Singh et al. 2013 Supplementary Information for
Immobilization of L-arabinitol dehydrogenase onto aldehyde-functionalized silicon oxide nanoparticles for Lxylulose production
Raushan Singh1, Manish Tiwari1,2, Ranjitha Singh1, Jung-Rim Haw2*, and JungKul Lee1,2*
1
Department of Chemical Engineering, 2Institute of SK-KU Biomaterials, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul 143-701, Korea
*Author for correspondence. E-mail:
[email protected], Tel: +82-2-450-3505; Fax: +82-2-458-3504 E-mail:
[email protected], Tel: +82-2-450-3499; Fax: +82-2-458-0879
Singh et al. 2013
Fig. S1. Purification of recombinant HjLAD. Enzyme fractions were analyzed by 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and visualized by staining with Coomassie blue R250. Lane M, Molecular mass Marker; Lane 1-9, elution fractions of purified recombinant HjLAD. Cell pellet obtained from 200 ml of cell culture was used for purification of HjLAD. A total of 5 µl purified enzyme was loaded from each elution fraction in the SDS-PAGE. The specific activity of HjLAD was 84.5 U/mg of protein.
Singh et al. 2013
Fig. S2. HPLC analysis of L-xylulose production from L-arabinitol by the immobilized HjLAD. The peaks corresponding to the L-arabinitol and L-xylulose are indicated.
Singh et al. 2013 Table S1. Number of lysine residues present on the surface of enzyme to facilitate the immobilization procedure. Number of lysine given in the table corresponds to the monomeric form of each enzyme.
Enzyme
Organism
Quaternary Structure
No. of surface lysine
Flavin-dependent Noxygenase
Actinomadura kijaniata
Monomer
3
Malate dehydrogenase
Haloarcula marismortui
Homotetramer
4
Versatile peroxidase
Pleurotus eryngii
Monomer
9
Thioredoxin reductase
Escherichia coli
Homodimer
11
Horseradish peroxidase
Armoracia rusticana
Monomer
4
Tryptophan 2,3dioxygenase
Xanthomonas campestris pv. campestris ATCC 33913
Homotetramer
3
Mannitol dehydrogenase
Pseudomonas fluorescens
Monomer
16
Sorbitol dehydrogenase
Rhodobacter sphaeroides
Homotetramer
4
Galactitol-1-phosphate dehydrogenase
Escherichia coli
Homodimer
14
Galactitol dehydrogenase
Rhodobacter sphaeroidesD
Homotetramer
1
Laccase
Coprinopsis cinerea
Monomer
5
Chloroperoxidase
Streptomyces aureofaciens
Homodimer
5
2,5-diketo-D-gluconic acid reductase A
Corynebacterium sp.
Monomer
7
Horseradish peroxidase C
Armoracia rusticana
Homohexamer
4
Erythromycin C-12 hydroxylase Estradiol 17 betahydroxysteroid dehydrogenase
Saccharopolyspora erythraea
Monomer
5
Homo sapiens
Homodimer
6
Bromoperoxidase A2
Streptomyces aureofaciens ATCC 10762
Homotrimer
4
Enoyl-ACP reductase
Mycobacterium tuberculosis
Homotetramer
8
Singh et al. 2013 Protoporphyrinogen oxidase
Myxococcus xanthus
Homodimer
6
Laccase
Melanocarpus albomyces
Monomer
7
Erythronate-4-phosphate dehydrogenase
Pseudomonas aeruginosa
Homodimer
2
Asparagine oxygenase
Streptomyces coelicolor
Monomer
1
Metal-beta-lactamase-1
Klebsiella pneumoniae
Monomer
6
Elastase
Sus scrofa
Monomer
3
Cellulase
Clostridium thermocellum
Monomer
9
Laminarinase
Phanerochaete chrysosporium
Monomer
3
Beta-mannanase
Bacillus agaradhaerens
Monomer
9
Haloalkane dehalogenase
Plesiocystis pacifica
Monomer
4
GH11 xylanase
Escherichia coli
Monomer
2
Cellulase cela
Alicyclobacillus acidocaldarius
Monomer
6
Endoglucanase V
Humicola insolens
Monomer
7
Cellobiohydrolase, CBH IB
Talaromyces emersonii
Monomer
11
Acetylcholinesterase
Homo sapiens
Heterodimer
7;3
Cutinase
Nectria haematococca mpVI
Monomer
6
Complement profactor D
Homo sapiens
Monomer
6
Uropepsin
Homo sapiens
Monomer
0
Beta-1,4-galactanase
Aspergillus aculeatus
Monomer
9
Glucose isomerase
Streptomyces sp. SK
Homotetramer
9
Phosphoheptose isomerase
Pseudomonas aeruginosa
Homotetramer
4
Mycobacterium tuberculosis
Homodimer
10
Ralstonia sp. U2
Homodimer
6
E. coli O157:H7
Homodimer
5
Triosephosphate isomerase Maleyl pyruvate isomerase Sugar isomerase
Singh et al. 2013
Phosphoribosyl isomerase
