Long-term sub-lethal effects of low concentration commercial herbicide (glyphosate/pelargonic acid) formulation in Bryophyllum pinnatum
Supplementary Information
Lok R. Pokhrel1,2,*, Istvan Karsai2,*
1
Department of Public Health, Temple University, 1301 Cecil B. Moore Avenue, Ritter Annex, Philadelphia, PA 19122, United States
2
Department of Biological Sciences, East Tennessee State University, Johnson City, TN 37614, United States
*
Corresponding authors:
Tel.: 423-439-5601, E-mail address:
[email protected] (I. Karsai) Tel.: 215-204-9110, E-mail address:
[email protected] (L.R. Pokhrel)
Submitted to: Science of the Total Environment Total Pages: 9 (including cover page) Total Tables: 5 Total Figures: 3
Fig. S1. Schematic showing a pair of leaves and the epiphyllous plantlets (F1 generation/somatic embryos) reproduced on the leaf margins in Bryophyllum pinnatum. Position 1 was the position assigned to the first notch on the leaf margin as counted from the leaf apex, and position 15 being the position closer to the leaf base.
Table S1. General Linear Model Repeatability Test for five repeated measurements taken for epiphyllous plantlets biomass across groups in Bryophyllum pinnatum. Group
Control
Plantlets ’ Position on Leaf Margin Left margin Right margin
RU 1%
RU 3%
RU 5%
Leaf type
Maulchly’s test of sphericity (M)
Leaf 1
Chi square 1365.743
Leaf 2 Leaf 1 Leaf 2
Mean Square 1.993 x 10-6
F
p
0.5
2178.981 909.465
0.5
NA
NA
NA
NA
0.234
>0.5
†
>0.5
†
0.124
>0.5
1.795 x 10
-8
