Mastitis in dairy production

Table of contents Preface Henk Hogeveen

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Organizing committee

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Scientific committee

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Keynotes Mastitis research and control: Where do we come from and where are we going? 23 K. Larry Smith Pathogenesis of mastitis and mammary gland immunity: Where we are and where we should go Alfonso Zecconi

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Mastitis management in an economic framework Henk Hogeveen and Olav Østerås

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New antimicrobial agents in mastitis therapy Stefan Soback and Arthur Saran

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The contribution of science to progress in milking technology Graeme Mein

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Mastitis in small ruminants A. Contreras, A. Sánchez, J.C. Corrales, D. Sierra, C. Gonzalo and M.J. Paape

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Animal welfare: Of increasing importance in modern dairy production Charlotte Hallén Sandgren and Torkel Ekman

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Diagnosis of mastitis and indicators of milk quality J. Hamann

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Mastitis control systems: The Norwegian experience Olav Østerås and Liv Sølverød

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Antimicrobial resistance in mastitis organisms as a public health threat Dik Mevius, Otlis Sampimon and Jan Sol

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Environmental control for mastitis prevention, milk quality and food safety Ynte H. Schukken, Linda L. Tikofsky and Ruth N. Zadoks

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Genetic improvement in mastitis control programmes R.F. Veerkamp and Y. de Haas

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Milk production and mastitis control in emerging dairy countries: The experience in Chile Juan Kruze

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Pathogenesis and immunology Pathogenesis of chronic intramammary Escherichia coli infections Belgin Dogan, Suzanne Klaessig, Kenneth Simpson, Steve Oliver, Raul Almeida and Ynte H. Schukken

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Persistence of Streptococcus uberis in bovine mammary epithelial cells R.A. Almeida, T. Batcha and S.P. Oliver

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Association between genotype of Staphylococcus aureus, recovered at the end of lactation, and cure of the organism in the dry period Randy T. Dingwell, Ken E. Leslie and Parviz Sabour Toxin genes of Staphylococcus aureus isolated from bovine intramammary infection of different clinical characteristics and outcome Changes in leucocyte surface receptor expression and functional responses in dairy cows with Staphylococcus aureus infection H. Nagahata, H. Higuchi, M. Nakamura, K. Yayou, K. Kawai and A. Ogata Vitamin E supplementation and udder health: A meta-analysis N. Moyo, M. Nielen, C. Kruitwagen and A.C. Beynen α-Tocopherol concentration and stereoisomer composition in plasma and milk from dairy cows fed natural or synthetic vitamin E around calving Guillermo Meglia, Søren K. Jensen, Charlotte Lauridsen and Karin Persson Waller Comparison of blood and milk non-specific immune parameters in heifers after calving Renata Piccinini, Enrica Binda, Michela Belotti, Giuseppe Casirani and Alfonso Zecconi Influence of resident milk neutrophils chemiluminescence and viability on the severity of bovine coliform mastitis J. Mehrzad, L. Duchateau and C. Burvenich

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Potential association of polymorphisms in the bovine CXCR2 gene with neutrophil survival and killing ability Gina M. Pighetti and Magdalena Rambeaud Differential cell count and interdependence of udder quarters J. Hamann, A Schröder and R. Merle

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Response of rhlf-transgenic dairy cows to expermentally induced escherichia coli mastitis Paula Hyvönen, Leena Suojala, Toomas Orro, and Satu Pyörälä

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Cell function in the bovine mammary gland: Interdependence of healthy and infected udder quarters R. Merle, A. Schröder and J. Hamann

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Delayed neutrophil apoptosis in bovine subclinical mastitis: Possible involvement in persistent accumulation of cells in milk P. Boutet, L. Gillet, R. Closset, A. Vanderplasschen, F. Bureau and P. Lekeux

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Economics of mastitis and mastitis management Use of partial budgeting to determine the economic benefits of antibiotic treatment during lactation of chronic subclinical mastitis caused by Staphylococcus aureus J.M. Swinkels, R.N. Zadoks and H. Hogeveen