Mycobacterium tuberculosis
Monomer
3
Type B ribose 5phosphate isomerase Phenazine biosynthesis protein phzf Trna pseudouridine synthase
Trypanosoma cruzi
Homotetramer
5
Pseudomonas fluorescens 279
Homodimer
3
Thermus thermophilus HB8
Homodimer
9
D-xylose isomerase
Streptomyces rubiginosus
Homotetramer
9
Mycothiol-dependent maleylpyruvate isomerase Diaminopimelate epimerase
Corynebacterium glutamicum
Monomer
0
Escherichia coli K-12
Homodimer
7
Maleate isomerase
Nocardia farcinica IFM 10152
Homotetramer
1
Phosphoglycerate mutase
Mycobacterium tuberculosis
Homotetramer
4
Alanine racemase
Streptomyces lavendulae
Homodimer
3
Epoxide hydrolase
Streptomyces lasaliensis
Monomer
3
Leucyl-trna synthetase
Escherichia coli
Monomer
10
NAD+-dependent DNA ligase
Geobacillus stearothermophilus
Homodimer
10
Siah-1A protein
Mus musculus
Homodimer
6
Pantothenate synthetase
Mycobacterium tuberculosis
Homodimer
2
Chorismate synthase
Mycobacterium tuberculosis
Homotetramer
8
Polyketide synthase associated protein 5
Mycobacterium tuberculosis
Monomer
5
F-box only protein 2
Mus musculus
Monomer
6
Acyl-homoserine Lactone Synthase 4-Chlorobenzoate:coa ligase
Pantoea stewartii subsp. Stewartii
Monomer
6
Alcaligenes sp. AL3007
Homodimer
8
Magnesium-chelatase
Rhodobacter capsulatus
Homododecamer
3
Cysteinyl-trna synthetase
Mycobacterium smegmatis
Monomer
2
RNA 3'-terminal phosphate cyclase
Escherichia coli str. K-12 substr. MG1655
Monomer
6
Singh et al. 2013 Amino acid--[acyl-carrierprotein] ligase 1 Bira bifunctional protein
Bradyrhizobium japonicum USDA 110 Mycobacterium tuberculosis H37Rv
Homodimer
8
Monomer
0
Dethiobiotin synthetase
Escherichia coli
Homodimer
6
Glutamate-ammonialigase adenylyltransferase
Escherichia coli
Monomer
4
Phosphoribulokinase
Rhodobacter sphaeroides
Homooctamer
8
Escherichia coli
Homotetramer
7
Bos taurus
Homotrimer
8
Escherichia coli
Monomer
6
Paracoccus denitrificans
Homodimer
13
Mycobacterium tuberculosis H37Rv
Homodimer
5
uncultured organism
Homotrimer
5
Mycobacterium tuberculosis
Homotrimer
5
Arthrobacter ureafaciens
Monomer
3
Halobacterium salinarum
homohexamer
3
Cellulomonas sp.
Homotrimer
2
Herpes simplex virus (type 1 / strain 17)
Homodimer
2
Streptomyces purpurascens
Homodimer
3
Streptomyces cattleya
Homohexamer
6
Pseudomonas denitrificans
Homodimer
5
Mycobacterium tuberculosis
Homodimer
4
Cysteine synthase B
Mycobacterium tuberculosis
Homodimer
3
Nitrile hydratase
Rhodococcus sp. R312
Heterodimer
6;4
Methylglyoxal synthase
Escherichia coli
Homohexamer
3
Xanthine-guanine phosphoribosyltransferase Purine nucleoside phosphorylase Hydroxymethylbilane synthase Aromatic amino acid aminotransferase Phospho-2-dehydro-3deoxyheptonate aldolase Hexachlorocyclohexane dehydrochlorinase N-acetylglucosamine-1phosphate uridyltransferase Levan fructotransferase Nucleoside diphosphate kinase Purine nucleoside phosphorylase Thymidine kinase Aclacinomycin-10hydroxylase 5'-fluoro-5'deoxyadenosine synthase Uroporphyrinogen-III Cmethyltransferase Serine/threonine-protein kinase
Singh et al. 2013
Type II dehydroquinase
Streptomyces coelicolor
Homododecamer
1
Dialkylglycine decarboxylase Orotidine monophosphate decarboxylase Fuculose 1-phosphate aldolase (class II)
Burkholderia cepacia
Homotetramer
10
Methanothermobacter thermautotrophicus
Homodimer
6
Escherichia coli
Homotetramer
8
Benzaldehyde lyase
Pseudomonas fluorescens
Homotetramer
8
Carbonic anhydrase 9
Homo sapiens
Homodimer
1
Bordetella bronchiseptica
Monomer
2
Lyngbya majuscula
Homodimer
7
Epi-isozizaene synthase
Streptomyces coelicolor A3(2)
Monomer
5
Isochorismate synthase/Isochorismatepyruvate lyase mbti
Mycobacterium tuberculosis
Monomer
2
Sulfotransferase
Mycobacterium smegmatis
Homodimer
2
Homodimer
5
Homododecamer
1
Arylmalonate decarboxylase Curacin polyketide synthase
Orotidine 5'-phosphate decarboxylase 3-dehydroquinate dehydratase
Methanothermobacter thermautotrophicus str. Delta H Mycobacterium tuberculosis H37Rv
