Interaction of the Echinocandin Caspofungin with Amphotericin B or Voriconazole against Aspergillus Biofilms In Vitro Weixia Liu, Lijuan Li, Yi Sun, Wei Chen, Zhe Wan, Ruoyu Li, and Wei Liu Department of Dermatology and Venereology, Peking University First Hospital, Research Center for Medical Mycology, Peking University, Beijing, People’s Republic of China
Aspergillus biofilms were prepared from 22 strains of Aspergillus spp. via a 96-well plate-based method. Using a broth microdilution checkerboard technique with the XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] colorimetric assay, we demonstrated a synergistic antifungal activity against 18 of 22 Aspergillus biofilm strains with a combination of caspofungin and amphotericin B and against 13 of 22 strains with a combination of caspofungin and voriconazole. We did not observe antagonism.
A
spergilloma is increasing in incidence (2, 9). The infection is most often seen in tuberculosis cavities or in the maxillary sinus. Histopathological examination reveals that aspergilloma is an organized structure of fungal filaments, which is enveloped by an extracellular matrix without any infiltration of host cells (9). This is similar to the structure of a biofilm (4, 16, 17), and thus, it is believed to be a type of biofilm (9). In vitro studies (5, 11, 12) showed that Aspergillus spp. can form biofilms, which exhibited a significantly decreased susceptibility to antifungal drugs compared to the planktonic cells of Aspergillus spp. Therefore, we asked whether the combination of caspofungin (CAS) with amphotericin B (AMB) or voriconazole (VRC), which are also known to have synergistic inhibitory activity against planktonic cells of Aspergillus spp. (1, 6, 7, 10), could have a synergistic inhibitory activity against Aspergillus biofilms. (This work was presented in part at the 18th Congress of the International Society for Human and Animal Mycology, Berlin, Germany, 11 to 15 June 2012 [8a].) In the present study, Aspergillus biofilms were prepared from 22 strains of Aspergillus spp., including 11 strains of Aspergillus fumigatus, 5 strains of Aspergillus flavus, 3 strains of Aspergillus terreus, and 3 strains of Aspergillus niger, by using a 96-well platebased method (14). All strains were stored at the Research Center for Medical Mycology of Beijing University and were subcultured on Sabouraud dextrose agar at 35°C for 3 days to ensure purity and viability. Using a broth microdilution checkerboard assay and XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium5-carboxanilide] colorimetric measurement (14, 18), we tested the interaction of CAS with VRC or AMB against the Aspergillus biofilms of these 22 strains. All antifungal drugs were provided as standard powders. VRC (Shouguang Pharmaceutical, Shan Dong, China) and AMB (Sigma Chemical Co., St. Louis, MO) were dissolved in 100% dimethyl sulfoxide (DMSO), while CAS (Merck & Co., Inc., Rahway, NJ) was dissolved in sterile water. The ranges of working concentrations of CAS, VRC, and AMB were 2 to 256 g/ml, 0.5 to 256 g/ml, and 0.06 to 32 g/ml, respectively. XTT powder (Sigma Chemical Co., St. Louis, MO) was prepared as a saturated solution at 500 g/ml in phosphatebuffered saline (PBS), and menadione (vitamin K3, Sigma Chemical Co., St. Louis, MO) was dissolved in 100% acetone at a concentration of 10 mmol/liter. Both solutions were stored at ⫺70°C. When used, 1 l of the stock solution of menadione was added to
6414
aac.asm.org
Antimicrobial Agents and Chemotherapy