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Impact of epidemiological, zootechnical, managerial and price factors on the economic efficacy of subclinical mastitis treatment during lactation Francis Sérieys

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Stochastic economic modeling of the use of penethamate hydriodide (Mamyzin®) in heifers around calving to control peri-parturient mastitis Mark A. Bryan and Gabriele M. Friton

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Importance of uncertainties in the effects of pathogen specific mastitis on profit in dairy herds estimated by stochastic simulation Søren Østergaard, Mizeck G. G. Chagunda, Torben W. Bennedsgaard, Nicolas C. Friggens and Jens Y. Blom Costs and benefits of improved milking practices in smallholder dairy farms in the 10th region of Chile G. van Schaik, L.E. Green, D. Guzmán, H. Esparza and N. Tadich

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Udder health, treatments and pathogens in organic dairy herds in the Netherlands E.A.A. Smolders, J. van der Werf, D. van de Mortel and A. Kijlstra High milk yields and the risk of mastitis in different herd environments Jack J. Windig, Mario P.L. Calus and Roel F. Veerkamp Association between somatic cell count in early lactation and culling of dairy heifers using Cox frailty models Sarne De Vliegher, Herman W. Barkema, Geert Opsomer, Aart de Kruif and Luc Duchateau

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Therapy and immunization Reducing subclinical and clinical mastitis in dairy heifers by precalving infusion of a teat sealant and/or parenteral antibiotic therapy Scott McDougall, Katrina Parker, Chris Compton and Cord Heuer Chemotherapeutic approaches to bovine mastitis caused by Staphylococcus aureus through strain identification using random amplified polymorphic DNA analysis S.R. Sebastian, K. Vijayakumar and P. Ramadass Efficacy of a cloxacillin dry cow product for treatment of heifers on farms with a low and a high prevalence of heifer mastitis in The Netherlands Otlis Sampimon and Jan Sol Efficacy of a J-5 Escherichia coli bacterin in clinical coliform mastitis of dairy cattle Hubert A. Deluyker, Karen Van Uffel, Gregory D. Elfring, Sophie N. Van Oye, Christopher Dutton and Ian Nanhjiani Effect of carprofen following experimentally induced E. coli mastitis in primiparous cows F. Vangroenweghe, P. Boutet, P. Lekeux, R. Bossuyt, P. Rainard, M.J. Paape, L. Duchateau and C. Burvenich

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Iron uptake and growth responses by Escherichia coli cultured with antibodies from cows immunized with high affinity ferric receptors J.S. Hogan, K.L. Smith, J. Lin, K. Takemura and S.L. Wolf

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In vitro susceptibility of biofilm growing Staphylococcus aureus isolates for 10 antibiotics Marielle B. Melchior, Johanna Fink-Gremmels and Wim Gaastra

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Better management of mastitis treatment: Contribution of consensus conference methodology Jacqueline Bastien, Chantal Legrand, Pierre Brouillet, Natalie Durnford and Gérard Bosquet Leukocyte dynamics and cytokine expression in response to mycobacterial water soluble compounds in bovine mastitis Reena Mukherjee Direct and indirect effects of subclinical mastitis treatment in dairy herds John Barlow, Cameron Nightingale, Ruth Zadoks, Lisa White and Ynte Schukken

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Therapeutic treatment with casein hydrolyzate eradicate effectively bacterial infection in treated mammary quarters in cows Nissim Silanikove, Jose Iscovich and Gabriel Leitner

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Antibiotic resistance in Staphylococcus aureus isolated from bovine udders: Development related to introduction of dry cow therapy? Anne Cathrine Whist

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Orbeseal® and Orbenin® EDC in combination for the treatment of intra-mammary infections at drying off and prevention of new infections during the dry period and early lactation in dairy cows Andrew Bradley, Hamish Newton, Hafid Benchaoui, Nicola Tilt, Vic Cracknell and Tim Rowan

