Membrane topology of conserved components of the

0 downloads 0 Views 105KB Size Report
Lord [Yersinia pestis]. Pcrd [Pseudomonas aeruginosa]. EscV [Vibrio harveyi]. Bcrd [Bordetella parapertussis]. Escv [Verrucomicrobium spinosum]. LcrD [Stappia ...
Membrane topology of conserved components of the type III secretion system from the plant pathogen Xanthomonas campestris pv. vesicatoria By: Carolin Berger, Guillaume P. Robin, Ulla Bonas and Ralf Koebnik Supplementary Fig. S3

HrcV SctV AscV LcrD PcrD EscV BcrD EscV LcrD EsaV SsaV HrcV HrcV HrcV HrcV

[Xanthomonas vesicatoria] [Photorhabdus luminescens] [Aeromonas salmonicida] [Yersinia pestis] [Pseudomonas aeruginosa] [Vibrio harveyi] [Bordetella parapertussis] [Verrucomicrobium spinosum] [Stappia aggregata] [Edwardsiella tarda] [Shewanella baltica] [Xanthomonas vesicatoria] [Ralstonia solanacearum] [Burkholderia cenocepacia] [Acidovorax avenae]

---------------------MRATRYFAYSGE VAI AALVVAVIGLMIL P ----------------MN-RGFELLRLIGERKDIMLAILLLAVVFMMVLP MKPVLPINNTSNWKSRMNQRTLELLRRIGERKDIMLAILLLAIVFMMVLP ----------------MNPHDLEWLNRIGERKDIMLAVLLLAVVFMMVLP ----------------MN-DLSGLLGRVGERKDILLVVLLLAVVFMMVLP ----------------MN-KLIDILNKVGQRKDIMLAVMLLAIVFMMILP -----------MTSKKSILRLQRAVALATSRNDIVLAVLIVAIVFMMILP -------------MAGKMLQLQGAILRGSRYYDVLLAVVVVAILSLMILP -------------------------MILSQRSDIVLAFLLLSVVFMMILP -----------------MKTVNSWLHKLTARQDIILAAMLLLAVFMMIIP -----------------MNLISRWLSKAAGRHDIILSAMLMVAVFMMILP ---------------------MRATRYFAYSGEVAIAALVVAVIGLMILP --------------------MAKKNAIQDFSGEIGIAALVVAVVALMVLP ---------------------MFKNLKLPAGGEVGIAALVTAIVSLMILP ---------------------MAKRFDSTLVNDLTMAGFLVGVIALMILP :: : .: : :*::*

HrcV SctV AscV LcrD PcrD EscV BcrD EscV LcrD EsaV SsaV HrcV HrcV HrcV HrcV

[Xanthomonas vesicatoria] [Photorhabdus luminescens] [Aeromonas salmonicida] [Yersinia pestis] [Pseudomonas aeruginosa] [Vibrio harveyi] [Bordetella parapertussis] [Verrucomicrobium spinosum] [Stappia aggregata] [Edwardsiella tarda] [Shewanella baltica] [Xanthomonas vesicatoria] [Ralstonia solanacearum] [Burkholderia cenocepacia] [Acidovorax avenae]

LPTPMIDTL L GINITLSVVLLMVTMYVPDSISLSSF PSLL LFTTLLRLSL 79 LPPILLDILIAINMTISVVLMMMAVYINSPLQFSAFPAVLLVTTLFRLAL 83 LPPVALDILIAINMTISVVLLMMAVYINSPLQFSAFPAVLLITTLFRLAL 100 LPPLVLDILIAVNMTISVVLLMIAIYINSPLQFSAFPAVLLVTTLFRLAL 84 LPPLLLDILIAVNITISVVLLMMSVYIGSPLQFSVFPAVLLITTLFRLAL 83 LPTALVDVLIGTNMSIAVVLLMLAIYITKPLEFSAFPAVLLITTLFRLSL 83 LPTTLVDVLIGANMTLSAVLLMVAMYLPSPLAFSSFPSVLLVTTLFRLGI 89 VPPAVLDLLLAINLAISMLLLMLSMYVPGILEFSTFPSLLLITTLFRLSL 87 LPTLMVDSLIGVNLCISAILLMVAVYISDVTQFSAFPSILLLTTLFRLAL 75 LPTPLIDILIAINLTLSITLLMMAVYIREPMEFSAFPSVLLITTLYRLAL 83 LPTPLVDVLIALNLGLSIVLLMIAIYIRDPLEFSVFPSLLLITTLYRLAL 83 LPTPMIDTLLGINITLSVVLLMVTMYVPDSISLSSFPSLLLFTTLLRLSL 79 LPTMLIDALLGLNITLSVVLLMVTMYIPSATSLSAFPSLLLFTTLLRLSL 80 LPPVVIDMLLSVNICISVVLLMVTLYVPSITQLSAFPSVLLFTTLYRLSL 79 LPTLLIDSLLAINLSISVLLLMTTLFIPDAVSLSTFPSLLLFTTLFRLSL 79 :*. :* *:. *: :: *:* :::: :* **::**.*** **.:

