African Journal of Biotechnology Vol. 11(48), pp. 10888-10893, 14 June, 2012 Available online at http://www.academicjournals.org/AJB DOI: 10.5897/AJB12.781 ISSN 1684–5315 ©2012 Academic Journals
Full Length Research Paper
In vitro propagation of Rosa hybrida L. cv. Al-Taif Rose plant Attia O. Attia1,2*, EL Dessoky S. Dessoky1,2 and Adel E. El-Tarras1 1
Biotechnology and Genetic Engineering Unit, Scientific Research Centre, Taif University, Taif, Kingdom of Saudi Arabia. 2 Plant Genetic Transformation Department, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Centre (ARC), 12619, Giza, Egypt. Accepted 18 May, 2012
In this study, a protocol for in vitro propagation of Rosa hybrida L. cv. Al-Taif Rose was established using nodal segments harboring axillary buds as explants. In vitro stages of shoot initiation, multiplication and elongation were performed. Explants were cultured on solid Murashige and Skoog medium (MS) supplemented with different concentrations of benzyl aminopurine (BAP, 1, 2 and 3 mg/l) in combination with 1 mg/l kinetin (Kn). Effect of different concentrations and combinations of indole-3acetic acid (IAA) and indole-3-butyric acid (IBA) on root formation of shoots were studied. The highest percentage of shoot initiation (85%) was observed on MS medium containing 2 mg/l BAP + 1 mg/l Kn, whereas maximum average number of multiplied shoots (2.7) was produced on MS medium with 3 mg/l BAP + 1 mg/l Kn. Highest average number of elongated shoots (26.7) was noticed on MS medium containing 1 mg/l BAP and 1 mg/l Kn. For rooting, highest percentage (66.7%) of rooted shoots was obtained on MS medium supplemented with 2 mg/l IBA. Plantlets with 4 to 5 roots of 3 to 5 cm length were successfully transferred to pots containing sterile peat moss for acclimatization. Key words: Rosa hybrid L. cv. Al-Taif Rose, axillary bud explants, in vitro propagation, multiplication, acclimatization. INTRODUCTION The genus Rosa is a member of the family Rosaceae which comprises more than 100 species (Horn, 1992). There are more than 20,000 commercial cultivars, which collectively are based on only eight wild species (Kim et al., 2003). Rosa hybrida L. cv. Al-Taif Rose is an important commercial cultivar among the scented roses in the Kingdom of Saudi Arabia, which yields a highly fragrant commercially valuable essential oil. The products of Al-Taif Rose, beside rose oil, are rose water and dried
*Corresponding author. E-mail:
[email protected]. Abbreviations: BAP, Benzyl aminopurine; IAA, indole-3-acetic acid; IBA, indole-3-butyric acid; Kn, kinetin; MS, Murashige and Skoog medium; NAA, α- naphthalene acetic acid; s.d.H2O, sterile distilled water.
petals that are used in medicine, perfume industry as well as make-up products (Nariman et al., 2011). Roses are generally multiplied vegetatively by grafting buds on stem of wild rose and by cuttings. This conventional method of propagation is very slow. Moreover, disease and environmental hazards make the cultivar degenerate gradually. So, this conventional process is not satisfactory in multiplication of Rosa spp (Gamborg and Phillips, 1995).Tissue culture methods have been developed as a potential tool for rapid and mass propagation in number of plant species (Khan and Shaw, 1988). Micropropagation offers not only quick propagation of plants, but also eliminates diseases and provides scope for development of new cultivars (Debergh and Read, 1990). The first report on rose shoot proliferation and rooting was made by Elliot (1970) and Jacob et al. (1970). There have been many reports on the proliferation of rose from shoot tips and meristems
Attia et al.
10889
Table 1. Effect of different concentrations and combinations of BAP and Kn on shoot initiation, multiplication and elongation of R. hybrida L. cv. Al-Taif Rose plant using axillary bud explants.
Growth regulator (mg/l) BAP Kn 0.0 0.0 1.0 1.0 2.0 1.0 3.0 1.0
NE
% IS
40 40 40 40
27.5 c 50.0 a 85.0 60.0b
d
ANS/E c
0.0 b 1.7 ab 2.0 2.7a
ANES b
00.0 a 26.7 a 21.7 18.3a
Means with the same letter are not significantly different (p