10 ml XTT solution to achieve a final menadione concentration of 1 mol/liter. As described previously (11, 14), the sessile MIC50 (SMIC50) was assayed to evaluate the antifungal activity of the drugs against Aspergillus biofilms. The interaction of CAS with AMB or VRC was determined and was classified on the basis of the fractional inhibitory concentration index (FICI), defined as follows (13, 18): FICI ⱕ 0.5, synergy; 0.5 ⬍ FICI ⱕ 4, no interaction; FICI ⬎ 4, antagonism. At the same time, the interaction of CAS with AMB or VRC against planktonic cells of all strains of Aspergillus spp. was also evaluated as described previously (1, 3). The minimum effective concentration (MEC) of CAS, when combined with VRC and AMB, and the corresponding drug concentration of VRC and AMB were also recorded. We observed that the combination of CAS with AMB had synergistic inhibitory activity against 8 of 11 A. fumigatus biofilm strains with FICI values of 0.19 to 0.5, and 10 of 11 non-fumigatus Aspergillus biofilm strains, with FICI values of 0.13 to 0.38 (Table 1). As for the combination of CAS and VRC, synergistic inhibitory activity was also observed in 7 of 11 A. fumigatus biofilms, with FICI values of 0.188 to 0.5, and in 6 of 11 non-fumigatus Aspergillus biofilms, with FICI values of 0.27 to 0.5 (Table 1). No antagonistic effects were observed. In addition, the percentage of metabolic activity in A. fumigatus AF293 biofilm treated with a combination of CAS and AMB or VRC was calculated (XTT assay). We demonstrate that the combination of CAS and AMB or VRC significantly reduced the metabolic activity of A. fumigatus AF293 biofilm in comparison to CAS, AMB, or VRC alone at the same concentrations (Fig. 1). However, no interaction was observed in these combinations of antifungal drugs against planktonic cells (Table 1). CAS at 4 g/ml (CAS1) was synergistic with AMB (0.25 g/ ml), and CAS at 8 g/ml (CAS2) was synergistic with VRC (4
Received 30 March 2012 Returned for modification 20 June 2012 Accepted 22 September 2012 Published ahead of print 1 October 2012 Address correspondence to Wei Liu,
[email protected]. Weixia Liu and Lijuan Li contributed equally to this article. Supplemental material for this article may be found at http://aac.asm.org/. Copyright © 2012, American Society for Microbiology. All Rights Reserved. doi:10.1128/AAC.00687-12
p. 6414 – 6416
December 2012 Volume 56 Number 12
Combination of Antifungals against Aspergillus Biofilm
TABLE 1 Interaction of CAS with AMB or VRC against Aspergillus biofilms and planktonic cells of 22 strains of Aspergillus spp. SMIC50 (g/ml)a
SMIC50 (g/ml)
MIC (g/ml)b
MIC (g/ml)
MEC (g/ml)c
Strain
CAS
VRC
CAS/VRC FICI
AMB CAS/AMB FICI
CAS VRC
CAS/VRC FICI
AMB CAS/AMB FICI
CAS
CAS/VRCd
CAS/AMBd
A. fumigatus AF293 AFBMU01340 AFBMU04718 AFBMU04728 AFBMU04736 AFBMU04737 AFBMU04748 AFBMU01200 AFBMU02731 AFBMU02810 AFBMU04787
32 64 32 32 64 64 32 64 64 64 32
32 8 8 4 4 8 8 8 8 8 8
8/4 4/1 4/1 4/0.25 32/2 64/4 4/2 64/2 16/2 16/4 2/2
0.375 0.188 0.25 0.188 1 1.5 0.375 1.25 0.5 0.75 0.313
4 8 2 4 8 2 4 2 2 4 2
4/0.25 16/2 4/0.25 4/1 4/0.25 32/1 4/1 8/0.5 16/0.25 4/4 32/0.25
0.188 0.5 0.25 0.375 0.094 1 0.375 0.375 0.375 1.063 1.13
ⱖ16 ⱖ16 ⱖ16 ⱖ16 ⱖ16 ⱖ16 ⱖ16 ⱖ16 ⱖ16 ⱖ16 ⱖ16
0.25 0.25 0.25 0.25 0.25 0.25 0.5 0.25 0.25 0.25 4
0.03/0.25 0.03/0.25 0.03/0.25 0.03/0.25 0.03/0.25 8/0.125 16/0.25 16/0.25 0.03/0.25 0.03/0.25 16/2
1.001 1.001 1.001 1.001 1.001 0.75 1 1.5 1.001 1.001 1
0.25 0.25 0.5 1 1 1 0.5 0.5 0.25 0.25 0.5
0.03/0.25 0.03/0.25 2/0.25 0.03/1 0.03/1 0.125/0.5 0.03/0.5 0.03/0.5 16/0.125 16/0.125 16/0.125
1.001 1.001 0.563 1.001 1.001 0.504 1.001 1.001 1 1 0.75
0.125 0.125 0.125 0.125 0.125 0.125 0.125 0.125 0.125 0.06 0.125