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The safety and efficacy of the immunotherapy treatment Y-Complex in a clinical mastitis study under field conditions Y. Pinchasov, J. Spanier, G. Lietner, J. Pitcovsky, E. Morag, D. Raymond and A. Gordon

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Therapeutic effect of systemic or intra-mammary antibiotic treatment of bovine sub-clinical mastitis during lactation Charlotte Hallén Sandgren, Karin Persson Waller and Ulf Emanuelson

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An alternative treatment trial for Staphylococcus aureus mastitis in organically managed dairy cattle L.L. Tikofsky and R.N. Zadoks

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Prepartum intramammary antibiotic therapy: Effects on udder health, milk production, and reproductive performance in dairy heifers A.A. Borm, L.K. Fox, K.E. Leslie, J.S. Hogan, S.M. Andrew, S.P. Oliver, Y.H. Schukken, D.D. Hancock C.T. Gaskins, W.E. Owens and C. Norman

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Milking technology Milking machine test survey Elizabeth A. Berry, J. Eric Hillerton and Mark Scrivens

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Machine milking risk factors for teat end callosity in dairy cows on herd level F. Neijenhuis G.H. Klungel H. Hogeveen and J.P.T.M. Noordhuizen

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Long-term effects of different pulsation characteristics on teat thickness, teat skin moisture and teat skin pH of dairy cows Stefanie Hansen and Jörn Hamann

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Details of some of the forces applied to the cow’s teat during milking D. Boast, M. Bennett, M. Hale, D.M. Turner, J. Allcock and J.E. Hillerton

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Effects of lactic acid teat dip on chapped teat skin E. Izak, J.P. Araya and L.C. Perren

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Technical success and effectiveness of teat cleaning in automatic milking in Finland M. Hovinen, A-M. Aisla and S. Pyörälä

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Diagnosis of mastitis and indicators for milk quality Use and interpretation of bacteriology in the diagnosis of bovine intramammary infection A.J. Bradley, H. Newton and M.J. Green

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Evaluation of the Petrifilm™ culture system for the identification of mastitis bacteria as compared to standard bacteriological methods Ken Leslie, Matt Walker, Erin Vernooy, Anna Bashiri and Randy Dingwell

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Testing for Staphylococcus aureus in herds with a low bulk milk somatic cell count T.J.G.M Lam and Y.H. Schukken

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Simultaneous detection of mastitis pathogens in milk by multiplex real-time polymerase chain reaction B.E. Gillespie and S.P. Oliver

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Optimised sample sizes for analysing the genetic heterogeneity of mammary pathogen isolates from environmental samples D. Döpfer, R.N. Zadoks, W. Buist and B. Engel

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Mastitis in dairy production

Somatic cell count patterns to improve udder health by genetics and management Yvette de Haas, Herman W. Barkema, Ynte-Hein Schukken, Gerben de Jong and Roel F. Veerkamp Changes in lactate dehydrogenase, N-acetyl-β-D-glucosaminase, and somatic cell count in relation to development of mastitis in dairy cows M.G.G. Chagunda, T. Larsen, M. Bjerring and K.L. Ingvartsen Prevalence of contagious mastitis pathogens in bulk tank milk from Prince Edward Island dairy farms Richard G.M. Olde Riekerink, Herman W. Barkema, Stefan Veenstra, Henrik Stryhn, Doris E. Poole, Randy T. Dingwell and Greg P. Keefe

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A Bayesian approach to interpreting Staphylococcus aureus diagnostic indicators for on-farm decision making Sandra Costello, George Shook and Terry Smith

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Variation in somatic cell count and milk components in fraction collected quarter milk samples Liv Sølverød, Sigrid Simonsen, Andres Waldmann, Olav Østerås, Erik Ropstad

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Using milk L (+) lactate as a diagnostic tool to detect bovine mastitis in the early lactation N.Th. Grabowski, R. Redetzky, A. Sulzer, J. Hamann and G. Klein