HrcV SctV AscV LcrD PcrD EscV BcrD EscV LcrD EsaV SsaV HrcV HrcV HrcV HrcV

[Xanthomonas vesicatoria] [Photorhabdus luminescens] [Aeromonas salmonicida] [Yersinia pestis] [Pseudomonas aeruginosa] [Vibrio harveyi] [Bordetella parapertussis] [Verrucomicrobium spinosum] [Stappia aggregata] [Edwardsiella tarda] [Shewanella baltica] [Xanthomonas vesicatoria] [Ralstonia solanacearum] [Burkholderia cenocepacia] [Acidovorax avenae]

NI ASTKSILLHAEAGHIIESFGELVVGGNL VVGLVVFLIITTVQFI VI AK SVSTTRMILLQADAGKIVYTFGNFVVGGNLIVGIVIFLIITIVQFIVITK SVSTTRMILLQADAGQIVYTFGNFVVGGNLVVGIVIFLIITIVQFLVITK SVSTTRMILLQADAGQIVYTFGNFVVGGNLIVGIVIFLIITIVQFLVITK SVSTTRMILLQADAGQIVNTFGSFVVGGNLVVGIIIFLIITIVQFLVITK SITTTRLILLQGDAGQIVYTFGNFVVGGNLVVGIVVFLIITIVQFMVITK SIATTRLILLQGDAGHIIETFGNFVVGGNLIVGLVVFLILTIVQFVVITK NITTTRLILLHAHAGEIVYTFGNFVVGGNFVVGVVIFLIITLIQFIVITK SITTTRLILLQADAGQIVETFGNFVVSGNLIVGIVIFLIVTIVNFLVITK TISTSRLILLQHDAGEIVYTFGNFAVGGNLAVGLIVFAIITVVQFIVITK TISTSRLILLQHDAGDIVYTFGNFVVGGNLAVGIIIFAIITIVQFIVITK NIASTKSILLHAEAGHIIESFGELVVGGNLVVGLVVFLIITTVQFIVIAK NIASTKSILLHADAGHIIESFGKLVVGGNLVVGLVVFLIITTVQFIVIAK NIASTKSILLHADAGHIIESFGELVVGGNLVVGLVVFLIITTVQFIVIAK NIASTKAILLHADAGHLIESFGQLVVGGNLVVGIVVFLVITIVQFIVIAK .::::: ***: .**.:: :**.:.*.**: **:::* ::* ::*:**:*

129 133 150 134 133 133 139 137 125 133 133 129 130 129 129

HrcV SctV AscV LcrD PcrD EscV BcrD EscV LcrD EsaV SsaV HrcV HrcV HrcV HrcV

[Xanthomonas vesicatoria] [Photorhabdus luminescens] [Aeromonas salmonicida] [Yersinia pestis] [Pseudomonas aeruginosa] [Vibrio harveyi] [Bordetella parapertussis] [Verrucomicrobium spinosum] [Stappia aggregata] [Edwardsiella tarda] [Shewanella baltica] [Xanthomonas vesicatoria] [Ralstonia solanacearum] [Burkholderia cenocepacia] [Acidovorax avenae]

GSERVAEVGARFTLDAMPGKQMSIDADLRGGNLTADEARRKRARLAMESQ GSERVAEVSARFSLDAMPGKQMSIDGDMRAGVIDVNEARERRSIIEKESQ GSERVAEVSARFSLDAMPGKQMSIDGDMRAGVIDVHEARDRRGVIEKESQ GSERVAEVSARFSLDAMPGKQMSIDGDMRAGVIDVNEARERRATIEKESQ GAERVAEVSARFSLDAMPGKQMSIDGDMRAGVIDVNEARARRAVIEKESQ GSERVAEVSARFSLDAMPGKQMSIDGDMRAGVIDVHEARHRRSLIEKESQ GAERVAEVAARFSLDAMPGKQMSIDADLRAGTIDMDEARRRRRTVEKESQ GAERVAEVGARFTLDAMPGKQMAIDADLRAGAITQEEATARRHKVEKENQ GSERVAEVSARFSLDALPGKQMSIDSDIRSGLIDMEEAKRRRTKLGKESQ GSERVAEVSARFSLDAMPGKQMSIDGDMRAGIIDATEARRQRALVQKESQ GSERVAEVSARFSLDGMPGKQMSIDGDMRAGIIDAKEAKRQRAQVQKESQ GSERVAEVGARFTLDAMPGKQMSIDADLRGGNLTADEARRKRARLAMESQ GSERVAEVGARFTLDAMPGKQMSIDADLRAGHLSPEEARKRRALLAMESQ GSERVAEVGARFTLDALPGKQMSIDADLRANLLTPDEARRKRARLAVESQ GSERVAEVGARFTLDALPGKQMSIDADLRAGLLTADEAKAKRARLSMESM *:******.***:**.:*****:**.*:*.. : ** :* : *.