0.03/0.125 0.03/0.06 0.03/0.06 0.03/0.06 0.03/0.125 ⱕ0.03/0.125 0.03/0.125 0.03/0.06 0.125/0.125 0.03/0.125 0.03/1
0.06/0.06 0.03/0.125 0.06/0.25 0.03/0.25 0.06/0.25 0.03/0.25 0.03/0.25 0.06/0.125 0.06/0.125 0.03/0.125 0.03/0.25
A. flavus AFLBMU03932 AFLBMU03035 AFLBMU00969 AFLBMU03142 AFLBMU29791
256 64 ⬎256 64 64
8 2 ⬎256 256 64
4/2 4/0.5 128/128 64/256 16/16
0.266 0.266 0.5 2 0.5
4 2 ⬎32 16 ⬎32
8/0.5 2/0.25 4/4 16/1 2/4
0.156 0.156 0.07 0.313 0.094
ⱖ16 ⱖ16 ⱖ16 ⱖ16 ⱖ16
0.25 0.25 0.25 0.25 4
0.03/0.25 0.03/0.25 0.03/0.25 0.03/0.25 0.03/4
1.001 1.001 1.001 1.001 1.001
1 0.5 0.5 0.5 0.5
0.03/1 0.03/0.5 0.03/0.5 0.03/0.5 0.03/0.5
1.001 1.001 1.001 1.001 1.001
0.125 0.125 0.125 0.125 0.125
0.03/0.125 0.06/0.06 0.03/0.125 0.06/0.125 0.03/0.5
0.06/0.125 0.03/0.125 0.03/0.25 0.03/0.25 0.03/0.25
A. terreus ATBMU00802 ATBMU01017 ATBMU04033
64 32 256
256 64 128
32/16 32/8 256/0.5
0.563 ⬎32 1.125 8 1.004 32
8/1 4/1 128/32
0.141 ⱖ16 0.25 0.03/0.25 1.001 1 0.25 ⱖ16 0.125 0.03/0.125 1.001 1 1.5 ⱖ16 0.5 0.03/0.5 1.001 1
0.03/1 0.03/1 0.03/1
1.001 0.125 0.03/0.125 1.001 0.06 0.06/0.06 1.001 0.125 0.03/0.125
0.125/0.06 0.03/0.25 0.03/0.5
A. niger ANBMU04689 ANBMU04778 ANBMU04646
64 64 64
256 32 32
4/128 16/8 8/8
0.563 2 0.5 2 0.375 4
4/0.5 8/0.25 4/1
0.313 ⱖ16 0.125 0.03/0.125 1.001 0.5 0.25 ⱖ16 0.25 0.03/0.25 1.001 0.5 0.313 ⱖ16 0.25 0.03/0.25 1.001 0.25
0.03/0.5 1.001 0.125 0.03/0.25 0.03/0.25 0.501 0.125 ⱕ0.03/0.25 0.03/0.125 0.501 0.125 0.03/0.25
0.125/0.06 0.03/0.125 ⱕ0.03/0.125
a
SMIC50, the sessile MIC, the concentration at which a 50% decrease in absorbance was detected in comparison to the absorbance of an untreated biofilm formed by the same fungal strains. b MIC at which no fungal growth was observed with the naked eye. c MEC, the minimum effective concentration at which the stubby, aberrant fungal filaments was observed, compared to the untreated organism. d The MEC of CAS and the corresponding concentration of VRC or AMB at which stubby, aberrant growth of fungal filaments was observed when CAS was combined with VRC or AMB. No FICI could be calculated because the MIC, instead of the MEC, is used to describe the antifungal activity of VRC or AMB alone against Aspergillus spp.
g/ml) against a biofilm of A. fumigatus AF293 (Table 1). Therefore, the combination of CAS1 (4 g/ml) with AMB (0.25 g/ml), as well as that of CAS2 (8 g/ml) with VRC (4 g/ml) was used to inhibit the biofilm of A. fumigatus AF293. This combination was used as a model to observe the synergistic activity against Aspergillus biofilm by FUN-1 staining (8, 15). With an Olympus FV-500 laser scanning confocal microscope, we observed that the amount
of viable organisms in the A. fumigatus AF293 biofilm was significantly less when being treated with these drugs—CAS1 (4 g/ml) with AMB (0.25 g/ml) or CAS2 (8 g/ml) with VRC (4 g/ml) (see Fig. S1 and S2 in the supplemental material). Again, these results suggest a synergistic activity of CAS with AMB or VRC against Aspergillus biofilm. ACKNOWLEDGMENTS This work was supported by the Program for New Century Excellent Talents in University NCET-10-0198 (Wei Liu) and BMU20110158 (Wei Liu) and by grants from National Natural Science Foundation of China 30970131 (Wei Liu) and 30930006 (Ruoyu Li). This study was also supported by grant 7102149 from the Beijing Natural Science Foundation (Wei Liu). The funders had no role in study design, data analysis, decision to publish, or preparation of the manuscript. We thank Richard Calderone, Georgetown University, Washington, DC, for critical reading of the manuscript. This study does not present any conflicts of interest for us.
