topoisomerase II activity and other topoisomerase II inhibitors, such as etoposide and teniposide, have been found to be effective for the treatment of gliomas (2, ...
ANTICANCER RESEARCH 25: 4203-4208 (2005)
Induction of Apoptosis and Cell Cycle Arrest in Glioma Cells by GL331 (a Topoisomerase II Inhibitor) YUN CHEN1, YEN-HAO SU2, CHIH-HSIEN WANG2, JIANN-MING WU1, JIN-CHERNG CHEN3 and SHENG-HONG TSENG2 1Department
of Surgery, Far Eastern Memorial Hospital, 21, Sec. 2, Nan-Ya South Road, Pan-Chiao, Taipei 220; of Surgery, National Taiwan University Hospital and National Taiwan University College of Medicine, 7, Chung-Shan S. Road, Taipei 100; 3Department of Surgery, Buddhist Tzu Chi Dalin General Hospital, 2, Ming Shen Road, Dalin, Chia-Yi, Taiwan
2Department
Abstract. Background: GL331 is a topoisomerase II inhibitor and, in this study, the effects of GL331 upon rat C6 glioma cells were investigated. Materials and Methods: The glioma cells were treated with GL331, then a cytotoxicity assay was done to evaluate the cytotoxic effects of GL331 and flow-activated cell sorter analysis was performed to analyze the cellular apoptosis and cell cycle distribution of the glioma cells. The expressions of p53, p21 and 14-3-3Û in glioma cells treated with GL331 were investigated by Western blot analysis. Results: GL331 was demonstrated to exert cytotoxic effects upon and induce apoptosis of the C6 glioma cells in a concentration- and timedependent manner (p