179 183 200 184 183 183 189 187 175 183 183 179 180 179 179

Page 2 of 5

29 33 50 34 33 33 39 37 25 33 33 29 30 29 29

HrcV SctV AscV LcrD PcrD EscV BcrD EscV LcrD EsaV SsaV HrcV HrcV HrcV HrcV

[Xanthomonas vesicatoria] [Photorhabdus luminescens] [Aeromonas salmonicida] [Yersinia pestis] [Pseudomonas aeruginosa] [Vibrio harveyi] [Bordetella parapertussis] [Verrucomicrobium spinosum] [Stappia aggregata] [Edwardsiella tarda] [Shewanella baltica] [Xanthomonas vesicatoria] [Ralstonia solanacearum] [Burkholderia cenocepacia] [Acidovorax avenae]

LHGGMDGAMKFVKGDAIAGLVITMVNILAGIVVGVT YHGMTAGDAANRFA 229 MFGSMDGAMKFVKGDAIASLLIIFVNILGGITIGVTQKGMSASDALQLYA 233 MFGSMDGAMKFVKGDAIAGLIIIFVNILGGVTIGVTQKGLSAADALQLYS 250 MFGSMDGAMKFVKGDAIAGLIIIFVNILGGVTIGVTQKGLAAAEALQLYS 234 MFGSMDGAMKFVKGDAIAGLIIIVVNILGGIAIGVTQKGLSTADALQLYA 233 MYGSMDGAMKFVKGDSIAGLIIIVVNILGGVTIGVTQKGMSASEALELFA 233 LYGAMDGAMKFVKGDAIAGLIIVAVNLLGGMLVGVLQRGLSAGEAVQTYA 239 LHGAMDGAMKFVKGDAIAGLIITAINITAGICIGVMQKGWDIGKAVTTYS 237 LYGAMDGAMKFVKGDAIAGLIIIAVNIIGGILIGTNQRGMSTSEALDIYA 225 LYGAMDGAMKFVKGDAIASIIVILVNIVGGICVGVFMHEMSAGDAAATYA 233 LYGAMDGAMKFVKGDAIASIIVILTNIIGGIAVGVMQHNMSAAEALNTYA 233 LHGGMDGAMKFVKGDAIAGLVITMVNILAGIVVGVTYHGMTAGDAANRFA 229 LHGGMDGAMKFVKGDAIAGLVITLVNILAGIVIGITYHNMTAGEAANRFA 230 LYGGMDGAMKFVKGDAIAGLIITLVNMTAGIAVGVAYHDMTAGDAANRFS 229 LHGGMDGAMKFVKGDAIAGLIITLVNILAGIVVGIMYHSMTAGEAANRFA 229 :.*.***********:**.::: *: .*: :* : ..* ::

HrcV SctV AscV LcrD PcrD EscV BcrD EscV LcrD EsaV SsaV HrcV HrcV HrcV HrcV

[Xanthomonas vesicatoria] [Photorhabdus luminescens] [Aeromonas salmonicida] [Yersinia pestis] [Pseudomonas aeruginosa] [Vibrio harveyi] [Bordetella parapertussis] [Verrucomicrobium spinosum] [Stappia aggregata] [Edwardsiella tarda] [Shewanella baltica] [Xanthomonas vesicatoria] [Ralstonia solanacearum] [Burkholderia cenocepacia] [Acidovorax avenae]

