LIFE SCIENCES Catalogue - Diamed

LIFE SCIENCES Catalogue sciences Pg 16

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Diamed Life Sciences is proud to partner with SmoBio, GeneRay and ABM a Canadian company of 8 years, to bring you the very best reagents for your PCR, RT-PCR and qRT-PCR applications

dNTP products are available as a set or a ready to use mix. These are high quality products that are functionally tested in a variety of applications; the purity of each deoxynucleotide is ≥98% as determined by HLPC analysis. Reverse Transcriptase products are engineered to perform under high temperatures. These products can synthesize the full length of cDNA libraries from RNA.

First Strand cDNA Synthesis Kits generate high quality cDNA by including both random primers and oligo(dT) as part of a full set of reagents; Both the random primers and the oligo (dT) are included for a choice of general priming strategies and as alternatives to gene-specific primers.

ABM’s cDNA Synthesis Super Mix provides a highly efficient and specific conversion of RNA to cDNA, even from low-abundance transcripts; This product also allows you to perform first-strand cDNA synthesis with minimal effort and maximum ease. TAQ DNA Polymerase Products are supplied with buffer, are thoroughly tested and have protocols which are optimized for thermocycler conditions.

PCR Mastermix Products for PCR, RT-PCR & qRT-PCR are highly sensitive, accommodate a wide range of DNA Templates and are optimized for your thermocycler in a pre-mixed format simplifying workflow. The products described as “with dye” come complete with an inert blue dye & a stabilizer for direct loading into an agarose gel.

Pg 8

Extraction Kits provide a simple, fast and efficient method for eluting high quality DNA or RNA. Trizol Reagent is used for Total Extraction of RNA, producing results of high purity from a fast procedure.

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Genomic DNA Isolation Kits are available in three types; for Cells & Tissue, for Blood & for Bacterial Cells. These kits process samples in a time saving spin column format to produce purified high molecular weight genomic DNA which is suitable for direct use in all common molecular biology applications.

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Purification Kits are fast and efficient procedure as Bind – Wash – Elute all occur in spin column format. All of the purification kits yield high quality DNA.

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Nucleotide Stains are environmentally friendly, non-carcenogenic & non-mutagenic and are an environmentally safe alternative to Ethidium Bromide. These products are used for visualization of dsDNA, ssDNA & RNA under LED Blue & UV Light.

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DNA Ladders & Protein Markers are products that provide exceptionally sharp bands and allow you to choose from a wide range of molecular weights; The DNA Ladder products are ready-to-use and come pre-mixed with loading dye for direct loading on gel.

TM

LS-vJ17-2014

EasyScript™ Reverse Transcriptase Synthesize cDNA from only 0.1pg of RNA with abm’s EasyScript™ Reverse Transcriptase. Using strategic modifications, abm’s EasyScript™ Reverse Transcriptase lacks the intrinsic RNase H acitivity thereby drastically increasing the yield and the achievable synthesized cDNA length (up to 9kb). In addition, EasyScript™ contains a fidelity-enhancing subunit which ensures superior accuracy in reverse transcription reaction(s). With abm’s EasyScript™ Reverse Transcriptase, you can expect:          

Synthesizing cDNA from a ssRNA DNA primer extension Sequencing dsDNA Constructing cDNA library Constructing libraries for serial analysis for gene expression (SAGE) Synthesizing cDNA in rapid amplification of cDNA ends (3’ & 5’ RACE) Producing template for use in RT-PCR or real-time RT-PCR Labelling 3’-end of duplex DNA via end-filling reactions Generating probes for hybridization. Enzyme supplied with 5x RT buffer

ABMG231

EasyScript™ Reverse Transcriptase

25 reactions of 20 μl

$45.00

ABMG232

EasyScript™ Reverse Transcriptase

100 reactions of 20 μl

$95.00

EasyScript™ Plus Reverse Transcriptase Synthesize cDNA from complex RNA templates (i.e. secondary structures and high GC content) with abm’s EasyScript™ Plus Reverse Transcriptase. Engineered to perform under high temperatures (45° - 55° C), abm’s EasyScript™ Plus Reverse Transcriptase can synthesize full-length cDNA libraries from RNA templates up to 15kb in length. In addition, EasyScript Plus™ Reverse Transcriptase has outstanding proofreading ability due to the presence of a fidelity-enhancing subunit, thus making this RTase an excellent choice for whole genome sequencing. With abm’s EasyScript™ Plus Reverse Transcriptase, you can expect:           

Longer DNA primer extension up to 15kb Sequencing more complex dsDNA Active at higher temperature range (450-550C) Synthesizing cDNA from a ssRNA Constructing cDNA library Constructing libraries for serial analysis for gene expression (SAGE) Synthesizing cDNA in rapid amplification of cDNA ends (3’ & 5’ RACE) Producing template for use in RT-PCR or real-time RT-PCR Labelling 3’-end of duplex DNA via end-filling reactions Generating probes for hybridization Enzyme supplied with 5x RT buffer

ABMG177

EasyScript™ Plus Reverse Transcriptase

25 reactions of 20 μl

$75.00

ABMG237

EasyScript™ Plus Reverse Transcriptase

100 reactions of 20 μl

$145.00

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First Strand EasyScript™ cDNA Synthesis Kit Choose your priming methods with abm’s EasyScript™ cDNA Synthesis Kit. abm’s cDNA Synthesis kit provides a comprehensive set of reagents necessary to generate high quality cDNA and offers the most flexibility in respect to priming methods and reaction optimization. Both random primers and oligo(dT) are included for a choice of general priming strategies and as alternatives to gene-specific primers. This kit employs abm’s EasyScript™ Reverse Transcriptase. With abm’s EasyScript™ cDNA Synthesis Kit, you can expect:          

