Sep 8, 2014 - Pyruvate. Acetyl-CoA. Acetyl-P. Pi. ADP. ATP. NADH. NAD. Lactate ..... still possible in E. coli Îpta strains, due to the direct oxidation of pyruvate ...
Appl Microbiol Biotechnol DOI 10.1007/s00253-014-6092-x
BIOTECHNOLOGICAL PRODUCTS AND PROCESS ENGINEERING
Metabolic engineering of itaconate production in Escherichia coli Kiira S. Vuoristo & Astrid E. Mars & Jose Vidal Sangra & Jan Springer & Gerrit Eggink & Johan P. M. Sanders & Ruud A. Weusthuis
Received: 12 July 2014 / Revised: 8 September 2014 / Accepted: 10 September 2014 # Springer-Verlag Berlin Heidelberg 2014
Abstract Interest in sustainable development has led to efforts to replace petrochemical-based monomers with biomass-based ones. Itaconic acid, a C5-dicarboxylic acid, is a potential monomer for the chemical industry with many prospective applications. cis-aconitate decarboxylase (CadA) is the key enzyme of itaconate production, converting the citric acid cycle intermediate cis-aconitate into itaconate. Heterologous expression of cadA from Aspergillus terreus in Escherichia coli resulted in low CadA activities and production of trace amounts of itaconate on Luria-Bertani (LB) medium (