ILSVGDAMVSQIASLLISVAAGVMITRVANENETR-LSSLGLDIGRQLTS ILTVGDGMVSQVPALLIAITAGIIVTRVS---SED-ASDLGSEIGDQVVA ILTVGDGMVSQVPALLIAITAGIIVTRVS---SEE-SSDLGTDIGAQVVA ILTVGDGMVSQVPALLIAITAGIIVTRVS---SED-SSDLGSDIGKQVVA VLTVGDGMVSQVPALLIAITAGIIVTRVS---SDE-SADLGSDIGEQVVA ILTVGDGLVSQIPALFIAITAGIIVTRVS---NED-SSDLGSDIGGQVTA ILTIGDGLIAQIPALFIAICAGIIVTRVQ---TGDGPSNVGTDIGAQVLA ILTIGDGLVSQIPALLISITAGMIVTRVS---ASEDGGALGGDVGNQLLS LLTIGDGLVAQIPALFISITAGFIVTRVS---SEE-NTDLGSDITYQLVK ILSVGDGLIAQIPALLISIAAGIIVTRVP---GEN-RRNLASELTEQILR VLSVGDGLIAQIPSLLISVTAGLIVTRVP---GEQ-RQNLANELVMQVGR ILSVGDAMVSQIASLLISVAAGVMITRVANENETR-LSSLGLDIGRQLTS VLSIGDAMVSQIPSLLISVAAGVMITRVSDEEQAHKQSSLGMEIVRQLST ILSIGDAMVSQIPSLLIAVSAGVMITRVSDESAKQ-GSSLGEDMLRQLGG VLSIGDAMVSQIPALFICMAAGILTTRVADEHRKK-PTSLGEDMTEQLTR :*::**.:::*:.:*:*.: **.: *** :. :: *:

278 279 296 280 279 279 286 284 271 279 279 278 280 278 278

HrcV SctV AscV LcrD PcrD EscV BcrD EscV LcrD EsaV SsaV HrcV HrcV HrcV HrcV

[Xanthomonas vesicatoria] [Photorhabdus luminescens] [Aeromonas salmonicida] [Yersinia pestis] [Pseudomonas aeruginosa] [Vibrio harveyi] [Bordetella parapertussis] [Verrucomicrobium spinosum] [Stappia aggregata] [Edwardsiella tarda] [Shewanella baltica] [Xanthomonas vesicatoria] [Ralstonia solanacearum] [Burkholderia cenocepacia] [Acidovorax avenae]

NARALMAASVLLACFAFVPGFPAVLFLLLAAAVGAGGYTI WRKQR-DISG QPKALLIGGVLLVVFGLIPGFPTLTFLILALVVAGGGYLLFQ-RQRQANA QPKALLIGGLLLVLFGLIPGFPMITFFALSAIVTAGGYFIGL-RQRKAQS QPKAMLIGGVLLLLFGLIPGFPTVTFLILALLVGCGGYMLSR-KQSRNDE QPKALLIGGLLLVLFGLIPGFPTLTFLALALLVGGGGYFMLW-RQRAQAS QPRALLVGGVLLILFALIPGFPKVTFLILAAIVGGGGFFLFY-QQKKQSE QPRALVIAGAISAGLGLIPGMPTLVFFALAAVVGTIGFVLLR-ASQRPPE QPKALMIAAGFMVGFALIPGFPKIPFLILAVVSGVGGFALSR-KQPAESV EPKALLIASGILLGFAAIPGFPPSVFLSLSFLCGAGGIIIIRNRKNVQKS QRQALWIVAAVLALFAALPGFPTLIFIGLGAGLAAIAYSLRRKERQRSPL QPASLQMAGIVMFIFALIPGFPILVFGTLGSAVLGASWWLTRQQKRASAQ NARALMAASVLLACFAFVPGFPAVLFLLLAAAVGAGGYTIWRKQR-DISG SARAMFTASALLMGFALVPGFPSFLFVALATLIFVFGYTLRNRAK-EGDG NARAMLFAAVLLLGFAMVPGFPWELFLLLSALLGFAGYRLARRGDGTSSA NTRSMYLAGALTLGFAAIPGFPAVPFCLLAAGLVGGGQWLSRSRKSATPG . :: . . :. :**:* * *. . :

327 328 345 329 328 328 335 333 321 329 329 327 329 328 328

HrcV SctV AscV LcrD PcrD EscV BcrD EscV LcrD EsaV SsaV HrcV HrcV HrcV HrcV

[Xanthomonas vesicatoria] [Photorhabdus luminescens] [Aeromonas salmonicida] [Yersinia pestis] [Pseudomonas aeruginosa] [Vibrio harveyi] [Bordetella parapertussis] [Verrucomicrobium spinosum] [Stappia aggregata] [Edwardsiella tarda] [Shewanella baltica] [Xanthomonas vesicatoria] [Ralstonia solanacearum] [Burkholderia cenocepacia] [Acidovorax avenae]

