INCREASED PRODUCTION OF NITRIC OXIDE STIMULATED BY ...
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INCREASED PRODUCTION OF NITRIC OXIDE STIMULATED BY ...
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British Journal of Rheumatology 1998;37:1123–1125
INCREASED PRODUCTION OF NITRIC OXIDE STIMULATED BY INTERLEUKIN-1b IN PERIPHERAL BLOOD MONONUCLEAR CELLS IN PATIENTS WITH SYSTEMIC SCLEROSIS T. YAMAMOTO,* Y. SAWADA, I. KATAYAMA and K. NISHIOKA Department of Dermatology, Tokyo Medical and Dental University School of Medicine, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113, Japan SUMMARY Objective. Nitric oxide (NO) is an important mediator of immune and inflammatory responses, and has recently been suggested to play some role in the pathogenesis of autoimmune disorders. In this study, we have examined whether peripheral blood mononuclear cells (PBMC ) from patients with systemic sclerosis (SSc) produce higher levels of NO spontaneously or in response to several stimulations in vitro. Methods. PBMC were obtained from 14 patients with SSc and 15 normal volunteers. Release of NO after stimulation with lipopolysaccharide (LPS), interleukin-1b (IL-1b), tumour necrosis factor alpha ( TNF-a) and interferon gamma (IFN-c) was determined by Griess reagents. Results. PBMC from SSc patients exhibited a higher level of spontaneous release of NO (13.4 ± 3.8 m) than those from control subjects (8.9 ± 1.6 m), but without significance. Incubation of PBMC for 24 h with stimulants caused an increase in NO production both in normal subjects and SSc patients. Stimulation with 10 U/ml IL-1b induced a significantly increased NO production in SSc patients (22.1 ± 6.6 m) compared with normal subjects (12.3 ± 4.0 m) (P < 0.05); however, in contrast, incubation with other stimulants showed no significant differences in NO production between SSc patients and normal subjects. Conclusion. These results suggest the abnormal regulation of NO production in PBMC of scleroderma patients in response to IL-1b, which might contribute, in part, to the fibrotic process in SSc. K : Nitric oxide, Systemic sclerosis, Cytokine.
The mean disease duration was ~5.6 yr. The clinical type of SSc, which was followed by the classification of Barnett et al. [6 ], was two patients with Type I, nine with Type II and three with Type III. Antitopoisomerase I antibody was detected in three patients. Three of the patients had been treated with systemic steroids (