Maximal flexibility in priming – oligo(dT), random primers or gene-specific primers Robust cDNA synthesis from any RNA template High reproducibility and excellent yield Synthesizing cDNA from ssRNA DNA primer extension Sequencing dsDNA Constructing cDNA library Active Temperature Producing template for use in RT-PCR Labelling 3’-end of duplex DNA via end-filling reactions Generating probes for hybridization

ABMG233

EasyScript™ cDNA Synthesis Kit

25 reactions of 20 μl

$75.00

ABMG234

EasyScript™ cDNA Synthesis Kit

100 reactions of 20 μl

$105.00

420C

First Strand EasyScript™ Plus cDNA Synthesis Kit Choose your priming methods with abm’s EasyScript™ Plus cDNA Synthesis Kit. abm’s cDNA Synthesis kit provides a comprehensive set of reagents necessary to generate high quality cDNA and offers the most flexibility in respect to priming methods and reaction optimization. Both random primers and oligo(dT) are included for a choice of general priming strategies and as alternatives to gene-specific primers.

This kit employs abm’s EasyScript™ Plus Reverse Transcriptase. With abm’s EasyScript™ Plus cDNA Synthesis Kit, you can expect:          

Maximal flexibility in priming – oligo(dT), random primers or gene-specific primers Robust cDNA synthesis from any RNA template High reproducibility and excellent yield Synthesizing cDNA from ssRNA DNA primer extension Sequencing dsDNA Constructing cDNA library Active Temperature Producing template for use in RT-PCR Labelling 3’-end of duplex DNA via end-filling reactions Generating probes for hybridization

ABMG235

EasyScript™ Plus cDNA Synthesis Kit

25 reactions of 20 μl

$100.00

ABMG236

EasyScript™ Plus cDNA Synthesis Kit

100 reactions of 20 μl

$155.00

450-550C

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First Strand EasyScript™ cDNA Synthesis SuperMix Convenient and time-saving reaction set-up with abm’s EasyScript™ cDNA Synthesis SuperMix. abm’s cDNA Synthesis SuperMix provides highly efficient and specific conversion of RNA to cDNA, even from low-abundance transcripts. Furthermore, the unique blend of random primers and oligo(dT) primers in this SuperMix ensures optimal representation of all transcript sequences in the cDNA product. Overall, abm’s cDNA Synthesis SuperMix allows you to perform first-strand cDNA synthesis with minimal effort and maximum ease. This SuperMix employs abm’s EasyScript™ Reverse Transcriptase. (Pg 2) With abm’s EasyScript™ cDNA Synthesis SuperMix, you can expect:         

Streamlined protocol suitable for high-throughput applications Ease of use with a simple set-up Excellent cDNA yield Generation of templates for use in RT-PCR and qRT-PCR cDNA synthesis from ssRNA cDNA library construction Generation of probes for hybridization DNA primer extension RTase is in storage buffer containing 50 % glycerol

Active Temperature

ABMG451

EasyScript™ cDNA Synthesis SuperMix

25 reactions of 20 μl

$50.00

ABMG452

EasyScript™ cDNA Synthesis SuperMix

100 reactions of 20 μl

$175.00

420C

First Strand EasyScript™ Plus cDNA Synthesis SuperMix Convenient and time-saving reaction set-up with abm’s EasyScript™ Plus cDNA Synthesis SuperMix. abm’s cDNA Synthesis SuperMix provides highly efficient and specific conversion of RNA to cDNA, even from low-abundance transcripts. Furthermore, the unique blend of random primers and oligo(dT) primers in this SuperMix ensures optimal representation of all transcript sequences in the cDNA product. Overall, abm’s cDNA Synthesis SuperMix allows you to perform first-strand cDNA synthesis with minimal effort and maximum ease. This SuperMix employs abm’s EasyScript™ Plus Reverse Transcriptase. (Pg 2) With abm’s EasyScript™ Plus cDNA Synthesis SuperMix, you can expect:         

Streamlined protocol suitable for high-throughput applications Ease of use with a simple set-up Excellent cDNA yield Generation of templates for use in RT-PCR and qRT-PCR cDNA synthesis from ssRNA cDNA library construction Generation of probes for hybridization DNA primer extension RTase is in storage buffer containing 50 % glycerol

Active Temperature

ABMG453

EasyScript™ Plus cDNA Synthesis SuperMix

25 reactions of 20 μl

$70.00

ABMG454

EasyScript™ Plus cDNA Synthesis SuperMix

100 reactions of 20 μl

$265.00

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450-550C

Taq DNA Polymerase Routine amplification in a cost-effective way? abm’s Taq DNA Polymerase is the answer. abm’s Taq DNA polymerase offers consistent results across a wide range of DNA templates with excellent yield and sensitivity. This conventional polymerase is most suitable for PCR applications where less emphasis is placed on fidelity. With abm’s Taq DNA Polymerase, you can expect:  Robust PCR performance with great reproducibility  Suitable for a wide range of PCR assays, TA cloning & routine PCR amplification of DNA templates up to 6 kb  High sensitivity ABMG009

DNA Polymerase

200 μl (1,000 U)

$50.00

ABMG008

DNA Polymerase

1.0 ml (5,000 U)

$175.00

ABMG126

DNA Polymerase

2 x 1.0 ml (10,000 U)

$285.00

ABMG013

2x PCR Mastermix

5.0 ml (200 rxns)

