yes a) No extinction. 7 na b) 2 CS-, 15 min ITI. 7 na c) 2 CS+, 15 min ITI. 7 na a) R58E02-GAL4 control CS- exposed. 8 yes b) R58E02-GAL4 control CS+ ...
Cell, Volume 175
Supplemental Information
Integration of Parallel Opposing Memories Underlies Memory Extinction Johannes Felsenberg, Pedro F. Jacob, Thomas Walker, Oliver Barnstedt, Amelia J. Edmondson-Stait, Markus W. Pleijzier, Nils Otto, Philipp Schlegel, Nadiya Sharifi, Emmanuel Perisse, Carlas S. Smith, J. Scott Lauritzen, Marta Costa, Gregory S.X.E. Jefferis, Davi D. Bock, and Scott Waddell
Table 1. Statistical details related to Figure 1-6 and S1-S7 Experiment / Figure
n
normally distributed
Figure 1A
a) No extinction b) 2 CS+, 1 min ITI c) 2 CS+, 15 min ITI d) 5 CS+, 1 min ITI
group/treatment
6 10 10 10
na yes yes yes
statistical test
ANOVA = 0.0025, Turkey's: a vs b = ANOVA with Tukey's multiple comparisons 0.99, a vs c = 0.02, a vs d = 0.04, b vs c test = 0.01, b vs d = 0.03, c vs d = 0.98
p value
Figure 1B
a) No extinction b) 2 CS-, 15 min ITI c) 2 CS+, 15 min ITI
7 7 7
na na na
ANOVA with Tukey's multiple comparisons test
Figure 1C
a) R58E02-GAL4 control CS- exposed b) R58E02-GAL4 control CS+ exposed c) UAS-shits control CS- exposed d) UAS-shits control CS+ exposed e) R58E02-GAL4;; UAS-Shits1 CS- exposed f) R58E02-GAL4;; UAS-Shits1 CS+ exposed
8 7 8 7 8 7
yes na yes na yes na
ANOVA (CS-) = 0.0081, Turkey's: a vs unpaired t-test for comparision between c = 0.0292, a vs e = 0.0104, c vs e = same genotype but different treatment, 0.8768, ANOVA (CS+) = 0.0046, ANOVA with Tukey's multiple comparisons Turkey's: b vs d = 0.88, b vs f = 0.02, d test for comparision between different vs f = 0.006, unpaired t-test: a vs b < genotype, same treatment 0.0001, c vs d = 0.02, e vs f = 0.4298
Figure 1 D
a) MB504B-GAL4 control CS- exposed b) MB504B-GAL4 control CS+ exposed c) UAS-Shits control CS- exposed d) UAS-Shits control CS+ exposed e) MB504B-GAL4;; UAS-Shits1 CS- exposed f) MB504B-GAL4;; UAS-Shits1 CS+ exposed
6 6 6 6 6 6
na na na na na na
unpaired t-test for comparision between same genotype but different treatment, ANOVA (CS-) = 0.8581, ANOVA (CS+) ANOVA with Tukey's multiple comparisons = 0.9763, unpaired t-test: a vs b = test for comparision between different 0.001, c vs d = 0.01, e vs f = 0.03 genotype, same treatment
g 5 g 5 control g4 b'2p b'2m
5 4 5 4 4
na na na na na
a) VT1211-GAL4 control CS+ exposed b) UAS-Shits1 control CS+ exposed c) VT1211-GAL4;; UAS-Shits1 CS+ exposed a) VT1211-GAL4 control CS- exposed c) UAS-Shits1 control CS- exposed e) VT1211-GAL4;; UAS-Shits1 CS- exposed
16 16 16 9 9 9
yes no yes yes yes yes
Figure 3A Figure 3B Figure 3C Figure 3D Figure 3E Figure 3F
MB112C-GAL4 dendrites training no extinction MCH as CS+ R39A05-GAL4 dendrites training no extinction MCH as CS+ R66C08-GAL4 dendrites training no extinction MCH as CS+ MB112C-GAL4 dendrites training extinction MCH as CS+ R39A05-GAL4 dendrites training extinction MCH as CS+ R66C08-GAL4 dendrites training extinction MCH as CS+
11 12 14 15 10 12
Figure 6A Figure 6B Figure 6C Figure 6D Figure 6E Figure 6F
VT1211-GAL4 axons no extinction MCH as CS+ R39A05-GAL4 axons training no extinction MCH as CS+ R66C08-GAL4 axons training no extinction MCH as CS+ VT1211-GAL4 axons training extinction MCH as CS+ R39A05-GAL4 axons training extinction MCH as CS+ R66C08-GAL4 axons training extinction MCH as CS+
