Introduction miRNA TOOLS miRNA Databases ...

Athar Hussain1, Ghulam Mustafa1 , Sarfaraz Hussain1 , Aqsa Mushtaq1

Introduction A micro RNA (abbreviated miRNA) is a small non-coding RNAmolecule (containing about 22 nucleotides) found in plants, animals, and some viruses, which functions in RNA silencing and posttranscriptional regulation of gene expression Encoded by eukaryotic nuclear DNA in plants and animals and by viral DNA in certain viruses whose genome is based on DNA, miRNAs function via base-pairing with complementary sequences within mRNA molecules. As a result, these mRNA molecules aresilenced by one or more of the following processes: 1) cleavage of the mRNA strand into two pieces, 2) destabilization of the mRNA through shortening of its poly (A) tail, and 3) less efficient translationof the mRNA into proteins by ribosomes , miRNAs resemble thesmall interfering RNAs (siRNAs) of the RNA interference (RNAi)pathway, except miRNAs derive from regions of RNA transcripts that fold back on themselves to form short hairpins, whereas siRNAs derive from longer regions of double-stranded RNA .The mature miRNA is part of an active RNA-induced silencing complex (RISC) containing Dicer and many associated proteins. RISC is also known as a microRNA ribonucleoprotein complex (miRNP); RISC with incorporated miRNA is sometimes referred to as "miRISC”[1].

miRNA TOOLS

Mechanisms Processing of incoming RNA

There are Three main Types Of miRNA TOOLS

Capture & Degradation of mRNA

Assembly of RISC

1.miRNA Deep Sequencing Tools[2]: i)miRTools ii)miRDeep deepBase iV)miRExpress V)miRAnalyzer Vi)CID-miRNA Vii)miR-Cat

Processing of pre-miRNA to miRNA

Production of pre-miRNA

iii)

Capture of mRNA and Suppression

2.miRNA target prediction[2]: software: a)PITA b)Diana c) Micro-T d) MirPath e)Microinspector f)MaMi g)miRecords h)mirDIP i)miRGator j)Magia k)Targetscan l) miRTar m) miRTrail

3.Plant miRNA targets[2]: a)psRNA d) SoMART

b) target c) TAPIR e)TargetAling

miRNA Databases

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Methodology Gene Sequence Retrieved from Databases,NCBI,Genbank,DDBj

Bioinformatics Tools

Selection based Target score

Result/Output

Acknowledgement

Outputs 1

Department Of Bioinformatics &

Biotechnology , Govt. College University Faisalabad

References [1]https://en.wikipedia.org/wiki/MicroRNA [2]https://sites.google.com/site/mirnatools/ [3]https://sites.google.com/site/mirnatools/mirna-databases