$125.00

ABMG013-dye

2x PCR Mastermix with dye

5.0 ml (200 rxns)

$125.00

Bestaq™ DNA Polymerase Looking to improve? Bestaq™ DNA Polymerase is the new standard. With outstanding PCR yield, exceptional fidelity and high processivity; this versatile enzyme is ideal for all PCR applications. With this superior enzyme, you can consolidate all PCR protocols and reactions into one efficient system. With abm’s Bestaq™ DNA Polymerase, you can expect:     

High-speed PCR without compromising accuracy High-processivity to reduce reaction time by 70% Robust and high yield across a wide range of templates Efficient amplification of DNA templates up to 15kb Designed for High-throughput PCR, RACE & NGS Library construction through robust amplification of AT- and GC-Rich sequences

ABMG456

DNA Polymerase

50 μl (250 U)

$65.00

ABMG457

DNA Polymerase

200 μl (1,000 U)

$230.00

ABMG464

2x PCR Mastermix

5.0 ml (200 rxns)

$195.00

ABMG464-dye

2x PCR Mastermix with dye

5.0 ml (200 rxns)

$195.00

Taq Plus DNA Polymerase Looking for Cost-effective quick PCR with improved fidelity? Use abm’s Taq Plus DNA Polymerase. abm’s Taq Plus DNA polymerase employs an optimized two-polymerase blend that provides high product yield, sensitivity and fidelity. Taq Plus DNA Polymerase can be used in a variety of general PCR experiments, providing improved efficiency and performance at a reduced cost. With abm’s Taq Plus DNA Polymerase, you can expect:    

Improved sensitivity and fidelity compared to conventional Taq DNA Polymerase Robust and consistent performance across a wide range of templates An economical alternative to Taq DNA Polymerase Suitable for a wide range of PCR assays, routine PCR amplification of DNA templates up to 6 kb & TA cloning

ABMG012

DNA Polymerase

50 μl (250 U)

$45.00

ABMG040

DNA Polymerase

200 μl (1,000 U)

$130.00

ABMG014

2x PCR Mastermix

5.0 ml (200 rxns)

$145.00

ABMG014-dye

2x PCR Mastermix with dye

5.0 ml (200 rxns)

$145.00

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Precision™ DNA Polymerase Are you frustrated with amplification errors? Let us introduce you to abm’s Precision™ DNA Polymerase. Significant time and effort can be saved by using high fidelity polymerases that eliminates the need for downstream error-correction steps. The use of Precision™ DNA Polymerase for rare DNA templates is imperative as small amounts of DNA are especially prone to high mutant frequencies. abm’s Precision™ DNA Polymerase is the ideal choice for applications demanding high fidelity PCR products. With abm’s Precision™ DNA Polymerase, you can expect:  Outstanding accuracy and efficiency through Error-free PCR products for downstream processes  A product designed for whole genome sequencing, Site-directed mutagenesis, blunt-end cloning, as well as, specific amplification of difficult templates (i.e. GC-Rich) & low copy PCR assays ABMG078

DNA Polymerase

100 μl (500 U)

$160.00

ABMG124

2x PCR Mastermix

5.0 ml (200 rxns)

$125.00

ABMG124-dye

2x PCR Mastermix with dye

5.0 ml (200 rxns)

$125.00

HotStart DNA Polymerase (PCR) Are you experiencing problems with non-specific amplification and primer-dimer formation? Try abm’s HotStart DNA Polymerase. HotStart DNA Polymerase is chemically modified such that the Taq enzyme is inactivated by a heat-labile group at room temperature. This feature significantly reduces non-specific product formations that would otherwise compete for reagent availability. Thus, abm’s HotStart DNA Polymerase offers improved yield of desired PCR products. With abm’s HotStart DNA Polymerase, you can achieve:     

The highest specificity with minimal background Superior performance Improved yield of desired product Assays with prolonged reaction setup or liquid handling Success with a product designed for multiplex PCR, specific amplification of difficult templates (i.e. G-C rich), low copy PCR assays & TA Cloning

ABMG011

DNA Polymerase

50 μl (250 U)

$72.50

ABMG039

DNA Polymerase

200 μl (1,000 U)

$125.00

TaqFast DNA Polymerase Short on time? Send abm’s TaqFast DNA Polymerase to the rescue. With extension rate as fast as 10 sec/kb (compared to 60 sec/kb with regular Taq), abm’s TaqFast DNA polymerase offers the ultimate speed to dramatically reduce total reaction time. In addition to improved processivity, TaqFast DNA polymerase also possesses moderate 3’-5’ proofreading activity, making this enzyme well suited for high-throughput PCR. With abm’s TaqFast DNA Polymerase, you can expect:  High-speed PCR without compromising accuracy  High-processivity to reduce reaction time by 80%  Designed for Fast PCR & High-throughput PCR

ABMG277

DNA Polymerase

50 μl (250 U)

$72.50

ABMG278

DNA Polymerase

200 μl (1,000 U)

$210.00

ABMG280

2x PCR Mastermix

5.0 ml (200 rxns)

$100.00

ABMG280-dye

2x PCR Mastermix with dye

5.0 ml (200 rxns)

$100.00

Bloodirect DNA Polymerase Extraction-free DNA amplification from whole blood samples with abm’s Bloodirect DNA Polymerase. With this unique enzyme, DNA can be amplified in reactions containing up to 20% (v/v) whole blood sample without a separate DNA purification step. As a result, the use of abm’s Bloodirect DNA Polymerase offers an overall reduction in contamination risk, experimental run time, and cost of genetic testing. With abm’s Bloodirect DNA Polymerase, you can:  Eliminate lengthy DNA purification steps  Achieve direct amplification from fresh or frozen blood preserved in EDTA, citrate or heparin  Designed for Whole blood PCR

