Communication In vitro digestibility of phenolics in grain of maize hybrids Kristina Kljak, Goran Kiš, Darko Grbeša Department of Animal Nutrition. University of Zagreb, Croatia Corresponding author: Dr. Kristina Kljak. Department of Animal Nutrition. University of Zagreb, Svetošimunska cesta 25, 10000 Zagreb, Croatia – Tel. +385 1 2393 945 – Fax: +385 1 2393 932 – Email:
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ABSTRACT - The aim of this research was to determine the quantity of phenolic compounds in the grain of maize hybrids and their in vitro release in pig small intestine. Six maize hybrids belonging to the FAO maturity groups 200-600 were produced under the same agroclimate conditions. Phenolics were determined using Folin-Ciocalteu colorimetric method as gallic acid equivalents. The enzymatic digestion of phenolics was carried out using in vitro procedure for pigs mimicking digestion in stomach and small intestine. The concentration (mg/100g) of free (100.02-157.29), bound (973.41-1160.86) and total phenolics (1105.821293.38) varied (P