May 5, 2011 - i fili f i. [Adv Exp Med Biol. 2007] · Review Gene expression profiling of primary · tumor cell populations using laser capture i di ti RNA t. i t lifi ti.
Laser capture microdissection of hepatic stages of... [Methods Mol Biol. 2005] - PubMed result Resources
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Related citations Laser capture microdissection of Plasmodium falciparum liver stages mRNA Parasitol. analysis. 2002] [Molfor Biochem
Methods Mol Biol. 2005;293:301-7.
Laser capture microdissection of hepatic stages of the human parasite Plasmodium falciparum for molecular analysis. Semblat JP, Silvie O, Franetich JF, Mazier D. Immunobiologie Cellulaire et Moléculaire des Infections Parasitaires, Centre Hospitalier-Universitaire PitiéSalpêtriere, Université Pierre et Marie Curie, Paris, France.
Abstract Despite the sequencing of parasite genomes and development of DNA microarray technology, gene profiling of parasites remains a difficult task. For example, transcriptome analysis cannot currently be applied to the hepatic stages of the malaria parasite Plasmodium falciparum due to difficulties in obtaining sufficient amounts of parasite material that lies among the large excess of host cell RNA. Here, we describe the isolation of P. falciparum-infected human hepatocytes by a laser capture microdissection approach. Reverse transcriptase polymerase chain reaction amplification of several P. falciparum transcripts demonstrates the high quality of the RNA recovered after microdissection. This approach should enable analysis of P. falciparum transcriptome during its hepatic development, a major step toward the identification of new therapeutic and vaccine targets.
Temperature shift and host cell contact upregulate sporozoite expression [PLoS of Plasmodium Pathog. 2008] f l i i l di h t t Plasmodium falciparum infection and exoerythrocytic development[Int in mice J Parasitol. with 2006] hi i h li Review Complementary techniques: laser capture microdissection--increasing [Adv Exp Med specificity Biol. 2007] of i fili f i Review Gene expression profiling of primary tumor cell populations using laser capture [Methods Mol Biol. 2005] i di ti RNA t i t lifi ti See reviews... See all...
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