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ABMG462

DNA Polymerase

100 μl (100 U)

$50.00

ABMG463

DNA Polymerase

400 μl (400 U)

$160.00

ABMG465

2x PCR Mastermix

5.0 ml (200 rxns)

$179.00

EvaGreen 2x qPCR MasterMix Products Guaranteed high-performance real-time qPCR using abm’s EvaGreen 2x qPCR MasterMix. abm’s EvaGreen 2x qPCR MasterMix provides all ingredients necessary for quantitative PCR in a premixed and optimized format. Available with the option of ROX or fluorescein as the internal passive reference dye, abm’s EvaGreen 2x qPCR MasterMix offers unparalleled performance in sensitivity, signal-to-noise ratio, and complete elimination of primer dimers. Abm’s EvaGreen 2x qPCR MasterMix is designed for:     

Gene-expression analysis Gene knockdown & Microarray validation High throughput applications Virus detection and quantification Storage at – 20o C, protected from light

With abm’s EvaGreen 2x qPCR MasterMix, you can:    

Enable streamlined protocol in a simple reaction set-up Allow accurate quantification of a variety of gene targets Reduce pipetting steps to minimize the risk of contamination Compatible with most real-time PCR instruments

ABMMastermix-S

MasterMix with No Dye

ABMMastermix-R

MasterMix with ROX

ABMMastermix-LR

MasterMix with Low ROX

ABMMastermix-iC

MasterMix optimized for the iCycler

$165.00 4 x 1.25 ml(20 μl per reaction)

$165.00 $165.00 $165.00

TaqProbe 2x qPCR MasterMix Products Ultimate sensitivity in real-time qPCR with abm’s TaqProbe 2x qPCR MasterMix. abm’s TaqProbe 2x qPCR MasterMix is designed for high throughput quantitative PCR using TaqMan® probe-based chemistry. Available with the option of ROX or fluorescein as the internal passive reference dye, abm’s TaqProbe 2x qPCR MasterMix offers superb performance in sensitivity and signal-to-noise ratio. The multiplex formulation supports quantitative amplification and detection of up to four targets simultaneously with consistent and reliable results. Abm’s TaqProbe 2x qPCR MasterMix is designed for:       

Gene-expression analysis Gene knockdown & Microarray validation SNP genotyping assays CHiP Copy number variation High throughput applications Virus detection and quantification Storage at – 20o C, protected from light

With abm’s TaqProbe 2x qPCR MasterMix, you can:    

Enable streamlined protocol in a simple reaction set-up Allow accurate quantification of a variety of gene targets Reduce pipetting steps to minimize the risk of contamination Compatible with most real-time PCR instruments

ABMMastermix-PS

MasterMix with No Dye

$165.00

ABMMastermix-P

MasterMix with ROX

$165.00

ABMMastermix-PL

MasterMix with Low ROX

ABMMastermix-PC

MasterMix optimized for the iCycler

4 x 1.25 ml(20 μl per reaction)

$165.00 $165.00

ABMMastermix-PM

MasterMix - Multiplex

$165.00

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EvaGreen and TaqProbe qPCR MasterMix Selection Guide Cat. Number

8

Product Name

ABMMastermix-R

EvaGreen 2X qPCR MasterMix-ROX

ABMMastermix-P

TaqProbe 2X qPCR MasterMix-ROX

ABMMastermix-LR

EvaGreen 2X qPCR MasterMix-Low ROX

ABMMastermix-PL

TaqProbe 2X qPCR MasterMix-Low ROX

ABMMastermix-iC

EvaGreen 2X qPCR MasterMix-iCycler

ABMMastermix-PC

TaqProbe 2X qPCR MasterMix-iCycler

ABMMastermix-S

EvaGreen 2X qPCR MasterMix

ABMMastermix-PS

TaqProbe 2X qPCR MasterMix-no Dye

ABMMastermix-PM

TaqProbe 2X qPCR MasterMix-Multiplex

qPCR Instruments •ABI 7000, 7300, 7700, 7900, 7900HT, StepOnePlus™, StepOne™, OpenArray, Prism™ Sequencing Detection Series • Biometra TOptical • Fluuidigm BioMark™ • Wafergene SmartChip System • TianLon TL998 System ®

• ABI® 7500 Viia™, QuantStudio • BioGene InSyte™ • Illumina Eco • Stratagene® Mx3000, Mx3005, Mx4000 • Analytikjena qTower Series

• BioRad® iCycler®, iQ™5, MyiQ™

• BioRad® CFX96, CFX384, Chromo4™, CFX Connect™, Opticon 2, MiniOpticon™ • Roche LightCycler® (2.0, 1.5, 480, 1536, Nano) • MJ Research Opticon™, Opticon™ 2, Chromo® 4 • Eppendorf® Realplex 4 • BioGene SynChron™ • Corbett Rotor-gene® (3000, 6200, 62H0, 6500, 65H0, 6600) • Eppendorf Mastercycler® realplex (s, 4, 4s) Pro (S, 384), Nexus (gradient, eco, flat) • Cepheid SmartCycler®, GeneXpert • Enigma® ML • Idaho LightScanner® (24, 32), RapidCycler®2, R.A.P.I.D (LT, LT Food), RAZOR EX, JBAIDS • Qiagen Rotor-Gene™ (Q, 6000) • Takara Dice™ • Thermo Scientific PikoReal • DNA-Technology DT96, DTlite, DT-322 • Bioer LineGene (3310/3320, K FQD-48A, I, II, 9620, 9640, 9660, 9680) • Bioneer Exicycler™ Any qPCR instrument that supports multiplex reactions

