4810
100 µg
$355
Mouse
None
Polyclonal
Sheep IgG
AF5010
100 µg
$355
IL-33 Propeptide in Human Umbilical Vein Endothelial Cells. Interleukin-33 (IL-33) Propeptide was detected in HUVECs using anti-human IL-33 Propeptide affinity-purified polyclonal antibody (Catalog # AF4810). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
Indian Hedgehog N-Terminus
Mouse
None
Polyclonal
Goat IgG
AF1705
100 µg
$355
ING1
Human
None
Polyclonal
Goat IgG
AF5758
100 µg
$355
Insulin
Human/Mouse/Bovine
None
182410
Rat IgG2A
MAB1417
100 µg
$240
Proinsulin
Human/Mouse
Biotin
253627
Mouse IgG2A
BAM13361
100 µg
$240
None
253627
Mouse IgG2A
MAB13361
100 µg
$240
None
Polyclonal
Goat IgG
AF1544
100 µg
$355
None
243524
Mouse IgG2B
MAB1544
100 µg
$240
None
Polyclonal
Goat IgG
AF3055
100 µg
$355
Insulin R/CD220
Human
INSRR
Human
Integrin a1/CD49a
Human
Human Integrin a2/CD49b Mouse Integrin a2b/CD41 Integrin a3/CD49c
Integrin a4/CD49d
Mouse Human
Human
None
Polyclonal
Sheep IgG
AF5676
100 µg
$355
Biotin
HAS3
Mouse IgG2A
BAM1233
100 µg
$295
None
HAS3
Mouse IgG2A
MAB1233
500 µg
$325
None
430903
Rat IgG2A
MAB12332
100 µg
$240
None
Polyclonal
Sheep IgG
AF1740
100 µg
$355
Biotin
Polyclonal
Sheep IgG
BAF1740
50 µg
$380
None
386629
Rat IgG2A
MAB41181
100 µg
$240
Biotin
IA3
Mouse IgG1
BAM1345
100 µg
$295
None
IA3
Mouse IgG1
MAB1345
500 µg
$325
Biotin
7.2R
Mouse IgG1
BAM1354
100 µg
$295
None
7.2R
Mouse IgG1
MAB1354
500 µg
$325
Integrin a4/CD49d in Human U937 Cells. Integrin a4/CD49d was detected in human U937 lymphoma cell line using anti-human integrin a4/CD49d monoclonal antibody (Catalog # MAB1354). Cells were stained using NorthernLights-557 donkey antimouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
Integrin a5/CD49e
Human
None
238307
Mouse IgG1
MAB1864
100 µg
$240
Mouse
None
235112
Rat IgG2B
MAB1754
100 µg
$240
Integrin a5 in Human Fibrosarcoma Cell Line (HT1080). Integrin a5 was detected in HT1080 human fibrosarcoma cells using anti-human integrin a5 monoclonal antibody (Catalog # MAB1219). Cells were stained using NorthernLights-557 donkey antimouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
67
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Integrin a6/CD49f
Integrin a8
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Human
None
MP4F10
Mouse IgG2B
MAB1350
500 µg
$325
Human/Mouse/Bovine
None
GoH3
Rat IgG2A
MAB13501
100 µg
$240
None
Polyclonal
Goat IgG
AF4076
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF4076
50 µg
$380
Biotin
ICRF44
Mouse IgG1
BAM1699
100 µg
$240
None
ICRF44
Mouse IgG1
MAB1699
500 µg
$325
None
238446
Mouse IgG2B
MAB16991
100 µg
$240
Biotin
M1/70
Rat IgG2B
BAM1124
250 µg
$380
None
M1/70
Rat IgG2B
MAB1124
500 µg
$325
None
Polyclonal
Goat IgG
AF1219
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1219
50 µg
$380
None
P2W7
Mouse IgG1
MAB1219
500 µg
$325
Mouse
Human Integrin aM/CD11b Mouse
SIZE
PRICE
Integrin aV/CD51
Human
None
273210
Mouse IgG1
MAB12191
100 µg
$240
Integrin aVb3
Human
None
23C6
Mouse IgG1
MAB3050
100 µg
$240
Integrin aVb5
Human
None
P5H9
Mouse IgG1
MAB2528
100 µg
$240
Integrin b1/CD29
Human
None
4B7R
Mouse IgG1
MAB1778
100 µg
$240
None
P5D2
Mouse IgG1
MAB17781
100 µg
$240
None
P4G11
Mouse IgG1
MAB17782
100 µg
$240
Human Integrin b1/CD29 Mouse
None
419127
Mouse IgG2B
MAB17783
100 µg
$240
None
Polyclonal
Goat IgG
AF2405
100 µg
$355
None
265917
Rat IgG2A
MAB2405
100 µg
$240
Integrin b1, Orexin b, and p38a in Mouse Brainstem. Cryostat sections of mouse brainstem were labeled with anti-human Orexin B monoclonal antibody (Catalog # MAB734), anti-human/mouse/rat p38a affinitypurified polyclonal antibody (Catalog # AF8691), and anti-mouse Integrin b1 affinity-purified polyclonal antibody (Catalog #AF2405). Tissue was stained with NorthernLights (NL)-conjugated secondary polyclonal antibodies: NL-493 donkey anti-rabbit (Catalog # NL006; green), NL-557 donkey anti-mouse (Catalog # NL007; red), and NL-637 donkey anti-goat (Catalog # NL002; blue). Immunohistochemical image of integrin b1 expression is pseudo-colored for presentation.
Integrin b2/CD18
Integrin b4/CD104
Human Human Mouse
Integrin b5
Human
Human Integrin b6 Mouse
Integrin b7
68
Human
None
Polyclonal
Goat IgG
AF1730
100 µg
$355
None
Polyclonal
Sheep IgG
AF4060
100 µg
$355
None
422325
Mouse IgG2B
MAB4060
100 µg
$240
None
308601
Rat IgG2A
MAB4054
100 µg
$240
None
Polyclonal
Sheep IgG
AF3824
100 µg
$355
Biotin
Polyclonal
Sheep IgG
BAF3824
50 µg
$380
None
Polyclonal
Sheep IgG
AF4155
100 µg
$355
Biotin
Polyclonal
Sheep IgG
BAF4155
50 µg
$380
None
437211
Mouse IgG2B
MAB4155
100 µg
$240
None
Polyclonal
Goat IgG
AF2389
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2389
50 µg
$380
None
268622
Rat IgG2A
MAB2389
100 µg
$240
None
473207
Mouse IgG1
MAB4669
100 µg
$240
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Integrin b8
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human
None
416911
Mouse IgG1
MAB47751
100 µg
$240
Polyclonal
Sheep IgG
AF4254
100 µg
$355
Integrin b8 in Human A172 Cells. Integrin b8 was detected in regions of membrane ruffling in A172 human glioblastoma cells using anti-human Integrin b8 monoclonal antibody (Catalog # MAB4775). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
Intelectin-1/Omentin
Human
None
Intelectin-1 in Mouse Intestine. Intelectin-1 was detected in a section of mouse intestine using antimouse Intelectin-1 affinity-purified polyclonal antibody (Catalog # AF4254; red). The tissue was stained using NorthernLights-557 anti-sheep secondary antibody (Catalog # NL010) and the nuclei counterstained with DAPI (blue).
IRAK4
Human
None
Polyclonal
Goat IgG
AF3919
100 µg
$355
IRF3
Human
None
Polyclonal
Goat IgG
AF4019
100 µg
$355
IRF5 aa 214-395
Human
None
Polyclonal
Sheep IgG
AF4508
100 µg
$355
IRF8
Human
None
Polyclonal
Sheep IgG
AF5117
100 µg
$355
IRS-1
Human
None
395601
Mouse IgG1
MAB39781
100 µg
$240
ISG15/UCRP aa 2-157
Human
None
539442
Mouse IgG2B
MAB4845
100 µg
$240
Islet-1
Human
None
Polyclonal
Goat IgG
AF1837
100 µg
$355
Islet-1 NorthernLights-conjugated
Human
NL557
Polyclonal
Goat IgG
NL1837R
500 µL
$350
Islet-2
Human
None
Polyclonal
Sheep IgG
AF4244
100 µg
$355
Biotin
Polyclonal
Sheep IgG
BAF4244
50 µg
$380
Islet-2 and Plexin A3 in Embryonic Mouse Spinal Cord. Islet-2 and Plexin A3 were detected in a section of embryonic mouse spinal cord using biotinylated anti-human Islet-2 affinity-purified polyclonal antibody (Catalog # BAF4244) and anti-mouse Plexin A3 (Catalog # AF4075). The tissue was stained using NorthernLights-493 streptavidin (Catalog # NL997; green) and NorthernLights-557 donkey anti-goat IgG affinity-purified polyclonal antibody (Catalog # NL001; red), respectively. The nuclei were stained using DAPI (blue).
ISLR-2
Mouse
None
Polyclonal
Sheep IgG
AF4650
100 µg
$355
Jagged 1
Human
None
Polyclonal
Goat IgG
AF1277
100 µg
$355
Jagged 2
Human
None
Polyclonal
Goat IgG
AF1726
100 µg
$355
Jak1 aa 14-55
Mouse/Rat
None
Polyclonal
Goat IgG
AF602
100 µg
$355
Jak1 aa 20-274
Human/Mouse/Rat
None
413104
Rat IgG2B
MAB4260
100 µg
$240
Jak1 in Human Skin. Jak1 was detected in a section of paraffin-embedded human epidermis using antihuman Jak1 monoclonal antibody (Catalog # MAB4260). Tissue was stained using anti-rat HRP-DAB reagents (brown) and counterstained with hematoxylin (blue).
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69
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE JAM-A
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Human
None
Polyclonal
Goat IgG
AF1103
100 µg
SIZE
PRICE $355
Mouse
None
Polyclonal
Goat IgG
AF1077
100 µg
$355
JAM-C
Mouse
None
Polyclonal
Goat IgG
AF1213
100 µg
$355
JNK Pan Specific
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF1387
100 µg
$285
Phospho-JNK (T183/Y185)
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF1205
50 µg
$285
JNK1/JNK2
Human/Mouse/Rat
None
252323
Mouse IgG2A
MAB2076
100 µg
$240
JNK1
Human/Mouse/Rat
None
228601
Mouse IgG1
MAB17761
100 µg
$240
JNK2
Human/Mouse/Rat
None
252320
Mouse IgG2B
MAB1846
100 µg
$240
c-Jun aa 1-331
Human/Mouse
None
320831
Mouse IgG1
MAB2670
100 µg
$240
Kallikrein 3/PSA
Human
None
Polyclonal
Goat IgG
AF1344
100 µg
$355
Kallikrein 5
Human
None
Polyclonal
Goat IgG
AF1108
100 µg
$355
Kallikrein 6/Neurosin
Human
None
Polyclonal
Goat IgG
AF2008
100 µg
$355
Polyclonal
Goat IgG
AF2624
100 µg
$355
Polyclonal
Goat IgG
AF3486
100 µg
$355
None
Polyclonal
Goat IgG
AF1595
100 µg
$355
None
189308
Mouse IgG3
MAB1595
500 µg
$325
Kallikrein 6 in Human Breast Cancer. Kallikrein 6/ Neurosin was detected in paraffin-embedded human breast cancer tissue sections using anti-human Kallikrein 6 affinity-purified polyclonal antibody (Catalog # AF2008). Tissues were stained using anti-goat HRPDAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Kallikrein 7
Human
None
Kallikrein 7 in Normal Human Skin. Kallikrein 7 (KLK7) was detected in paraffin-embedded normal human skin sections using anti-human KLK7 affinitypurified polyclonal antibody (Catalog # AF2624). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Kallikrein 9
Human
None
Kallikrein 9 in Human Ovarian Cancer. Kallikrein 9 (KLK9) was detected in paraffin-embedded human ovarian cancer sections using anti-human KLK9 affinity-purified polyclonal antibody (Catalog # AF3486). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Kallikrein 11
Human
Kallikrein 12
Human
None
Polyclonal
Sheep IgG
AF3095
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF2625
100 µg
$355
Kallikrein 13
Kallikrein 13 in Human Prostate Cancer. Kallikrein 13 (KLK13) was detected in paraffin-embedded human prostate cancer sections using anti-human KLK13 affinity-purified polyclonal antibody (Catalog # AF2625). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
70
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Kallikrein 15 KLF4
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Human
None
Polyclonal
Goat IgG
AF2540
100 µg
SIZE
PRICE $355
Human
None
Polyclonal
Goat IgG
AF3640
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF3158
100 µg
$355
KLF5
Human
None
Polyclonal
Goat IgG
AF3758
100 µg
$355
Klotho
Mouse
None
Polyclonal
Goat IgG
AF1819
100 µg
$355
None
236214
Rat IgG2A
MAB1819
500 µg
$325
None
387301
Mouse IgG1
MAB3895
100 µg
$240
None
387310
Mouse IgG1
MAB38951
100 µg
$240
Klotho in Mouse Kidney. Klotho was detected in a frozen section of mouse kidney tissue using antimouse Klotho affinity-purified polyclonal antibody (Catalog # AF1819). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
KOR
Kremen-1 Kremen-1 Laminin a1
Human Human
None
Polyclonal
Goat IgG
AF2127
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF1647
100 µg
$355
Mouse
Biotin
Polyclonal
Goat IgG
BAF1647
50 µg
$380
Mouse
None
AL-4
Rat IgG2A
MAB4656
100 µg
$240
Laminin a1 in Mouse Decidua. Laminin a1 was detected in frozen sections of mouse E10.5 decidua using anti-mouse laminin a1 monoclonal antibody (Catalog # MAB4656; red). Tissue was counterstained with DAPI (blue).
Laminin a1 N-Terminus Domain VI
Human
None
Polyclonal
Goat IgG
AF4187
100 µg
$355
None
Polyclonal
Goat IgG
AF3837
100 µg
$355
Laminin a4
Mouse
Biotin
Polyclonal
Goat IgG
BAF3837
50 µg
$380
Laminin g1
Human/Rat
None
D18
Mouse IgG2A
MAB2139
100 µg
$240
Laminin S
Human/Rat/Chicken
None
C4
Mouse IgG1
MAB2066
100 µg
$295
Laminin-1
Mouse
None
AL-2
Rat IgG1
MAB2549
100 µg
$240
None
AL-3
Rat IgG1
MAB25491
100 µg
$240
Laminin-5
Human
None
P3H9-2
Mouse IgG1
MAB2144
250 µg
$240
Laminin-5 in Human MCF10A Cells. Laminin-5 was detected in human MCF10A breast epithelial cell line using anti-human Laminin-5 monoclonal antibody (Catalog # MAB2144). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
LAMP1/CD107a Lumenal Domain
Human
None
Polyclonal
Sheep IgG
AF4800
100 µg
$355
Langerin/CD207
Human
None
Polyclonal
Goat IgG
AF2088
100 µg
$355
Lck
Human
None
355713
Mouse IgG2B
MAB3704
100 µg
$240
Human LDL R
Mouse
None
Polyclonal
Goat IgG
AF2148
100 µg
$355
None
Polyclonal
Goat IgG
AF2255
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2255
50 µg
$380
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
71
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE LEDGF Leptin/OB
Leptin R
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Human
None
Polyclonal
Goat IgG
AF3468
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF398
100 µg
$355
Mouse Human Mouse
SIZE
PRICE
None
Polyclonal
Goat IgG
AF498
100 µg
$355
None
Polyclonal
Goat IgG
AF389
100 µg
$355
None
52263
Mouse IgG2B
MAB867
500 µg
$325
None
Polyclonal
Goat IgG
AF497
100 µg
$355
None
Polyclonal
Goat IgG
AF-250-NA
100 µg
$355
None
9824
Mouse IgG2B
MAB250
500 µg
$325
Leptin Receptor in Rat Brain. Leptin Receptor was detected in cryostat tissue sections of rat brain using anti-mouse Leptin Receptor affinity-purified polyclonal antibody (Catalog # AF497). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
LIF
Human Mouse
None
Polyclonal
Total Goat IgG
AB-449-NA
1 mg
$355
LILRC1
Rat
None
Polyclonal
Goat IgG
AF4074
100 µg
$355
LIM1
Human
None
320416
Mouse IgG2A
MAB2725
100 µg
$240
Limitin
Mouse
None
Polyclonal
Goat IgG
AF597
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF597
50 µg
$380
LIN-28
Human
None
Polyclonal
Goat IgG
AF3757
100 µg
$355
LIN-41
Human
None
Polyclonal
Sheep IgG
AF5104
100 µg
$355 $355
LINGO-1
Human
None
Polyclonal
Goat IgG
AF3086
100 µg
LINGO-1 aa 40-556
Human
None
332237
Mouse IgG1
MAB30861
100 µg
$240
None
Polyclonal
Goat IgG
AF3679
100 µg
$355
None
382007
Mouse IgG2A
MAB36791
100 µg
$240
None
Polyclonal
Goat IgG
AF3885
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF3885
50 µg
$380
LINGO-2
Human
Lipin 1
Human
Lipin 3
Mouse
Lipocalin-2/NGAL
Human
LMIR3/CD300LF
Human
LMO2 LRRC4
Human Human
None
Polyclonal
Goat IgG
AF4064
100 µg
$355
None
Polyclonal
Goat IgG
AF1757
100 µg
$355
None
220310
Rat IgG2A
MAB1757
500 µg
$325
None
234903
Rat IgG2B
MAB2774
100 µg
$240
None
Polyclonal
Goat IgG
AF2726
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2726
50 µg
$380
None
Polyclonal
Sheep IgG
AF4995
100 µg
$355
LRRC4 in Human Cerebellum. Leucine rich repeat/ LRR-containing protein 4 (LRRC4) was detected in paraffin-embedded sections of human cerebellum using anti-human LRRC4 affinity-purified polyclonal antibody (Catalog # AF4995). Tissue was stained using anti-sheep HRP-DAB secondary reagents (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
72
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE LRRN1/NLRR-1
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human
None
Polyclonal
Sheep IgG
AF4990
100 µg
$355
Polyclonal
Sheep IgG
AF5589
100 µg
$355
LRRN1 in Mouse Somites. Leucine rich repeat neuronal 1 (LRRN1) was detected in a frozen section from E9.5 mouse somite using anti-human LRRN1 affinitypurified polyclonal antibody (Catalog # AF4990). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
LRRTM2 Lumican
LXRa/NR1H3 Ligand Binding Domain
Lymphotoxin bR/TNFRSF3
Lyn aa 1-67
Lyp
Human/Mouse Human
None
Polyclonal
Goat IgG
AF2846
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF2745
100 µg
$355
None
PPZ0412
Mouse IgG2A
PP-PPZ0412-00
100 µg
$380
Human
Mouse
None
PPZ0412
Mouse IgG2A
PP-PPZ0412-10
100 µg
$380
None
Polyclonal
Goat IgG
AF1008
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1008
50 µg
$380 $355
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF3206
100 µg
Human/Rat
None
338716
Mouse IgG2B
MAB3206
100 µg
$240
None
Polyclonal
Goat IgG
AF3428
100 µg
$355
None
340113
Mouse IgG2B
MAB3428
100 µg
$240
None
Polyclonal
Goat IgG
AF2089
100 µg
$355
Human
Human LYVE-1 Mouse
MAdCAM-1
Mouse
MafF
Human
MAG/Siglec-4a
None
Rat
Biotin
Polyclonal
Goat IgG
BAF2089
50 µg
$380
None
Polyclonal
Goat IgG
AF2125
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2125
50 µg
$380
None
223322
Rat IgG2A
MAB2125
500 µg
$325
None
Polyclonal
Goat IgG
AF993
100 µg
$355
None
Polyclonal
Goat IgG
AF3917
100 µg
$355
None
Polyclonal
Goat IgG
AF538
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF538
50 µg
$380
None
Polyclonal
Goat IgG
AF2567
100 µg
$355
None
290622
Rat IgG2A
MAB2567
100 µg
$240
None
Polyclonal
Goat IgG
AF3044
100 µg
$355
None
Polyclonal
Goat IgG
AF932
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF932
50 µg
$380
None
542808
Mouse IgG2B
MAB828
100 µg
$240
MAG/Siglec-4a in Rat Cerebellum. Myelin-associated Glycoprotein (MAG/Siglec-4a) was detected in cryostat sections of rat cerebellum using anti-rat MAG affinity-purified polyclonal antibody (Catalog # AF538). Tissues were stained using the anti-goat HRP DAB Cell and Tissue Staining Kit (Catalog # CTS008); brown) and counterstained with hematoxylin (blue).
