Oct 10, 2015 - PhD3; 1Division of Infectious Diseases, David Geffen School of Medicine at UCLA, Los. Angeles, California; 2David Geffen School of Medicine ...
1737. In Vitro Susceptibility of Gemella Species From Clinical Isolates Jonathan Baghdadi, MD1; Theodoros Kelesidis, MD, PhD2; Romney Humphries, PhD3; 1Division of Infectious Diseases, David Geffen School of Medicine at UCLA, Los Angeles, California; 2David Geffen School of Medicine at UCLA, Los Angeles, California; 3Department of Pathology and Laboratory Medicine, UCLA, Los Angeles, California
(CTX), and meropenem (MEM). 13 (93%), 12 (86%) and 7 (50%) isolates were susceptible tovancomycin (VAN), clindamycin (CLI) and erythromycin (ERY)/levofloxacin (LVX), respectively. 7 of 8 (87.5%) patients with positive blood cultures were treated with β-lactams, and all cleared follow-up cultures. Zero patients with positive urine cultures received β-lactams, and only 1 (20%) was treated with a fluoroquinolone. The case of GS endophthalmitis was treated with intra-vitreous vancomycin.
Session: 242. Novel Diagnostics for Uncommon Bacteria Saturday, October 10, 2015: 12:30 PM
Table.
Background. Gemella species (GS) are normal human flora that can cause serious infections such as endocarditis. Minimum inhibitory concentration (MIC) breakpoints were recently established in the CLSI M45 A3 document. Published guidelines emphasize that antimicrobial susceptibility testing (AST) for GS is unpredictable. There is limited published evidence regarding interpretation of MIC breakpoints in GS isolates. Methods. 14 clinical isolates of GS from 2007 to 2015 were recovered from a single center. AST was performed by the CLSI broth microdilution method (BMD) in cationadjusted Muller Hinton broth supplemented with 2.5% lysed horse blood. BMD tests were incubated at 35C in 5% CO2 for 20 hours. MICs were interpreted according to M45 A3 breakpoints. Chart review was performed to document treatment and outcomes. Results. MIC data were available for 8 (57%) GS isolates from blood, 5 (36%) from urine, and 1 (7.1%) from vitreous fluid. All but a single isolate (from blood) were susceptible to all β-lactams, including penicillin (PEN), cefotaxime (CFX), ceftriaxone
No. Susceptible Isolates
Source (n)
PEN < 0.12
CFX