DNA Gel Extraction Kit DNA Gel Extraction Kit provides a simple and efficient method for DNA extraction from agarose gels and reaction mixtures. It is based on the solubilisation of agarose gel and selective adsorption of nucleic acids on specially prepared Genclean Column at high salt concentration. The whole procedure takes 20 min without phenol-chloroform extraction and alcohol precipitation steps. Genclean Column which are bound with DNA are washed to remove contaminants, and the pure DNA is eluted with TE buffer or water. This method is faster and easier to perform than other organic-based extraction methods as it requires fewer manipulations. This kit contains a specific indicator to allow the the pH value to be monitored and to make sure that the agarose gel dissolves thoroughly. GRGK2042

DNA Gel Extraction Kit

100 Preps

$88.46

Total RNA Extraction Kit (Trizol Column) Trizol Reagent can be used for extracting total RNA:  From animal and plant tissues, various microorganisms and cultured cells. Once chloroform has been added and the solution has been centrifuged, this kit will thoroughly lyse the sample and separate the solution into the top or aqueous phase, middle phase and bottom or organic phase. RNA remains exclusively in the aqueous phase. After transferring the aqueous phase to a new tube, the RNA is recovered by precipitation with isopropyl. DNA is recovered by precipitation with ethanol in middle phase. Protein is recovered by precipitation with Isopropyl alcohol in organic phase (bottom layer).  For small samples (50-100 mg organization, 5 × 106 cells) and for large samples (> 1g organization or > 107 cells).This kit can handle a wide range of samples or source material, for example animal tissue, plant matter and bacteria. The highly pure RNA is recovered with extremely little protein and even less genomic DNA, and can be used directly in various molecular biology experiments such as Northern hybridization, dot-blot, hybridization, mRNA purification, in-vitro translation, RNA lysase protection analysis, RT-PCR and cDNA library construction, etc. GRGK3012

Total RNA Extraction Kit (Trizol Column)

50 Preps

$159.23

Trizol Reagent Trizol Reagent can be used for extracting total RNA from animal and plant tissues, various microorganisms and cultured cells. This kit is universal and can recover total RNA from various samples. It is fast and convenient and the whole procedure can be completed in 60 minutes or less. Once chloroform has been added and the solution has been centrifuged, this kit will thoroughly lyse the sample and separate the solution into the top or aqueous phase, middle phase and bottom or organic phase. RNA remains exclusively in the aqueous phase. After transferring the aqueous phase to a new tube, the RNA is recovered by precipitation with isopropyl. The highly pure RNA is recovered with extremely little protein and even less genomic DNA, and can be used directly in various molecular biology experiments such as Northern hybridization, dot-blot, hybridization, mRNA purification, in-vitro translation, RNA lysase protection analysis, RT-PCR and cDNA library construction, etc.  This product is easy to distinguish between aqueous and organic phase after centrifugal stratification, is convenient to operate and is a pale yellow solution. GRGK3004

Trizol Reagent

100 ml

$159.23

PCR Purification Kits PCR Product Purification Kit provides a simple and efficient method for DNA extraction from PCR reaction systems, various enzymatic reactions and ligation systems. This kit utilizes a process based on the selective adsorption of nucleic acids in a spin column, on specially prepared silica-gel based membrane (at high salt concentration), which adsorbs selectively up to 20 μg of DNA fragments in the presence of specialized binding buffers. Nucleotides, enzymes, mineral oil and other impurities do not bind to the membrane. DNA fragments can be eluted with elution buffer. Specific indicator system is included in the whole process to guarantee successful DNA adsorption. Purified DNA can be used in any downstream applications, such as sequencing, labeling, restriction enzymatic digestions, ligations or transformations. Note: Prepare your sample or DNA fragment(s) through the electrophoresis process, followed by a DNA Gel Extraction kit (above).    

Simple & Efficient method to extract DNA from PCR reaction systems, various enzymatic reactions and ligation systems 15 to 20 minute protocol Suitable for purifying DNA fragments as low as 80 bp No phenol / chloroform extraction or ethanol precipitation.

GRGK2051

PCR Purification Kit

50 Preps

$47.77

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Genomic DNA Isolation Kit for Cells & Tissues The Genomic DNA Isolation Kit provides a simple and rapid method for high quality genomic DNA purification from various sources: cell cultures, plant and mammalian tissues, as well as epithelial samples. The kit is based on selective DNA precipitation from crude lysates using detergents. The entire procedure takes only 20-25 minutes without organic solvent or ethanol precipitation. This kit contains a membrane embedded in a spin column capable of binding up to 30 μg of genomic DNA. Nucleotides, proteins, salts and other impurities do not bind to the column. The purified high molecular weight genomic DNA is suitable for direct use in all common molecular biology applications, such as: PCR, restriction digestion, cloning, DNA sequencing and Southern blot analysis. 2-10 μg is the typical yield of highly pure (OD260/OD280 = 1.8 – 2.0) genomic DNA, from a sample of either 1-100 mg of tissue, or 105-107 of cultured cells. GRGK0122