Mash1
Mouse
Matrilin-2
Human
MCAM/CD146
Human
Mcl-1 aa 1-230
Human
M-CSF R
Mouse
None
Polyclonal
Sheep IgG
AF3818
100 µg
$355
Phospho-M-CSF R (Y723)
Human
None
Polyclonal
Rabbit IgG
AF3268
50 µg
$285
MD-1
Human
None
Polyclonal
Goat IgG
AF925
100 µg
$355
MD-2
Human
Biotin
Polyclonal
Sheep IgG
BAF1787
50 µg
$380
MDM2
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF1244
100 µg
$285
MEK1/MEK2
Human/Mouse/Rat
None
239416
Mouse IgG2B
MAB2678
100 µg
$240
Phospho-MEK1/MEK2 (MEK1 S218/S2220, MEK2 S222/S226)
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF2506
100 µg
$285
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
73
R&D SYSTEMS IHC/ICC ANTIBODIES SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
MEK2
MOLECULE
Human/Mouse/Rat
None
300317
Mouse IgG2A
MAB2855
100 µg
$240
MELK
Human
None
Polyclonal
Sheep IgG
AF4820
100 µg
$355
Melusin
Human
None
Polyclonal
Goat IgG
AF2740
100 µg
$355
None
308705
Mouse IgG2B
MAB2740
100 µg
$240
Meprin a Subunit/MEP1A
Human
None
Polyclonal
Goat IgG
AF3220
100 µg
$355
Meprin b Subunit/MEP1B
SIZE
PRICE
Human
None
Polyclonal
Goat IgG
AF2895
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF3300
100 µg
$355
Meprin b Subunit in Mouse Intestine. Meprin b Subunit (MEP1B) was detected in a frozen section of mouse intestine using anti-mouse MEP1B affinitypurified polyclonal antibody (Catalog # AF3300). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
MESDC2
Human
None
Polyclonal
Sheep IgG
AF5577
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF4545
100 µg
$355
MESDC2 in Adult Mouse Testis. Mesoderm development candidate 2 (MESDC2) was detected in section of mouse testis using anti-mouse MESDC2 affinity-purified polyclonal antibody Catalog # AF4545). The tissue was stained using anti-goat NorthernLight-557 secondary antibody (Catalog # NL001, red) and the nuclei were counterstained with DAPI (blue).
Mesothelin Isoform 1, aa 296-580 Meteorin
Human Mouse
None
420404
Rat IgG2A
MAB32651
100 µg
$240
None
Polyclonal
Goat IgG
AF3475
100 µg
$355
None
347505
Rat IgG2B
MAB3475
100 µg
$240
Meteorin in Rat Cortical Stem Cells. Meteorin was detected in 7-day differentiated rat cortical stem cells using anti-mouse Meteorin monoclonal antibody (Catalog # MAB3475). Cells were stained using NorthernLights-557 goat anti-rat IgG secondary antibody (Catalog # NL013; red) and counterstained with DAPI (blue).
MFRP
Human
None
291204
Rat IgG1
MAB19151
100 µg
$240
Mouse
None
Polyclonal
Goat IgG
AF3445
100 µg
$355
mGluR1a C-Terminus
Rat
None
Polyclonal
Rabbit IgG
PPS077
100 µL
$355
mGluR3
Human
None
Polyclonal
Sheep IgG
AF4668
100 µg
$355
mGluR5
Human
None
Polyclonal
Sheep IgG
AF4514
100 µg
$355
None
464823
Mouse IgG2B
MAB45141
100 µg
$240
mGluR5 in Human Brain (Putamen). Metabotropic glutamate receptor 5 (mGluR5) was detected in a paraffin-embedded human brain (putamen) section using anti-human mGluR5 affinity-purified polyclonal antibody (Catalog # AF4514). The tissue was stained using anti-sheep HRP-DAB detection reagents (brown) and counterstained with hematoxylin (blue).
74
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
mGluR5/1a
MOLECULE
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
PPS079
100 µL
$355
mGluR8
Human
None
476410
Mouse IgG2B
MAB5277
100 µg
$240
MIA
Human
None
Polyclonal
Goat IgG
AF2050
100 µg
$355
None
294203
Mouse IgG2B
MAB20501
100 µg
$240
None
Polyclonal
Goat IgG
AF1300
100 µg
$355
MICA
MICL/CLEC12A
Human Human
Biotin
Polyclonal
Goat IgG
BAF1300
50 µg
$380
None
Polyclonal
Goat IgG
AF2946
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF2950
100 µg
$355
Midkine
Human
None
Polyclonal
Goat IgG
AF-258-PB
100 µg
$355
Mineralocorticoid R/NR3C2
Human
None
H3122
Mouse IgG2A
PP-H3122-00
100 µg
$380
Mineralocorticoid R/NR3C2 aa 1-670
Human
None
385707
Mouse IgG2B
MAB4369
100 µg
$240
MIS/AMH
Rat
None
Polyclonal
Goat IgG
AF1446
100 µg
$355
MIS/AMH Propeptide MIS RII
Human
None
Polyclonal
Goat IgG
AF2748
100 µg
$355
Human
None
Polyclonal
Sheep IgG
AF4749
100 µg
$355
Rat
None
Polyclonal
Goat IgG
AF1618
100 µg
$355
MITF
Human
None
Polyclonal
Goat IgG
AF5769
100 µg
$355
MIXL1
Human
None
319919
Mouse IgG2A
MAB2610
100 µg
$240
MKK3
Human/Mouse/Rat
None
275909
Mouse IgG2B
MAB2515
100 µg
$240
MKK4
Human
None
323042
Mouse IgG2B
MAB3390
100 µg
$240
Phospho-MKK4 (S257/T261)
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF2990
100 µg
$285
MKK6
Human/Mouse/Rat
None
304328
Rat IgG2B
MAB1604
100 µg
$240
MKK7
Human
None
Polyclonal
Goat IgG
AF3579
100 µg
$355
None
324302
Mouse IgG2B
MAB3579
100 µg
$240
MLK4a aa 560-570
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF3435
100 µg
$285
Pan MLK-4 in Human Colon Cancer. Mixed lineage Kinase-4 (MLK-4) was detected in paraffin-embedded human colon cancer sections using anti-human/ mouse/rat pan MLK-4 affinity-purified polyclonal antibody (Catalog # AF3435). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). Tissue was stained using antirabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue).
MLX
Human
MMP-1
Human
Pro MMP-1
Human
MMP-2
Human Mouse/Rat
MMP-3
Human
None
Polyclonal
Goat IgG
AF4186
100 µg
$355
None
Polyclonal
Goat IgG
AF901
100 µg
$355
None
36665
Mouse IgG1
MAB901
200 µg
$295
None
36660
Mouse IgG1
MAB900
200 µg
$295
None
Polyclonal
Goat IgG
AF902
100 µg
$355
None
36006
Mouse IgG1
MAB902
200 µg
$295
None
Polyclonal
Goat IgG
AF1488
100 µg
$355
None
Polyclonal
Goat IgG
AF513
100 µg
$355
None
50647
Mouse IgG1
MAB513
500 µg
$325
None
10D6
Mouse IgG1
MAB905
200 µg
$295
MMP-3 in Human Osteosarcoma Cell Line (MG63). Matrix Metalloproteinase-3 (MMP-3) was detected in MG-63 cells using anti-human MMP-3 monoclonal antibody (Catalog # MAB513). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
75
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
MMP-7
Pro MMP-7
MMP-8
SPECIES Human
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Polyclonal
Goat IgG
AF907
100 µg
$355
None
111433
Mouse IgG2B
MAB9071
500 µg
$325 $355
Mouse
None
Polyclonal
Goat IgG
AF2967
100 µg
Human
None
6A4
Mouse IgG1
MAB907
200 µg
$295
None
Polyclonal
Goat IgG
AF908
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF908
None
100608
Mouse IgG2A
MAB9081
None
Polyclonal
Total Goat IgG
None
Polyclonal
Human
Human MMP-9
Mouse
MMP-10
LABEL None
Human
50 µg
$380
500 µg
$325
AB911
1 mg
$355
Goat IgG
AF911
100 µg
$355 $295
None
4H3
Mouse IgG1
MAB911
200 µg
None
36020
Mouse IgG1
MAB936
500 µg
$325
None
Polyclonal
Goat IgG
AF909
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF909
50 µg
$380
None
Polyclonal
Goat IgG
AF910
100 µg
$355
None
110304
Mouse IgG1
MAB910
500 µg
$325
None
117239
Mouse IgG2B
MAB9101
500 µg
$325
None
110316
Mouse IgG1
MAB9102
500 µg
$325
MAB919
500 µg
$325
MMP-12 Catalytic Domain
Human
None
82902
Mouse IgG2B
MMP-12 Hemopexin Domain
Human
None
4D2
Mouse IgG1
MAB917
200 µg
$295
None
Polyclonal
Goat IgG
AF511
100 µg
$355
None
87512
Mouse IgG1
MAB511
500 µg
$325
MMP-13
Human
MMP-13 in Human Ovarian Cancer Tissue. Matrix metalloproteinase-13 (MMP-13) producing cells in human ovarian cancer tissue were detected using anti-human MMP-13 affinity-purified antibody (Catalog # AF511). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and Hematoxylin (blue) counterstain.
Pro MMP-13
Human
None
87518
Mouse IgG2B
MAB913
500 µg
$325
None
Polyclonal
Goat IgG
AF918
100 µg
$355
None
128527
Mouse IgG2B
MAB9181
500 µg
$325
MMP-14
Human
MMP-14 aa 165-175
Human
None
5H2
Mouse IgG1
MAB918
500 µg
$325
MMP-15
Human
None
130522
Mouse IgG1
MAB9161
500 µg
$325
MMP-15 Catalytic Domain
Human
None
130516
Mouse IgG1
MAB916
500 µg
$325 $355
MMP-24/MT5-MMP
Human/Mouse
None
Polyclonal
Total Rabbit IgG
AB924
200 µg
MMP-25/MT6-MMP Hemopexin Domain
Human
None
141825
Mouse IgG1
MAB11421
500 µg
$325
None
Polyclonal
Goat IgG
AF2534
100 µg
$355
None
309210
Rat IgG2A
MAB2534
100 µg
$240 $355
MMR
MOG
Human Human
None
Polyclonal
Goat IgG
AF2395
100 µg
Human/Mouse
Biotin
296829
Rat IgG2B
BAM2439
100 µg
$380
None
Polyclonal
Goat IgG
AF2439
100 µg
$355
Mouse MRP1 MSK1
Biotin
Polyclonal
Goat IgG
BAF2439
50 µg
$380
Human
None
QCRL
Mouse IgG1
MAB19291
100 µg
$240
Human
None
252608
Mouse IgG2B
MAB2518
100 µg
$240
Human/Mouse
None
Polyclonal
Goat IgG
AF2518
100 µg
$355
Phospho-MSK1 (S212)
Human
None
Polyclonal
Rabbit IgG
AF1036
100 µg
$285
Phospho-MSK2 (S196)
Human/Mouse
None
Polyclonal
Rabbit IgG
AF189
100 µg
$285
MUCDHL
Human
None
Polyclonal
Goat IgG
AF3826
100 µg
$355
76
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Musashi-1
SPECIES Human
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF2628
100 µg
$355
None
282613
Mouse IgG2B
MAB2628
100 µg
$240
Musashi-1 in Human Intestine. Musashi-1 was detected in a paraffin-embedded human intestine section using anti-human Musashi-1 affinity-purified polyclonal antibody (Catalog # AF2628). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Mxi1
Human
None
Polyclonal
Goat IgG
AF4185
100 µg
$355
c-Myc aa 66-201
Human
None
Polyclonal
Goat IgG
AF3696
100 µg
$355
None
Polyclonal
Goat IgG
AF2928
100 µg
$355 $240
Human MyD88
Myeloperoxidase/MPO Myeloperoxidase/MPO aa 279-745
None
316603
Rat IgG2A
MAB2928
100 µg
Mouse
None
316902
Rat IgG2A
MAB3109
100 µg
$240
Mouse/Rat
None
Polyclonal
Goat IgG
AF3109
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF3174
100 µg
$355
Human/Mouse
None
Polyclonal
Goat IgG
AF3667
100 µg
$355
Human/Mouse
None
392105
Mouse IgG2B
MAB3174
100 µg
$240
Myeloperoxidase in Human Spleen. Myeloperoxidase (MPO) was detected in paraffin-embedded human spleen tissue sections using anti-human/mouse MPO monoclonal antibody (Catalog # MAB3174) and Antigen Retrieval Reagent - Basic (Catalog # CTS013). The tissue was stained using anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
Myosin Heavy Chain
Human
None
MF20
Mouse IgG2B
MAB4470
100 µg
$240
NAALADase-like 2/NAALADL2
Human
None
Polyclonal
Goat IgG
AF4665
100 µg
$355
None
Polyclonal
Goat IgG
AF1997
50 µg
$355
Nanog
Human Mouse
Biotin
Polyclonal
Goat IgG
BAF1997
50 µg
$395
None
Polyclonal
Goat IgG
AF2729
100 µg
$355
NL1997G
500 µL
$350
Nanog in Human BG01V Embryonic Stem Cells. Nanog was detected in human BG01V embryonic stem cells using anti-human Nanog affinity-purified polyclonal antibody (Catalog # AF1997). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
Nanog NorthernLights-conjugated
Human
NL493
Polyclonal
Goat IgG
NCAM-1/CD56
Human
None
301021
Mouse IgG2B
MAB24081
100 µg
$240
NCAM-L1
Human
None
Polyclonal
Goat IgG
AF277
100 µg
$355
Nectin-3
Human
None
Polyclonal
Goat IgG
AF3064
100 µg
$355
Nectin-4
Human
None
Polyclonal
Goat IgG
AF2659
100 µg
$355
None
337506
Mouse IgG2A
MAB26591
100 µg
$240
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
77
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE NEDD8
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human/Mouse/Rat
None
Polyclonal
Sheep IgG
AF4936
100 µg
$355
Polyclonal
Goat IgG
AF1079
100 µg
$355
NEDD8 in Human Skeletal Muscle. Neural precursor cell-expressed developmentally down-regulated gene 8 (NEDD8) was detected in paraffin-embedded human skeletal muscle sections using anti-human/ mouse/rat NEDD8 affinity-purified polyclonal antibody (Catalog # AF4936). Tissue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
Neogenin Nephrin
Mouse
None
Human
None
Polyclonal
Sheep IgG
AF4269
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF3159
100 µg
$355
Polyclonal
Goat IgG
AF1182
100 µg
$355 $380
Nephrin in Mouse Kidney. Nephrin was detected in frozen sections of mouse kidney using anti-mouse nephrin affinity-purified polyclonal antibody (Catalog # AF3159). Tissue was stained using anti-goat IgG NorthernLights-557-conjugated secondary antibody (Catalog # NL001; red) and counterstained green.
None
Human Neprilysin/CD10
Nestin
Biotin
Polyclonal
Goat IgG
BAF1182
50 µg
Human/Mouse
None
200103
Rat IgG2B
MAB1126
500 µg
$325
Mouse
None
Polyclonal
Goat IgG
AF1126
100 µg
$355
Human
None
196908
Mouse IgG1
MAB1259
100 µg
$240
None
Polyclonal
Goat IgG
AF2736
100 µg
$355
None
307501
Mouse IgG2A
MAB2736
100 µg
$240
$345
Rat
Nestin in Undifferentiated Rat Neural Stem Cells. Nestin was detected in rat cortical stem cells using anti-rat Nestin monoclonal antibody (Catalog # MAB2736). The cells were stained using anti-mouse NorthernLights-557 secondary antibody (Catalog # NL007; red) and the nuclei counterstained with DAPI (blue).
Nestin NorthernLights-conjugated
Human
NL637
196908
Mouse IgG1
NL1259V
500 µL
Rat
NL557
307501
Mouse IgG2A
NL2736R
500 µL
$345
None
Polyclonal
Goat IgG
AF1254
100 µg
$355
Human Netrin-4 Mouse
Biotin
Polyclonal
Goat IgG
BAF1254
50 µg
$380
None
Polyclonal
Goat IgG
AF1132
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1132
50 µg
$380
349724
Rat IgG2A
MAB27441
100 µg
$240
Netrin-4 in Mouse Brain. Netrin-4 was detected in frozen sections of mouse brain (cerebellum) using anti-mouse Netrin-4 affinity-purified polyclonal antibody (Catalog # AF1132). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Netrin-G2a
78
Mouse
None
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
NRG1-a/HRG1-a EGF Domain
MOLECULE
Human
None
Polyclonal
Goat IgG
AF-296-NA
100 µg
PRICE $355
NRG1-b1/HRG1-b1 EGF Domain
Human
None
Polyclonal
Goat IgG
AF-396-NA
100 µg
$355
Neuregulin-3/NRG3 EGF-like Domain
Mouse
None
Polyclonal
Goat IgG
AF753
100 µg
$355
Neurexin 3/NRXN3
Human/Mouse
None
Polyclonal
Sheep IgG
AF5269
100 µg
$355
Neuritin
Human
None
Polyclonal
Goat IgG
AF283
100 µg
$355
Neurocan
Mouse/Rat
NeuroD1
Human
NF-H
Human
None
Polyclonal
Sheep IgG
AF5800
100 µg
$355
None
Polyclonal
Goat IgG
AF2746
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2746
50 µg
$380
None
Polyclonal
Goat IgG
AF3108
100 µg
$355
Neurofilament-H in Human Brain. Neurofilament-H (NF-H) was detected in paraffin-embedded human brain (cerebellum) tissue sections using anti-human NF-H affinity-purified polyclonal antibody (Catalog # AF3108). Tissues were stained using anti-goat HRPDAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
NF-L
Human
None
268014
Mouse IgG2B
MAB2216
100 µg
$240
NF-M
Human
None
Polyclonal
Sheep IgG
AF3029
100 µg
$355
Neurogenin-2
Human/Rat
None
7G4
Mouse IgG2A
MAB3314
100 µg
$240
Human
None
Polyclonal
Sheep IgG
AF5685
100 µg
$355
Mouse/Rat
None
Polyclonal
Goat IgG
AF5665
100 µg
$355
Neuroglycan C/CSPG5
Neuroglycan C in Mouse Cerebellum. Neuroglycan C was detected in a section of adult mouse cerebellum using anti-mouse/rat Neuroglycan affinity-purified polyclonal antibody (Catalog # AF5665). The cells were stained using NorthernLights-557 anti-goat lgG secondary antibody (Catalog # NL001; red) and the nuclei counterstained with DAPI (blue).