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Milk amyloid A (MAA) concentration and somatic cell count (SCC) in the diagnosis of bovine mastitis H.H. Petersen, I.A. Gardner, P. Rossitto, H.D. Larsen and P.M.H. Heegaard

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Diagnosis of mastitis by benzoic acid (benzene sulfonic acid)and sodium carbonate on pregnant and lactating mastitic animals M. Mazhar Ayaz and Masood Akhtar

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Use of on-farm testing of somatic cell count for selection of udder quarters for bacteriological culturing Tine van Werven, Cindy Nijhof, Twan van Bussel and Henk Hogeveen

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Diagnostic value of the California Mastitis Test in comparison to electronically-counted somatic cells in bovine milk R. Redetzky, J. Hamann, N.Th. Grabowski and G. Klein

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Mastitis in small ruminants Aetiological, clinical and epidemiological characterization of clinical mastitis in dairy sheep Dominique Bergonier, Gilles Lagriffoul, Francis Barillet, Rachel Rupp, Aurèle Valognes, Roland Brugidoux, Régis Duquesnel and Xavier Berthelot

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Detection of enterotoxins and virulence genes in Staphylococcus aureus strains isolated from sheep with subclinical mastitis E. De Santis, A. Mureddu, R. Mazzette, C. Scarano and M. Bes

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Subclinical mastitis affects the plasmin system, milk composition and curd yield in sheep and goats: comparative aspects Nissim Silanikove, Fira Shapiro, Gabriel Leitner and Uzi Merin

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Host response reactions of the lactating ovine udder during experimental challenge with Staphylococcus epidermidis Petra Winter, Klemens Fuchs, Franz Schilcher and Ian G. Colditz

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Efficacy of antibiotic treatment at drying-off in curing existing infections and preventing new infections in dairy goats Scott McDougall and Fiona Anniss

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Animal welfare issues related to mastitis Associations between hygiene scores and udder health parameters in organic dairy herds Ilka C. Klaas, Torben W. Bennedsgaard and Mette Vaarst

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Teat lesions and teat necrosis in heifers in The Netherlands Jan Sol, Gerard Wellenberg, Otlis Sampimon, Joost Snoep, Hans Miltenburg and Petra Kock

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Effect of stockperson-dairy cow interaction to mammary gland health Cláudia R. Valle, Andréa R. Ribeiro, Flávio A. Braga, Matheus J.R. Paranhos da Costa, Marcelo S. Rosa and André S.C. Dias

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Control programmes The systems approach to udder health control J.P.T.M. Noordhuizen and H. Hogeveen

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Mastitis in dairy production

The importance of a low SCC premium policy in the reduction of population wide SCC levels Cameron Nightingale and Ynte Schukken Use of SCC in genetic selection for reduced incidence of mastitis: A mixture model approach J. Ødegård, P. Madsen, D. Gianola, G. Klemetsdal, J. Jensen, B. Heringstad and I.R. Korsgaard Genetic improvement of mastitis resistance in Norwegian Red (NRF) Bjørg Heringstad, Yu-Mei Chang, Daniel Gianola and Gunnar Klemetsdal

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Social factors related to mastitis control practices: The role of dairy farmers’ knowledge, attitude, values, behaviour and networks D. Kuiper, J. Jansen, R.J. Renes, C. Leeuwis and H. van der Zwaag

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Mastitis and farmers perceptions and actions: An anthropological perspective to the phenomenon of mastitis M. Vaarst

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Implementation of recommended mastitis prevention management practices and the herd level prevalence of contagious mastitis pathogens on Canadian dairy farms Richard G.M. Olde Riekerink, Herman W. Barkema, Daniel T. Scholl, David F. Kelton and Greg P. Keefe

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A national research network to promote mastitis control in Canada D. Scholl, G. Tomita, S. Messier, H. Barkema, P. Lacasse, X. Zhao and É. Bouchard

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Mastitis control program in The Netherlands: Goal, tools and conditions H.G. van der Zwaag, G. van Schaik and T.J.G.M. Lam