Figure S1A
ANOVA = 0.0025, Turkey's: a vs b = 0.97, a vs c = 0.003, b vs c = 0.004
paired t-test paired t-test paired t-test paired t-test paired t-test
p = 0.0086 p = 0.2664 p = 0.2059 p = 0.061 p = 0.1805
ANOVA with Tukey's multiple comparisons test
ANOVA = 0.0108, Turkey's: a vs b = 0.99, a vs e = 0.02, b vs c = 0.02
ANOVA with Tukey's multiple comparisons test
ANOVA < 0.0001, Turkey's: a vs b = 0.88, a vs e < 0.0001, b vs c = 0.0003
no no no no yes yes
Wilcoxon matched-pairs signed rank test Wilcoxon matched-pairs signed rank test Wilcoxon matched-pairs signed rank test Wilcoxon matched-pairs signed rank test paired t-test paired t-test
p=0.0186 p=0.8501 p=0.6698 p=0.0125 p=0.3506 p=0.0019
15 14 18 14 16 11
no yes yes no no yes
Wilcoxon matched-pairs signed rank test paired t-test paired t-test Wilcoxon matched-pairs signed rank test Wilcoxon matched-pairs signed rank test paired t-test
p=0.0151 p=0.0378 p=0.0299 p=0.2166 p=0.0250 p=0.2594
a) 2 pre-exposures 1 min ITI, 10-3 b) 2 pre-exposures 15 min ITI, 10-3 c) 15 pre-exposures 1 min ITI, 10-3 d) 2 pre-exposures 15 min ITI, 10-4 a) 2 pre-exposures 1 min ITI, 10-6 b) 2 pre-exposures 15 min ITI, 10-6 c) 5 pre-exposures 1 min ITI, 10-6
7 8 8 8 11 11 11
na yes yes yes yes yes yes
One sample t test (theoretical mean = 0), ANOVA with Tukey's multiple comparisons test
t-test: p < 0.0001, ANOVA < 0.0001, Turkey's: a vs b < 0.0001, a vs c < 0.0001, b vs c = 0.246
10-3 10-6
6 6
na na
a) training/extinction/retraining/immediate test b) training/---/retraining/immediate test c) ---/---/retraining/immediate test
9 8 9
yes yes yes
a) training/extinction/retraining/ 90 min test b) training/---/retraining/ 90 min test c) ---/---/retraining/ 90 min test
9 9 9
yes yes yes
ANOVA with Tukey's multiple comparisons test
ANOVA = 0.0137, Turkey's: a vs b = 0.975, a vs c = 0.02, b vs c = 0.03
Figure S1D
a) R58E02-GAL4 control CS- exposed b) R58E02-GAL4 control CS+ exposed c) UAS-Shits1 control CS- exposed d) UAS-Shits1 control CS+ exposed e) R58E02-GAL4;; UAS-Shits1 CS- exposed f) R58E02-GAL4;; UAS-Shits1 CS+ exposed
6 6 6 6 6 6
na na na na na na
unpaired t-test for comparision between same genotype but different treatment, ANOVA (CS-) = 0.1770, ANOVA (CS+) ANOVA with Tukey's multiple comparisons = 0.9490, unpaired t-test: a vs b = test for comparision between different 0.0003, c vs d = 0.0012, e vs f = 0.02 genotype, same treatment
Figure S1E
a) R58E02-GAL4 CS+ exposed c) UAS-shits CS+ exposed e) R58E02-GAL4;; UAS-Shits1 CS+ exposed
9 9 9
yes yes yes
ANOVA with Tukey's multiple comparisons test
ANOVA = 0.411
Figure S2A
a) VT1211-GAL4 c) UAS-Shits1 b) VT1211-GAL4
9 9 9
yes yes yes
ANOVA with Tukey's multiple comparisons test for comparision
ANOVA = 0.3359
a) VT1211-GAL4 control CS- exposed c) UAS-Shits1 control CS- exposed b) VT1211-GAL4 control CS- exposed
11 11 10
yes yes yes
ANOVA with Tukey's multiple comparisons test
ANOVA = 0.5785
d) VT1211-GAL4 control CS+ exposed e) UAS-Shits1 control CS+ exposed f) VT1211-GAL4 control CS+ exposed
11 11 10
yes no yes
ANOVA with Tukey's multiple comparisons test for comparision
ANOVA = 0.6085