TD---QRKLPSASRKGAKGEAPHIRKN------------APDFASPLSMR 362 SQQADLPSLLAQGAGAPAAKSKAKSGS-KAKAGKLSEKEEFAMTVPLLID 377 SNSQDLPAVLAQGAGAPAARSKPKPGS-KPR-GKLGEKEEFAMTVPLLID 393 AN-QDLQSILTSGSGAPAARTKAKTSG-ANK-GRLGEQEAFAMTVPLLID 376 AGSRDLPALLAQGAGAPSAKARGKAGGGKPKAGRLAEQEEFALTVPLLID 378 YDSSDLPSFVAQGAGSPAAKPNKPTPSRNSK-GKLGEQEEFAMTVPLLID 377 GAGPALAGMAADG----QPRTRAPADG----------QAEFAPTVPLIID 371 LATQDKRDLPAMAPTGSKPQPKKKDAN-----------DEFSITVPLLMD 372 RTLNDLPSFAPAVFGDGNVPSFPEGDD---APGGAAKAVTFQLTIPLMVD 368 AAGHSASDHDESGQEGAP-LLIG--------------------ATPLALR 358 GDESHFTEAGKSEMTNVDNMAPG--------------------AVPLILY 359 TD---QRKLPSASRKGAKGEAPHIRKN------------APDFASPLSMR 362 DE---GDALPALLREGSKGKAPTIAEQ------------APSFTVPVGVR 364 AP---GKTVAALQRAGSKGDAPTILRH------------APQFTSALGVR 363 SAKTHSKPIASLLREGGKGEGTAIQHR------------APEFAKPLSIR 366 : .: :

Page 3 of 5

HrcV SctV AscV LcrD PcrD EscV BcrD EscV LcrD EsaV SsaV HrcV HrcV HrcV HrcV

[Xanthomonas vesicatoria] [Photorhabdus luminescens] [Aeromonas salmonicida] [Yersinia pestis] [Pseudomonas aeruginosa] [Vibrio harveyi] [Bordetella parapertussis] [Verrucomicrobium spinosum] [Stappia aggregata] [Edwardsiella tarda] [Shewanella baltica] [Xanthomonas vesicatoria] [Ralstonia solanacearum] [Burkholderia cenocepacia] [Acidovorax avenae]

LSPQLAALLDPARLDQAIESERRQLVELLGLPFPGIAIWQTESLQGMQYE 412 VDAGLQAELEAISLNDELIRVRRALYLDLGVPFPGIHLRFNEGMKEGEYL 427 VDAALQAELEAIALNDELVRVRRALYLDLGVPFPGIHLRFNEGMGPGEYL 443 VDSSQQEALEAIALNDELVRVRRALYLDLGVPFPGIHLRFNEGMGEGEYI 426 VDASLQERLEAMSLNEELVRVRRALYLDFGVPFPGIHLRFNEAMGDGEYL 428 LDSRLQESLEAVALNDELARVRRALYLDLGVPFPGIHLRFNEGMSNGEYL 427 VAARLQPRFEPATLTDDLLQIRRALYFDLGVPFPGIQLRFTEALAANTYT 421 VSAASQNAIDAASMNEELIRVRQALYHDLGVPFPGIHLRFNESLPAGAYK 422 ISSAVRGAIRPEVLDQEVARVRKALYFDLGVPFPGINLRLNDNLREGEYR 418 LPDALARDP---TLAQALATLRAEKLELAGLPLPEILIQPDSGLGPDQFT 405 LGADVPIN----GVKEALQKLRWQWFEQLGVPLPEIQLQIAKKGTLGRCD 405 LSPQLAALLDPARLDQAIESERRQLVELLGLPFPGIAIWQTESLQGMQYE 412 LGAELAKGLDVPALDTAFQQGRHALAEALGLPFPGIAIWKADALQPDSYE 414 LAPDLADRLSATALNDAFETQRMLLQEQIGLPFPGITMWTASDLAAGTFE 413 LSDPLARLLNAERLDQALNKEREGLQTRLGLPFPGAAMWVWDGLQDLRFD 416 : : . * *:*:* : .