Genomic DNA Isolation Kit for Cells & Tissues

100 Preps

$212.31

Genomic DNA Isolation Kit for Blood This kit is designed for fast isolation of genomic DNA from whole blood (Human or Animal). Up to 1 ml of blood can be processed per purification without the need for ethanol precipitation or toxic reagents. The kit contains a membrane embedded in a spin column for binding up to 30μg of genomic DNA. Nucleotides, proteins, salts, and other impurities do not bind to the Column. The highly pure (OD260/OD280 = 1.8 – 2.0) genomic DNA can be applied in most molecular biology experiments, including restriction digestion, PCR, Restriction digestion, cloning, DNA Sequencing and Southern blot analysis. GRGK1041

Genomic DNA Isolation Kit for Blood

50 Preps

$111.46

Genomic DNA Isolation Kit for Bacterial Cells This mini prep kit is designed for isolation of genomic DNA from most bacteria and/or cultured cells. You can produce genomic DNA in 50 minutes or less without using toxic reagents such as phenol or requiring ethanol precipitation steps. The kit contains a membrane embedded in a spin column for binding up to 30μg of genomic DNA. Nucleotides, proteins, salts, and other impurities do not bind to the Column.The OD260/OD280 can range from 1.7 to 1.9, and its length varies from 50 kb to 150 kb. The purified high molecular weight genomic DNA is suitable for direct use in all common molecular biology applications: PCR, restriction digestion and cloning, DNA Sequencing and Southern blot analysis. GRGK1071

Genomic DNA Isolation Kit for Bacterial Cells

50 Preps

$111.46

Mini Plasmid Purification Kits The Plasmid Miniprep Kit provides a simple, rapid and cost-effective method for plasmid isolation from E.coli cultures; Obtain high yields of  80 % in 20 minutes or less. The kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column; Plasmid DNA is selectively adsorbed in silica gel-based column and other impurities such as proteins, salts and nucleotides do not bind to the column. Plasmid DNA can be eluted in a small volume of Tris buffer and up to 20 μg of high copy plasmid DNA can be recovered without the use of ethanol precipitation or phenol/chloroform extraction. Purified DNA can be used in any downstream application such as sequencing, transformation, restriction enzymatic digestion and ligation.

Midi Plasmid Purification Kits The Plasmid Midiprep Kit provides a simple, rapid and cost-effective method for plasmid isolation from E.coli cultures; Obtain high yields of  80 % in 30 minutes or less The kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column; Plasmid DNA is selectively adsorbed in silica gel-based column and other impurities such as proteins, salts and nucleotides do not bind to the column. Plasmid DNA can be eluted in a small volume of Tris buffer and up to 70 μg of high copy plasmid DNA can be recovered without the use of ethanol precipitation or phenol/chloroform extraction. Purified DNA can be used in any downstream application such as sequencing, transformation, restriction enzymatic digestion and ligation.

Maxi Plasmid Purification Kits The Plasmid Maxiprep Kit provides a simple, rapid and cost-effective method for plasmid isolation from E.coli cultures. Obtain high yields of  80 % in 30 minutes or less. The kit utilizes an exclusive silica-based membrane technology in the form of a convenient spin column; Plasmid DNA is selectively adsorbed in silica gel-based column and other impurities such as proteins, salts and nucleotides do not bind to the column. Plasmid DNA can be eluted in a small volume of Tris buffer and up to 1000 μg of high copy plasmid DNA can be recovered without the use of ethanol precipitation or phenol/chloroform extraction. Purified DNA can be used in any downstream application such as sequencing, transformation, restriction enzymatic digestion and ligation. GRGK2001

Mini Prep Kit (up to 20 μg)

50 Preps

$47.77

GRGK2002

Mini Prep Kit (up to 20 μg)

300 Preps

$221.16

GRGK2005

Midi Prep Kit (up to 70 μg)

50 Preps

$79.62

Maxi Prep Kit (500 – 1000 μg)

10 Preps

$132.69

GRGK2008 10

10

SafeView - Environmentally Friendly Agarose Stains SafeView™ products represent a new and safe class of nucleic acid stains for the visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels; a great alternative to toxic Ethidium Bromide or SYBR® Safe from other competitors. The dyes are developed to replace toxic Ethidium Bromide (EtBr, a potent mutagen), commonly used in gel electrophoresis for visualization of nucleic acids in agarose gels. Safe Green, SafeView™ classic and SafeView Plus are non-carcinogenic as indicated by the Ames-test. The results are negative in both the mouse marrow chromophilous erythrocyte micronucleus and mouse spermary spermatocyte chromosomal aberration tests.

SafeGreen™ Safe-Green™ represents a new and safe nucleic acid stain for the visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose and polyacrylamide gels.  Safe Green works as a dye which is added to your sample directly, allowing your dsDNA, ssDNA and RNA to fluoresce under UV excitation and/or LED light  Eliminates the need to add stain to your gel or buffer  Dispose Safe-Green™ as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange; Propidium iodide).

SafeView™ Classic SafeView™ Classic represents a new and safe class of nucleic acid stains for the visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose and polyacrylamide gels.    

This stain is developed to replace toxic Ethidium Bromide (EtBr, a potent mutagen) SafeView Classic is used in the exact same fashion as EtBr, by adding it directly to your agarose gel When viewed under LED light, dsDNA and ssDNA will emit a green light, while RNA will appear red Dispose SafeView™ Classic as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange; Propidium iodide).

SafeView™ Plus SafeView™ Plus represents a new and enhanced safe class of nucleic acid stain for the visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose and polyacrylamide gels.     

This stain is developed to replace toxic Ethidium Bromide (EtBr, a potent mutagen) SafeView Plus has higher sensitivity and enhanced performance, as compared to SafeView Classic SafeView Plus is used as a post stain which is added to your buffer, eliminating the need to add it to your agarose gel When viewed under LED light, dsDNA and ssDNA will emit a green light, while RNA will appear red Dispose SafeView™ Plus as you would any other non-carcinogenic fluorescent dye (eg. Acridine orange; Propidium iodide).

