Supplementary Material for: Induction of Invertebrate Larval Settlement; Different Bacteria, Different Mechanisms? Marnie L Freckelton, Brian T Nedved, and Michael G Hadfield* Kewalo Marine Laboratory, University of Hawaii, Honolulu, 96813, USA *
[email protected]
Experimental Scheme
Figure 1. Flow chart of methodology for both tailocin and extracellular vesicle assessments identifying bioassay samples. Methodology was followed in side by side preparations for all four bacterial species (P. luteoviolacea, C. lytica, B. aquimaris and S. warneri.
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Genomic Assessment Tables ORF Name MacS MacT2 MacT1 MacB (ORF1) ORF2 ORF3 JF50 12590 JF50 12595 JF50 12600 JF50 12605 JF50 12610 JF50 12615
Amino Acid Accession Num. AHX39703.1 AHX39702.1 AHX39701.1 AHX39695.1 AHX39694.1 AHX39693.1 AHX39721.1 AHX39720.1 AHX39719.1 AHX39718.1 AHX39717.1 AHX39716.1
Protein Name Phage Tail Sheath Protein FI t4-like Virus Tail Tube Protein t4-like Virus Tail Tube Protein Baseplate J-like Protein Hypothetical Protein Hypothetical Protein Hypothetical Protein Hypothetical Protein Hypothetical Protein Hypothetical Protein Hypothetical Protein Hypothetical Protein
Length (Amino Acids) 581 148 153 1070 890 1671 139 135 131 747 496 943
Table 1. Inductive genes from Pseudoalteromonas luteoviolacea. ORF, Open Reading Frame
ORF Name MacS MacT2 MacT1 MacB (ORF1) ORF2 ORF3 JF50 12590 JF50 12595 JF50 12600 JF50 12605 JF50 12610 JF50 12615
Query Coverage (%) 12 39 37 9 7 6 NA NA NA NA NA NA
Identity (%) 30 23 28 26 28 35 NA NA NA NA NA NA
e-Value 0.68 4.5 0.64 0.49 0.17 0.35 NA NA NA NA NA NA
Acession Number of Best Amino Acid Hit WP 051983455.1 WP 013621992.1 WP 013619596.1 WP 051983451.1 WP 051983452.1 WP 051983429.1 No Hits No Hits No Hits No Hits No Hits No Hits
Table 2. Summary of BLAST searches in genome of C. lytica. ORF, Open Reading Frame
ORF Name MacS MacT2 MacT1 MacB (ORF1) ORF2 ORF3 JF50 12590 JF50 12595 JF50 12600 JF50 12605 JF50 12610 JF50 12615
Query Coverage (%) 5 NA 13 4 NA NA 58 NA NA 8 6 5
Identity (%) 35 NA 43 23 NA NA 24 NA NA 33 46 37
e-Value 0.53 NA 2.3 3.7 NA NA 4.9 NA NA 2 4.5 4
Acession Number of Best Amino Acid Hit WP 044339398.1 No Hits WP 044339905.1 WP 044337912.1 No Hits No Hits WP 064090927.1 No Hits No Hits WP 032088594.1 WP 64091692.1 WP 64094184.1
Table 3. Summary of BLAST searches in genome of B. aquimaris. ORF, Open Reading Frame
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ORF Name MacS MacT2 MacT1 MacB (ORF1) ORF2 ORF3 JF50 12590 JF50 12595 JF50 12600 JF50 12605 JF50 12610 JF50 12615
Query Coverage (%) NA NA NA 2 NA 3 15 NA NA 2 NA 6
Identity (%) NA NA NA 3 NA 36 43 NA NA 45 NA 28
e-Value NA NA NA 0.098 NA 0.48 6.2 NA NA 2.1 NA 8.7
Acession Number of Best Amino Acid Hit No Hits No Hits No Hits WP 002451828.1 No Hits WP 002451828.1 WP 015364611.1 No Hits No Hits WP 002466879.1 No Hits WP 002466879.1
Table 4. Summary of BLAST searches in genome of S. warneri. ORF, Open Reading Frame
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