Neuronal Pentraxin R
Human
None
Polyclonal
Sheep IgG
AF4414
100 µg
$355
Neurophysin II
Human
None
Polyclonal
Goat IgG
AF009
100 µg
$355
Human
None
Polyclonal
Sheep IgG
AF3870
100 µg
$355
None
Polyclonal
Goat IgG
AF566
100 µg
$355
None
130604
Mouse IgG1
MAB5661
500 µg
$325
Polyclonal
Goat IgG
AF2215
100 µg
$355
Neuropilin-1/BDCA4
Rat
Neuropilin-1 Expression in Mouse Embryonic Spinal Cord. Neuropilin-1 was detected in a cryostat section of mouse embryonic spinal cord (E15) using anti-rat Neuropilin-1 affinity-purified polyclonal antibody (Catalog # AF566). Tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Neuropilin-2
Human
None
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
79
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Neuroplastin Isoform 1
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human
None
Polyclonal
Sheep IgG
AF5174
100 µg
$355
None
Polyclonal
Goat IgG
AF5360
100 µg
$355
None
Polyclonal
Goat IgG
AF1235
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1235
50 µg
$380
None
Polyclonal
Goat IgG
AF387
100 µg
$355
Neuroplastin in Human Cerebellum. Neuroplastin was detected in a paraffin-embedded human cerebellum section using anti-human Neuroplastin affinity-purified polyclonal antibody (Catalog # AF5174). The tissue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
Neuroplastin 65
Human/Mouse
Neurotrimin
Human
Neurturin
Human Human/Mouse
None
Polyclonal
Goat IgG
AF477
100 µg
$355
NF2/Merlin aa 363-578
Human
None
Polyclonal
Goat IgG
AF5616
100 µg
$355
NFκB2 aa 1-447
Human
None
291319
Mouse IgG1
MAB28881
100 µg
$240
NG2/MCSP
Human
None
LHM-2
Mouse IgG1
MAB2585
100 µg
$240
Human
None
Polyclonal
Goat IgG
AF-256-NA
100 µg
$355
Rat
None
Polyclonal
Goat IgG
AF-556-NA
100 µg
$355
Human
None
74902
Mouse IgG1
MAB367
100 µg
$240
None
Polyclonal
Goat IgG
AF1157
100 µg
$355
b-NGF
NGF R/TNFRSF16
Mouse
Nicastrin Isoform 1
Human
Nidogen-1/Entactin
Human
Biotin
Polyclonal
Goat IgG
BAF1157
50 µg
$380
None
Polyclonal
Sheep IgG
AF5378
100 µg
$355
None
Polyclonal
Goat IgG
AF2570
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2570
50 µg
$380
None
302117
Mouse IgG1
MAB2570
100 µg
$240
None
Polyclonal
Goat IgG
AF3385
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF3385
50 µg
$380
AF5056
100 µg
$355
Nidogen-2
Human
Ninjurin-2
Human
None
Polyclonal
Sheep IgG
NKG2D/CD314
Mouse
Biotin
191004
Rat IgG2B
BAM1547
100 µg
$380
None
Polyclonal
Goat IgG
AF1850
100 µg
$355
None
195314
Mouse IgG2B
MAB1850
500 µg
$325
None
Polyclonal
Goat IgG
AF2444
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2444
50 µg
$380
None
259416
Mouse IgG1
MAB2444
100 µg
$240
NKp46/NCR1
Human
NKX2.5
Human
NKX6.1
Human/Mouse
None
Polyclonal
Goat IgG
AF5857
100 µg
$355
NMDA R, NR1 Subunit C1 Splice Variant
Mouse/Rat
None
Polyclonal
Rabbit IgG
PPS080
25 µg
$355
NMDA R, NR1 Subunit C2 Splice Variant
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
PPS082
25 µg
$355
Mouse/Rat
None
Polyclonal
Rabbit IgG
PPS081
25 µg
$355
NMDA R, NR1 Subunit N1 Splice Variant
Mouse/Rat
None
Polyclonal
Rabbit IgG
PPS083
25 µg
$355
NMDA R, NR2A Subunit
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
PPS012
10 µg
$355
NMDA R, NR2B Subunit
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
PPS013
10 µg
$355 $355
NMDA R, NR2C Subunit
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
PPS033
10 µg
N-Me-6,7-diOH-TIQ
Multispecies
None
106814
Mouse IgG2B
MAB167
500 µg
$325
Noggin
Mouse
None
Polyclonal
Goat IgG
AF719
100 µg
$355
Nogo-A aa 2-172
Rat
None
Polyclonal
Goat IgG
AF3098
100 µg
$355
80
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Nogo-A aa 566-748
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human
None
Polyclonal
Sheep IgG
AF3515
100 µg
$355
499825
Mouse IgG3
MAB5766
100 µg
$240
Nogo-A in differentiated rat cortical stem cells. Nogo-A was detected in 7-day differentiated rat cortical stem cells using anti-human Nogo-A affinitypurified polyclonal antibody (Catalog # AF3515). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
Nogo-C
Human
Norrin
Human
None None
Polyclonal
Goat IgG
AF3014
100 µg
$355
None
Polyclonal
Goat IgG
AF950
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF950
50 µg
$380
eNOS aa 1030-1203
Human
iNOS aa 781-798
Human
None
2D2-B2
Mouse IgG1
MAB9502
100 µg
$240
nNOS
Human
None
Polyclonal
Goat IgG
AF2416
100 µg
$355
Notch-1
Rat
None
Polyclonal
Goat IgG
AF1057
100 µg
$355
Notch-1 Intracellular Domain, aa 2428-2556
Human
None
433802
Mouse IgG2A
MAB3647
100 µg
$240
None
Polyclonal
Sheep IgG
AF3647
100 µg
$355
Polyclonal
Goat IgG
AF1190
100 µg
$355
Notch-1 in Human SAOS-2 Cells. Notch-1 was detected in human SAOS-2 osteosarcoma cells using anti-human Notch-1 affinity-purified polyclonal antibody (Catalog # AF3647). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
Notch-2
Rat
None
Notch-2 in Rat Brain. Notch-2 was detected in cryostat sections of rat brain (choroid plexus) using anti-rat Notch-2 affinity-purified polyclonal antibody (Catalog # AF1190). Tissues were stained using antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Notch-2 Intracellular Domain
Human
Notch-3
Mouse
Notch-4 Intracellular Domain
Human
None
Polyclonal
Goat IgG
AF3735
100 µg
$355
None
Polyclonal
Goat IgG
AF1308
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1308
50 µg
$380
None
Polyclonal
Goat IgG
AF3847
100 µg
$355
None
411913
Rat IgG2A
MAB3847
100 µg
$240
None
Polyclonal
Goat IgG
AF1640
100 µg
$355 $380
NOV/CCN3
Human
Biotin
Polyclonal
Goat IgG
BAF1640
50 µg
NRAGE
Human
None
353124
Mouse IgG2B
MAB3835
100 µg
$240
NrCAM Isoform 3
Human
None
Polyclonal
Goat IgG
AF2034
100 µg
$355
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
81
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE NT-3
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human
None
Polyclonal
Goat IgG
AF-267-NA
100 µg
$355
NT-3 in Rat Cerebellum. Neurotrophin-3 (NT-3) was detected in a cryostat section of rat cerebellar Purkinje neurons using anti-human NT-3 affinitypurified polyclonal antibody (Catalog # AF-267-NA). Tissues were stained with AEC substrate (red) and counterstained with hematoxylin (blue).
NT-4 Nucleostemin
Human
None
Polyclonal
Goat IgG
AF-268-NA
100 µg
$355
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF1638
100 µg
$355
Nucleostemin in Human U2OS Cells. Nucleostemin was detected in the nucleoli of the U2OS human osteosarcoma cell line using anti-human/mouse/ rat Nucleostemin affinity-purified polyclonal antibody (Catalog # AF1638). Cells were stained using NorthernLights-557 anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
Numb
Nurr-1/NR4A2
None
Polyclonal
Sheep IgG
AF4338
100 µg
$355
Human
Biotin
Polyclonal
Sheep IgG
BAF4338
50 µg
$380
None
447716
Mouse IgG2A
MAB4338
100 µg
$240
Human
None
N1404
Mouse IgG2A
PP-N1404-00
100 µg
$380
Human/Mouse
None
Polyclonal
Goat IgG
AF2156
100 µg
$355
N-WASP
Human
None
Polyclonal
Goat IgG
AF3854
100 µg
$355
OAS2
Human
None
HLS56/3
Mouse N/A
MAB1925
100 µg
$240
OBCAM
Human
None
Polyclonal
Goat IgG
AF2777
100 µg
$355
None
Polyclonal
Goat IgG
AF1759
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1759
50 µg
$380
None
240408
Rat IgG2B
MAB1759
100 µg
$240
Oct-3/4
Human Human/Mouse
Oct-3/4 and Alkaline Phosphatase in Human BG01V Cells. Oct-3/4 was detected in BG01V embryonic stem cells using anti-human Oct-3/4 affinity-purified polyclonal antibody (Catalog # AF1759). Alkaline phosphatase (ALPL) was detected using anti-human/mouse/rat ALPL mono clonal antibody (Catalog # MAB1448). Cells were stained for Oct-3/4 using NorthernLights-637 donkey antigoat IgG secondary antibody (Catalog # NL002; red) and stained for ALPL using NorthernLights-557 donkey anti-mouse IgG secondary antibody (pseudo-stained green; Catalog # NL007).
Oct-3/4 NorthernLights-conjugated
Human/Mouse
Olig1
Human
Olig 1, 2, 3
Human
82
NL493
240408
Rat IgG2B
NL1759G
500 µL
$345
None
Polyclonal
Goat IgG
AF2417
100 µg
$355
None
257219
Mouse IgG2B
MAB2417
100 µg
$240
None
257224
Mouse IgG2A
MAB2230
100 µg
$295
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Olig2
SPECIES Human
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF2418
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2418
50 µg
$380
None
Polyclonal
Goat IgG
AF2356
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2356
50 µg
$380
None
257934
Mouse IgG1
MAB2456
100 µg
$240
Olig2 and Oligodendrocyte Marker O4 in Rat Cortical Stem Cells (RCSC). Olig2 and O4 were detected in 7 day differentiated RCSC using anti-human Olig2 affinity-purified polyclonal antibody (Catalog # AF2418) and anti-human/mouse/rat/chicken O4 monoclonal antibody (Catalog # MAB1326). Cells were stained for Olig2 using NorthernLights-637 anti-goat IgG secondary antibody (Catalog # NL002; red) and for O4 using an anti-mouse IgM Secondary antibody (green).
Olig3
Human Human/Mouse
Oligodendrocyte Marker O1
Human/Mouse/Rat/Chicken
None
O1
Mouse IgM
MAB1327
50 µg
$200
Oligodendrocyte Marker O4
Human/Mouse/Rat/Chicken
None
O4
Mouse IgM
MAB1326
50 µg
$2040
NL637
O4
Mouse IgM
NL1326V
500 µL
$345
Biotin
97505
Mouse IgG1
BAM763
250 µg
$380
Oligodendrocyte Marker O4 in Rat Cortical Stem Cells (RCSC). Oligodendrocyte marker O4 (O4) was detected in 7-day differentiated RCSC using anti-human/mouse/rat/chicken O4 monoclonal antibody (Catalog # MAB1326). Cells were stained using NorthernLights-557 donkey anti-mouse IgG secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
Oligodendrocyte Marker O4 NorthernLights-conjugated
Human/Mouse/Rat/Chicken
Orexin A
Human/Mouse/Rat
Orexin B
Human
None
97505
Mouse IgG1
MAB763
500 µg
$325
None
145202
Mouse IgG1
MAB734
500 µg
$325
$355
Orexin B, p38a, & Integrin b1 in Mouse Brain Stem. Orexin B, p38a, and Integrin b 1 were detected in frozen sections of mouse brain stem using antihuman Orexin B monoclonal antibody (Catalog # MAB734), anti-human/mouse/rat p38a affinity-purified polyclonal antibody (Catalog # AF8691), and anti-mouse Integrin b1 affinity-purified polyclonal antibody (Catalog # AF2405). Tissue was stained with NorthernLights (NL) secondary polyclonal antibodies: NL-493 anti-rabbit IgG (Catalog # NL006; green), NL-557 anti-mouse IgG (Catalog # NL007; red), and NL-637 anti-goat IgG (Catalog # NL002; blue).
Oncostatin M/OSM
OSM Rb
Mouse
None
Polyclonal
Goat IgG
AF-495-NA
100 µg
Human
None
469221
Mouse IgG1
MAB4389
100 µg
$240
None
Polyclonal
Goat IgG
AF662
100 µg
$355
Biotin
118125
Rat IgG2A
BAM662
100 µg
$240
None
118125
Rat IgG2A
MAB662
100 µg
$240
Mouse
Osteoactivin/GPNMB
Human
None
303822
Mouse IgG2B
MAB25501
100 µg
$240
Osteocalcin
Human
None
190125
Mouse IgG1
MAB1419
100 µg
$240
None
Polyclonal
Goat IgG
AF1433
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1433
50 µg
$380
None
190312
Mouse IgG2B
MAB1433
100 µg
$240
Osteopontin/OPN
Human
Mouse
Osteoprotegerin/TNFRSF11B
Human Mouse
None
Polyclonal
Goat IgG
AF808
100 µg
$355
None
Polyclonal
Goat IgG
AF805
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF805
50 µg
$380
Biotin
Polyclonal
Goat IgG
BAF459
50 µg
$380
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
83
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF1979
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1979
50 µg
$380
Otx2
Human
None
246826
Mouse IgG2B
MAB1979
100 µg
$240
OV-6
Human/Rat
None
OV-6
Mouse IgG1
MAB2020
100 µg
$295
Human
None
159403
Mouse IgG1
MAB10541
500 µg
$325
OX40 Ligand/TNFSF4
p21/CIP1/CDKN1A
Mouse
Human
None
Polyclonal
Goat IgG
AF1236
100 µg
$355
None
Polyclonal
Goat IgG
AF1047
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1047
50 µg
$380
None
195720
Mouse IgG2A
MAB1047
200 µg
$240
CTS2256
p21 in Human Breast Cancer. p21 was detected in paraffin-embedded human breast cancer tissue sections using anti-human p21 affinity-purified polyclonal antibody (Catalog # AF1047). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with haematoxylin (blue).
p27/Kip1 Phospho-p38 MAP Kinase (T180/Y182)
Human
None
Polyclonal
50 Tests
$425
Human/Mouse/Rat
None
341116
Rat IgG2A
MAB22561
100 µg
$240
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF869
100 µg
$285
None
Polyclonal
Rabbit IgG
AF8691
100 µg
$285
None
142102
Mouse IgG2B
MAB869
100 µg
$240
Phosphorylated p38 in Embryonic Mouse Eye. Phosphorylated p38 was detected in a frozen section of E15 mouse eye cup using anti-phospho-p38 (T180/Y182) affinity-purified polyclonal antibody (Catalog # AF869). The tissue was stained using antirabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue).
p38a
Human/Mouse/Rat
p38b
Human
None
280071
Mouse IgG2B
MAB3274
100 µg
$240
p38g
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF1347
100 µg
$285
p38d
Human
None
Polyclonal
Rabbit IgG
AF1519
100 µg
$285
Phospho-p53 (S15)
Human
None
Polyclonal
Rabbit IgG
AF1043
100 µg
$285
None
261366
Mouse IgG1
MAB18391
100 µg
$240
Phospho-p53 (S46)
Human
None
Polyclonal
Rabbit IgG
AF1489
100 µg
$285
None
Polyclonal
Goat IgG
AF1916
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1916
50 µg
$380
None
241631
Mouse IgG2B
MAB1916
100 µg
$240
None
Polyclonal
Rabbit IgG
AF8962
100 µg
$285
None
Polyclonal
Goat IgG
AF8964
100 µg
$355
None
215247
Mouse IgG1
MAB8962
100 µg
$240
None
Polyclonal
Goat IgG
AF896
100 µg
$285
p63/TP73L
p70 S6 Kinase
Phospho-p70 S6 Kinase (T229)
84
Human
Human/Mouse/Rat
Human
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE PAK7
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human/Rat
None
444703
Mouse IgG2B
MAB4696
100 µg
$240
Polyclonal
Goat IgG
AF3995
100 µg
$355
100 µg
$355
PAK7 in Human Cerebellum. p21-activated kinase 7 (PAK7) was detected in a paraffin-embedded human cerebellum section using anti-human PAK7 monoclonal antibody (Catalog # MAB4696). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
Park7/DJ-1
Human
None
Parkin
Human
None
Polyclonal
Goat IgG
AF1438
PARP
Multi-species
None
C2-10
Mouse IgG1
4338-MC-50
Parvalbumin a
Human/Mouse/Rat
None
Polyclonal
Sheep IgG
413220
Polyclonal
50 µg
$195
AF5058
100 µg
$355
Rat IgG2A
MAB41051
100 µg
$240
Goat IgG
AF4078
100 µg
$355 $355
Parvalbumin a in Human Cortex. Parvalbumin a was detected in paraffin-embedded sections of human cortex using anti-human/mouse/rat Parvalbumin a monoclonal antibody (Catalog # AF5058). Tissue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
Patched (First Extracellular Loop)
Mouse
None
Patched in Embryonic Mouse Spinal Cord. Patched was detected in ventral E13.5 mouse spinal cord using anti-mouse Patched monoclonal antibody (Catalog # MAB41051; red). Tissue was counterstained with DAPI (blue).