SafeGreen™

SafeView™

SafeView™ Plus

Staining Colour

Green Only

Green for ssDNA & ds DNA Red for RNA

Green Only

Exitation Wavelength

290 nm

290& 490 nm

490 nm

Emission Wavelength

490 nm

605 nm

520 nm

Sensitivity Range

0.2 – 0.6 ng

0.1 – 0.3 ng

0.05 – 0.1 ng

Suggested Light Source

UV &/or Blue LED

UV &/or Blue LED

UV &/or Blue LED

Loading Recommendations

Add a 1:5 Dye to Sample ratio to the sample only

5 μl to Gel Solution & 5 μl to Buffer

1:5,000 Dye to Buffer ratio to Buffer (TE, TAE or TBE)

Is Staining Solution Reusable?

No

No

Yes, up to 4 times

Packaging

1.0 ml/pk

1.0 ml/pk

1.0 ml/pk

Code

ABMG108-G

ABMG108

ABMG468

Price

$35.00/pk

$35.00/pk

$70.00/pk

11

DNA Ladders & Protein Markers ExcelBand 100 bp DNA Ladder Description: The ExcelBand 100 bp DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct loading on gel. The DNA marker is composed of 11 individual DNA fragments: 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (1.5 kb and 500 bp) for easy referencing. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Source: Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA. Range: 100 ~ 1,500 bp Number of Bands: 11 Concentration: 50 μg/ 500 μl

TECDNA02R

Recommended loading volume: 5 μl/ well

18.95

$

for 500 μl

Storage at: Room temperature

≥ 6 months

4° C

≥ 12 months

-20° C

≥ 36 months

ExcelBand 100 bp + 3K DNA Ladder Description: The ExcelBand 100 bp+3K DNA Ladder is a ready-to-use DNA ladder, r, which is pre-mixed with loading dye for direct loading on gel. The DNA ladder is composed of 12 individual DNA fragments: 3k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200 and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (1.5 kb and 500 bp) for easy referencing. In addition, two tracking dyes, Xylene cyanol FF and Orange G which mimic the migration of 4,000 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Source: Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA. Range: 100 ~ 3,000 bp Number of Bands: 12 Concentration: 54 μg/ 500 μl

TECDNA06R

Recommended loading volume: 5 μl/ well

18.95

$ Storage at: Room temperature

≥ 6 months

4° C

≥ 12 months

-20° C

≥ 36 months

12

for 500 μl

ExcelBand 1 KB (0.25 to 10 kb) DNA Ladder Description: The ExcelBand 1 KB (0.25-10 kb) DNA Ladder is a ready-to-use DNA A ladder, which is pre-mixed with loading dye for direct loading on gel. The DNA ladder is composed of 13 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (3 kb and 1 kb) for easy referencing. In addition, two tracking dyes, Xylene cyanol FF F and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA A during electrophoresis are also added for real time monitoring. Source: Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA. Range: 250 ~ 10,000 bp Number of Bands: 13 Concentration: 50 μg/ 500 μl Recommended loading volume: 5 μl/ well

TECDNA20R Storage at: Room temperature

≥ 6 months

4° C

≥ 12 months

-20° C

≥ 36 months

18.95

$

for 500 μl

ExcelBand 1 KB Plus (0.1 to 10 kb) DNA Ladder Description: The ExcelBand 1 KB Plus (0.1-10 kb) DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct loading on gel. The DNA Ladder is composed of 19 individual DNA fragments: 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 900, 800, 700, 600, 500, 400, 300, 200, and 100 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains three enhanced bands (3 kb, 1.5 kb and 500 bp) for easy referencing. In addition, three tracking dyes, Xylene cyanol FF, Bromophenol blue and Orange G which mimic the migration of 4,000 bp, 500 bp and 50 bp dsDNA during electrophoresis are also added for real time monitoring. Source: Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA. Range: 100 ~ 10,000 bp Number of Bands: 19 Concentration: 86 μg/ 500 μl

TECDNA22R

Recommended loading volume: 5 μl/ well

33.99

$

for 500 μl

Storage at: Room temperature

≥ 6 months

4° C

≥ 12 months

-20° C

≥ 36 months

13

ExcelBand 50 bp DNA Ladder Description: The ExcelBand 50 bp DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct loading on gel. The DNA marker is composed of 17 individual DNA fragments: 1.5k, 1.2k, 1k, 900, 800, 700, 600, 500, 450, 400, 350, 300, 250, 200, 150, 100, and 50 bp derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (500 bp and 200 bp) for easy referencing. In addition, the low range Orange G tracking dye which mimics the migration of a 50 bp dsDNA during electrophoresis is also added for real time monitoring. Source: Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA. Range: 50 ~ 1,500 bp Number of Bands: 17 Concentration: 54 μg/ 500 μl

