Jan 18, 2014 - ... agriculture, biotechnology, enzymes, rhizosphere. 1Philippine Rice Research Institute, Science City of Muñoz, Nueva Ecija, Philippines.
ASIA LIFE SCIENCES 23(2): 413-427, 2014
The Asian International Journal of Life Sciences
Isolation and screening of Actinomycetes for its growth promoting activities
JAYVEE A. CRUZ1* and ERLINDA S. PATERNO2, 3
Screening of actinomycetes examined its growth promoting activities (GPA) as potential microbial inoculant which include indoleacetic acid (IAA) production, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity, and phosphate solubilization. Fifty-nine isolates from rice and corn rhizospheres and existing collection of the National Institute of Molecular Biology and Biotechnology (BIOTECH) were screened for growth promoting activities. Thirty-two isolates produced indoleacetic acid (IAA), 14 of which showed ACC deaminase activity while 24 solubilized bound phosphate in vitro. Five isolates were selected on the basis of their growth promoting activities such as their IAA and ACC deaminase production. Growth rate was also considered as basis of selection. Isolates that were able to grow within 3 to 4 days were selected. Keywords: Actinomycetes, agriculture, biotechnology, enzymes, rhizosphere
Philippine Rice Research Institute, Science City of Muñoz, Nueva Ecija, Philippines National Institute of Molecular Biology and Biotechnology (BIOTECH), University of the Philippines Los Baños, College 4031, Laguna, Philippines. 3 Agricultural Systems Cluster (ASC), College of Agriculture, University of the Philippines Los Baños, College 4031, Laguna, Philippines *Corresponding author 1 2