REFERENCES
FIG 1 Inhibitory activity (XTT assays) of CAS with AMB or VRC against A. fumigatus AF293 biofilm. The metabolic activity of A. fumigatus AF293 biofilm was 97% when treated with CAS1 (4 g/ml) or AMB (0.25 g/ml) alone, but it was 49% when treated with CAS1 (4 g/ml) and AMB (0.25 g/ml). The metabolic activities of A. fumigatus AF293 biofilm were 76% and 68% when treated with CAS2 (8 g/ml) or VRC (4 g/ml) alone, but the activity was 48% when treated with a combination of CAS2 (8 g/ml) and VRC (4 g/ml).
December 2012 Volume 56 Number 12
1. Arikan S, Lozano-Chiu M, Paetznick V, Rex JH. 2002. In vitro synergy of caspofungin and amphotericin B against Aspergillus and Fusarium spp. Antimicrob. Agents Chemother. 46:245–247. 2. Beauvais A, et al. 2007. An extracellular matrix glues together the aerialgrown hyphae of Aspergillus fumigatus. Cell. Microbiol. 9:1588 –1600. 3. Clinical and Laboratory Standards Institute. 2008. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi. Approved standard M38-A2. CLSI, Wayne, PA. 4. Costerton JW, Lewandowski Z, Caldwell DE, Korber DR, Lappin-Scott HM. 1995. Microbial biofilms. Annu. Rev. Microbiol. 49:711–745. 5. Fiori B, et al. 2011. In vitro activity of anidulafungin and other antifungal
aac.asm.org 6415
Liu et al.
agents against biofilms formed by clinical isolates of different Candida and Aspergillus species. Antimicrob. Agents Chemother. 55:3031–3035. 6. Kirkpatrick WR, Perea S, Coco BJ, Patterson TF. 2002. Efficacy of caspofungin alone and in combination with voriconazole in a Guinea pig model of invasive aspergillosis. Antimicrob. Agents Chemother. 46:2564 – 2568. 7. Kontoyiannis DP, et al. 2003. Efficacy and toxicity of caspofungin in combination with liposomal amphotericin B as primary or salvage treatment of invasive aspergillosis in patients with hematologic malignancies. Cancer 98:292–299. 8. Liu W, et al. 2003. Attenuation of itraconazole fungicidal activity following preexposure of Aspergillus fumigatus to fluconazole. Antimicrob. Agents Chemother. 47:3592–3597. 8a.Liu, W, et al. 2012. Abstr. 18th Congr. Int. Soc. Hum. Anim. Mycol., Berlin, Germany, 11 to 15 June 2012, abstr P761. 9. Loussert C, et al. 2010. In vivo biofilm composition of Aspergillus fumigatus. Cell. Microbiol. 12:405– 410. 10. Lum LR, Turco TF, Leone J. 2002. Combination therapy with caspofungin and amphotericin B lipid complex. Am. J. Health Syst. Pharm. 59: 80 – 81. 11. Mowat E, Butcher J, Lang S, Williams C, Ramage G. 2007. Development of a simple model for studying the effects of antifungal agents on multi-
6416
aac.asm.org
12. 13. 14. 15.
16. 17. 18.
cellular communities of Aspergillus fumigatus. J. Med. Microbiol. 56: 1205–1212. Mowat E, et al. 2008. Phase-dependent antifungal activity against Aspergillus fumigatus developing multicellular filamentous biofilms. J. Antimicrob. Chemother. 62:1281–1284. Odds FC. 2003. Synergy, antagonism, and what the chequerboard puts between them. J. Antimicrob. Chemother. 52:1. doi:10.1093/jac/dkg301. Pierce CG, et al. 2008. A simple and reproducible 96-well plate-based method for the formation of fungal biofilms and its application to antifungal susceptibility testing. Nat. Protoc. 3:1494 –1500. Pina-Vaz C, Rodrigues AG, Costa-de-Oliveira S, Ricardo E, Mardh PA. 2005. Potent synergic effect between ibuprofen and azoles on Candida resulting from blockade of efflux pumps as determined by FUN-1 staining and flow cytometry. J. Antimicrob. Chemother. 56:678 – 685. Ramage G, Vandewalle K, Wickes BL, López-Ribot JL. 2001. Characteristics of biofilm formation by Candida albicans. Rev. Iberoam. Micol. 18:163–170. Ramage G, Saville SP, Thomas DP, López-Ribot JL. 2005. Candida biofilms: an update. Eukaryot. Cell 4:633– 638. Tobudic S, Kratzer C, Lassnigg A, Graninger W, Presterl E. 2010. In vitro activity of antifungal combination against Candida albicans biofilms. J. Antimicrob. Chemother. 65:271–274.
Antimicrobial Agents and Chemotherapy