Patched 2 (First Extracellular Loop)
None
Human
None
Polyclonal
Goat IgG
AF2457
100 µg
Human/Mouse
None
274212
Mouse IgG2A
MAB2457
100 µg
$240
Pax4
Human
None
Polyclonal
Goat IgG
AF2614
100 µg
$355
Pax5
Human
Pax3
Pax6
Mouse/Rat/Chicken
Pax7
Human/Mouse/Rat/Chicken
PBR
PD-1
PD-ECGF
PDGF-AA
PDGF-C
Human
None
Polyclonal
Goat IgG
AF3487
100 µg
$355
Biotin
PAX6
Mouse IgG1
BAM1260
100 µg
$260
None
PAX6
Mouse IgG1
MAB1260
100 µg
$240
None
PAX7
Mouse IgG1
MAB1675
100 µg
$240
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
6361-PC-100
100 µL
$310
Mouse/Rat
None
Polyclonal
Rabbit IgG
6362-PC-100
100 µL
$310
Human
None
Polyclonal
Goat IgG
AF1086
100 µg
$355
None
Polyclonal
Goat IgG
AF1021
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1021
50 µg
$380
None
Polyclonal
Goat IgG
AF1143
100 µg
$355
Mouse
Human
Human
Biotin
Polyclonal
Goat IgG
BAF1143
50 µg
$380
None
Polyclonal
Total Goat IgG
AB-221-NA
1 mg
$355
None
Polyclonal
Goat IgG
AF-221-NA
100 µg
$355
Rat
None
Polyclonal
Goat IgG
AF1055
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF1560
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF1447
100 µg
$355
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
85
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE PDGF-D
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Human
None
Polyclonal
Goat IgG
AF1159
100 µg
$355
None
Polyclonal
Goat IgG
AF-307-NA
100 µg
$355
None
35248
Mouse IgG1
MAB322
500 µg
$325
None
Polyclonal
Goat IgG
AF1062
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1062
50 µg
$380
Human PDGF Ra Mouse
SIZE
PRICE
Phospho-PDGF Ra (Y742)
Human
None
Polyclonal
Rabbit IgG
AF2114
50 µg
$285
Phospho-PDGF Ra (Y762)
Human
None
Polyclonal
Rabbit IgG
AF21141
50 µg
$285
None
Polyclonal
Goat IgG
AF385
100 µg
$355
None
PR7212
Mouse IgG1
MAB1263
250 µg
$230
None
Polyclonal
Goat IgG
AF1042
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1042
50 µg
$380
None
Polyclonal
Goat IgG
AF1224
100 µg
$355
None
176611
Mouse IgG2B
MAB1224
500 µg
$325
None
Polyclonal
Goat IgG
AF1022
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1022
50 µg
$380
None
Polyclonal
Goat IgG
AF2419
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2419
50 µg
$380
None
267712
Mouse IgG2B
MAB2419
100 µg
$240
Human PDGF Rb Mouse
PDGF Rb in Mouse Embryo. Platelet-derived Growth Factor Receptor b (PDGF Rb) was detected in a cryostat tissue section of E13 mouse spinal cord using anti-mouse PDGF Rb affinity-purified polyclonal antibody (Catalog # AF1042). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Human PD-L2 Mouse
PDX-1/IPF1
Human Human/Mouse
PDZK1
Human/Mouse
None
Polyclonal
Sheep IgG
AF4997
100 µg
$355
PEDF R
Human/Mouse/Rat
None
Polyclonal
Sheep IgG
AF5365
100 µg
$355
PER1 aa 159-365
Human
None
Polyclonal
Goat IgG
AF4565
100 µg
$355
Periostin/OSF-2
Mouse
None
345613
Rat IgG2A
MAB3548
100 µg
$240
Periostin/OSF-2 Isoform 2
Mouse
None
Polyclonal
Goat IgG
AF2955
100 µg
$355
Persephin
Human
None
275508
Mouse IgG2B
MAB2388
100 µg
$240
PIBF1
Human
None
Polyclonal
Sheep IgG
AF5559
100 µg
$355
pIgR
Mouse
None
Polyclonal
Goat IgG
AF2800
100 µg
$355
PIN
Human
None
Polyclonal
Goat IgG
AF877
100 µg
$355
Pin1 aa 2-163
Human/Mouse
None
257417
Mouse IgG2B
MAB2294
100 µg
$240
PINK1
Human
None
420920
Rat IgG2A
MAB4357
100 µg
$240
PKCb1
Human/Rat
None
Polyclonal
Goat IgG
AF3759
100 µg
$355
PKCι/λ PKC ι/λ/ζ Cross-reactive, aa 446-587
Human/Mouse/Rat
None
Polyclonal
Sheep IgG
AF4465
100 µg
$355
PKR
Human
None
HL71/10
Mouse IgG1
MAB1980
100 µg
$295
Plasminogen Kringle 5
Mouse
None
Polyclonal
Goat IgG
AF742
100 µg
$355
PLC-g2 aa 400-600
Human/Mouse
None
346404
Mouse IgG1
MAB3716
100 µg
$240
Plexin A1
Mouse
None
408305
Rat IgG2A
MAB4309
100 µg
$240
86
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Plexin A3
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Mouse
None
Polyclonal
Goat IgG
AF4075
100 µg
$355
None
Polyclonal
Goat IgG
AF3749
100 µg
$355
None
Polyclonal
Sheep IgG
AF5329
100 µg
$355
None
537237
Mouse IgG2A
MAB5329
100 µg
$240
Plexin A3 Expression in Embryonic Spinal Cord. Plexin A3 was detected in mouse embryonic (E13.5) spinal cord using anti-mouse Plexin A3 affinity-purified polyclonal antibody (Catalog # AF4075). Cells were stained using a NorthernLights-557 secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
Plexin B1
Human
Plexin B2
Human
Plexin B2 in Embryonic Mouse Choroid Plexus. Plexin B2 was detected in a section of embryonic (E13.5) mouse choroid plexus using anti-human Plexin B2 affinity-purified polyclonal antibody (Catalog # AF5329). The tissue was stained using antisheep NorthernLights-557 secondary antibody (Catalog # NL010; red) and the nuclei counterstained with DAPI (blue).
PNR/NR2E3
Human
Human Podocalyxin Mouse
Plexin B2 in Human Colon Cancer. Plexin B2 was detected in paraffin-embedded human colon cancer sections using anti-human Plexin B2 affinity-purified polyclonal antibody (Catalog # AF5329). The tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown). The tissue was counterstained with hematoxylin (blue).
None
H7223
Mouse IgG2A
PP-H7223-00
100 µg
$380
None
Polyclonal
Goat IgG
AF1658
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1658
50 µg
$380
None
222328
Mouse IgG2A
MAB1658
100 µg
$240
None
Polyclonal
Goat IgG
AF1556
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1556
50 µg
$380
None
192703
Rat IgG2B
MAB1556
100 µg
$240
Podocalyxin in Human Kidney. Podocalyxin was detected in a paraffin-embedded human kidney tissue section using anti-human Podocalyxin affinity-purified polyclonal antibody (Catalog # AF1658). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Podocalyxin NorthernLights-conjugated Podocan
Podoplanin Potassium Channel Kv2.2
Podocalyxin in Human Embryonic Stem Cells (BGO1V). Podocalyxin was detected in the BGO1V human embryonic stem cell line cultured on mouse embryonic fibroblasts using anti-human Podocalyxin monoclonal antibody (Catalog # MAB1658). The cells were stained with anti-mouse NorthernLights-557 secondary antibody (Catalog # NL007; red) and counterstained with DAPI (blue).
Human
NL557
222328
Mouse IgG2A
NL1658R
500 µL
Human
None
390117
Mouse IgG2B
MAB42201
100 µg
$345 $240
Mouse
None
Polyclonal
Goat IgG
AF3104
100 µg
$355
Human
None
Polyclonal
Sheep IgG
AF3670
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF3244
100 µg
$355
Rat/Xenopus
None
Polyclonal
Rabbit IgG
PPS015
100 µL
$355
Phospho-Potassium Channel Kv3.1 (S503)
Mouse/Rat
None
Polyclonal
Rabbit IgG
PPS016
100 µL
$355
PP2A Catalytic Subunit, aa 209-309
Human
None
234532
Rat IgG2B
MAB16531
100 µg
$240
PP2Cg/PPM1G
Human
None
528322
Mouse IgG1
MAB5595
100 µg
$240
PPARg/NR1C3
Human
None
A3409A
Mouse IgG2A
PP-A3409A-00
100 µg
$380
None
Polyclonal
Goat IgG
AF166
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF166
50 µg
$380
None
Polyclonal
Goat IgG
AF149
100 µg
$355
None
121121
Mouse IgG2B
MAB149
500 µg
$325
Biotin
Polyclonal
Goat IgG
BAF197
50 µg
$380
Presenilin-1 C-Terminal Fragment, aa 298-407
Human
Presenilin-1 N-Terminal Fragment, aa 1- 80
Human
Presenilin-2 C-Terminal Fragment, aa 322-388
Human
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87
R&D SYSTEMS IHC/ICC ANTIBODIES SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Presenilin-2 N-Terminal Fragment, aa 1- 80
MOLECULE
Human
None
Polyclonal
Goat IgG
AF153
100 µg
$355
PRL/PTP4A Pan Specific
Human
None
334407
Mouse IgG2B
MAB32191
100 µg
$240
None
A9621A
Mouse IgG2A
PP-A9621A-00
100 µg
$380
None
1154
Mouse IgG1
PPS017
100 µL
$355
Human/Mouse/Rat/Primate
None
608
Mouse IgG1
PPS018
100 µL
$355
Human
None
H5344
Mouse IgG2A
PP-H5344-00
100 µg
$380
Progesterone R/NR3C3
Progesterone R/NR3C3 B
Human
Progesterone Receptor-B/NR3C3 in Human Endometrium. Progesterone Receptor isoform B was detected in paraffin-embedded human endometrial epithelial cells using anti-human Progesterone Receptor isoform B monoclonal antibody (Catalog # PPH5344-00). Tissues were stained with a 0.01% DAB solution (brown) and counterstained with hematoxylin (blue).
Human Progranulin
Mouse
Prohibitin
Human
Prolactin
Mouse
Prolactin R Prominin 2 Prostasin/Prss8
Human
None
Polyclonal
Goat IgG
AF2420
100 µg
$355
None
Polyclonal
Sheep IgG
AF2557
100 µg
$355
None
333731
Rat IgG2A
MAB25571
100 µg
$240
None
Polyclonal
Goat IgG
AF3470
100 µg
$355
None
Polyclonal
Goat IgG
AF1445
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1445
50 µg
$380
None
Polyclonal
Goat IgG
AF1167
100 µg
$355
Rat
None
Polyclonal
Goat IgG
AF1112
100 µg
$355
Human
None
244029
Mouse IgG2B
MAB2024
100 µg
$295
Human
None
Polyclonal
Sheep IgG
AF4599
100 µg
$355
Polyclonal
Sheep IgG
AF5064
100 µg
$355
Polyclonal
Goat IgG
AF5899
100 µg
$355
Prostasin in Human Prostate Cancer. Prostasin was detected in a paraffin-embedded human prostate cancer section using anti-human Prostasin affinity-purified polyclonal antibody (Catalog # AF4599). The tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). The section was stained using the antisheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
Protocadherin a1
Human
None
Protocadherin a1 in Mouse Cerebellum. Proto cadherin a1 was detected in a section of adult mouse cerebellum using anti-mouse affinity-purified polyclonal antibody (Catalog # AF5064). The tissue was stained using anti-sheep NorthernLights-557 secondary antibody (Catalog # NL010; red) and the nuclei counterstained with DAPI (blue).
Protocadherin-1
88
Human
None
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Protogenin
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Mouse
None
Polyclonal
Sheep IgG
AF4919
100 µg
$355
Polyclonal
Goat IgG
AF2727
100 µg
$355 $355
Protogenin in Embryonic Neural Tube. Protogenin (PRTG) was detected in a section of E9 mouse neural tube using anti-mouse PRTG affinity-purified polyclonal antibody (Catalog # AF4919). The tissue was stained using NorthernLights-557 anti-sheep polyclonal antibody (Catalog # NL101; red) and counterstained with DAPI (blue).
Prox1
Human
None
PSMA/FOLH1/NAALADase I
Human
None
Polyclonal
Sheep IgG
AF4234
100 µg
PTEN
Human/Mouse/Rat
None
217702
Mouse IgG1
MAB847
100 µg
$240
PTEN aa 385-403
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF847
100 µg
$285
PTH1 R
Human
None
Polyclonal
Sheep IgG
AF5709
100 µg
$355
PTP1B
Human
None
Polyclonal
Rabbit IgG
AF1366
100 µg
$285
PTP b/ζ aa 302-754
Human
None
279245
Rat IgG2A
MAB26881
100 µg
$240
PTP-MEG2
Human/Mouse/Rat
None
291835
Mouse IgG2B
MAB2668
100 µg
$240
PTPRg aa 111-541
Human
None
Polyclonal
Goat IgG
AF4608
100 µg
$355
Polyclonal
Goat IgG
AF3430
100 µg
$355
PTPRg in Human Kidney. Protein Tyrosine Phosphatase, Receptor type g (PTPRg) was detected in paraffin-embedded normal human kidney tissue sections using anti-human PTPRg affinity-purified polyclonal antibody (Catalog # AF4608). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
PTPRσ aa 30-1260
Human
None
RACK1
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF3434
100 µg
$355
Rae-1g
Mouse
None
Polyclonal
Goat IgG
AF1136
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF1145
100 µg
$355
RAGE
Mouse
None
Polyclonal
Goat IgG
AF1179
100 µg
$355
Mouse/Rat
None
175410
Rat IgG2A
MAB1179
500 µg
$325
80707
Mouse IgG2A
MAB6831
500 µg
$325
RAGE Expression in Human Alzheimer’s Brain. Receptor for Advanced Glycation End products (RAGE) was detected in paraffin-embedded Alzheimer’s disease human cerebellum sections using anti-human RAGE affinity-purified polyclonal antibody (Catalog # AF1145). Tissue was stained using the anti-goat HRPDAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
RANK/TNFRSF11A
Human
None
Mouse
None
Polyclonal
Goat IgG
AF692
100 µg
$355
Rap1A/B
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF3767
100 µg
$355
RARRES1
Mouse
None
Polyclonal
Goat IgG
AF4657
100 µg
$355
Reelin
Mouse
None
Polyclonal
Goat IgG
AF3820
100 µg
$355
Reg II
Mouse
None
Polyclonal
Goat IgG
AF2035
100 µg
$355
Reg 2/PAP
Rat
None
293523
Mouse IgG1
MAB1996
100 µg
$240
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89
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Reg IIIb
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Mouse
None
Polyclonal
Sheep IgG
AF5110
100 µg
$355
518818
Rat IgG2A
MAB5678
100 µg
$240
Reg IIIb in Mouse Small Intestine. Reg IIIb was detected in adult mouse small intestine sections using anti-mouse Reg IIIb affinity-purified polyclonal antibody (Catalog # AF5110). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
Reg IIId
Mouse
None
Reg IV
Human
None
Polyclonal
Goat IgG
AF1379
100 µg
$355
RelB aa 382-579
Human
None
315206
Mouse IgG2B
MAB2698
100 µg
$240
321422
Rat IgG2A
MAB4606
50 µg
$240
RelB in Human Lymphoma. RelB was detected in a paraffin-embedded human lymphoma section using anti-human RelB monoclonal antibody (Catalog # MAB2698). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
c-Rel c-Rel aa 1-588
Human
None
Human/Mouse
None
Polyclonal
Goat IgG
AF2699
100 µg
$355
Mouse
Biotin
290512
Rat IgG2A
BAM2699
100 µg
$380
c-Rel in Mouse Splenocytes. c-Rel was detected in mouse splenocytes using anti-mouse c-Rel biotinylated monoclonal antibody (Catalog # BAM2699).Cells were stained using NorthernLights Streptavidin-557 (Catalog # NL999; red) and counterstained with DAPI (blue)..
Renin
Human
None
Polyclonal
Sheep IgG
AF4090
100 µg
$355
Renin 1
Mouse
None
Polyclonal
Goat IgG
AF4277
100 µg
$355
Renin 1 Propeptide
Mouse
None
434703
Rat IgG2A
MAB42771
100 µg
$240
Renin Propeptide
Human
None
411507
Mouse IgG1
MAB4447
100 µg
$240
Resistin
Mouse
None
Polyclonal
Goat IgG
AF1069
100 µg
$355
Ret REVERBb/NR1D2
Rex-1
Human
None
Polyclonal
Goat IgG
AF1485
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF482
100 µg
$355
Human
Human
None
H2729
Mouse IgG3
PP-H2729-00
100 µg
$380
None
Polyclonal
Sheep IgG
AF3598
100 µg
$355
Biotin
Polyclonal
Sheep IgG
BAF3598
50 µg
$380
None
373722
Rat IgG2A
MAB3598
100 µg
$240
Rex-1 in Human NTera2 Cells. Rex-1 was detected in human NTera2 embryonic carcinoma cell line using antihuman monoclonal antibody (Catalog # MAB3598). Cells were stained using NorthernLights-557 goat antirat IgG secondary antibody (Catalog # NL013; red) and counterstained with DAPI (blue).
90
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
Repulsive Guidance Molecule A/RGM-A
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Human
None
Polyclonal
Goat IgG
AF2459
100 µg
$355
None
Polyclonal
Goat IgG
AF2458
100 µg
$355
Mouse
RGM-B
SIZE
PRICE
Biotin
Polyclonal
Goat IgG
BAF2458
50 µg
$380
Human/Mouse
None
Polyclonal
Sheep IgG
AF3597
100 µg
$355
Mouse
Biotin
Polyclonal
Sheep IgG
BAF3597
50 µg
$380
RGM-B in Mouse Brain. Repulsive Guidance Molecule B (RGM-B) was detected in a cryostat section of mouse brain (trigeminal ganglia) using anti-mouse RGM-B affinity-purified polyclonal antibody (Catalog # AF3597). Tissues were stained using anti-sheep HRP-DAB detection (brown) and counterstained with hematoxylin (blue).
Rheb
Human/Mouse/Rat
None
344912
Mouse IgG2B
MAB3426
100 µg
$240
Phospho-Ribosomal Protein S6 (S235/S236)
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF3918
100 µg
$285
None
Polyclonal
Goat IgG
AF1749
100 µg
$355
None
Polyclonal
Goat IgG
AF3147
100 µg
$355
None
356001
Mouse IgG1
MAB3147
100 µg
$240
None
Polyclonal
Goat IgG
AF3076
100 µg
$355 $240
Phospho-RPS6 in Human Colon Cancer. PhosphoRibosomal Protein S6 (S235/S236) was detected in paraffin-embedded human colon cancer tissue sections using anti-human/mouse/rat affinity-purified phospho-RPS6 (S235/S236) affinity-purified polyclonal antibody (Catalog # AF3918). The tissue was stained using anti-rabbit HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS005; brown) and counterstained with hematoxylin (blue).