HrcV SctV AscV LcrD PcrD EscV BcrD EscV LcrD EsaV SsaV HrcV HrcV HrcV HrcV

[Xanthomonas vesicatoria] [Photorhabdus luminescens] [Aeromonas salmonicida] [Yersinia pestis] [Pseudomonas aeruginosa] [Vibrio harveyi] [Bordetella parapertussis] [Verrucomicrobium spinosum] [Stappia aggregata] [Edwardsiella tarda] [Shewanella baltica] [Xanthomonas vesicatoria] [Ralstonia solanacearum] [Burkholderia cenocepacia] [Acidovorax avenae]

VLIHDVPETRAELEN----TDDMQ--AALARQAISP-------------IQLQEVPVARGRLRSAHLLVQEPVSQLELLAIPYEEGEPLLPNQPTLWVA IQLQEVPVARGLLRPGHQLVQESASQLDLLGIPYEEGAPLLPGQPTLWVA ISLQEVPVARGELKAGYLLVRESVSQLELLGIPYEKGEHLLPDQEAFWVS VQLQEVPVARGCLRPGWLLVRERAAQLELLAVPHEPAELQVPGEEASWVE IQLQEVPVARGRIESEKLLVTEGNEQIDLLGVPYEKDDDFLPGITSIWVD IVLSEIPVAQGMLRDDAVLVRDTEQNLQALRIAYETGAAFLPDTPTIWVA IMLHEVPIAEGSFKAGHVLAREKAESLKMLNIPFVEEKTFLKGLPPLWVP IMVNEIPVAAGRARPGFFIARETEANLDMFGIPYEVGEAFLPRTKSLWVA LLLYQEPVLSMTLPHLALLD----LAAPTLRQVDHRETLPFGGITLQWIA VLLYQEPVLQLQLAPQEVL-----LIDPIATIPCRVESIPFSNQRLHWIK VLIHDVPETRAELEN----TDDMQ--AALARQAISP-------------VRVHDIPGEPVAVPDGHLLIPDLP--EALRAQAVEAAG-LPNHPAPHWIA ILVLDVPSLTVTIPPGKVMVPGAATDPALAAQCEKAGA-AAGQADSYWAP ILINDVPVAQPQLPGDMLLLLDPQ--SPLATQAQRHGP-LLGMEESLWVP : : : *

442 477 493 476 478 477 471 472 468 451 450 442 461 462 463

HrcV SctV AscV LcrD PcrD EscV BcrD EscV LcrD EsaV SsaV HrcV HrcV HrcV HrcV

[Xanthomonas vesicatoria] [Photorhabdus luminescens] [Aeromonas salmonicida] [Yersinia pestis] [Pseudomonas aeruginosa] [Vibrio harveyi] [Bordetella parapertussis] [Verrucomicrobium spinosum] [Stappia aggregata] [Edwardsiella tarda] [Shewanella baltica] [Xanthomonas vesicatoria] [Ralstonia solanacearum] [Burkholderia cenocepacia] [Acidovorax avenae]

----------------------------LHARAHLFVGIQETQWMLEQVA EAHQERLVKSGLAALSMSQVITWHLSHVLREYAEDFIGVQETRYLLEQME NEHQERLEKSRLATLTTDQVMTWHLSHVLREYAEDFIGIQETRYLLEQME VEYEERLEKSQLEFFSHSQVLTWHLSHVLREYAEDFIGIQETRYLLEQME QAHQDRLERSGCACLGLEQVLTWHLSHVLREYAEDFIGIQETRYLLEQME QSHHEKLQNSHVGFLTPDRILTYHLSHVLKEYAQDFIGIQETRYLLEQME ASLTGALRDAGIPYLGISQILTWHLAYVLKKYSADFIGIQETRFLLSAME AERKVDLDKTNVPYLAPTQVLTYHLSVILKRYAADFVGLQETKYLLEKME MKHQTTAKSAGIQVLTLTSMLTFHLAHVLKSHASEFVGIQETMYVLSQME QEQAEGSAAIGVNRYQGNARIAYLVSLALDRYGAEFIGVQEARYLMDQME GNALEQAEAQGLAVCRDEQIPAYVMGKVIDRYAGEFIGVQETRYLMDAME ----------------------------LHARAHLFVGIQETQWMLEQVA PAHVAQDAALSATGQRVERVIADHVVHVLRRSAHLFVGLQETQWMLERVT ----ERQPPGGATVWRAEQVIANDTIALLRARAKLFVGIQETQWVLEQLG -QAAIPPAQRAGACLEIEQVIAREVVEMLQRHAHLFMGVQEVQWIIERAT : . *:*:**. :::.

