Mapping polyclonal antibody responses to bacterial infection ... - Core

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Apr 13, 2016 - Peptide phage display has most often been applied to the epitope ...... DNA sequences in each barcode-binned FASTA file were translated.


received: 09 December 2015 accepted: 21 March 2016 Published: 13 April 2016

Mapping polyclonal antibody responses to bacterial infection using next generation phage display Ibrahim A. Naqid1, Jonathan P. Owen2, Ben C. Maddison2, Anastasios Spiliotopoulos1, Richard D. Emes1,3, Andrew Warry1,3, Monika A. Tchórzewska4,5, Francesca Martelli4, Rebecca J. Gosling4, Robert H. Davies4, Roberto M. La Ragione6 & Kevin C. Gough1 Mapping polyclonal antibody responses to infectious diseases to identify individual epitopes has the potential to underpin the development of novel serological assays and vaccines. Here, phage-peptide library panning coupled with screening using next generation sequencing was used to map antibody responses to bacterial infections. In the first instance, pigs experimentally infected with Salmonella enterica serovar Typhimurium was investigated. IgG samples from twelve infected pigs were probed in parallel and phage binding compared to that with equivalent IgG from pre-infected animals. Seventyseven peptide mimotopes were enriched specifically against sera from multiple infected animals. Twenty-seven of these peptides were tested in ELISA and twenty-two were highly discriminatory for sera taken from pigs post-infection (P