ROBO1
Rat
ROBO2
Human
ROBO3
Human
None
327111
Mouse IgG2B
MAB3076
100 µg
ROR/NR1F1-3 Pan Specific
Human
None
H3925
Mouse IgG2A
PP-H3925-00
100 µg
$380
Phospho-RSK (S380) Pan Specific
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF889
100 µg
$285
Phospho-RSK1/RSK2 (RSK1 S221, RSK2 S227)
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF892
100 µg
$285
RSK1
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF992
100 µg
$285
RSK2
Human/Mouse/Rat
None
Polyclonal
Rabbit IgG
AF1518
50 µg
$285
Phospho-RSK3 (S218) RUNX2/CBFA1
Human/Mouse
None
Polyclonal
Rabbit IgG
AF893
100 µg
$285
Human
None
Polyclonal
Goat IgG
AF2006
100 µg
$355
Human/Mouse
None
232902
Rat IgG2B
MAB2006
100 µg
$240
RUNX3/CBFA3 aa 186-415
Human
None
Polyclonal
Goat IgG
AF3765
100 µg
$355
RXRa/NR2B1
Human
None
K8508
Mouse IgG2A
PP-K8508-00
100 µg
$380
None
K8508
Mouse IgG2A
PP-K8508-10
100 µg
$380
Ryk
Mouse
None
Polyclonal
Sheep IgG
AF4649
100 µg
$355
S100A2
Human
None
Polyclonal
Goat IgG
AF4870
100 µg
$355
S100A4
Mouse
None
438709
Rat IgG2B
MAB4138
100 µg
$240
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91
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
S100A8
SPECIES
Mouse
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF3059
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF3059
50 µg
$380
None
335806
Rat IgG2B
MAB3059
100 µg
$240
S100A8 in Mouse NIH-3T3 Cells. S100A8 was detected in mouse NIH-3T3 embryonic fibroblast cell line using anti-mouse S100A8 monoclonal antibody (Catalog # MAB3059; red). Cells were counterstained with DAPI (blue).
S100A9
Human
None
474315
Mouse IgG2B
MAB5578
100 µg
$240
Mouse
None
Polyclonal
Goat IgG
AF2065
100 µg
$355
S100A9 in Mouse XB2 Cells. S100A9 was detected in mouse XB2 teratoma cell line using anti-mouse S100A9 affinity-purified polyclonal antibody (Catalog # AF2065). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
Human S100A10
Mouse
None
Polyclonal
Goat IgG
AF1698
100 µg
$355
None
Polyclonal
Goat IgG
AF2377
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2377
50 µg
$380
None
344015
Rat IgG2A
MAB2377
100 µg
$240
S100A11
Human
None
Polyclonal
Goat IgG
AF4874
100 µg
$355
S100A13
Mouse
None
Polyclonal
Goat IgG
AF4328
100 µg
$355
Polyclonal
Sheep IgG
AF5180
100 µg
$355
Polyclonal
Goat IgG
AF1820
100 µg
$355
S100A13 in Embryonic Mouse Cartilage. S100A13 was detected in a cryostat section of developing embryonic mouse (E13) cartilage using anti-mouse S100A13 affinity-purified polyclonal antibody (Catalog # AF4328). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
S100A16
Human
None
S100A16 in Human Brain Cortex. S100A16 was detected in a paraffin-embedded human brain cortex section using anti-human S100A16 affinity-purified polyclonal antibody (Catalog # AF5180). The tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013). The section was stained using the anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
S100B
92
Human
None
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE S100P
SPECIES Human
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF2957
100 µg
$355
None
357517
Mouse IgG1
MAB2957
100 µg
$240
S100P in Human Liver. S100P was detected in paraffin-embedded human liver sections using antihuman S100P affinity-purified polyclonal antibody (Catalog # AF2957). Tissue was stained using antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
SALL1 aa 258-499
Human
None
K9814
Mouse IgG2A
PP-K9814-00
100 µg
$380
d-Sarcoglycan
Human
None
Polyclonal
Goat IgG
AF2778
100 µg
$355
None
Polyclonal
Goat IgG
AF1226
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1226
50 µg
$380
Biotin
177228
Rat IgG2A
BAM1226
250 µg
$380
None
177228
Rat IgG2A
MAB1226
500 µg
$325
Mouse
None
462002
Rat IgG2A
MAB4754
100 µg
$240
Canine
None
Polyclonal
Goat IgG
AF2278
100 µg
$355
Feline
None
Polyclonal
Goat IgG
AF2268
100 µg
$355
Sca-1/Ly6
SCARA5 SCF/c-kit Ligand Phospho-SCF R/c-kit (Y730)
SCF R/c-kit
Mouse
Human
None
Polyclonal
Rabbit IgG
AF3527
50 µg
$285
Human
None
Polyclonal
Goat IgG
AF332
100 µg
$355
None
Polyclonal
Goat IgG
AF1356
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1356
50 µg
$380
Biotin
180627
Rat IgG2A
BAM1356
250 µg
$380
None
180627
Rat IgG2A
MAB1356
500 µg
$325
50 µg
$285
Mouse
SCF R in Mouse Cerebellum. Stem Cell Factor Receptor (SCF R) was detected in frozen sections of mouse cerebellum using anti-mouse SCF R monoclonal antibody (Catalog # MAB1356). Tissue was stained using anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).
SCF R/c-kit aa 716-726
Human
None
Polyclonal
Rabbit IgG
AF3267
SCGF/CLEC11a
Human
None
239029
Mouse IgG2A
MAB1904
500 µg
$325
SCL/Tal1
Human
None
Polyclonal
Goat IgG
AF3360
100 µg
$355
SCL/Tal1
Human
None
333803
Mouse IgG2B
MAB3360
100 µg
$240
Human SDNSF/MCFD2
Mouse
Human E-Selectin/CD62E Mouse Rat L-Selectin/CD62L
Rat
None
294301
Mouse IgG2B
MAB2357
100 µg
$240
None
Polyclonal
Goat IgG
AF2556
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2556
50 µg
$380
None
300812
Rat IgG2A
MAB2556
100 µg
$240
None
BBIG-E6
Mouse IgG1
BBA1
100 µg
$230
None
BBIG-E4
Mouse IgG1
BBA16
200 µg
$230
None
Polyclonal
Goat Serum
BBA18
500 µL
$195
None
BBIG-E4
Mouse IgG1
BBA2
None
Polyclonal
Chicken IgY
AF575
1 mg
$840
100 µg
$355
Biotin
Polyclonal
Chicken IgY
BAF575
50 µg
$380
None
Polyclonal
Goat IgG
AF977
100 µg
$355
None
Polyclonal
Goat IgG
AF1534
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1534
50 µg
$380
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
93
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE P-Selectin/CD62P
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Human
None
9.00E+01
Mouse IgG1
BBA30
200 µg
SIZE
PRICE $315
None
Polyclonal
Sheep IgG
AF1728
100 µg
$355
Semaphorin 3C
Mouse
Biotin
Polyclonal
Sheep IgG
BAF1728
50 µg
$380
Semaphorin 3E
Human
None
Polyclonal
Goat IgG
AF3239
100 µg
$355
Semaphorin 4D/CD100
Mouse
None
Polyclonal
Sheep IgG
AF5235
100 µg
$355
Human Semaphorin 6A
Mouse
Semaphorin 6B
Human
Semaphorin 6C
Mouse
Serpin A4/Kallistatin
Human
None
Polyclonal
Goat IgG
AF1146
100 µg
$355
None
Polyclonal
Goat IgG
AF1615
100 µg
$355
None
201932
Rat IgG2A
MAB1615
500 µg
$325
None
Polyclonal
Goat IgG
AF2094
100 µg
$355
None
Polyclonal
Goat IgG
AF2108
100 µg
$355
None
256631
Rat IgG2A
MAB2108
500 µg
$325
None
Polyclonal
Goat IgG
AF1669
100 µg
$355
Serpin A6
Mouse
None
Polyclonal
Goat IgG
AF4065
100 µg
$355
Serpin A8/Angiotensinogen
Human
None
Polyclonal
Goat IgG
AF3156
100 µg
$355
Serpin E1/PAI-1
Human
None
Polyclonal
Goat IgG
AF1786
100 µg
$355
Serpin F1/PEDF
Human
None
Polyclonal
Goat IgG
AF1177
100 µg
$355
None
187003
Mouse IgG2B
MAB1177
500 µg
$325
None
Polyclonal
Goat IgG
AF1239
100 µg
$355
Serpin F2/a2-Antiplasmin
Mouse
Biotin
Polyclonal
Goat IgG
BAF1239
50 µg
$380
Serum Amyloid A1
Mouse
None
Polyclonal
Goat IgG
AF2948
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF3856
100 µg
$355
Serum Amyloid A4
Serum Amyloid A4 in Human Liver. Serum amyloid A4 (SAA4) was detected in paraffin-embedded human liver tissue sections using anti-human serum amyloid A4 affinity-purified polyclonal antibody (Catalog # AF3856) and Antigen Retrieval Reagent - Basic (Catalog # CTS013). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
SF-1/NR5A1
Human
None
N1665
Mouse IgG1
PP-N1665-00
100 µg
$380
None
Polyclonal
Goat IgG
AF592
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF592
50 µg
$380
None
Polyclonal
Goat IgG
AF2656
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2656
50 µg
$380
257812
Rat IgG2A
MAB2317
100 µg
$240
sFRP-3
Mouse
SHBG
Human
SHIP aa 874-941
Human/Mouse/Rat
None
Human
None
255402
Rat IgG2A
MAB1878
100 µg
$240
Human/Mouse/Rat
None
427043
Mouse IgG2B
MAB18781
100 µg
$240
AF3790
100 µg
$285
SHP-1 aa 205-595
SHP-1 in Human Thymus. Src-Homology 2 domain Phosphatase-1 (SHP-1) was detected in a paraffinembedded human thymus section using antihuman/mouse/rat SHP-1 monoclonal antibody (Catalog # MAB18781). The tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
Phospho-SHP-2 (Y542) Siglec-2/CD22 Siglec-5
94
Human/Mouse
None
Polyclonal
Rabbit IgG
Human
None
219934
Mouse IgG1
MAB1968
100 µg
$240
Mouse
None
Polyclonal
Goat IgG
AF2296
100 µg
$355
Human
None
194117
Mouse IgG2B
MAB1072
500 µg
$325
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Siglec-5/14 Siglec-7
Siglec-9
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Human
None
Polyclonal
Goat IgG
AF1072
100 µg
$355
None
Polyclonal
Goat IgG
AF1138
100 µg
$355
None
194212
Mouse IgG2B
MAB1138
500 µg
$325
None
Polyclonal
Goat IgG
AF1139
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1139
50 µg
$380
Human
Human
SIZE
PRICE
SKI
Human
None
Polyclonal
Goat IgG
AF4025
100 µg
$355
SLAIN1 aa 262-483
Human/Mouse
None
Polyclonal
Goat IgG
AF5638
100 µg
$355
Polyclonal
Goat IgG
AF2798
100 µg
$355
SLAIN1 in Mouse D3 Cells. SLAIN1 was detected in the D3 mouse embryonic stem cell line using antihuman/mouse SLAIN1 affinity-purified polyclonal antibody (Catalog # AF5638). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
Sleeping Beauty Transposase
Multi-species
SLITRK1
Human
SLPI
Human
None None
324622
Mouse IgG1
MAB2798
100 µg
$240
None
327829
Mouse IgG2B
MAB30091
100 µg
$240
None
Polyclonal
Total Goat IgG
AB-260-NA
1 mg
$355
None
Polyclonal
Goat IgG
AF1274
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1274
50 µg
$380
SMAC/Diablo
Human
None
Polyclonal
Goat IgG
AF7891
100 µg
$355
Smad1 aa 2-454
Human
None
Polyclonal
Goat IgG
AF2039
100 µg
$355
Smad2 aa 20-108
Human
None
376520
Mouse IgG2B
MAB4037
100 µg
$240
Smad3 aa 2-230
Human
None
378611
Rat IgG1
MAB4038
100 µg
$240
$355
Smad3 in Human Pancreatic Cancer. Smad3 was detected in paraffin-embedded human pancreatic cancer sections using anti-human Smad3 monoclonal antibody (Catalog # MAB4038). Tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown). Tissue was counterstained with hematoxylin (blue).
Smad4 aa 139-332
Human
None
Polyclonal
Goat IgG
AF2097
100 µg
Smad4 aa 139-332
Human
None
253343
Mouse IgG2B
MAB2097
100 µg
$240
Smad5 aa 155-258
Human
None
Polyclonal
Goat IgG
AF2250
100 µg
$355
Smad5 in Human Colon. Smad5 was detected in paraffin-embedded human colon tissue sections using anti-human Smad5 affinity-purified polyclonal antibody (Catalog # AF2250). Tissues were stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
95
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE Smad7 aa 320-398
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human/Mouse/Rat
None
293039
Mouse IgG2B
MAB2029
100 µg
$240
Polyclonal
Goat IgG
AF2309
100 µg
$355
Smad7 in Human Liver Cancer. Smad7 was detected in paraffin-embedded human liver cancer sections using anti-human/mouse/rat Smad7 monoclonal antibody (Catalog # MAB2029). Tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
Smad8 aa 158-223 SMAGP
Human
None
Human
None
Polyclonal
Goat IgG
AF3959
100 µg
$355
Rat
None
Polyclonal
Goat IgG
AF3967
100 µg
$355
1A4
Mouse IgG2A
MAB1420
100 µg
$240
SMAGP in Human Colon. Small Transmembrane and Glycosylated Protein (SMAGP) was detected in paraffin-embedded human colon sections using antihuman SMAGP affinity-purified polyclonal antibody (Catalog # AF3959). Tissue was stained using the antigoat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
a-Smooth Muscle Actin N-terminal Synthetic Decapeptide
Human
None
Snail
Human
None
Polyclonal
Goat IgG
AF3639
100 µg
$355
SNF1LK2
Human
None
Polyclonal
Goat IgG
AF5737
100 µg
$355
SOCS-3 aa 1-225
Human/Mouse
None
516919
Mouse IgG2B
MAB5696
100 µg
$240
SOCS-5
Human
None
Polyclonal
Goat IgG
AF4796
100 µg
$355
SOD1/Cu-Zn SOD
Human
None
348808
Mouse IgG2A
MAB3418
100 µg
$240
SOD2/Mn-SOD aa 25-222
Human/Mouse/Rat
None
349810
Mouse IgG1
MAB3419
100 µg
$240
Somatostatin R2/SSTR2
Human
None
402038
Mouse IgG2A
MAB4224
100 µg
$240
Sonic Hedgehog C-Terminus
Mouse
Sonic Hedgehog N-Terminus
Mouse
None
Polyclonal
Goat IgG
AF445
100 µg
$355
None
Polyclonal
Goat IgG
AF464
100 µg
$355
None
171018
Rat IgG2A
MAB4641
500 µg
$325
SorCS1 aa 111-1099
Human
None
Polyclonal
Goat IgG
AF3457
100 µg
$355
SorCS2
Mouse
None
Polyclonal
Sheep IgG
AF4237
100 µg
$355
SorCS3
Mouse
None
Polyclonal
Goat IgG
AF3067
100 µg
$355
SorCS3 aa 34-1122
Mouse
None
339615
Rat IgG2A
MAB30671
100 µg
$240
SorLA
Human
None
525122
Mouse IgG1
MAB5699
100 µg
$240
Human
None
Polyclonal
Goat IgG
AF3154
100 µg
$355
Sortilin
Mouse
SOST/Sclerostin
Mouse
SOX1
Human
SOX2
Human Human/Mouse
96
None
Polyclonal
Goat IgG
AF2934
100 µg
$355
None
Polyclonal
Goat IgG
AF1589
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1589
50 µg
$380
None
Polyclonal
Goat IgG
AF3369
100 µg
$355
None
Polyclonal
Goat IgG
AF2018
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2018
50 µg
$380
None
245610
Mouse IgG2A
MAB2018
100 µg
$240
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES
SOX2 NorthernLights-conjugated
Human/Mouse
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
NL493
245610
Mouse IgG2A
NL2018G
500 µL
$345
NL557
245610
Mouse IgG2A
NL2018R
500 µL
$345
NL637
245610
Mouse IgG2A
NL2018V
500 µL
$345
None
Polyclonal
Goat IgG
AF2569
100 µg
$355
None
287403
Mouse IgG2A
MAB2569
100 µg
$240
SOX2 in Human BG01V Cells. SOX2 was detected in the human BG01V embryonic stem cell line using anti-human/mouse SOX2 NorthernLights-637-conjugated monoclonal antibody (Catalog # NL2018V; red). Cells were counterstained with DAPI (blue). The human BG01V embryonic stem cell line was grown on Irradiated Mouse Embryonic Fibroblasts (iMEF; Catalog # PSC001).
SOX3
Human
SOX7
Human
SOX9
Human
None
Polyclonal
Goat IgG
AF2766
100 µg
$355
None
Polyclonal
Goat IgG
AF3075
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF3075
50 µg
$380
SOX9 in Human HEK293 Cells. SOX9 was detected in human HEK293 embryonic kidney cell line using anti-human SOX9 affinity-purified polyclonal antibody (Catalog # AF3075). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
SOX9 NorthernLights-conjugated
Human
NL557
Polyclonal
Goat IgG
NL3075R
500 µL
$350
Human
None
Polyclonal
Goat IgG
AF2864
100 µg
$355
Human/Rat
None
20B7
Mouse IgG1
MAB2864
100 µg
$240
SOX10 NorthernLights-conjugated
Human/Rat
NL557
20B7
Mouse IgG1
NL2864R
500 µL
$345
SOX15
Human
None
Polyclonal
Sheep IgG
AF4070
100 µg
$355
None
Polyclonal
Goat IgG
AF1924
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1924
50 µg
$380
None
245013
Mouse IgG3
MAB1924
100 µg
$240
NL557
Polyclonal
Goat IgG
NL1924R
500 µL
$350
SOX10
SOX17
Human
SOX17 NorthernLights-conjugated, aa 177-144
Human
SOX17 in Mouse Melanoma Cells. SOX17 was detected in the B16 mouse melanoma cell line using anti-human SOX17 affinity-purified polyclonal antibody (Catalog # AF1924). The cells were stained with NorthernLights-557 anti goat secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
SOX21
SPARC/Osteonectin
Spinesin
Phospho-Src (Y419)
Human Human
None
Polyclonal
Goat IgG
AF3538
100 µg
$355
None
Polyclonal
Goat IgG
AF941
100 µg
$355
None
122511
Mouse IgG1
MAB941
500 µg
$325
Mouse
None
124413
Rat IgG2B
MAB942
500 µg
$325
Human
None
Polyclonal
Goat IgG
AF2495
100 µg
$355
None
Polyclonal
Goat IgG
AF1928
100 µg
$355
Mouse Human
Biotin
Polyclonal
Goat IgG
BAF1928
50 µg
$380
None
Polyclonal
Rabbit IgG
AF2685
100 µg
$285
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
97
R&D SYSTEMS IHC/ICC ANTIBODIES SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Src
MOLECULE
Human
None
327554
Mouse IgG2A
MAB3807
100 µg
SIZE
PRICE $240
Src aa 1-79
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF3389
100 µg
$355
Src in Human Colon. Src was detected in paraffinembedded normal human colon sections using antihuman/rat/mouse Src affinity-purified polyclon al antibody (Catalog # AF3389). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
SSEA-1
Human/Mouse
None
MC-480
Mouse IgM
MAB2155
100 µg
$240
SSEA-1 NorthernLights-conjugated
Human/Mouse
NL557
MC-480
Mouse IgM
NL2155R
500 µL
$345
SSEA-3
Human/Mouse
None
MC-631
Rat IgM
MAB1434
100 µg
$230
SSEA-4
Human/Mouse
Biotin
MC-813-70
Mouse IgG3
BAM1435
100 µg
$295
None
MC-813-70
Mouse IgG3
MAB1435
100 µg
$230
STAT1
Human
None
246523
Rat IgG2B
MAB1490
100 µg
$240
STAT2
Human
None
545103
Mouse IgG2B
MAB16661
100 µg
$240
Phospho-STAT3 (Y705)
Human
None
Polyclonal
Rabbit IgG
AF4607
100 µg
$285
Polyclonal
Sheep IgG
AF17991
100 µg
$355
Phospho-STAT3 in Human Daudi Cells. PhosphoSignal Transducer and Activator of Transcription 3 (STAT3) was detected in human INF-a-treated Daudi human cells (Y705) treated with IFN-a using antihuman phospho-STAT3 affinity-purified polyclonal antibody (Catalog # AF4607). Cells were stained using NorthernLights-557 donkey anti-rabbit IgG secondary antibody (Catalog # NL004; red) and counterstained with DAPI (blue).