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“FRISKKO - JUVER” (“Healthycow - Udder”) A systematic veterinary approach to udder disease in dairy herds Torkel Ekman, Håkan Landin, Johan Waldner, Mats Gyllenswärd and Charlotte Hallén Sandgren

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Monitoring daily measurements of somatic cell count: Automatic and on-line detection of mastitis I.R. Korsgaard, P. Løvendahl and K.H. Sloth

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A biological model for detecting individual cow mastitis risk based on lactate dehydrogenase Mizeck G.G. Chagunda, Nicolas C. Friggens and Morten D. Rasmussen

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Risk of clinical mastitis within lactation in Dutch dairy cattle Gustavo Monti and Gerben De Jong

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Environmental control Feeding factors associated with clinical mastitis of first parity cows Ann-Kristin Arvidson, Torkel Ekman, Ulf Emanuelson, Anders H. Gustavsson, Charlotte Hallén Sandgren, Kjell Holtenius, Karin Persson Waller and Catarina Svensson

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Isolation of Streptococcus uberis from different sites of the dairy cow S.J. Lacy-Hulbert, R.T. Cursons, M.G. Lopez-Benavides, J.H. Williamson, E.L. Summers, S.M. Pryor and M.W. Woolford

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Risk factors contributing to udder health depression during alpine summer pasturing in Swiss dairy herds M. Walkenhorst, J. Spranger, P. Klocke and W. Schären Streptococcus uberis population dynamics in the New Zealand pastoral dairy farm M.G. Lopez-Benavides, J.H. Williamson, R.T. Cursons, S.J. Lacy-Hulbert and M.W. Woolford A methodology to quantitatively identify effective measures to control environmental mastitis Marc Vissers, Meike te Giffel, Frank Driehuis, Peter de Jong and Jos Lankveld

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Food safety issues related to mastitis On-Farm sources of foodborne pathogens: Isolation from the dairy farm environment S.P. Oliver, S.E. Murinda, L.T. Nguyen, H.M. Nam, R.A. Almeida and S.J. Headrick Shiga Toxin Genes from Escherichia coli strains isolated from mastitic milk Ray T. Cursons, J. Williamson and A. Bean

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Influences on excretion of antibiotic residues in milk with special emphasis on milking frequency 677 Karin Knappstein, Gertraud Suhren, Hans-Georg Walte Antimicrobial susceptibility of Staphylococcus spp. isolated at dry-off and calving P.J. Rajala-Schultz, A. Torres, S. Rajeev, F.J. DeGraves and F. Silveira

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Prepartum intramammary antibiotic therapy: Effect on risk of antibiotic residues in milk from dairy heifers K.J. Steinman, S.M. Andrew, A.A. Borm, L.K. Fox, K.E. Leslie, J.S. Hogan, S.P. Oliver, Y.H. Schukken, W.E. Owens and C. Norman

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Mastitis control in emerging dairy countries Role of selective dry cow therapy in prevention of mastitis in dairy herds with high disease prevalence B.K. Bansal, P.S. Dhaliwal, N.S. Bajwa and S.S. Randhawa Mastitis control in Uruguay: Strengths and weaknesses M. Bouman, R. Bianco, E. Gianneechini, D. Hirigoyen and E. De Torres

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The impact of intervention on mastitis in an emerging dairy developing country-Uganda D.K. Byarugaba, J.L. Nakavuma, C. Laker and M. Vaarst

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Environmental factors affecting somatic cells counts in milk from small dairies in Southern Brazil G. Noro, J.W. Dürr, F.H.D. González and R. Campos

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Risk indicators associated with Staphylococcus aureus subclinical mastitis in smallholder dairy cows in Tanzania F.M. Kivaria, J.P.T.M. Noordhuizen and A.M. Kapaga

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Subclinical mastitis in buffaloes in different herd sizes and milking management systems Chirathalattu Santosh Thomas, Helena Nimmervoll, Caroline Boije, Kerstin Svennersten-Sjaunja, Nils Lundeheim and Karin Östensson

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