Figure S3A Figure S3B Figure S3C Figure S3D Figure S3E Figure S3F Figure S3G Figure S3H Figure S3I Figure S3J Figure S3K Figure S3L Figure S3M Figure S3N Figure S3O Figure S3P Figure S3Q Figure 3R
R66C08-GAL4 dendrites training no extinction OCT as CS+ R66C08-GAL4 dendrites training extinction OCT as CS+ MB112C-GAL4 dendrites training no extinction OCT as CS+ MB112C-GAL4 dendrites training extinction OCT as CS+ VT1211-GAL4 dendrites training no extinction MCH as CS+ VT1211-GAL4 dendrites training extinction MCH as CS+ VT1211-GAL4 dendrites training no extinction OCT as CS+ VT1211-GAL4 dendrites training extinction OCT as CS+ R39A05-GAL4 dendrites training no extinction OCT as CS+ R39A05-GAL4 dendrites training extinction OCT as CS+ MB112C-GAL4 dendrites mock no extinction OCT as CS+ MB112C-GAL4 dendrites mock extinction OCT as CS+ MB112C-GAL4 dendrites mock no extinction MCH as CS+ MB112C-GAL4 dendrites mock extinction MCH as CS+ VT1211-GAL4 dendrites mock no extinction MCH as CS+ VT1211-GAL4 dendrites mock extinction MCH as CS+ VT1211-GAL4 dendrites mock no extinction OCT as CS+ VT1211-GAL4 dendrites mock extinction OCT as CS+
11 13 17 15 14 13 14 11 16 12 11 12 18 15 11 11 11 10
yes yes yes no no no yes yes yes yes no no no no no no yes no
paired t-test paired t-test paired t-test Wilcoxon matched-pairs signed rank test Wilcoxon matched-pairs signed rank test Wilcoxon matched-pairs signed rank test paired t-test paired t-test paired t-test paired t-test Wilcoxon matched-pairs signed rank test Wilcoxon matched-pairs signed rank test Wilcoxon matched-pairs signed rank test Wilcoxon matched-pairs signed rank test Wilcoxon matched-pairs signed rank test Wilcoxon matched-pairs signed rank test paired t-test Wilcoxon matched-pairs signed rank test
p=0.9359 p=0.0417 p=0.0107 p=0.0302 p=0.3258 p=0.0215 p=0.3803 p=0.0104 p=0.1284 p=0.0934 p>0.999 p=0.7334 p=0.7987 p=0.9341 p=0.7002 P=0.8984 p=0.8764 p=0.7695
Figure S6A Figure S6B Figure S6C Figure S6D Figure S6E Figure S6F Figure S6G Figure S6H Figure S6I Figure S6J
VT1211-GAL4 axons training no extinction OCT as CS+ VT1211-GAL4 axons training extinction OCT as CS+ R66C08-GAL4 axons training no extinction OCT as CS+ R66C08-GAL4 axons training extinction OCT as CS+ R39A05-GAL4 axons training no extinction OCT as CS+ R39A05-GAL4 axons training extinction OCT as CS+ VT1211-GAL4 axons mock no extinction MCH as CS+ VT1211-GAL4 axons mock extinction MCH as CS+ VT1211-GAL4 axons mock no extinction OCT as CS+ VT1211-GAL4 axons mock no extinction OCT as CS+
11 10 24 12 14 16 12 11 10 11
yes yes no no yes no no yes no yes
paired t-test paired t-test Wilcoxon matched-pairs signed rank test Wilcoxon matched-pairs signed rank test paired t-test Wilcoxon matched-pairs signed rank test Wilcoxon matched-pairs signed rank test paired t-test Wilcoxon matched-pairs signed rank test paired t-test
p=0.0065 p=0.7695 p=0.0457 p=0.9697 p=0.0378 p=0.0250 p=0.1099 p=0.2848 p=0.7695 p=0.1923
Figure 2 A and B Figure 2 C Figure 2 D Figure 2 E
Figure 2 F
Figure S1B
Figure S1C left
Figure S1C right
Figure S2B
One sample t test (theoretical mean = 0)
p = 0.843
One sample t test (theoretical mean = 0)
t-test: a = 0.883, b = 0.290, c = 0.22, ANOVA: p = 0.675
paired t-test
p = 0.4852
ANOVA with Tukey's multiple comparisons test
ANOVA =0.7637