None
STAT3 aa 1-175
Human
None
232209
Mouse IgG2B
MAB1799
100 µg
$240
STAT3 aa 688-770
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF1799
100 µg
$355
STAT3 NorthernLights-conjugated
Human/Mouse/Rat
NL557
232209
Mouse IgG2B
NL1799R
500 µL
$345
Human
None
Polyclonal
Rabbit IgG
AF4190
100 µg
$285
Phospho-STAT5a/b (Y699)
Phospho-STAT5 (Y699) in IFN-α-treated Daudi cells. Phospho-Signal Transducer and Activator of Transcription 5 (Y699) was detected in IFN-a treated Daudi BurKitt’s lymphoma human cells using antihuman/mouse phosphor-STAT5 (Y699) polyclonal antibody (Catalog # AF4190). The cells were stained using NorthernLights-557 anti-rabbit secondary antibody (Catalog # NL004; red) and counterstained with DAPI (blue).
STAT5a aa 774-794 STAT6 aa 342-640
Human
None
251610
Mouse IgG1
MAB21741
100 µg
$240
Human/Mouse
None
251619
Mouse IgG3
MAB2174
100 µg
$240
Human
None
253906
Mouse IgG2A
MAB2167
100 µg
$240
STAT6 in Human Peripheral Blood Mononuclear Cells (PBMCs). Signal Transducer and Activator of Transcription 6 (STAT6) was detected in PHA-stimulated human PBMCs using anti-human/mouse STAT6 monoclonal antibody (Catalog # MAB2167; red). Cells were counterstained (green).
98
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF2566
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2566
50 µg
$380 $240
Stella/Dppa3
Mouse
None
283910
Rat IgG2A
MAB2566
100 µg
STRA6
Human
None
496613
Mouse IgG1
MAB5904
100 µg
$240
STRO-1
Human
None
STRO-1
Mouse IgM
MAB1038
100 µg
$295
Substance P
Human/Mouse/Rat
None
266815
Mouse IgG1
MAB4375
100 µg
$240
SUMO3
Human
None
401513
Rat IgG2A
MAB2959
100 µg
$240
Survivin
Human
None
Polyclonal
Rabbit IgG
AF886
100 µg
$285
SUZ12
Human
None
448909
Mouse IgG1
MAB4184
100 µg
$240
Synapsin I Pan Specific
Human/Mouse/Rat/Bovine
None
Polyclonal
Rabbit IgG
PPS035
10 µg
$355
Synaptophysin
Human
None
Polyclonal
Goat IgG
AF5555
100 µg
$355
Synaptotagmin
Rat
None
ASV48
Mouse IgG2B
MAB4364
100 µg
$240
None
ASV30
Mouse IgG2A
MAB43641
100 µg
$240
Synaptotagmin I in Rat Spinal Cord. Synapto tagmin I was detected in a cryostat section of rat spinal cord using anti-rat Synaptotagmin I monoclonal antibody (Catalog # MAB43641; red). The tissue was counterstained green.
Syndecan-1/CD138
Human Human
Syndecan-1/CD138 Mouse
Syndecan-2
Syndecan-3
Human Human
Polyclonal
Goat IgG
AF2780
100 µg
$355
Polyclonal
Goat IgG
BAF2780
50 µg
$380
None
359103
Rat IgG1
MAB2780
100 µg
$240
None
Polyclonal
Goat IgG
AF3190
100 µg
$355
None
300506
Rat IgG1
MAB2966
100 µg
$240
None
Polyclonal
Goat IgG
AF2965
100 µg
$355
None
305507
Rat IgG2A
MAB29651
100 µg
$240
None
374412
Rat IgG2A
MAB35391
100 µg
$240
Mouse
None
Polyclonal
Goat IgG
AF2734
100 µg
$355
Syndecan-3 Isoform 1
Human
None
Polyclonal
Goat IgG
AF3539
100 µg
$355
Syndecan-4
Human
None
Polyclonal
Goat IgG
AF2918
100 µg
$355
Syntaxin-BP1
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF5675
100 µg
$355
Synuclein-a
Human
None
Polyclonal
Goat IgG
AF1338
100 µg
$355
Synuclein-b
Human
None
Polyclonal
Sheep IgG
AF5528
100 µg
$355
None
516505
Mouse IgG2B
MAB5528
100 µg
$240
Synuclein-g
Human
None
514304
Mouse IgG1
MAB5745
100 µg
$240
TAB1 aa 7-159
Human/Mouse
None
Polyclonal
Goat IgG
AF3578
100 µg
$355
TACE/ADAM17 Cytosolic Domain
Human
None
Polyclonal
Goat IgG
AF2129
100 µg
$355
TACE/ADAM17 Ectodomain
Human
None
Polyclonal
Chicken IgY
AF930
100 µg
$355
Biotin
Polyclonal
Chicken IgY
BAF930
50 µg
$380
None
Polyclonal
Goat IgG
AF174
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF174
50 µg
$380
None
165609
Mouse IgG1
MAB174
500 µg
$325
None
Polyclonal
Goat IgG
AF1041
100 µg
$355
Human TACI/TNFRSF13B Mouse
None Biotin
Biotin
Polyclonal
Goat IgG
BAF1041
50 µg
$380
Tau
Multi-species
None
Polyclonal
Goat IgG
AF3494
100 µg
$355
T-bet/TBX21
Human
None
525803
Mouse IgG1
MAB5385
100 µg
$240
TBX2
Human
None
393720
Rat IgG2A
MAB50401
100 µg
$240
TBX3 aa 321-483
Human
None
Polyclonal
Goat IgG
AF4509
100 µg
$355
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
99
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE TBX6
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human
None
Polyclonal
Goat IgG
AF4744
100 µg
$355
Polyclonal
Goat IgG
AF3605
100 µg
$355
TBX6 in Mouse Embryonic Mesoderm. T-box Protein 6 (TBX6) was detected in E9.5 mouse embryonic tailbud presomitic mesoderm sections using antihuman TBX6 affinity-purified polyclonal antibody (Catalog # AF4744). Cells were stained using NorthernLights-557 donkey anti-goat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
TC21/R-Ras2
Human/Rat
None
TCCR/WSX-1
Human
None
Polyclonal
Goat IgG
AF1479
100 µg
$355
TCF-2/HNF-1b
Human
None
Polyclonal
Goat IgG
AF3330
100 µg
$355
TCF-7/TCF-1
Human
None
Polyclonal
Goat IgG
AF5596
100 µg
$355
TCL1A
Human
None
Polyclonal
Goat IgG
AF4847
100 µg
$355
TEM8 Isoform 1
Human
None
Polyclonal
Goat IgG
AF3886
100 µg
$355
None
Polyclonal
Goat IgG
AF3358
100 µg
$355 $240
Tenascin C
Tenascin R
Human
None
391819
Rat IgG2A
MAB3358
100 µg
Human/Mouse
None
578
Rat IgG2A
MAB2138
100 µg
$240
Human
None
Polyclonal
Goat IgG
AF3865
100 µg
$355
Mouse/Rat
None
619
Mouse IgG1
MAB1624
100 µg
$240
Biotin
TER-119
Rat IgG2B
BAM1125
250 µg
$380
None
TER-119
Rat IgG2B
MAB1125
500 µg
$325
None
415909
Mouse IgG1
MAB4407
100 µg
$240
Tenascin R in Human Brainstem. Tenascin R was detected in paraffin-embedded human brainstem (medulla) tissue sections using anti-human Tenascin R affinity-purified polyclonal antibody (Catalog # AF3865). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
TER-119 TFF3
Mouse Human
TFF3 in Human Colon. Trefoil factor 3 (TFF3) was detected in a paraffin-embedded human colon section using anti-human TFF3 monoclonal antibody (Catalog # MAB4407). The tissue was stained using the anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown) and counterstained with hematoxylin (blue).
TFPI-2 TGF-a
Human Human
LAP TGF-b1
Human
None
243220
Mouse IgG2A
MAB2545
500 µg
$325
None
Polyclonal
Total Goat IgG
AB-239-NA
1 mg
$355
None
Polyclonal
Goat IgG
AF-239-NA
100 µg
$355
None
Polyclonal
Total Goat IgG
AB-246-NA
1 mg
$355
None
Polyclonal
Goat IgG
AF-246-NA
100 µg
$355
TGF-b1
Multi-species
None
9016
Mouse IgG1
MAB240
500 µg
$325
TGF-b1, 2, 3
Multi-species
None
1D11
Mouse IgG1
MAB1835
500 µg
$325
TGF-b2
Multi-species
None
8607
Mouse IgG2B
MAB612
500 µg
$325
TGF-b2/1.2
Multi-species
TGF-b3
100
Multi-species
None
Polyclonal
Goat IgG
AF-302-NA
100 µg
$355
None
Polyclonal
Goat IgG
AF-243-NA
100 µg
$355
None
20724
Mouse IgG1
MAB243
500 µg
$325
None
44922
Mouse IgG1
MAB643
500 µg
$325
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
TGF-b RI/ALK-5
MOLECULE
Human
None
Polyclonal
Goat IgG
AF3025
100 µg
SIZE
PRICE $355
TGF-b RII
Human
None
Polyclonal
Goat IgG
AF-241-NA
250 µg
$355 $355
Thioredoxin-1
Human
None
Polyclonal
Goat IgG
AF1970
100 µg
Thioredoxin Reductase 2/TRXR2 aa 268-393
Human/Mouse
None
575322
Mouse IgG1
MAB5815
100 µg
$240
Thimet Oligopeptidase/THOP1
Human
None
Polyclonal
Sheep IgG
AF3439
100 µg
$355
Human
None
501733
Mouse IgG1
MAB3947
100 µg
$240
None
Polyclonal
Goat IgG
AF3894
100 µg
$355
Thrombomodulin/THBD/CD141
Mouse
Thrombospondin-1
Human
Tie-1
Human
Phospho-Tie-2 (Y1100)
Human/Mouse
Tie-2
None
461714
Rat IgG2B
MAB3894
100 µg
$240
None
301221
Mouse IgG2B
MAB3074
500 µg
$325
None
Polyclonal
Goat IgG
AF619
100 µg
$355
None
88016
Mouse IgG1
MAB619
500 µg
$325
None
Polyclonal
Rabbit IgG
AF3909
100 µg
$285
Human
None
Polyclonal
Goat IgG
AF313
100 µg
$355
Zebrafish
None
Polyclonal
Goat IgG
AF928
100 µg
$355
Tie-2 in Developing Blood Vessels of Zebrafish. Tie-2 was detected in the blood vessels of an embryonic zebrafish using anti-zebrafish Tie-2 affinity-purified polyclonal antibody (Catalog # AF928). The tissue was stained using NorthernLights-557 donkey antigoat IgG secondary antibody (Catalog # NL001; red) and counterstained with DAPI (blue).
TIM-1/KIM-1/HAVCR
Human
None
219211
Mouse IgG2B
MAB1750
500 µg
$325
Rat
None
Polyclonal
Goat IgG
AF3689
100 µg
$355
63515
Mouse IgG2B
MAB970
500 µg
$325
TIM-1 in Rat Kidney. T cell Immunoglobulin and Mucin domain-containing (TIM-1), also known as Kidney Injury Molecule 1 (KIM-1), was detected in frozen sections of rat kidney using anti-rat TIM-1 affinity-purified polyclonal antibody (Catalog # AF3689). Tissue was stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
Human TIMP-1
TIMP-2
None
Mouse
None
Polyclonal
Goat IgG
AF980
100 µg
$355
Rat
None
Polyclonal
Goat IgG
AF580
100 µg
$355
None
Polyclonal
Goat IgG
AF971
100 µg
$355
None
89025
Mouse IgG1
MAB971
500 µg
$325
Human
TIMP-2 in Human Ovarian Cancer. Tissue Inhibitor of Metalloproteinase 2 (TIMP-2) was detected in a paraffin-embedded tissue section of human ovarian cancer using anti-human TIMP-2 affinity-purified polyclonal antibody (Catalog # AF971). Tissues were stained with the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown), and counterstained with hematoxylin (blue).
TIMP-3
Human
None
183551
Mouse IgG1
MAB973
500 µg
$325
TIMP-4
Human
None
153934
Mouse IgG1
MAB974
500 µg
$325
TLR4
Human
TMEFF1/Tomoregulin-1
Mouse
None
Polyclonal
Goat IgG
AF1478
100 µg
$355
None
Polyclonal
Goat IgG
AF1995
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1995
50 µg
$380
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
101
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES Bovine
Canine
Equine TNF-a Human
Mouse Porcine
TNF-b/TNFSF1
TNF RI/TNFRSF1A
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF2279
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF2279
50 µg
$380
None
Polyclonal
Goat IgG
AF1507
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1507
50 µg
$380
None
236812
Mouse IgG1
MAB1507
500 µg
$325
None
Polyclonal
Goat IgG
AF1814
100 µg
$355
None
Polyclonal
Goat IgG
AF-210-NA
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF210
50 µg
$380
None
28401
Mouse IgG1
MAB610
500 µg
$325
None
Polyclonal
Goat IgG
AF-410-NA
100 µg
$355
None
Polyclonal
Goat IgG
AF690
100 µg
$355
None
103302
Mouse IgG1
MAB6903
100 µg
$240 $355
Rat
None
Polyclonal
Goat IgG
AF-510-NA
100 µg
Human
None
5802
Mouse IgG1
MAB211
500 µg
$325
Mouse
Biotin
Polyclonal
Goat IgG
BAF749
50 µg
$380
Human
None
Polyclonal
Goat IgG
AF225
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF-425-PB
100 µg
$355
Polyclonal
Total Goat IgG
AB-226-PB
1 mg
$355
TNF RI in Human Peripheral Blood Lymphocytes. Tumor Necrosis Factor Receptor I (TNF RI) was detected in human peripheral blood lymphocytes using anti-human TNF RI affinity-purified polyclonal antibody (Catalog # AF225; red) and cells were counterstained green.
TNF RII/TNFRSF1B
TOR
None
Human
None
22221
Mouse IgG2A
MAB226
500 µg
$325
Mouse
None
Polyclonal
Goat IgG
AF-426-PB
100 µg
$355
Human/Mouse
None
303728
Rat IgG2A
MAB1537
100 µg
$240
TOR in HEK293 cells. Target of rapamycin (TOR) expression was detected in HEK293 human embryonic kidney cells using anti-human TOR monoclonal antibody (Catalog # MAB1537). Cells were stained using NorthernLights-557 goat anti-rat IgG secondary antibody (Catalog # NL013; red) and counterstained with DAPI (blue).
TRa/NR1A1
Human
None
H2804
Mouse IgG2A
PP-H2804-00
100 µg
$380
TR4/NR2C2
Human
None
H0107B
Mouse IgG2A
PP-H0107B-00
100 µg
$380
Biotin
TRA-1-85
Mouse IgG1
BAM3195
100 µg
$240
TRA-1-85
Human
TRADD
Human
Human TRAIL/TNFSF10 Mouse
None
TRA-1-85
Mouse IgG1
MAB3195
100 µg
$240
None
Polyclonal
Goat IgG
AF2658
100 µg
$355
None
Polyclonal
Goat IgG
AF375
100 µg
$355
None
75411
Mouse IgG1
MAB375
500 µg
$325
None
75402
Mouse IgG1
MAB687
500 µg
$325
None
Polyclonal
Goat IgG
AF1121
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1121
50 µg
$380
TRAIL R1/TNFRSF10A
Human
None
Polyclonal
Goat IgG
AF347
100 µg
$355
TRAIL R2/TNFRSF10B
Mouse
None
118929
Rat IgG2A
MAB1540
500 µg
$325
102
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE TRAIL R3/TNFRSF10C
SPECIES Human
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF630
100 µg
$355
None
90905
Mouse IgG1
MAB6301
500 µg
$325
$355
TRAIL Receptor 3 in Human Spleen. Tumor Necrosis Factor-related Apoptosis-inducing Ligand Receptor 3 (TRAIL R3) was detected in paraffin-embedded human spleen tissue sections using anti-human TRAIL Receptor 3 monoclonal antibody (Catalog # MAB630). Tissues were subjected to antigen retrieval using basic reagent (Catalog # CTS013) and stained using anti-mouse HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown). Tissues were counterstained with hematoxylin (blue).
TRAIL R4/TNFRSF10D
Human
None
Polyclonal
Goat IgG
AF633
100 µg
TRANCE/TNFSF11/RANK L
Human
None
70525
Mouse IgG2B
MAB626
500 µg
$325
TRANCE/TNFSF11/RANK L
Mouse
None
Polyclonal
Goat IgG
AF462
100 µg
$355
TfR (Transferrin R)
Human
TREM-1
Mouse
Human TREM-2 Mouse Human TrkA
Rat Human
TrkB
Mouse
Human TrkC Mouse
None
Polyclonal
Goat IgG
AF2474
100 µg
$355
None
Polyclonal
Goat IgG
AF1187
100 µg
$355
Biotin
174031
Rat IgG2A
BAM1187
100 µg
$380
None
174031
Rat IgG2A
MAB1187
500 µg
$325
None
Polyclonal
Goat IgG
AF1828
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1828
50 µg
$380
None
Polyclonal
Sheep IgG
AF1729
100 µg
$355
Biotin
Polyclonal
Sheep IgG
BAF1729
50 µg
$380
None
Polyclonal
Goat IgG
AF175
100 µg
$355
None
Polyclonal
Goat IgG
AF1056
100 µg
$355 $380
Biotin
Polyclonal
Goat IgG
BAF1056
50 µg
None
75133
Mouse IgG2B
MAB397
500 µg
$325
None
Polyclonal
Goat IgG
AF1494
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1494
50 µg
$380
None
225105
Rat IgG2B
MAB1494
500 µg
$325
None
Polyclonal
Goat IgG
AF373
100 µg
$355
None
75219
Mouse IgG2B
MAB373
500 µg
$325
None
Polyclonal
Goat IgG
AF1404
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1404
50 µg
$380
TrkC in Rat Spinal Cord. TrkC was detected in a cryostat tissue section of rat spinal cord using antihuman TrkC monoclonal antibody (Catalog # MAB373; red).