464 527 543 526 528 527 521 522 518 501 500 464 511 508 512

HrcV SctV AscV LcrD PcrD EscV BcrD EscV LcrD EsaV SsaV HrcV HrcV HrcV HrcV

[Xanthomonas vesicatoria] [Photorhabdus luminescens] [Aeromonas salmonicida] [Yersinia pestis] [Pseudomonas aeruginosa] [Vibrio harveyi] [Bordetella parapertussis] [Verrucomicrobium spinosum] [Stappia aggregata] [Edwardsiella tarda] [Shewanella baltica] [Xanthomonas vesicatoria] [Ralstonia solanacearum] [Burkholderia cenocepacia] [Acidovorax avenae]

VDYPGLVAEVNKAMPAQRIADVLRRLLEERIPVRNIKSILESLVVWGPKE GSYGELVKEAMRIIPLQRMTEILQRLVGEDISIRNTRTILEAMVVWGQKE GSYSELVKEAQRIIPLQRMTEILQRLVGEDISIRNMRAILEAMVEWGQKE GGYGELIKEVQRIVPLQRMTEILQRLVGEDISIRNMRSILEAMVEWGQKE QGYGELVKEAQRIVPLQRMTEILQRLVGEDISIRNMRAILEAMVEWGQKE SSYSELVKEAQRIVPLQKMTEILQRLVSEDISIRNLRVILEAMVEWGQKE ERFPDLVKECLRVMPVQKIAEILQRLVSEEVSIRNLRAVLEALVEWGQKE GQFAEVVREVQRVLPVQKITEVFQRLVQEEISIRNLRTILQSLIEWGQKE TDFAELVKEVSRVLPVTTIADVLQRLVSEEICIRDTRTILQCLVEWGAKE QRYGELVKELQRQLPVARVADVLQRLVAESVSIRDLRAIFEALVEWAPRE ARYGELVKELQRLLSVGKVAEILQRLVEEGISVRDLRTIFETLVEWVPKE VDYPGLVAEVNKAMPAQRIADVLRRLLEERIPVRNIKSILESLVVWGPKE TDYPGLVAEAQKAVPAQRIADVLRRLLEEQVPIRNMRAILESLVVWGPKE AEFPGLVAEVQKALPLQRIADVLRRLLEENVPIRNARSIMESLVNWGPKE PEYPGLVAEVQKVMPLQRMAEVLRRLLEEQVPIRNIRSIFESLITWAPKE : :: * : :. ::::::**: * : :*: : ::: :: * :*

514 577 593 576 578 577 571 572 568 551 550 514 561 558 562

Page 4 of 5

HrcV SctV AscV LcrD PcrD EscV BcrD EscV LcrD EsaV SsaV HrcV HrcV HrcV HrcV

[Xanthomonas vesicatoria] [Photorhabdus luminescens] [Aeromonas salmonicida] [Yersinia pestis] [Pseudomonas aeruginosa] [Vibrio harveyi] [Bordetella parapertussis] [Verrucomicrobium spinosum] [Stappia aggregata] [Edwardsiella tarda] [Shewanella baltica] [Xanthomonas vesicatoria] [Ralstonia solanacearum] [Burkholderia cenocepacia] [Acidovorax avenae]

KDLLMLTEYVRCDLGRYLAHTATAGTGQLPAVMLDHAVEQLIRQSIRATA KDVVQLTEYIRSSLKRYICYKYANGNNILPAYLLDQQVEEQIRGGIRQTS KDVVQLTEYIRSSLKRYICYKYANGNNILPAYLLDQQVEEQLRGGIRQTS KDVVQLTEYIRSSLKRYICYKYANGNNILPAYLFDQEVEEKIRSGVRQTS KDVVQLTEYIRSSLKRYICYKYSSGNNILPAYLLDQAVEEQIRGGIRQTS KDVVQLTEYIRSSLKRYICYKYASGQNMLPAYLLDQSLEDTIRNGIRQTS KDTVLLTEYVRIALKRYISYKYTSGHNILPAYLLAPKVEETVRAAIRQTA KEAVLLTEYVRSSLRRYISYKFSRGQNLLAVYLLEPSLEESIRKAVRQTS KDPIMLTEHVRTSLSRYITYKFSAGQNIVPAYLLSKGVEDEIRAAVRQTS KDPVMLAEYARLALRRHIIGRQRHGQPWVKAWLIGSHIEQKVRESVRQTS KDIIMLTEYVRIALRRHIRRKFATPQGWIAVLMVGEGIENVIRESIRQST KDLLMLTEYVRCDLGRYLAHTATAGTGQLPAVMLDHAVEQLIRQSIRATA KDTLMLVEYVRGDLGRQIAHQATGGTRQMPAILLDLSVEQTVRQAIKPTP KDMLLLTEYVRGDLSRYIAYSATGGAASLQAVLFTSDLEQHIRQSIKQTP KDLLLLTEYVRSDLGRYLAHEAGGGQRHISAILLAPEIEQAIRGCIKPTP *: : *.*: * * * : : . :. :*: :* :: :.

