Induction of Cytotoxicity and Apoptosis and ... - Semantic Scholar
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Induction of Cytotoxicity and Apoptosis and ... - Semantic Scholar
surface of apoptotic cells), showed a dose-dependent induction of early apoptosis by curcumin, which reached about 65% in. HL-60 cells and about 20% in HSG ...
ANTICANCER RESEARCH 25: 4029-4036 (2005)
Induction of Cytotoxicity and Apoptosis and Inhibition of Cyclooxygenase-2 Gene Expression, by Curcumin and its Analog, ·-Diisoeugenol TOSHIKO ATSUMI1, YUKIO MURAKAMI2, KAZUTOSHI SHIBUYA2, KEIICHI TONOSAKI1 and SEIICHIRO FUJISAWA2 1Department
2Diagnostic
of Human Development and Fostering and and Therapeutic Sciences, Meikai University School of Dentistry, Sakado, Saitama 350-0283, Japan
Abstract. Cytotoxicity, induction of apoptosis, ROS generation and inhibition of cyclooxygenase-2 (COX-2) gene expression by curcumin [1] and ·-diisoeugenol [2] were investigated. The cytotoxicity of curcumin and ·-diisoeugenol against human promyelocytic leukemia cells (HL-60 cells) and human submandibular cancer cells (HSG cells) was similar (CC50 1-3 ÌM). However, curcumin induced much more apoptosis, particularly in HL-60 cells compared with HSG cells, as revealed by measurement of the sub-G1/G0 DNA fraction in flow cytometric histograms. Treatment with 15 ÌM curcumin increased the number of cells with a sub-G1/G0 DNA fraction from control levels of