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103
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
SPECIES Human
TROP-2 Mouse
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF650
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF650
50 µg
$380
None
Polyclonal
Goat IgG
AF1122
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1122
50 µg
$380
None
200805
Mouse IgG2A
MAB1874
100 µg
$240
None
Polyclonal
Goat IgG
AF723
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF723
50 µg
$380
Troponin T (Cardiac)
Human
TROY/TNFRSF19
Mouse
Trypsin Pan Specific (PRSS1/2/3)
Human
None
Polyclonal
Sheep IgG
AF3586
100 µg
$355
Trypsin 1/PRSS1
Human
None
Polyclonal
Sheep IgG
AF3848
100 µg
$355
Trypsin 3/PRSS3
Mouse
None
Polyclonal
Goat IgG
AF3565
100 µg
$355
None
419911
Rat IgG1
MAB3565
100 µg
$240
Trypsin 3 in Mouse Pancreas. Trypsin 3 was detected in a frozen section of mouse pancreas using antimouse Trypsin-3 monoclonal antibody (Catalog # MAB3565). Tissues were stained using anti-rat HRPDAB reagents (brown) and counterstained with hematoxylin (blue).
Trypsin-3 in Mouse Kidney. Trypsin-3 was detected in frozen mouse kidney sections using anti-mouse Trypsin-3 monoclonal antibody (Catalog # MAB3565). Tissue was stained using the anti-rat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS017; brown) and counterstained with hematoxylin (blue).
Tryptophan Hydroxylase Pan Specific
Multi-species
None
Polyclonal
Sheep IgG
PPS038
100 µL
$355
Tryptophan Hydroxylase 1
Human
None
Polyclonal
Goat IgG
AF5276
100 µg
$355
TSG
Mouse
TSG
Mouse Human
TSG-6
Mouse
None
Polyclonal
Goat IgG
AF756
100 µg
$355
Biotin
120024
Rat IgG2A
BAM756
250 µg
$380
None
120024
Rat IgG2A
MAB756
500 µg
$325
None
Polyclonal
Goat IgG
AF2104
100 µg
$355
None
Polyclonal
Goat IgG
AF2326
100 µg
$355 $380
Biotin
Polyclonal
Goat IgG
BAF2326
50 µg
TSH b
Human
None
512913
Mouse IgG1
MAB5794
100 µg
$240
TSLP R
Human
None
Polyclonal
Goat IgG
AF981
100 µg
$355
TSPAN8
Human
None
458811
Rat IgG2B
MAB4734
100 µg
$240
Biotin
TuJ-1
Mouse IgG2A
BAM1195
100 µg
$380
None
TuJ-1
Mouse IgG2A
MAB1195
100 µg
$240
Neuron-specific b-III Tubulin
Multi-species
b-III Tubulin and Nestin in Rat Cortical Stem Cells. b-III Tubulin and Nestin were detected in rat cortical stem cells (Catalog # NSC001) using anti-neuron specific b-III Tubulin monoclonal (clone TuJ-1) antibody (Catalog # MAB1195; red) and anti rat Nestin affinitypurified polyclonal antibody (Catalog # AF2736; green). Cells were stained with NorthernLights-557 donkey anti-mouse IgG (Catalog # NL007) and NorthernLights-493 donkey anti-goat IgG (Catalog # NL003) secondary antibodies. Tissue was counterstained with DAPI (blue).
Neuron-specific b-III Tubulin NorthernLights-conjugated
Multi-species
TWEAK/TNFSF12
Human
TWEAK R/TNFRSF12
Phospho-Phospho-Tyrosine
104
b-III Tubulin and GFAP in Adult Mouse Brain. Neuronal b-III Tubulin and astrocytic GFAP were detected in adult mouse brain sections using anti-b-III Tubulin monoclonal antibody (Catalog # MAB1195; Tuj1) and anti-human GFAP affinity-purified polyclonal antibody (Catalog # AF2594). The tissue was stained using NorthernLights-493 donkey anti-mouse (Catalog # NL009; green) and NorthernLights-557 donkey anti-sheep IgG (Catalog # NL010; red) secondary antibodies, respectively. The nuclei were stained using DAPI (blue)
NL557
TuJ-1
Mouse IgG2A
NL1195R
500 µL
NL637
TuJ-1
Mouse IgG2A
NL1195V
500 µL
$345 $345
Biotin
Polyclonal
Goat IgG
BAF1090
50 µg
$380
Human
None
Polyclonal
Goat IgG
AF1199
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF1610
100 µg
$355
None
179003
Mouse IgG1
MAB1676
500 µg
$325
None
216954
Rat IgG2A
MAB16761
500 µg
$325
Multi-species
For research use only. Not for use in diagnostic procedures.
R&D SYSTEMS IHC/ICC ANTIBODIES SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
Phospho-Tyrosine Hydroxylase (S19)
MOLECULE
Rat
None
Polyclonal
Rabbit IgG
PPS087
100 µL
SIZE
PRICE $355
Phospho-Tyrosine Hydroxylase (S31)
Mouse/Rat/Primate
None
Polyclonal
Rabbit IgG
PPS088
100 µL
$355
Phospho-Tyrosine Hydroxylase (S40)
Rat
None
Polyclonal
Rabbit IgG
PPS041
100 µL
$355
None
TH-2
Mouse IgG1
MAB1423
100 µg
$240
None
6D7
Mouse IgG1
PPS040
100 µL
$355 $240
Tyrosine Hydroxylase
Multi-species
Ubiquitin/Ubiquitin+1
Human
None
83406
Mouse IgG2B
MAB701
100 µg
Ubiquitin+1
Human
None
82001
Mouse IgG2A
MAB703
100 µg
$240
ULBP-2
Human
None
Polyclonal
Goat IgG
AF1298
100 µg
$355
UNC5H1
Rat
None
Polyclonal
Goat IgG
AF1405
100 µg
$355
UNC5H2
Rat
None
Polyclonal
Goat IgG
AF1006
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1006
50 µg
$380
None
Polyclonal
Goat IgG
AF1005
100 µg
$355
UNC5H3
Human
None
200414
Mouse IgG2B
MAB1005
500 µg
$325
u-Plasminogen Activator (uPA)/Urokinase
Human
None
Polyclonal
Goat IgG
AF1310
100 µg
$355
uPAR
Mouse
None
Polyclonal
Goat IgG
AF534
100 µg
$355
Human
None
Polyclonal
Sheep IgG
AF5144
100 µg
$355
Uromodulin
Uromodulin in Human Kidney. Uromodulin was detected in paraffin-embedded human kidney sections using anti-human Uromodulin affinity-purified polyclonal antibody (Catalog # AF5144). Tissue was stained using anti-sheep HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS019; brown) and counterstained with hematoxylin (blue).
Uteroglobin/SCGB1A1
Human
None
394324
Rat IgG1
MAB4218
100 µg
$240
UTF1
Human
None
Polyclonal
Goat IgG
AF3958
100 µg
$355
Human/Mouse
None
Polyclonal
Goat IgG
AF4828
100 µg
$355
VAMP-1
VAMP-1 in Mouse Brain. Vesicle-associated membrane protein 1 (VAMP-1) was detected in frozen sections of mouse brain (spinal cord) using anti-human/ mouse VAMP-1 affinity-purified polyclonal antibody (Catalog # AF4828). The tissue was stained using antigoat IgG NorthernLights-557-conjugated secondary antibody (Catalog # NL001; red) and counterstained green.
Vang-like Protein 1/VANGL1
Human
None
497707
Mouse IgG2B
MAB5476
100 µg
$240
Vanilloid R1/TRPV1
Rat
None
Polyclonal
Goat IgG
AF3066
100 µg
$355
Vanilloid R1 in Rat Dorsal Root Ganglion. Vanilloid R1 was detected in a frozen section of rat dorsal root ganglion using anti-rat Vanilloid R1 affinity-purified polyclonal antibody (Catalog # AF3066; red) and counterstained green.
Vanilloid R-like 3/TRPV3
Human
None
Polyclonal
Sheep IgG
AF4167
100 µg
$355
VASA
Human
None
Polyclonal
Goat IgG
AF2030
100 µg
$355
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
105
R&D SYSTEMS IHC/ICC ANTIBODIES MOLECULE
VCAM-1/CD106
VDR/NR1I1 VEGF
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat Serum
BBA19
500 µL
$195
Human
None
BBIG-V1
Mouse IgG1
BBA5
100 µg
$230
None
BBIG-V1
Mouse IgG1
BBA6
1 mg
$840
Mouse
None
Polyclonal
Goat IgG
AF643
100 µg
$355
Human
None
H4537
Mouse IgG2A
PP-H4537-00
100 µg
$380 $325
Canine
None
247109
Mouse IgG1
MAB1603
500 µg
Human
None
26503
Mouse IgG2B
MAB293
500 µg
$325
None
Polyclonal
Goat IgG
AF1603
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF1603
50 µg
$380
Canine VEGF 164
Mouse
None
Polyclonal
Goat IgG
AF-493-NA
100 µg
$355
Rat
None
Polyclonal
Goat IgG
AF564
100 µg
$355
VEGF in Mouse Kidney. Vascular Endothelial Growth Factor (VEGF) was detected in a cryostat section of mouse kidney glomerulus using anti-mouse VEGF affinity-purified polyclonal antibody (Catalog # AF493-NA; red) and counterstained green.
VEGF in Rat Kidney. Vascular Endothelial Growth Factor (VEGF) was detected in a cryostat section of rat kidney glomerulus using anti-rat VEGF 164 affinitypurified polyclonal antibody (Catalog # AF564). Tissues were stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
None
Polyclonal
Total Goat IgG
AB-293-NA
1 mg
$355
None
Polyclonal
Goat IgG
AF-293-NA
100 µg
$355
Human
None
56-1
Mouse IgG1
MAB3045
500 µg
$325
Human/Mouse
None
58013
Mouse IgG1
MAB751
500 µg
$325
VEGF 165
Human
VEGF 165b VEGF-B 167/186 VEGF-B 186
Mouse
None
Polyclonal
Goat IgG
AF767
100 µg
$355
VEGF-C
Human
None
Polyclonal
Goat IgG
AF752
100 µg
$355
None
Polyclonal
Goat IgG
AF286
100 µg
$355
None
78923
Mouse IgG1
MAB286
500 µg
$325
None
Polyclonal
Goat IgG
AF469
100 µg
$355
None
Polyclonal
Goat IgG
AF321
100 µg
$355 $380
VEGF-D
Human Mouse
VEGF R1/Flt-1
Human Mouse Human
VEGF R2/KDR/Flk-1 Mouse
Biotin
Polyclonal
Goat IgG
BAF321
50 µg
None
141515
Rat IgG2A
MAB471
500 µg
$325
None
Polyclonal
Goat IgG
AF357
100 µg
$355
None
89115
Mouse IgG1
MAB3571
500 µg
$325
None
Polyclonal
Goat IgG
AF644
100 µg
$355
None
522302
Rat IgG1
MAB4432
100 µg
$240
None
Polyclonal
Goat IgG
AF349
100 µg
$355
None
54703
Mouse IgG1
MAB3491
500 µg
$325
VEGF R3/Flt-4
Human
Versican Isoform V0
Human
None
Polyclonal
Goat IgG
AF3054
100 µg
$355
VG5Q
Mouse
None
368011
Rat IgG2A
MAB30481
100 µg
$240
VHR
Human
None
237018
Mouse IgG1
MAB16541
100 µg
$240
None
Polyclonal
Goat IgG
AF2105
100 µg
$355
None
280618
Rat IgG2A
MAB2105
100 µg
$240
Vimentin
Human
Vimentin in Human Lung Carcinoma Cells. Vimentin was detected in cultured A549 human lung carcinoma cells cells using anti-human Vimentin monoclonal antibody (Catalog # MAB2105). The Cells were stained using NorthernLights-493 anti-rat secondary antibodies (Catalog # NL015; green) and the nuclei counterstained with DAPI (blue).
106
For research use only. Not for use in diagnostic procedures.
IHC/ICC SUPPLEMENTAL REAGENTS MOLECULE VIP R2 Vitronectin VLDL R VSIG1
SPECIES
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
Human
None
476031
Mouse IgG1
MAB5416
100 µg
$240
Human
None
342603
Mouse IgG2B
MAB2349
100 µg
$240
Mouse
None
347317
Rat IgG2A
MAB38751
100 µg
$240
Mouse
None
Polyclonal
Goat IgG
AF2258
100 µg
$355
Human
None
Polyclonal
Sheep IgG
AF4818
100 µg
$355
VSIG1 in Human Testis. V-set and Ig domain-containing protein 1 (VSIG1) was detected in frozen a section of human testis using anti-human VSIG1 affinity-purified polyclonal antibody (Catalog # AF4818). Cells were stained using NorthernLights-557 donkey anti-sheep IgG secondary antibody (Catalog # NL010; red) and counterstained with DAPI (blue).
WASP
WIF-1
Human
None
336925
Mouse IgG1
MAB3070
100 µg
$240
Human/Mouse
None
Polyclonal
Goat IgG
AF3070
100 µg
$355
None
133015
Mouse IgG1
MAB134-050
50 µg
$150
Human Human
WISP-1/CCN4
Wnt-1 Wnt-2
Mouse
None
133015
Mouse IgG1
MAB134-500
500 µg
$325
None
Polyclonal
Goat IgG
AF1627
100 µg
$355
None
Polyclonal
Sheep IgG
AF1680
100 µg
$355
Biotin
Polyclonal
Sheep IgG
BAF1680
50 µg
$380
Mouse
None
Polyclonal
Goat IgG
AF1620
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF3464
100 µg
$355
Wnt-2 in Human Stomach Cancer. Wnt-2 was detected in a paraffin-embedded human stomach cancer section using anti-human Wnt-2 affinity-purified polyclonal antibody (Catalog # AF3464). The section was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown). The tissue was counterstained with hematoxylin (blue).
Wnt-2b
Mouse
None
Polyclonal
Goat IgG
AF3900
100 µg
$355
Wnt-4
Mouse
None
Polyclonal
Goat IgG
AF475
100 µg
$355
Mouse
None
Polyclonal
Goat IgG
AF645
100 µg
$355
Wnt-5a
Wnt-5a in Embryonic Mouse Rib. Wnt-5a was detected in a paraffin-embedded tissue section of E15 mouse rib using anti-mouse Wnt-5a affinity-purified polyclonal antibody (Catalog # AF645). The tissue was stained using the anti-goat HRP-DAB (brown) Cell and Tissue Staining Kit (Catalog # CTS008) and counterstained with hematoxylin (blue).
Wnt-5b
Mouse
None
325004
Rat IgG2A
MAB3006
100 µg
$240
Wnt-6
Human
None
Polyclonal
Sheep IgG
AF4109
100 µg
$355
Wnt-7a
Human
None
Polyclonal
Goat IgG
AF3008
100 µg
$355
Wnt-7b
Human
None
Polyclonal
Goat IgG
AF3460
100 µg
$355
Wnt-8a
Mouse
None
Polyclonal
Goat IgG
AF2248
100 µg
$355
Wnt-8b
Human/Mouse
None
341504
Rat IgG2A
MAB3367
100 µg
$240
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
107
IHC/ICC SUPPLEMENTAL REAGENTS MOLECULE Wnt-9a
SPECIES Human
LABEL
CLONE
HOST/TYPE
CATALOG #
SIZE
PRICE
None
Polyclonal
Goat IgG
AF3157
100 µg
$355
None
326707
Mouse IgG2A
MAB31571
100 µg
$240
Wnt-9a in Human Breast Cancer. Wnt-9a was detected in a paraffin-embedded human breast cancer section using anti-human Wnt-9a monoclonal antibody (Catalog # MAB31571). The section was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS002; brown). The tissue was counterstained with hematoxylin (blue).
Wnt-9b
Mouse
None
Polyclonal
Goat IgG
AF3669
100 µg
$355
Wnt-11
Mouse
None
Polyclonal
Goat IgG
AF2647
100 µg
$355
XCL1/Lymphotactin
Mouse
None
Polyclonal
Goat IgG
AF486
100 µg
$355
Biotin
Polyclonal
Goat IgG
BAF486
50 µg
$380
XCR1
Human
None
Polyclonal
Goat IgG
AF857
100 µg
$355
Human
None
117318
Mouse IgG1
MAB8221
100 µg
$240 $355
XIAP
Yes aa 1-88 ZAP70 aa 163-254
Human/Mouse/Rat
None
Polyclonal
Goat IgG
AF8221
100 µg
Human/Mouse/Rat
None
339827
Mouse IgG2B
MAB32051
100 µg
$240
Human/Rat
None
Polyclonal
Goat IgG
AF3205
100 µg
$355
Human
None
Polyclonal
Goat IgG
AF3709
100 µg
$355
Polyclonal
Goat IgG
AF4978
100 µg
$355
ZAP70 in Human Lymphoma. Zeta-chain (TCR) associated protein kinase 70 (ZAP70) was detected in a paraffin-embedded human lymphoma section using anti-human ZAP70 affinity-purified polyclonal antibody (Catalog # AF3709). The tissue was subjected to antigen retrieval using the Basic Antigen Retrieval Kit (Catalog # CTS013) and stained using anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown). The section was counterstained with hematoxylin (blue).
ZIC1
Human/Mouse
None
ZIC1 in Embryonic Mouse Brain. Zinc-finger protein of the cerebellum 1 (ZIC1) was detected in a frozen section of E13 mouse brain using anti-human ZIC1 affinity-purified polyclonal antibody (Catalog # AF4978). The tissue was stained using the anti-goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
ZNF24
Human
None
Polyclonal
Goat IgG
AF3637
100 µg
$355
ZNF206
Human
None
Polyclonal
Goat IgG
AF4020
100 µg
$355
108
For research use only. Not for use in diagnostic procedures.
