Improved detection of natural hepatitis B virus ... - Wiley Online Library
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Improved detection of natural hepatitis B virus ... - Wiley Online Library
Jan 9, 2008 - Service of Diagnostic Microbiology, National Centre for Microbiology, Instituto de Salud Carlos III. Majadahonda,. Madrid, Spain. The sensitivity ...
Journal of Medical Virology 80:598–602 (2008)
Improved Detection of Natural Hepatitis B Virus Surface Antigen (HBsAg) Mutants by a New Version of the VITROS1 HBsAg Assay Jose´ M. Echevarrı´a* and Ana Avello´n Service of Diagnostic Microbiology, National Centre for Microbiology, Instituto de Salud Carlos III. Majadahonda, Madrid, Spain
The sensitivity of immunoassays for hepatitis B virus (HBV) surface antigen (HBsAg) detection may be hampered by the presence of mutants involving the major antigenic determinant of the protein. The performance of the VITROS1 HBsAg Assay has been shown to be affected by mutations comprising amino acid changes at residues 143, 144, and 145 of the HBsAg molecule. Sixtyseven serum samples from HBV carriers containing major populations of natural HBsAg mutants assayed previously by that assay were tested by the new VITROS1 HBsAg ES Assay. Samples displayed either single or multiple amino acid substitutions between positions 112 and 145 of the HBsAg, including changes in relevant residues such as 118–120, 125–127, and 143–145. Testing of undiluted samples by the current assay gave rise to false negative results in two samples displaying the single substitutions 145A and 145R, and in one additional sample displaying a dual mutation 118A þ 145A. Unusually weak reactivity (
