Influence of IGF-I on adhesion, proliferation, and galectin ... - CiteSeerX
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Influence of IGF-I on adhesion, proliferation, and galectin ... - CiteSeerX
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ORIGINAL ARTICLE Arch Oncol 2005;13(1):7-10.
UDC: 616-006.4:576.385.5
Influence of IGF-I on adhesion, proliferation, and galectin-1 production in JAr and Jeg-3 choriocarcinoma cell lines
®anka T. Bojiæ-Trbojeviæ, Miroslava M. Jankoviæ, Ljiljana M. Viæovac ABSTRACT BACKGROUND: JAr and Jeg-3 choriocarcinoma cell lines are model systems for the transformed trophoblast and allow studies of phenotype and regulatory factors for particular cell functions. Both cell lines express the receptor for insulin-like growth factor-I (IGF-I). Effects of IGF-I on adhesion, proliferation and galectin-1 production in JAr and Jeg-3 cells were studied. METHODS: The effects of IGF-I on proliferation and galectin-1 production were examined by thiazolyl blue assay and cell based solid phase assay using polyclonal anti-galectin-1 antibodies. The cell adhesion assay was performed on Matrigel coated wells. Galectin-1 production and localization was examined by immunocytochemistry. RESULTS: IGF-I decreased adhesion of JAr cells to 70% of the control value (p