IHC/ICC SUPPLEMENTAL REAGENTS Secondary & Control Antibodies for IHC/ICC SECONDARY ANTIBODIES analyte Chicken IgY
Goat IgG
Goat IgG (H+L)
Hamster IgG
type
applications
catalog #
size
price
Chicken IgY specific polyclonal goat IgG
Western blot
AF010
500 µg
$75
Biotin
Chicken IgY specific polyclonal goat IgG
Western blot
BAF010
250 µg
$75
None
Goat IgG specific polyclonal donkey IgG
Western blot
AF109
500 µg
$75
Biotin
Goat IgG specific polyclonal donkey IgG
Western blot
BAF109
250 µg
$75
Biotin
Goat IgG specific polyclonal rabbit IgG
Western blot
BAF017
250 µg
$75
Biotin
Goat IgG specific polyclonal chicken IgY
Western blot
BAF019
250 µg
$75
HRP
Goat IgG specific polyclonal donkey IgG
Western blot
HAF109
1 mL
$95
HRP
Goat IgG specific polyclonal rabbit IgG
Western blot
HAF017
1 mL
$95
HRP
Goat IgG specific polyclonal chicken IgG
Western blot
HAF019
1 mL
$95
APC
Goat IgG specific polyclonal donkey IgG
Flow Cytometry
F0108
100 Tests
$220
Fluorescein
Goat IgG specific polyclonal donkey IgG
Flow Cytometry
F0109
100 Tests
$140
PE
Goat IgG specific polyclonal donkey IgG
Flow Cytometry
F0107
100 Tests
$175
None
Goat IgG specific polyclonal rabbit IgG
Western blot
R-401-C-ABS
PerCP
Goat IgG specific polyclonal donkey IgG (H+L) chains
Flow Cytometry
APC
Monoclonal mouse IgG2B, clone # MAH1.12
PE
Monoclonal mouse IgG2B, clone # MAH1.12
None Biotin
500 µg
$75
F0124
100 Tests
$190
Flow Cytometry
F0121
100 Tests
$220
Flow Cytometry
F0120
100 Tests
$175
Monoclonal mouse IgG2B, clone # MAH1.12
Western blot
MAB011
500 µg
$165
Monoclonal mouse IgG2B, clone # MAH1.12
Western blot
BAM011
250 µg
$180
Human IgG Fc
None
Human IgG Fc specific polyclonal goat IgG
Western blot
G-102-C
100 µg
$355
Human IgG1 Fc
None
Human IgG1 Fc specific monoclonal mouse IgG1
Western blot
MAB110
500 µg
$325
Human IgM
None
Human IgM specific polyclonal goat IgG
Western blot
G-105-C
100 µg
$355
Human IgE
None
Human IgE specific polyclonal goat IgG
Western blot
G-107-C
100 µg
$355
Human IgG (H+L)
None
Human IgG specific polyclonal goat IgG (H+L) chains
Western blot
G-101-C-ABS
500 µg
$75
PerCP
Mouse IgG specific polyclonal goat IgG F(ab')2 (H+L) Fragment
Flow Cytometry
F0114
100 Tests
$155
None
Mouse IgG specific polyclonal goat IgG
Western blot
AF007
500 µg
$99
Biotin
Mouse IgG specific polyclonal goat IgG
Western blot
BAF007
250 µg
$100
Biotin
Mouse IgG specific polyclonal donkey IgG
Western blot
BAF018
250 µg
$75
HRP
Mouse IgG specific polyclonal goat IgG
Western blot
HAF007
1 mL
$105
HRP
Mouse IgG specific polyclonal donkey IgG
Western blot
HAF018
1 mL
$95
Mouse IgG Fc
None
Mouse IgG Fc specific polyclonal goat IgG
Western blot
G-202-C
100 µg
$355
Mouse IgG
None
Mouse IgG specific polyclonal donkey IgG
Western blot
D-201-C-ABS2
500 µg
$75
APC
Mouse IgG specific polyclonal goat IgG F(ab’)2 (H+L) Fragment
Flow Cytometry
F0101B
100 Tests
$175
Fluorescein
Mouse IgG specific polyclonal goat IgG F(ab’)2 (H+L) Fragment
Flow Cytometry
F0103B
100 Tests
$105
PE
Mouse IgG specific polyclonal goat IgG F(ab’)2 (H+L) Fragment
Flow Cytometry
F0102B
100 Tests
$135
None
Rabbit IgG specific polyclonal goat IgG
Western blot
AF008
500 µg
$75
Biotin
Rabbit IgG specific polyclonal goat IgG
Western blot
BAF008
250 µg
$75
HRP
Rabbit IgG specific polyclonal goat IgG
Western blot
HAF008
1 mL
$95
Mouse IgG
Mouse IgG F(ab’)2 (H+L)
Rabbit IgG
Rat IgG
Rat IgG F(ab’)2 Rat IgG F(ab’)2 (H+L)
label None
APC
Rabbit IgG specific polyclonal goat IgG
Flow Cytometry
F0111
100 Tests
$220
Fluorescein
Rabbit IgG specific polyclonal goat IgG
Flow Cytometry
F0112
100 Tests
$140
PE
Rabbit IgG specific polyclonal goat IgG
Flow Cytometry
F0110
100 Tests
$175
None
Rabbit IgG specific polyclonal donkey IgG
Western blot
D-301-C-ABS2
HRP
Rat specific polyclonal goat IgG
Western blot
HAF005
None
Rat IgG specific polyclonal goat IgG
Western blot
PerCP
Rat IgG specific polyclonal goat IgG F(ab')2 (H+L) Fragment
Biotin
Rat IgG specific polyclonal goat IgG
APC
500 µg
$75
1 mL
$105
AF005
500 µg
$100
Flow Cytometry
F0115
100 Tests
$190
Western blot
BAF005
250 µg
$100
Rat IgG specific polyclonal goat IgGF(ab’)2 (H+L) Fragment
Flow Cytometry
F0113
100 Tests
$210
Fluorescein
Rat IgG specific polyclonal goat IgG F(ab’)2 (H+L) Fragment
Flow Cytometry
F0104B
100 Tests
$120
PE
Rat IgG specific polyclonal goat IgG F(ab’)2 (H+L) Fragment
Flow Cytometry
F0105B
100 Tests
$175
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
109
IHC/ICC SUPPLEMENTAL REAGENTS SECONDARY ANTIBODIES analyte
Sheep IgG
Mouse IgM
label
type
applications
catalog #
size
price
Biotin
Sheep IgG specific polyclonal donkey IgG
Western blot
BAF016
250 µg
$75
HRP
Sheep IgG specific polyclonal donkey IgG
Western blot
HAF016
1 mL
$95
None
Sheep IgG specific polyclonal donkey IgG
Western blot
D-501-C-ABS
500 µg
$75
APC
Mouse IgM specific polyclonal goat IgG
Flow Cytometry
F0117
100 Tests
$220
Fluorescein
Mouse IgM specific polyclonal goat IgG
Flow Cytometry
F0118
100 Tests
$140
PerCP
Mouse IgM specific polyclonal goat IgG
Flow Cytometry
F0119
100 Tests
$190
PE
Mouse IgM specific polyclonal goat IgG
Flow Cytometry
F0116
100 Tests
$175
NorthernLights™ A line of fluorochrome-conjugated secondary reagents for IHC/ICC NorthernLights secondary antibodies are bright and fade-resistant, recognizing mouse, goat, sheep, rat, and rabbit IgG and chicken IgY. Like all R&D Systems antibodies, NorthernLights antibody conjugates are specific and deliver a high signal-to-noise ratio. Also available are streptavidin conjugates for labeling biotinylated antibodies. description
conjugate
catalog #
NorthernLights-557 Donkey Anti-Goat IgG, Cross-adsorbed
NorthernLights-637
Donkey Anti-Rabbit IgG, Cross-adjusted
Donkey Anti-Mouse IgG, Cross-adsorbed
size
price
NL001
500 µL
$115
NL002
500 µL
$115
NorthernLights-493
NL003
500 µL
$115
NorthernLights-557
NL004
500 µL
$115
NorthernLights-637
NL005
500 µL
$115
NorthernLights-493
NL006
500 µL
$115
NorthernLights-557
NL007
500 µL
$115
NorthernLights-637
NL008
500 µL
$115
NorthernLights-493
NL009
500 µL
$115
NorthernLights-557
NL010
500 µL
$115
NorthernLights-637
NL011
500 µL
$115
NorthernLights-493
NL012
500 µL
$115
NorthernLights-557
NL013
500 µL
$115
NorthernLights-637
NL014
500 µL
$115
NorthernLights-493
NL015
500 µL
$115
NorthernLights-557
NL016
500 µL
$115
NorthernLights-637
NL017
500 µL
$115
NorthernLights-493
NL018
500 µL
$115
Streptavidin NL493
NorthernLights-493
NL997
500 µL
$115
Streptavidin NL637
NorthernLights-637
NL998
500 µL
$115
Streptavidin NL557
NorthernLights-557
NL999
500 µL
$115
Donkey Anti-Sheep IgG, Cross-adsorbed
Goat Anti-Rat IgG
Goat Anti-Chicken IgY
ANTIBODY CONTROLS control
Goat IgG
label
type
catalog #
size
price
Fluorescein
Normal goat IgG
IC108F
200 Tests
$165
PE
Normal goat IgG
IC108P
200 Tests
$165
PerCP
Normal goat IgG
IC108C
200 Tests
$165
None
Normal goat IgG
AB-108-C
1 mg
$75
Biotin
Normal goat IgG
BAF108
100 µg
$75
Human IgG1 Fc
None
NS0-expressed recombinant protein
110-HG-100
100 µg
$325
Human IgG
None
Normal human IgG
1-001-A
1 mg
$75
None
Normal sheep IgG
5-001-A
1 mg
$75
APC
Normal sheep IgG
IC016A
200 Tests
$165
Fluorescein
Normal sheep IgG
IC016F
200 Tests
$165
Sheep IgG
110
For research use only. Not for use in diagnostic procedures.
IHC PROTOCOL GUIDE ANTIBODY CONTROLS, continued control
Mouse IgG1
Mouse IgG2A
Mouse IgG2A Fc
Mouse IgG2B
Mouse IgG3
Rabbit IgG
Rat IgG
Rat IgG1
Rat IgG2A
Rat IgG2B
label
type
catalog #
size
price
PerCP
Normal mouse IgG1, clone # 11711
IC002C
200 Tests
$165
APC
Normal mouse IgG1, clone # 11711
IC002A
200 Tests
$165
None
Normal mouse IgG1, clone # 11711
MAB002
500 µg
$170
Biotin
Normal mouse IgG1, clone # 11711
IC002B
200 Tests
$165
Fluorescein
Normal mouse IgG1, clone # 11711
IC002F
200 Tests
$165
PE
Normal mouse IgG1, clone # 11711
IC002P
200 Tests
$165
None
Normal mouse IgG2B, clone # 20102
MAB003
500 µg
$170
None
Normal mouse IgG2B, clone # 133304
MAB0031
500 µg
$170
PerCP
Normal mouse IgG2B, clone # 20102
IC003C
200 Tests
$165
APC
Normal mouse IgG2B, clone # 20102
IC003A
200 Tests
$165
Biotin
Normal mouse IgG2B, clone # 20102
IC003B
200 Tests
$165
Fluorescein
Normal mouse IgG2B, clone # 20102
IC003F
200 Tests
$165
PE
Normal mouse IgG2B, clone # 20102
IC003P
200 Tests
$165
None
NS0-expressed recombinant protein
4460-MG-100
100 µg
$325 $170
None
Normal mouse IgG2B, clone # 20116
MAB004
500 µg
None
Normal mouse IgG2B, clone # 133303
MAB0041
500 µg
$170
None
Normal mouse IgG2BB, clone # 73009
MAB0042
500 µg
$170
APC
Normal mouse IgG2B, clone # 133303
IC0041A
200 Tests
$165
Fluorescein
Normal mouse IgG2B, clone # 133303
IC0041F
200 Tests
$165
PE
Normal mouse IgG2B, clone # 133303
IC0041P
200 Tests
$165
PerCP
Normal mouse IgG2B, clone # 133303
IC0041C
200 Tests
$165
None
Normal mouse IgG3, clone # 133316
MAB007
500 µg
$170
APC
Normal Mouse IgG3, clone # 133316
IC007A
200 Tests
$165
Fluorescein
Normal Mouse IgG3, clone # 133316
IC007F
200 Tests
$165
PE
Normal Mouse IgG3, clone # 133316
IC007P
200 Tests
$165
APC
Normal rabbit IgG
IC105A
200 Tests
$165
Fluorescein
Normal rabbit IgG
IC105F
200 Tests
$165
PE
Normal rabbit IgG
IC105P
200 Tests
$165
None
Normal rabbit IgG
AB-105-C
1 mg
$75
None
Normal rat IgG
6-001-A
1 mg
$75
None
Normal rat IgG1, clone # 43414
MAB005
500 µg
$170
APC
Normal rat IgG1, clone # 43414
IC005A
200 Tests
$165
Biotin
Normal rat IgG1, clone # 43414
IC005B
200 Tests
$165
Fluorescein
Normal rat IgG1, clone # 43414
IC005F
200 Tests
$160
PE
Normal rat IgG1, clone # 43414
IC005P
200 Tests
$165
None
Normal rat IgG2A, clone # 54447
MAB006
500 µg
$170
APC
Normal rat IgG2A, clone # 54447
IC006A
50 µg
$165
Biotin
Normal rat IgG2A, clone # 54447
IC006B
50 µg
$165
Fluorescein
Normal rat IgG2A, clone # 54447
IC006F
200 Tests
$165
PE
Normal rat IgG2A, clone # 54447
IC006P
200 Tests
$165
PerCP
Normal rat IgG, clone # 54447
IC006C
200 Tests
$165
PerCP
Normal rat IgG, clone # 141945
IC013C
200 Tests
$165
APC
Normal rat IgG2B, clone # 141945
IC013A
200 Tests
$165
Biotin
Normal rat IgG2B, clone # 141945
IC013B
200 Tests
$165
Fluorescein
Normal rat IgG2B, clone # 141945
IC013F
200 Tests
$165
PE
Normal rat IgG2B, clone # 141945
IC013P
200 Tests
$165
None
Normal rat IgG2B, clone # 141945
MAB0061
500 µg
$170
Mouse IgG3
PerCP
Normal mouse IgG, clone # 133316
IC007C
200 Tests
$165
Mouse IgM
PerCP
Normal mouse IgM
IC015C
200 Tests
$165
Chicken IgY
None
Normal chicken IgY
AB-101-C
1 mg
$75
US & Canada (800) 343-7475 | Europe +44 (0)1235 529449 | China +86 (21) 52380373 | www.RnDSystems.com
111
IHC PROTOCOL GUIDE Staining Kits & Reagents for Cells & Tissues Cell and Tissue Staining Kits are intended for localization of antigens in a broad range of histological and cytological specimens. These kits are based on the formation of the Avidin-Biotin Complex (ABC) with primary antibodies that react with the tissue antigens under study. The kits are made in ready-to-use format including prediluted Secondary Biotinylated Antibodies, and HSS-HRP that eliminates the need for extra steps in the procedure, minimizes hands-on time, maximizes convenience, and reduces the risk of erroneous calculations. Cell & Tissue Staining Kits species
label
components
catalog #
Size
Price
Anti-goat Anti-mouse Anti-rabbit Anti-rat Anti-sheep
HRP-DAB System
Secondary Biotinylated Antibody, Streptavidin-HRP Conjugate, DAB Chromogen, DAB Chromogen Buffer, Blocking Reagents
CTS008 CTS002 CTS005 CTS017 CTS019
50 Tests
$240
Anti-goat Anti-mouse Anti-rabbit Anti-rat Anti-sheep
HRP-AEC System
Secondary Biotinylated Antibody, Streptavidin-HRP Conjugate, AEC Chromogen, AEC Chromogen Buffer, Blocking Reagents
CTS009 CTS003 CTS006 CTS018 CTS020
50 Tests
$240
product
description
catalog #
Size
Price
p27/Kip1 IHC Detection Kit
Includes reagents for antigen retrieval, blocking reagent, detection reagents, and a specific monoclonal primary antibody for human p27 antigen
CTS2256
50 Tests
$425
Size
Price
p27 in Human Prostate Cancer. p27 was detected in paraffin-embedded human prostate cancer sections using the Human p27 Immunohistochemistry Detection Kit (Catalog # CTS2256). Tissue was stained using anti-mouse HRP-DAB (brown) and counterstained with hematoxylin (blue).
Cell & Tissue Staining Reagents product
description
catalog #
Basic Antigen Retrieval Reagent
Antigen retrieval system that utilizes a heat-induced recovery of cell and tissue antigens.
CTS013
50 mL
$60
Acidic Antigen Retrieval Reagent
Antigen retrieval system that utilizes a heat-induced recovery of cell and tissue antigens.
CTS014
50 mL
$60
Universal Antigen Retrieval Reagent
Antigen retrieval system that utilizes a heat-induced recovery of cell and tissue antigens.
CTS015
50 mL
$60
Antigen Retrieval Reagent Sampler
All three reagents (CTS013, CTS014, CTS015) in one convenient pack.
CTS016
50 mL
$120
Avidin-Fluorescein
For use as a secondary reagent in immunofluorescent assays in conjunction with biotinylated primary labeling reagents.
F0030
100 Tests
$65
Streptavidin-Allophycocyanin
For use as a secondary reagent in immunofluorescent assays in conjunction with biotinylated primary labeling reagents.
F0050
100 Tests
$85
Streptavidin-Phycoerythrin
For use as a secondary reagent in immunofluorescent assays in conjunction with biotinylated primary labeling reagents.
F0040
100 Tests
$75
Streptavidin-Alkaline Phosphatase
For use in ELISA or Western blot assays.
AR001
1 mL
$145
DAB Enhancer
DAB Enhancer is a concentrated 50X metal based solution used to intensify the DABChromogen (Catalog Numbers # CTS002, CTS005, CTS008, CTS017). In the presence of the DAB Enhancer, the DAB Chromogen produces a clear brown-black staining with minimal background.
CTS010
3 mL
$45
Mounting Medium
Permanent aqueous mounting medium
CTS011
15 mL
$35
Control
112
Antigen Retrieval
Antigen Retrieval Improves Detection of Neuroplastin 65. Neuroplastin 65 was detected in human cerebellar tissue using anti-human/mouse Neuroplastin 65 affinity-purified polyclonal antibody (Catalog # AF5360). Tissue was pretreated with Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the anti-Goat HRP-DAB Cell and Tissue Staining Kit (Catalog # CTS008; brown) and counterstained with hematoxylin (blue).
For research use only. Not for use in diagnostic procedures.
Ordering Information Customers IN Usa & CANADA Online ordering is available for customers in the U.S.A. and Canada. After finding your products, click the shopping cart symbol to create an account or to make an online purchase. Website: www.RnDSystems.com Phone: 1-800-343-7475 Fax:
1-612-656-4400
Mail:
R&D Systems, Inc. 614 McKinley Place NE Minneapolis, MN 55413
International Distributors Please visit www.RnDSystems.com for a full list of international distributors.
For all purchases, please include the following information with your order • Name & Contact Information • Name of Institution • Delivery Address • Invoice Address • Purchase Order Number* or Credit Card Number • Quantity and Product Size • Product Catalog Number and Description • Name of Principal Investigator (optional) • Telephone Number • E-mail Address (optional) For European Customers: VAT Exemption Certificate (UK) or E.C. equivalent * New customers wishing to use a P.O. number, please call customer service at 1-800-343-7475 to set up an account.
New IHC/ICC Screensavers!
To download R&D Systems IHC/ICC image screensavers, please visit our website at: www.RnDSystems.com/go/Screensavers
USA & Canada R&D Systems, Inc. 614 McKinley Place NE Minneapolis, MN 55413, USA TEL: (800) 343-7475 (612) 379-2956 FAX: (612) 656-4400 E-MAIL:
[email protected] www.RnDSystems.com
Europe R&D Systems Europe Ltd. 19 Barton Lane, Abingdon Science Park Abingdon OX14 3NB, UK TEL: +44 (0)1235 529449 FAX: +44 (0)1235 533420 E-MAIL:
[email protected] www. RnDSystems.com
China R&D Systems China Co., Ltd. 24A1 Hua Min Empire Plaza 726 West Yan An Road, Shanghai, PRC 200050 TEL: +86 (21) 52380373 FAX: +86 (21) 52371001 E-MAIL:
[email protected] www.RnDSystemsChina.com.cn
International Distributors Please visit www.RnDSystems.com/go/Distributors for a full list of international distributors. CA103_IHCprotocol_MAR