564 627 643 626 628 627 621 622 618 601 600 564 611 608 612

HrcV SctV AscV LcrD PcrD EscV BcrD EscV LcrD EsaV SsaV HrcV HrcV HrcV HrcV

[Xanthomonas vesicatoria] [Photorhabdus luminescens] [Aeromonas salmonicida] [Yersinia pestis] [Pseudomonas aeruginosa] [Vibrio harveyi] [Bordetella parapertussis] [Verrucomicrobium spinosum] [Stappia aggregata] [Edwardsiella tarda] [Shewanella baltica] [Xanthomonas vesicatoria] [Ralstonia solanacearum] [Burkholderia cenocepacia] [Acidovorax avenae]

AGNFLALPPEQANQLVEQVERIV-GDHAQHP--LAVVASMDVRRYVRRMI AGSYLALDPAVTQSFLEQMKKTVGDLTQMQNK-PVLIVSMDIRRYVRKLI AGSYLALDPTITQSFLDQVRHTVGDLAQMQNK-PVLIVSMDIRRYVRKLI AGSYLALEPAVTESLLEQVRKTIGDLSQIQSK-PVLIVSMDIRRYVRKLI AGSYLALDPAITQAFLERVRQTVGDLAQMQNR-PVLIVSMDIRRYVRKLV AGSYLALDPSVTQQFVSDVKQTIGDLSRMPNK-PVLVVSMDVRRYVRKLI AGSYLALDPDTTRRLVEYIRQCVGDLAAGASR-PVLLTSMDIRRYTRKMI AGAYLALDPGTVKALLQSVRKQVGNIWESNQR-PVLLTSLDVRRYTRKLI GASYLALSPELHRQLVSSIKQTVTDQSLNATQ-PAIIAPMDLRRFVRKIV AGSYSALSQQENQAILTEIHAALERSG--AVN-TAIVTAIDIRRFIRKLI AGSYSALDMAQTQQILTCIQEQLPP----EQT-CVLLTSLDVRRYLRKIV AGNFLALPPEQANQLVEQVERIV-GDHAQHP--LAVVASMDVRRYVRRMI AGNFLTLDPQQVEAIIMRLRGIMQGNPVETPSALAIVTSMDIRRYVRRMI TGNYLALSPDEVRTLAERIGAYVGDVPHDEV---ALVTSMDIRRYVRRMV AGNYLAMSPDDARDITDRIAAVARSSPATGV---ALVTSMDIRRYVKKMI . : :: . : : .::..:*:**: ::::

611 676 692 675 677 676 670 671 667 648 645 611 661 655 659

HrcV SctV AscV LcrD PcrD EscV BcrD EscV LcrD EsaV SsaV HrcV HrcV HrcV HrcV

[Xanthomonas vesicatoria] [Photorhabdus luminescens] [Aeromonas salmonicida] [Yersinia pestis] [Pseudomonas aeruginosa] [Vibrio harveyi] [Bordetella parapertussis] [Verrucomicrobium spinosum] [Stappia aggregata] [Edwardsiella tarda] [Shewanella baltica] [Xanthomonas vesicatoria] [Ralstonia solanacearum] [Burkholderia cenocepacia] [Acidovorax avenae]

EARLTWLQVYSFQELGSEVQLQPIGRVVV----------EGDHHGLPVLSYQELTQQINIQPLGRVCL----------EGDYHALPVLSYQELTQQINIQPLGRVCL----------ESEYYGLPVLSYQELTQQINIQPLGRICL----------ESDYAGLPVLSYQELTQQINIQPLGRIVL----------ESEYYELPVLSFQELTQQINIQPLGRVGM----------EADLYALPVLSYRN-------------------------EQEFYELPVLSHQELTEEITIQPLGRIAT----------ERDFPDLPVLSFQELSADVNIQPLDRISIDDQSALLQMDE ERELFTTAVLSFQELDDEAELKVLGHIELIGDTHAAA--ERELFSTPVLSFQELGDDAEIKVIGQVDLVGDMLDAALAS EARLTWLQVYSFQELGSEVQLQPIGRVVV----------EPHLQALNVYSFQELGGYVDLRPVGKLVL----------EAHLPWLRVYSYQELGDYVELRPLGRVTT----------EGRIDWLRVYSFQELGSLIELRPIGRVA-----------* * *.::

Page 5 of 5

640 705 721 704 706 705 684 700 707 685 685 640 690 684 687