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the amount of input DNA. (D) ChIP-qPCR of SUZ12 of the promoter region of miR-99a/miR-449a after. siRNA treatment targeting si-NC or si-ANRIL in SGC-7901 ...
Long noncoding RNA ANRIL indicates a poor prognosis of gastric cancer and promotes tumor growth by epigenetically silencing of miR-99a/miR-449a Supplementary Material
Figure S1: (A) qRT-PCR was performed to detect p15INK4B and p16INK4A expression after ANRIL knockdown. (B) ChIP-qPCR of H3K27me3 and EZH2 of the promoter region of p15INK4B /p16INK4A locus after siRNA treatment targeting si-NC or si-ANRIL in SGC-7901 cells, Antibody enrichment was quantified relative to the amount of input DNA. Antibody directed against IgG was used as a negative control. (C) ChIP-qPCR of H3K27me3 and EZH2 of the promoter region of HOXA9 after siRNA treatment targeting si-NC or si-ANRIL in SGC-7901 cells. Antibody enrichment was quantified relative to the amount of input DNA. (D) ChIP-qPCR of SUZ12 of the promoter region of miR-99a/miR-449a after siRNA treatment targeting si-NC or si-ANRIL in SGC-7901 cells, Antibody enrichment was quantified relative to the amount of input DNA.
Figure S2: (A) qRT-PCR was performed to detect the expression after SGC-7901 cells transfected with miR-99a/miR-449a mimics/ inhibitors or miR-NC. (B) SGC-7901 cells were transfected with miR99a/miR-449a mimics or miR-NC. Western blot assays of mTOR/CDK6/E2F1 after transfection. (C) qRT-PCR was uesd to detect E2F1 expression after SGC-7901/BGC-823 cells transfected with E2F1 expression plasmid or control vector.
Figure S3”: proposed model which medicated by ANRIL could form positive feedback loop in gastric cancer cell cycle progression and growth control.
