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Supplementary Materials Table S1. Mass spectrometry data showing significantly differentially abundant proteins identified in camel milk using 2D-DIGE [analysis type: matrix-assisted laser desorption/ionization; database: SwissProt; taxonomy: Mammalian]. Spot Number a

Accession Number b

Pi c

MW d

Cov% e

Score f

219 242 247 253 255

Q9TUM0 P31428 Q9TUM0 Q9TUM0 P01009

8.66 5.9 8.66 8.66 5.37

79158 46109 79158 79158 46878

21 31 25 29 36

59 58 62 65 68

256 270 276 280

P02647 P02675 Q8CG71 P02679

5.56 8.54 5.57 5.37

30759 56577 80959 52106

72 55 19 52

238 205 63 130

285 288

P02679 Q32KP7

5.37 10.18

52106 14535

52 38

130 65

309 316 318 340 333 338 339 343

Q9TUM0 Q9TUM0 P79385 Q9TUM0 P02768 Q9TUM0 Q9TUM0 Q9TUM0

8.66 8.66 7.61 8.66 5.92 8.66 8.66 8.66

79158 79158 53619 79158 71317 79158 79158 79158

44 48 28 35 41 46 42 38

116 184 58 114 88 150 173 131

348 353 357 358 359 360 361 368

Q9TUM0 P02679 Q9TUM0 Q9TUM0 Q9TUM0 P15539 Q6IML7 Q9TUM0

8.66 5.37 8.66 8.66 8.66 9.86 8.72 8.66

79158 52106 79158 79158 79158 57449 31329 79158

43 46 30 44 48 18 33 45

175 145 110 143 175 60 64 123

373 384 396 398

Q9TUM0 P02750 Q9GK12 Q9TUM0

8.66 6.45 9.1 8.66

79158 38382 21763 79158

50 42 31 36

171 71 58 128

400

Q7Z713

5.39

17650

52

60

407 409

Q9H3Z7 Q8MJ14

8.9 6.74

53205 22863

16 37

57 59

413 433 438 440 454 459 462 472 474 492

P02790 Q9TUM0 Q9TUM0 Q8R0F8 Q5E9H8 Q9TUM0 P01876 Q96SZ5 Q9TUM0 Q95KI3

6.55 8.66 8.66 7.59 9.3 8.66 6.08 5.68 8.66 8.95

52385 79158 79158 25484 42307 79158 38486 30074 79158 41206

48 42 48 42 25 46 42 24 48 22

96 139 158 58 60 129 58 59 146 58

500 501 516 517 539 543 546 553 561 562

Q9TUM0 P00435 Q6GPH4 Q8NBT0 P02675 Q68G74 Q6AYB4 Q2KJ64 P79385 P79385

8.66 6.74 8.57 7.31 5.37 8.66 5.41 6.09 6.15 6.15

79158 22930 35857 45551 52106 40445 55170 35272 46722 46722

51 40 40 28 50 21 24 34 29 25

172 60 62 60 129 73 59 62 63 58

564 568 572 579 574 578 582 585

P79385 P46065 P06323 P79385 P79385 Q9TUM0 P04217 Q9QYE2

6.15 4.4 9.13 6.15 6.15 8.66 5.58 7.81

46722 23723 14908 46722 46722 79158 54809 76169

27 62 37 34 34 25 37 19

65 64 60 75 79 92 90 57

587 588 594 595 596 539

Q6AYB4 Q28560 Q2KJ64 Q13595 P02675 P70097

5.41 5.62 6.09 11.27 5.37 10.04

55170 58814 35272 32726 52106 18714

23 20 36 39 48 50

59 60 61 58 120 65

543 548 553 561 562

Q68G74 Q6AYB4 Q2KJ64 P79385 P79385

8.66 5.41 6.09 6.15 6.15

40445 55170 35272 46722 46722

21 24 34 29 25

73 55 54 63 58

560 570 576

P79385 P46065 P06323

6.15 4.4 6.15

46722 23723 46722

27 62 34

65 64 75

a

579 578 582 586 590 588 593

P79385 Q9TUM0 P04217 Q9QYE2 Q6AYB4 Q28560 P34896

6.15 8.66 5.58 7.81 5.41 5.62 6.09

46722 79158 54809 76169 55170 58814 35272

34 25 37 19 23 20 36

79 92 90 59 59 60 61

600 596 597 604 615 616 621 629

Q13595 P02675 P70097 Q68SB1 P00738 P00747 P32261 Q62803

11.27 5.37 10.04 9.54 6.13 7.04 6.1 6.09

32726 52106 18714 62870 45861 93247 52484 59059

39 48 50 30 21 30 24 26

58 120 65 57 70 77 60 64

644 662 664 723 769 793 796 803

Q91Z85 P16261 Q8HX86 P10909 Q9NSQ0 O14791 Q3MID3 P15169

6.32 9.97 6.46 5.89 9.73 5.6 8.08 6.86

58312 35375 28752 53031 12568 44009 56863 52538

22 30 35 26 46 32 26 33

59 61 59 78 68 59 85 62

819 827 828 832 847 849 856 917

Q62803 Q9UHR4 Q9CTN5 Q3UR70 P24310 Q8NBP0 Q29563 P15522

6.09 8.82 5.45 6.24 10.12 6.56 7.62 5.36

59059 57189 67339 98382 9169 97607 36534 17281

14 19 18 11 60 19 23 30

57 58 59 64 70 54 62 59

985 1013 1041 1069 1090 1105 1147 1155

P15522 P15522 O14618 P00710 P00710 P00710 Q8NFU3 Q86X95

5.36 5.36 5.32 5 5 5 5.85 5.82

17281 17281 29536 14877 14877 14877 12579 81606

25 30 30 65 53 45 20 26

57 54 60 66 94 64 51 99

1167

A8K979

9.12

78549

22

66

Spot numbers correspond to those included in the 2D-image.

b

Protein accession number for SWISSPROT Database.

c

Theoretical isoelectric point.

d

Theoretical relative mass.

e

f

% of peptide sequence Coverage.

MASCOT score.

Table S2. Experimental design: nine samples of camel milk were run on five analytical 2D-DIGE gels; the samples were labeled randomly with Cy3 and Cy5; a pooled sample was used as an internal standard and was stained with Cy2. GEL

CY3

CY5

CY2

1

CM-RT (1)

CM-63°C (1)

POOL

2

CM-98°C (1)

CM-RT (2)

POOL

3

CM-63°C (2)

CM-98°C (2)

POOL

4

CM-RT (3)

CM-63°C (3)

POOL

5

CM-98°C (3)

-

POOL