TECDNA24R

Recommended loading volume: 5 μl/ well

33.99

$

for 500 μl

Storage at: Room temperature

≥ 6 months

4° C

≥ 12 months

-20° C

≥ 36 months

ExcelBand XL 25 kb DNA Ladder - Broad Range (up to 25 kb) Description: The ExcelBand XL 25 kb DNA Ladder is a ready-to-use DNA ladder, which is pre-mixed with loading dye for direct loading on gel. The DNA Ladder is composed of 14 individual DNA fragments: 25k, 10k, 8k, 6k, 5k, 4k, 3k, 2.5k, 2k, 1.5k, 1k, 750, 500, and 250 base pairs derived from a mixture of PCR products and specifically digested plasmid DNA. This product contains two enhanced bands (3 kb and 1 kb) for easy referencing. In addition, two tracking dyes, Xylene cyanol FF and Bromophenol blue which mimic the migration of 4,000 bp and 500 bp dsDNA during electrophoresis are also added for real time monitoring. Source: Phenol extracted PCR products and dsDNA digested with specific restriction enzymes, equilibrated in 10 mM Tris-HCl (pH 8.0) and 10 mM EDTA. Range: 100 ~ 25,000 bp Number of Bands: 14 Concentration: 51.6 μg/ 500 μl

TECDNA26R

Recommended loading volume: 5 μl/ well

33.99

$ Storage at: Room temperature

≥ 6 months

4° C

≥ 12 months

-20° C

≥ 36 months

14

for 500 μl

ExcelBand Pink Blue Protein Marker Description: The ExcelBand Pink Blue Protein Marker is a two-color protein standard with 11 pre-stained proteins covering a wide range of molecular weights from 10 to 175 kDa in Tris-Glycine buffer (10 to 165 kDa in Bis-Tris (MOPS) buffer and 7 to 165 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a pink chromophore except for three reference bands of blue color (at 10, 40, and 90 kDa, respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The ExcelBand Pink Blue Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Contents: Approximately 0.2~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 1 mM DTT, 4.8 M urea, and 12% (v/v) glycerol). Quality Control: Under suggested conditions, the ExcelBand Pink Blue Protein Marker resolves 11 major bands in 15% SDS-PAGE (Tris-Glycine buffer) and after Western blotting to nitrocellulose membrane. Number of Pre-Stained Protein Bands: 11

Storage at: 4° C

≥ 3 months

-20° C

≥ 24 months

SMOBIOPM2400

2x250 μl

$84.99

SMOBIOPM2700

2x250 μl

$99.45

ExcelBand 3-Colour Broad Range Protein Marker Description: The ExcelBand 3-color Broad Range Protein Marker is a ready-touse three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine buffer (3.5 kDa to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa, respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The ExcelBand 3-color Broad Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Contents: Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). Quality Control: Under suggested conditions, the ExcelBand 3-color Broad Range Protein Marker resolves 13 major bands in SDS-PAGE (Bis-Tris gel, MES buffer) and after Western blotting to nitrocellulose membrane.

Number of Pre-Stained Protein Bands: 13

Storage at: 4° C

≥ 3 months

-20° C

≥ 24 months

15

ExcelBand 3-Colour Pre-Stained Protein Ladder Broad Range Description: The 3-color Pre-Stained Protein Ladder Broad Range is a ready-to-use three-color protein standard with 15 pre-stained proteins covering a wide range of molecular weights from 5 to 245 kDa in Tris-Glycine Buffer (3.5 to 235 kDa in Bis-Tris (MOPS) buffer and Bis-Tris (MES) buffer). Proteins are covalently coupled with different chromophores for easy identification of bands, with three reference proteins carrying enhanced intensity corresponding to a blue band at 20 kDa, green at 40 kDa, and red at 75 kDa, respectively, as separated on SDS-PAGE (Tris-Glycine buffer). The 3-color Pre-Stained Protein Ladder Broad Range is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. Contents: Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). Quality Control: Under suggested conditions, the ExcelBand 3-color Protein Ladder Broad Range resolves 15 major bands in SDS-PAGE (Bis-Tris gel, MES buffer) and after Western blotting to nitrocellulose membrane. Storage at: 4° C

≥ 3 months

-20° C

≥ 24 months

Number of Pre-Stained Protein Bands: 15 SMOBIOPM5200

2x250 μl

$99.95

dNTP Set The dNPT set consists of 100mM aqueous solutions of dATP, dCTP, dGTP and dTTP each supplied in separate vials. Since the nucleotides are provided separately, the dNTP Set offers maximum flexibility in preparation of reaction mixes for different applications. Applications include: PCR, long PCR, RT-PCR, cDNA synthesis, primer extension, DNA sequencing, DNA labeling. Form: dATP, dCTP, dGTP and dTTP.

ABMG050

dNTP Set

4 x 0.25 ml (100 mM each)

$75.00

dNTP Mix The dNTP Mix is a ready-to-use aqueous solution containing dATP, dCTP, dGTP and dTTP, each at a final concentration of 10mM. The Mix reduces the number of pippeting steps and the risk of errors. Applications include: Ready to use in PCR, long-PCR, RT-PCR, cDNA synthesis, primer extention and DNA labeling.

ABMG010

dNTP Mix

250l (100 mM each)

$35.00

ABMG128

dNTP Mix

500l (100 mM each)

$45.00

ABMG129

dNTP Mix

1.0 ml (100 mM each)

$65.00

FREE Shipping On Consumable Orders Over $150.00 (Ground Only) ® Diamed Lab Supplies Inc. All rights reserved. Diamed, DiaTec, DiaSoft, DiaStretch, DiaGrip, TecLG, Tec Choice are trademarks or registered trademarks of Diamed Lab Supplies Inc. Terms & Conditions: Prices and promotions may change without notice. Taxes and shipping charges, if applicable, are not shown. Diamed Lab Supplies Inc. is not responsible for pricing or other errors and reserves the right to modify or cancel orders if such errors occur. Promotional & Free Items: Must be requested at time of order to receive. Void where prohibited by institutional policy. Qualifying amount calculated before freight and applicable taxes.

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LS-vJan17-2014