ImmobiIization of Candida cylindracea Lipase on Colloidal Liquid Aphrons (CLAs) and Development of a Continuous CLA-Membrane Reactor G.J. Lye, 0. P. Pavlou, M. Rosjidi, and D. C. Stuckey* Department of Chemical Engineering and Chemical Technology, Imperial College of Science, Technology and Medicine, Prince Consort Road, London, SW7 2BY, UK; e-mail:
[email protected] Received December 28, 1994/Accepted January 23, 1996
A novel technique is described for the immobilization of Candida cylindracea lipase in the soapy-shell of colloidal liquid aphrons (CLAs). CLAs consist of a micron-sized solvent droplet surrounded by a thin, aqueous, soapyfilm and are stabilized by a mixture of nonionic and ionic surfactants. Retention of lipase within the CLAs is primarily determined by electrostatic interactions between the surface charges on the protein and those of the anionic surfactant used (SDS) because leakage of the lipase from dispersed CLAs was reduced a t low continuous phase pH (
